RLG00000003934

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0021497
Physical Location & Seq
Reverse (-)
54427234 .. 54427599
366 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003934

Sequence Viewer

Length: 366 bp
ATGGCACTTGTCCAAGAACGCCGTAAGATCAATAATCTCTGTCTCCCCTTGTCTTTACCCTTCATCTCCGTCCACCACCGCCCAGTTCTCTCTCTTCAAACCATTCCTCGCCCCGCTAGCACCACCACCACCTTCATCTCCTCCTCTACTGCACCCGCTTTGGCCTCAGACCTCGAGCGACTCCAAATCATGGGCCACGGAAGCGGCGGCACCGTCTACAAAGTTTTTCACAAGCCCATTTCCACCACTTACGCTCTCAAACAACTCAAGCCAGCTGCAGACTCCTCCTCCCATAACAACAGACTTCACAGTGAACTCGACATCCACCGGTTCTCCCTACGTCGTCCACTGCCACGCCATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

122

Amino Acids

13.5

Weight (kDa)

10.92

Isoelectric Point (pI)

63.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 59 - 110 5.8e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021497)

Species Orthologous Gene IDs
rosa_laevigata RLG00000003934
rosa_rugosa Rorug07G0040200
rosa_samantha Rh7BG167500 Rh7CG173900 Rh7DG166900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 209
AccB7I CCANNNNNTGG 1 cut(s) 190
AccI GTMKAC 1 cut(s) 216
AciI CCGC 5 cut(s) 79, 114, 156, 204, 207
AfiI CCNNNNNNNGG 1 cut(s) 190
AgeI ACCGGT 1 cut(s) 327
AgsI TTSAA 1 cut(s) 98
AloI GAACNNNNNNTCC 2 cut(s) 306, 338
AluBI AGCT 1 cut(s) 275
AluI AGCT 1 cut(s) 275
Alw26I GTCTC 1 cut(s) 47
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 2 cut(s) 162, 193
ApeKI GCWGC 1 cut(s) 275
AsiGI ACCGGT 1 cut(s) 327
AspS9I GGNCC 1 cut(s) 193
AsuNHI GCTAGC 1 cut(s) 116
AvaI CYCGRG 1 cut(s) 173
BanI GGYRCC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 262
BceAI ACGGC 1 cut(s) 6
BcoDI GTCTC 1 cut(s) 47
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 1 cut(s) 276
BisI GCNGC 3 cut(s) 205, 208, 276
BlsI GCNGC 3 cut(s) 206, 209, 277
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 1 cut(s) 211
BmrI ACTGGG 1 cut(s) 77
BmtI GCTAGC 1 cut(s) 120
BmuI ACTGGG 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 196
BsaWI WCCGGW 1 cut(s) 327
BsaXI ACNNNNNCTCC 4 cut(s) 272, 302, 317, 347
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 196
BseGI GGATG 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 1 cut(s) 83
BseRI GAGGAG 4 cut(s) 130, 133, 274, 277
BseXI GCAGC 1 cut(s) 262
BsgI GTGCAG 1 cut(s) 135
BshFI GGCC 2 cut(s) 164, 195
BshNI GGYRCC 1 cut(s) 209
BshTI ACCGGT 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 190
BsmAI GTCTC 1 cut(s) 47
BsnI GGCC 2 cut(s) 164, 195
BsoBI CYCGRG 1 cut(s) 173
Bsp143I GATC 1 cut(s) 27
BspACI CCGC 5 cut(s) 79, 114, 156, 204, 207
BspANI GGCC 2 cut(s) 164, 195
BspCNI CTCAG 1 cut(s) 179
BspLI GGNNCC 1 cut(s) 211
BspMAI CTGCAG 1 cut(s) 280
BspOI GCTAGC 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 209
BsrFI RCCGGY 1 cut(s) 327
BsrI ACTGG 1 cut(s) 83
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 1 cut(s) 196
BssMI GATC 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 214, 311
Bst6I CTCTTC 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 118, 273
BstDEI CTNAG 1 cut(s) 166
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 1 cut(s) 321
BstKTI GATC 1 cut(s) 30
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 1 cut(s) 27
BstMWI GCNNNNNNNGC 2 cut(s) 117, 201
BstSFI CTRYAG 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 262
BsuRI GGCC 2 cut(s) 164, 195
BtgI CCRYGG 1 cut(s) 196
BtsCI GGATG 1 cut(s) 321
BtsI GCAGTG 1 cut(s) 347
BtsIMutI CAGTG 2 cut(s) 316, 347
Cac8I GCNNGC 2 cut(s) 118, 273
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 1 cut(s) 193
CspAI ACCGGT 1 cut(s) 327
CviAII CATG 1 cut(s) 190
CviJI RGCY 5 cut(s) 164, 195, 235, 271, 275
CviKI_1 RGCY 5 cut(s) 164, 195, 235, 271, 275
DdeI CTNAG 1 cut(s) 166
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 173
FaeI CATG 1 cut(s) 193
FaiI YATR 2 cut(s) 191, 294
FatI CATG 1 cut(s) 189
FauI CCCGC 2 cut(s) 121, 163
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 3 cut(s) 205, 208, 276
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 3 cut(s) 205, 208, 276
FspBI CTAG 1 cut(s) 117
GluI GCNGC 3 cut(s) 205, 208, 276
HaeIII GGCC 2 cut(s) 164, 195
HapII CCGG 1 cut(s) 328
Hin1II CATG 1 cut(s) 193
HinfI GANTC 2 cut(s) 180, 281
HpaII CCGG 1 cut(s) 328
Hpy166II GTNNAC 4 cut(s) 73, 217, 314, 347
Hpy188I TCNGA 1 cut(s) 169
Hpy8I GTNNAC 4 cut(s) 73, 217, 314, 347
Hpy99I CGWCG 1 cut(s) 345
HpyAV CCTTC 2 cut(s) 70, 142
HpyCH4III ACNGT 2 cut(s) 214, 311
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 2 cut(s) 152, 278
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 201
HpyF3I CTNAG 1 cut(s) 166
HpySE526I ACGT 1 cut(s) 340
Hsp92II CATG 1 cut(s) 193
Kzo9I GATC 1 cut(s) 27
LpnPI CCDG 3 cut(s) 96, 285, 341
Lsp1109I GCAGC 1 cut(s) 262
MaeI CTAG 1 cut(s) 117
MaeII ACGT 1 cut(s) 340
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MboII GAAGA 1 cut(s) 86
MlyI GAGTC 2 cut(s) 174, 275
MnlI CCTC 7 cut(s) 117, 151, 154, 175, 182, 295, 298
MspA1I CMGCKG 1 cut(s) 275
MspI CCGG 1 cut(s) 328
MwoI GCNNNNNNNGC 2 cut(s) 117, 201
NdeII GATC 1 cut(s) 27
NheI GCTAGC 1 cut(s) 116
NlaIII CATG 1 cut(s) 193
NlaIV GGNNCC 1 cut(s) 211
PaeR7I CTCGAG 1 cut(s) 173
PflMI CCANNNNNTGG 1 cut(s) 190
PinAI ACCGGT 1 cut(s) 327
PkrI GCNGC 3 cut(s) 206, 209, 277
PleI GAGTC 2 cut(s) 174, 275
PpsI GAGTC 2 cut(s) 174, 275
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 193
PspXI VCTCGAGB 1 cut(s) 173
PstI CTGCAG 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 275
SatI GCNGC 3 cut(s) 205, 208, 276
Sau3AI GATC 1 cut(s) 27
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 2 cut(s) 174, 275
SetI ASST 4 cut(s) 134, 174, 277, 343
SfcI CTRYAG 1 cut(s) 276
Sfr274I CTCGAG 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 173
SmlI CTYRAG 2 cut(s) 173, 266
SmoI CTYRAG 2 cut(s) 173, 266
SsiI CCGC 5 cut(s) 79, 114, 156, 204, 207
SspMI CTAG 1 cut(s) 117
TaaI ACNGT 2 cut(s) 214, 311
TaiI ACGT 1 cut(s) 343
TaqI TCGA 2 cut(s) 174, 318
TauI GCSGC 2 cut(s) 207, 210
TscAI CASTG 2 cut(s) 316, 354
TseI GCWGC 1 cut(s) 275
TspDTI ATGAA 2 cut(s) 52, 124
TspGWI ACGGA 2 cut(s) 58, 213
TspRI CASTG 2 cut(s) 316, 354
Van91I CCANNNNNTGG 1 cut(s) 190
XhoI CTCGAG 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 216
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.