Rorug07G0040200

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0006931
Physical Location & Seq
Reverse (-)
2824829 .. 2828834
4006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0040200.1

Sequence Viewer

Length: 873 bp
ATGAGTGATCACCTGGTGTTGTGTGTTGACCGCTTCACAAAACCTGAAAATTCGCAATCAGTGCAAGGGACTGAGACTCCAGGATCTTCTTCTCTAGAGGCCTCATCCTCCCAATCGGCTGAGATACCCAGCTGTGCGATTGATATCAAGGGAACGGGGGAGAATGTTGTATCAGAAGAAGAGGAGCCACTGATTCAAACTGTTGAATGTCGCATTTGCCAGGAAGAGGATGGCGTTAGCAATTTGGAGGTCCCCTGTGCTTGCAGTGGCAGCTTAAAATATGCTCATAGAAAATGTGTCCAGAGGTGGTGCAATGAGAAGGGAGATATTATTTGTGAAATCTGTCATCAGCCTTACCAACCTGGTTATACTGCCCCATCACCTCCCCCTCAGTCTGACGACACTACTATTGACATCAGTGAGGGCTGGACAATCTCTGGTACCCCCTTGGATTTACGTGATCCTCGACTATTGGCAATGGCAGCGGCAGAACGTCACTTTCTTGAATCTGAATATGATGAGTATGCTGATACAAGTGCTAATGGAGCAGCTTTTTGCCGCTCTGCTGCTCTAATTTTAATGGCTCTTCTACTCCTGAGGCATGCTTTGACTCTTCCCAATGGTGATGGTGATGATGATGATGCTTCTACATTTTTCTCTCTTTTCTTGCTCCGAGCTGCTGGTTTTCTTCTCCCCTGCTACATTATGGCCTGGGCCATCAGCATATTACAACGTCGAAGGCAAAGACAGGAAGCAGCAGCACTTGCGGCAACTGAGGTTGCATTTATGCTGCAGGCAGGGCAACATCGTGGATTACAGTTCACAATAGCTTCAGGACCTGCTGCAACTCCCCATCAGGAGCCAATTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

290

Amino Acids

31.54

Weight (kDa)

4.6

Isoelectric Point (pI)

59.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 70 - 115 3.7e-13 RING-variant domain
DUF3675 PF12428 121 - 240 2.7e-45 Protein of unknown function (DUF3675)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 847
Acc65I GGTACC 1 cut(s) 440
AccB1I GGYRCC 1 cut(s) 440
AccBSI CCGCTC 1 cut(s) 561
AciI CCGC 4 cut(s) 31, 485, 559, 767
AclWI GGATC 2 cut(s) 91, 455
AcsI RAATTY 1 cut(s) 49
AcuI CTGAAG 1 cut(s) 816
AdeI CACNNNGTG 1 cut(s) 16
AfaI GTAC 1 cut(s) 442
AfiI CCNNNNNNNGG 1 cut(s) 226
AgsI TTSAA 4 cut(s) 197, 206, 506, 869
AjnI CCWGG 5 cut(s) 12, 79, 219, 361, 710
AluBI AGCT 5 cut(s) 132, 273, 551, 677, 830
AluI AGCT 5 cut(s) 132, 273, 551, 677, 830
Alw26I GTCTC 1 cut(s) 68
AlwI GGATC 2 cut(s) 91, 455
AlwNI CAGNNNCTG 1 cut(s) 839
AoxI GGCC 3 cut(s) 99, 708, 714
ApeKI GCWGC 9 cut(s) 270, 482, 548, 566, 677, 755, 758, 790, 842
ApoI RAATTY 1 cut(s) 49
Asp718I GGTACC 1 cut(s) 440
AspS9I GGNCC 3 cut(s) 250, 714, 836
AsuHPI GGTGA 3 cut(s) 372, 635, 641
AvaII GGWCC 2 cut(s) 250, 836
AxyI CCTNAGG 1 cut(s) 596
BanI GGYRCC 1 cut(s) 440
BbvI GCAGC 9 cut(s) 282, 494, 553, 560, 664, 767, 770, 777, 829
BccI CCATC 5 cut(s) 224, 385, 620, 725, 861
BciT130I CCWGG 5 cut(s) 14, 81, 221, 363, 712
BclI TGATCA 1 cut(s) 7
BcoDI GTCTC 1 cut(s) 68
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 791
BfuAI ACCTGC 1 cut(s) 847
Bme1390I CCNGG 5 cut(s) 14, 81, 221, 363, 712
Bme18I GGWCC 2 cut(s) 250, 836
BmgT120I GGNCC 3 cut(s) 250, 714, 836
BmiI GGNNCC 4 cut(s) 186, 252, 442, 861
BmrFI CCNGG 5 cut(s) 14, 81, 221, 363, 712
BmsI GCATC 1 cut(s) 631
BpmI CTGGAG 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 458
BsaBI GATNNNNATC 1 cut(s) 143
BsaJI CCNNGG 2 cut(s) 447, 711
BsaXI ACNNNNNCTCC 2 cut(s) 61, 91
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse21I CCTNAGG 1 cut(s) 596
Bse3DI GCAATG 2 cut(s) 319, 483
Bse8I GATNNNNATC 1 cut(s) 143
BseBI CCWGG 5 cut(s) 14, 81, 221, 363, 712
BseDI CCNNGG 2 cut(s) 447, 711
BseGI GGATG 2 cut(s) 104, 235
BseJI GATNNNNATC 1 cut(s) 143
BseLI CCNNNNNNNGG 1 cut(s) 226
BseMI GCAATG 2 cut(s) 319, 483
BseMII CTCAG 5 cut(s) 63, 111, 404, 587, 765
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 9 cut(s) 282, 494, 553, 560, 664, 767, 770, 777, 829
BseYI CCCAGC 1 cut(s) 128
BshFI GGCC 3 cut(s) 101, 710, 716
BshNI GGYRCC 1 cut(s) 440
BslFI GGGAC 2 cut(s) 82, 236
BslI CCNNNNNNNGG 1 cut(s) 226
BsmAI GTCTC 1 cut(s) 68
BsmFI GGGAC 2 cut(s) 82, 236
BsnI GGCC 3 cut(s) 101, 710, 716
Bsp143I GATC 3 cut(s) 7, 83, 460
BspACI CCGC 4 cut(s) 31, 485, 559, 767
BspANI GGCC 3 cut(s) 101, 710, 716
BspCNI CTCAG 5 cut(s) 64, 112, 403, 588, 766
BspLI GGNNCC 4 cut(s) 186, 252, 442, 861
BspMAI CTGCAG 1 cut(s) 795
BspMI ACCTGC 1 cut(s) 847
BspPI GGATC 2 cut(s) 91, 455
BspQI GCTCTTC 1 cut(s) 591
BspT107I GGYRCC 1 cut(s) 440
BsrBI CCGCTC 1 cut(s) 561
BsrDI GCAATG 2 cut(s) 319, 483
BssECI CCNNGG 2 cut(s) 447, 711
BssMI GATC 3 cut(s) 7, 83, 460
BssT1I CCWWGG 1 cut(s) 447
Bst2UI CCWGG 5 cut(s) 14, 81, 221, 363, 712
Bst4CI ACNGT 2 cut(s) 202, 819
Bst6I CTCTTC 4 cut(s) 174, 219, 591, 618
BstAPI GCANNNNNTGC 2 cut(s) 61, 764
BstBAI YACGTR 1 cut(s) 458
BstC8I GCNNGC 3 cut(s) 262, 603, 795
BstDEI CTNAG 5 cut(s) 72, 120, 390, 596, 774
BstF5I GGATG 2 cut(s) 104, 235
BstKTI GATC 3 cut(s) 10, 86, 463
BstMAI GTCTC 1 cut(s) 68
BstMBI GATC 3 cut(s) 7, 83, 460
BstMWI GCNNNNNNNGC 7 cut(s) 61, 270, 482, 545, 764, 767, 799
BstNI CCWGG 5 cut(s) 14, 81, 221, 363, 712
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 5 cut(s) 12, 79, 219, 361, 710
BstSFI CTRYAG 1 cut(s) 791
BstV1I GCAGC 9 cut(s) 282, 494, 553, 560, 664, 767, 770, 777, 829
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
Bsu36I CCTNAGG 1 cut(s) 596
BsuRI GGCC 3 cut(s) 101, 710, 716
BtsCI GGATG 2 cut(s) 104, 235
BtsI GCAGTG 1 cut(s) 271
BtsIMutI CAGTG 4 cut(s) 66, 188, 271, 424
BveI ACCTGC 1 cut(s) 847
Cac8I GCNNGC 3 cut(s) 262, 603, 795
CaiI CAGNNNCTG 1 cut(s) 839
Cfr13I GGNCC 3 cut(s) 250, 714, 836
CsiI ACCWGGT 2 cut(s) 12, 361
Csp6I GTAC 1 cut(s) 441
CviAII CATG 1 cut(s) 602
CviQI GTAC 1 cut(s) 441
DdeI CTNAG 5 cut(s) 72, 120, 390, 596, 774
DpnI GATC 3 cut(s) 9, 85, 462
DpnII GATC 3 cut(s) 7, 83, 460
DraIII CACNNNGTG 1 cut(s) 16
Eam1104I CTCTTC 4 cut(s) 174, 219, 591, 618
EarI CTCTTC 4 cut(s) 174, 219, 591, 618
Eco130I CCWWGG 1 cut(s) 447
Eco147I AGGCCT 1 cut(s) 101
Eco32I GATATC 1 cut(s) 145
Eco47I GGWCC 2 cut(s) 250, 836
Eco57I CTGAAG 1 cut(s) 816
Eco81I CCTNAGG 1 cut(s) 596
EcoO109I RGGNCCY 2 cut(s) 250, 836
EcoRII CCWGG 5 cut(s) 12, 79, 219, 361, 710
EcoRV GATATC 1 cut(s) 145
EcoT14I CCWWGG 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 1 cut(s) 605
FaiI YATR 9 cut(s) 282, 288, 369, 516, 525, 603, 707, 725, 788
FaqI GGGAC 2 cut(s) 82, 236
FatI CATG 1 cut(s) 601
FbaI TGATCA 1 cut(s) 7
FokI GGATG 2 cut(s) 91, 242
FspBI CTAG 1 cut(s) 95
GsaI CCCAGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 3 cut(s) 101, 710, 716
Hin1II CATG 1 cut(s) 605
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 4 cut(s) 76, 193, 506, 610
HphI GGTGA 3 cut(s) 372, 635, 641
Hpy166II GTNNAC 2 cut(s) 28, 822
Hpy188I TCNGA 4 cut(s) 175, 397, 511, 674
Hpy188III TCNNGA 6 cut(s) 95, 301, 503, 595, 834, 857
Hpy8I GTNNAC 2 cut(s) 28, 822
Hpy99I CGWCG 1 cut(s) 738
HpyAV CCTTC 2 cut(s) 313, 732
HpyCH4III ACNGT 2 cut(s) 202, 819
HpyCH4IV ACGT 3 cut(s) 457, 493, 733
HpyCH4V TGCA 6 cut(s) 64, 264, 312, 782, 793, 845
HpyF10VI GCNNNNNNNGC 7 cut(s) 61, 270, 482, 545, 764, 767, 799
HpyF3I CTNAG 5 cut(s) 72, 120, 390, 596, 774
HpySE526I ACGT 3 cut(s) 457, 493, 733
Hsp92II CATG 1 cut(s) 605
KpnI GGTACC 1 cut(s) 444
Ksp22I TGATCA 1 cut(s) 7
Kzo9I GATC 3 cut(s) 7, 83, 460
LguI GCTCTTC 1 cut(s) 591
LmnI GCTCC 4 cut(s) 184, 545, 675, 859
Lsp1109I GCAGC 9 cut(s) 282, 494, 553, 560, 664, 767, 770, 777, 829
LweI GCATC 1 cut(s) 631
MabI ACCWGGT 2 cut(s) 12, 361
MaeI CTAG 1 cut(s) 95
MaeII ACGT 3 cut(s) 457, 493, 733
MaeIII GTNAC 1 cut(s) 494
MalI GATC 3 cut(s) 9, 85, 462
MbiI CCGCTC 1 cut(s) 561
MboI GATC 3 cut(s) 7, 83, 460
MboII GAAGA 8 cut(s) 78, 81, 188, 191, 236, 578, 605, 680
MflI RGATCY 1 cut(s) 83
MluCI AATT 4 cut(s) 49, 241, 573, 864
MlyI GAGTC 2 cut(s) 70, 604
MseI TTAA 2 cut(s) 275, 578
MspA1I CMGCKG 2 cut(s) 132, 485
MspR9I CCNGG 5 cut(s) 14, 81, 221, 363, 712
MvaI CCWGG 5 cut(s) 14, 81, 221, 363, 712
MwoI GCNNNNNNNGC 7 cut(s) 61, 270, 482, 545, 764, 767, 799
NdeII GATC 3 cut(s) 7, 83, 460
NlaIII CATG 1 cut(s) 605
NlaIV GGNNCC 4 cut(s) 186, 252, 442, 861
NmuCI GTSAC 1 cut(s) 494
NspI RCATGY 1 cut(s) 605
PaeI GCATGC 1 cut(s) 605
PceI AGGCCT 1 cut(s) 101
PciSI GCTCTTC 1 cut(s) 591
PcsI WCGNNNNNNNCGW 1 cut(s) 463
PfeI GAWTC 2 cut(s) 193, 506
PfoI TCCNGGA 1 cut(s) 79
PleI GAGTC 2 cut(s) 70, 604
PpsI GAGTC 2 cut(s) 70, 604
Ppu21I YACGTR 1 cut(s) 458
PpuMI RGGWCCY 2 cut(s) 250, 836
Psp5II RGGWCCY 2 cut(s) 250, 836
Psp6I CCWGG 5 cut(s) 12, 79, 219, 361, 710
PspFI CCCAGC 1 cut(s) 128
PspGI CCWGG 5 cut(s) 12, 79, 219, 361, 710
PspN4I GGNNCC 4 cut(s) 186, 252, 442, 861
PspPI GGNCC 3 cut(s) 250, 714, 836
PspPPI RGGWCCY 2 cut(s) 250, 836
PstI CTGCAG 1 cut(s) 795
PstNI CAGNNNCTG 1 cut(s) 839
PsuI RGATCY 1 cut(s) 83
PvuII CAGCTG 1 cut(s) 132
RsaI GTAC 1 cut(s) 442
RsaNI GTAC 1 cut(s) 441
SapI GCTCTTC 1 cut(s) 591
SaqAI TTAA 2 cut(s) 275, 578
Sau3AI GATC 3 cut(s) 7, 83, 460
Sau96I GGNCC 3 cut(s) 250, 714, 836
SchI GAGTC 2 cut(s) 70, 604
ScrFI CCNGG 5 cut(s) 14, 81, 221, 363, 712
SexAI ACCWGGT 2 cut(s) 12, 361
SfaNI GCATC 1 cut(s) 631
SfcI CTRYAG 1 cut(s) 791
SinI GGWCC 2 cut(s) 250, 836
SphI GCATGC 1 cut(s) 605
Sse9I AATT 4 cut(s) 49, 241, 573, 864
SseBI AGGCCT 1 cut(s) 101
SsiI CCGC 4 cut(s) 31, 485, 559, 767
SspMI CTAG 1 cut(s) 95
StuI AGGCCT 1 cut(s) 101
StyD4I CCNGG 5 cut(s) 12, 79, 219, 361, 710
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 2 cut(s) 202, 819
TaiI ACGT 3 cut(s) 460, 496, 736
TaqI TCGA 2 cut(s) 466, 736
TasI AATT 4 cut(s) 49, 241, 573, 864
TauI GCSGC 3 cut(s) 488, 561, 770
TfiI GAWTC 2 cut(s) 193, 506
Tru1I TTAA 2 cut(s) 275, 578
Tru9I TTAA 2 cut(s) 275, 578
TscAI CASTG 4 cut(s) 66, 195, 271, 424
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 9 cut(s) 270, 482, 548, 566, 677, 755, 758, 790, 842
Tsp45I GTSAC 1 cut(s) 494
TspRI CASTG 4 cut(s) 66, 195, 271, 424
VpaK11BI GGWCC 2 cut(s) 250, 836
XapI RAATTY 1 cut(s) 49
XbaI TCTAGA 1 cut(s) 94
XceI RCATGY 1 cut(s) 605
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.