RLG00000004375

High-affinity nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0019972
Physical Location & Seq
Forward (+)
58859165 .. 58859948
784 bp
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UTR
Exon/CDS
Intron
RLM00000004375

Sequence Viewer

Length: 618 bp
ATGGCATTACATGGTCTGATGCTCTTGGCCTCTCTTCTCGTTCTTCTTTCATGCTTAACAGAAACCGCTTATGGAGGCATTCTATTATCTTCTCTGCCCAAAACTCTTAATGTCTCTGCTTCGCCTCACAAACCAGACCAGGTTAATACACCCGGAGTGTTGTTAAATTTTGTTGGAAACATAATAGCCGGAGAAGATAAACTCGGCGTCGTATGGTCTCTGAACAATACGAATTTCCCAGTTGGTACCGACGCGGCCTATAAAACGGTCAACATCAAGCTTTGCTACGCGCCGGTAAGTCAAGTGGGCAAGCCGGGGAGGAAGACGGAGGACGACCTCGACAAGGACAAGACATGCCAGATAGAGTTCTATACTGGACCTTACAACGCCTCTATCAAGTGGCAGTACTACGAGTCGACTTTGGATCGCCACGTGCCTCACGCCTCGTACTTCGTACGCGCCTACGTGAACGACTCTGCTGGTGTTCTGGTGGCTTATGGTCAAAGCACCGGACCGAACAAGGACTACAATATATTTGTGGTTAGCGGAAGCCACAAGAGGCCTAAGATGGTGTCGATTTGGTTCAGTGTCTTCGTTTTGTTCTTCTTTGTTGCTTAG

Protein Analysis

206

Amino Acids

22.52

Weight (kDa)

8.35

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 30 - 183 9.3e-41 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019972)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0192371
rosa_laevigata RLG00000004375
rosa_multiflora Rmu_sc0001559.1_g000012 Rmu_sc0003108.1_g000003
rosa_roxburghii Rroxscaffold_3G00263090
rosa_samantha Rh7DG128500
rosa_wichuraiana Rw7G010730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 245
AccB1I GGYRCC 1 cut(s) 245
AccI GTMKAC 1 cut(s) 416
AccII CGCG 3 cut(s) 254, 290, 459
AciI CCGC 3 cut(s) 66, 254, 546
AclWI GGATC 1 cut(s) 432
AcsI RAATTY 2 cut(s) 166, 232
AcvI CACGTG 1 cut(s) 433
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 4 cut(s) 247, 407, 449, 456
AfiI CCNNNNNNNGG 1 cut(s) 343
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 1 cut(s) 280
AluI AGCT 1 cut(s) 280
Alw26I GTCTC 2 cut(s) 118, 222
AlwI GGATC 1 cut(s) 432
AoxI GGCC 3 cut(s) 27, 255, 560
ApoI RAATTY 2 cut(s) 166, 232
Asp718I GGTACC 1 cut(s) 245
AspLEI GCGC 2 cut(s) 292, 461
AspS9I GGNCC 2 cut(s) 377, 512
AsuC2I CCSGG 2 cut(s) 153, 315
AvaII GGWCC 2 cut(s) 377, 512
BanI GGYRCC 1 cut(s) 245
BbrPI CACGTG 1 cut(s) 433
BbsI GAAGAC 2 cut(s) 329, 583
BccI CCATC 1 cut(s) 562
BciT130I CCWGG 1 cut(s) 140
BcnI CCSGG 2 cut(s) 153, 315
BcoDI GTCTC 2 cut(s) 118, 222
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
BmcAI AGTACT 1 cut(s) 407
Bme1390I CCNGG 3 cut(s) 140, 153, 315
Bme18I GGWCC 2 cut(s) 377, 512
BmgT120I GGNCC 2 cut(s) 377, 512
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 3 cut(s) 140, 153, 315
BmrI ACTGGG 1 cut(s) 233
BmsI GCATC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 233
BpiI GAAGAC 2 cut(s) 329, 583
BpuMI CCSGG 2 cut(s) 153, 315
BsaAI YACGTR 2 cut(s) 433, 466
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 314
BsaWI WCCGGW 1 cut(s) 509
Bsc4I CCNNNNNNNGG 1 cut(s) 343
Bse118I RCCGGY 1 cut(s) 292
Bse1I ACTGG 2 cut(s) 239, 379
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 314
BseLI CCNNNNNNNGG 1 cut(s) 343
BseNI ACTGG 2 cut(s) 239, 379
Bsh1236I CGCG 3 cut(s) 254, 290, 459
BshFI GGCC 3 cut(s) 29, 257, 562
BshNI GGYRCC 1 cut(s) 245
BsiSI CCGG 5 cut(s) 153, 189, 293, 314, 510
BsiWI CGTACG 1 cut(s) 454
BslI CCNNNNNNNGG 1 cut(s) 343
BsmAI GTCTC 2 cut(s) 118, 222
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 3 cut(s) 29, 257, 562
Bso31I GGTCTC 1 cut(s) 222
Bsp143I GATC 1 cut(s) 424
BspACI CCGC 3 cut(s) 66, 254, 546
BspANI GGCC 3 cut(s) 29, 257, 562
BspFNI CGCG 3 cut(s) 254, 290, 459
BspLI GGNNCC 1 cut(s) 247
BspPI GGATC 1 cut(s) 432
BspT107I GGYRCC 1 cut(s) 245
BspTNI GGTCTC 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 292
BsrI ACTGG 2 cut(s) 239, 379
BssAI RCCGGY 1 cut(s) 292
BssECI CCNNGG 1 cut(s) 314
BssMI GATC 1 cut(s) 424
BssNI GRCGYC 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 1 cut(s) 207
BstBAI YACGTR 2 cut(s) 433, 466
BstC8I GCNNGC 1 cut(s) 311
BstDEI CTNAG 2 cut(s) 564, 615
BstENI CCTNNNNNAGG 1 cut(s) 341
BstFNI CGCG 3 cut(s) 254, 290, 459
BstHHI GCGC 2 cut(s) 292, 461
BstKTI GATC 1 cut(s) 427
BstMAI GTCTC 2 cut(s) 118, 222
BstMBI GATC 1 cut(s) 424
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 357
BstSCI CCNGG 3 cut(s) 138, 151, 313
BstUI CGCG 3 cut(s) 254, 290, 459
BstV2I GAAGAC 2 cut(s) 329, 583
BsuRI GGCC 3 cut(s) 29, 257, 562
BtsIMutI CAGTG 1 cut(s) 592
Cac8I GCNNGC 1 cut(s) 311
CfoI GCGC 2 cut(s) 292, 461
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 2 cut(s) 377, 512
CpoI CGGWCCG 1 cut(s) 512
CseI GACGC 2 cut(s) 196, 260
CsiI ACCWGGT 1 cut(s) 138
Csp6I GTAC 4 cut(s) 246, 406, 448, 455
CspI CGGWCCG 1 cut(s) 512
CviAII CATG 3 cut(s) 11, 51, 354
CviJI RGCY 8 cut(s) 29, 188, 257, 280, 313, 494, 552, 562
CviKI_1 RGCY 8 cut(s) 29, 188, 257, 280, 313, 494, 552, 562
CviQI GTAC 4 cut(s) 246, 406, 448, 455
DdeI CTNAG 2 cut(s) 564, 615
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Eco147I AGGCCT 1 cut(s) 562
Eco31I GGTCTC 1 cut(s) 222
Eco47I GGWCC 2 cut(s) 377, 512
Eco72I CACGTG 1 cut(s) 433
EcoNI CCTNNNNNAGG 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 138
FaeI CATG 3 cut(s) 14, 54, 357
FatI CATG 3 cut(s) 10, 50, 353
FblI GTMKAC 1 cut(s) 416
Fnu4HI GCNGC 1 cut(s) 255
Fsp4HI GCNGC 1 cut(s) 255
GlaI GCGC 2 cut(s) 291, 460
GluI GCNGC 1 cut(s) 255
HaeIII GGCC 3 cut(s) 29, 257, 562
HapII CCGG 5 cut(s) 153, 189, 293, 314, 510
HgaI GACGC 2 cut(s) 196, 260
HhaI GCGC 2 cut(s) 292, 461
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 3 cut(s) 14, 54, 357
Hin6I GCGC 2 cut(s) 290, 459
HinP1I GCGC 2 cut(s) 290, 459
HincII GTYRAC 2 cut(s) 271, 417
HindII GTYRAC 2 cut(s) 271, 417
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 2 cut(s) 413, 473
HpaII CCGG 5 cut(s) 153, 189, 293, 314, 510
Hpy166II GTNNAC 3 cut(s) 271, 417, 469
Hpy188I TCNGA 2 cut(s) 18, 222
Hpy8I GTNNAC 3 cut(s) 271, 417, 469
Hpy99I CGWCG 2 cut(s) 212, 254
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 2 cut(s) 432, 465
HpyF3I CTNAG 2 cut(s) 564, 615
HpySE526I ACGT 2 cut(s) 432, 465
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 3 cut(s) 14, 54, 357
HspAI GCGC 2 cut(s) 290, 459
KpnI GGTACC 1 cut(s) 249
Kzo9I GATC 1 cut(s) 424
LweI GCATC 1 cut(s) 9
MabI ACCWGGT 1 cut(s) 138
MaeII ACGT 2 cut(s) 432, 465
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MboII GAAGA 7 cut(s) 26, 35, 81, 206, 334, 583, 595
MluCI AATT 2 cut(s) 166, 232
MlyI GAGTC 2 cut(s) 422, 467
MmeI TCCRAC 1 cut(s) 154
MseI TTAA 4 cut(s) 56, 108, 144, 164
MspI CCGG 5 cut(s) 153, 189, 293, 314, 510
MspR9I CCNGG 3 cut(s) 140, 153, 315
Mva1269I GAATGC 1 cut(s) 78
MvaI CCWGG 1 cut(s) 140
MvnI CGCG 3 cut(s) 254, 290, 459
NciI CCSGG 2 cut(s) 153, 315
NdeII GATC 1 cut(s) 424
NlaIII CATG 3 cut(s) 14, 54, 357
NlaIV GGNNCC 1 cut(s) 247
NmeAIII GCCGAG 1 cut(s) 183
NspI RCATGY 1 cut(s) 357
PceI AGGCCT 1 cut(s) 562
PctI GAATGC 1 cut(s) 78
Pfl23II CGTACG 1 cut(s) 454
PkrI GCNGC 1 cut(s) 256
PleI GAGTC 2 cut(s) 421, 467
PmaCI CACGTG 1 cut(s) 433
PmlI CACGTG 1 cut(s) 433
PpsI GAGTC 2 cut(s) 421, 467
Ppu21I YACGTR 2 cut(s) 433, 466
Psp6I CCWGG 1 cut(s) 138
PspCI CACGTG 1 cut(s) 433
PspGI CCWGG 1 cut(s) 138
PspLI CGTACG 1 cut(s) 454
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 2 cut(s) 377, 512
PsrI GAACNNNNNNTAC 2 cut(s) 509, 541
RsaI GTAC 4 cut(s) 247, 407, 449, 456
RsaNI GTAC 4 cut(s) 246, 406, 448, 455
Rsr2I CGGWCCG 1 cut(s) 512
RsrII CGGWCCG 1 cut(s) 512
SalI GTCGAC 1 cut(s) 415
SaqAI TTAA 4 cut(s) 56, 108, 144, 164
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 1 cut(s) 424
Sau96I GGNCC 2 cut(s) 377, 512
ScaI AGTACT 1 cut(s) 407
SchI GAGTC 2 cut(s) 422, 467
ScrFI CCNGG 3 cut(s) 140, 153, 315
SetI ASST 6 cut(s) 144, 282, 339, 382, 435, 468
SexAI ACCWGGT 1 cut(s) 138
SfaNI GCATC 1 cut(s) 9
SinI GGWCC 2 cut(s) 377, 512
Sse9I AATT 2 cut(s) 166, 232
SseBI AGGCCT 1 cut(s) 562
SsiI CCGC 3 cut(s) 66, 254, 546
StuI AGGCCT 1 cut(s) 562
StyD4I CCNGG 3 cut(s) 138, 151, 313
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 2 cut(s) 435, 468
TaqI TCGA 3 cut(s) 339, 416, 575
TaqII GACCGA 1 cut(s) 529
TasI AATT 2 cut(s) 166, 232
TatI WGTACW 1 cut(s) 405
TauI GCSGC 1 cut(s) 257
Tru1I TTAA 4 cut(s) 56, 108, 144, 164
Tru9I TTAA 4 cut(s) 56, 108, 144, 164
TscAI CASTG 1 cut(s) 592
TspDTI ATGAA 1 cut(s) 39
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 1 cut(s) 592
VpaK11BI GGWCC 2 cut(s) 377, 512
XagI CCTNNNNNAGG 1 cut(s) 341
XapI RAATTY 2 cut(s) 166, 232
XceI RCATGY 1 cut(s) 357
XmiI GTMKAC 1 cut(s) 416
ZrmI AGTACT 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.