Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0015463

Physical Location & Seq

Genomic Coordinates Reverse (-)

63363641 .. 63370781

Gene Structure 7141 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004882

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2322 bp

ATGGCCGCTAAACGCTTTTTAACTGATTCAGAATCCGACCAAGATCAGCCCAGTGATAACAACAGGAAGAGACCAAGACTTTCCTTCGCTTCTGTGATTGGAGAAGTGGTTATGGTCAACTCCATGCAAAACCTTTTCTCATCCTTGGAGCCTTTACTTAGAAGAGTGGTGAGTGAAGAGGTTGATCAAGTTCTAAGGCGATGCTCTCGCTCTTTAACAAGGTCACCTTCACTGAGAATCCAAGCGCTCGAGCCATCGAGCTTATATCTAGTCTTTGCCAAGAGCCTCTCTCTCCCAATTTTCACTGCAAGCAAGATATCAGACGAAGACAACAACCAAATTCAAGTCCTTGTAGTGGACAAGAACATGATCAGTGGAAGCCACCAAATAGGTCCGATCTCTCTTCCACATCCGGTCAAAGTAGAAATCGTTGTTCTTGACGGTGACTTCCCTAGTGGAGATCGGGAGCACTGGACAAGCCAGGAATTCGACAACAATATTTTGAAGGAGAGAACAGGCAAGAGGCCGTTGCTCACCGGAGACGTGAATGTTACGGTGAGAGATGGGTTTGCAACGATCGGGGACATTGAGTTTACAGACAATTCGAGTTGGATCCGGAGCAGGAAGTTTAGGCTTGGTGCAAGGGTGGCTCCGGGGACGGGGTATCAGGGTTCGAGGATTCGGGAAGCCATGACTGATGCTTTCGTTGTCAAAGATCATCGTGGAGAGTTGTACAAGAAGCATCATCCACCAATGTTGGAAGACGAAGTTTGGCGGCTGGAGAAGATTGGGAAGGATGGAGCTTTTCACAGGAAGCTAGCTGCGGACAACATTCATACAGTTCAAGACTTCTTGAAGATGTTTGTCGTCGACCGATCGAAGCTTAAAAAGATATTAGGGATTGGAATGTCAGAAAAGATGTGGGAAGTAACAGTGAAGCATGCAAGAACATGCGTGATGGGTAACAAAATGTACATTTTTCGGGGTCCTCATTTCGAGCTTTTCTTATCTCCGATATGTCAAGTGATGAAGGCTGTGATCAATGGCCAGACTTTCTCTGCTCGGGACCTAAGCAATTATAATAGGCCGAACTATATTGAGAACTTGGTAAGGCAAGCGCATGCAAACTGGAATTCCTTGGAGGCGGTTGATACAATGTCGAATGAGACTGCCTTACTTACACAAGAGGACCAACACTATCCTCAGGCAATGATGGTAAGAGCATTGGATCATCAAGTGATCAACCATGGGCAGCAGCAGCAGCTAGCTGATCATAAGTGTATCAATAATGTTGGAGCTGCTGGTTATCTACAGAGCAACACCATGATGGGGTTTGAGTGCAGTGATAATTGGCAGATCAGTTCACCATACTCGTCGTTCAGCCCATTGATGGATACTGGTGTCATCAAGTGTAGCACCTCAGCAGACTCATCATCTTCAGATGGGGATTTAGGATCTCAGGTCATCAAACTTAAATCACCGAATCATTTTCTCCACCAGCTTATTCTACGGATGACTATGCCTAAGCATAATCCTCTCTTCCCCATTTTCTTCATATTCTACTACTTCACCTTCCCCTCATGCCATGCCCAATACACACAGTGCAAGCAACCTTTCAACTGTGGTAACCTCACAAACATATCCTACCCTTTCTGGGGAGGACCCAATCGCCCCCAAGAGTGCGATCGCTTTGGCTTCGAGCTTATCAACTGTGAAGAAGAAGATCAACCCCCTCGCATAAAAATCGAAGAGCTAGATTTCCATGTGTCCAACATCAACAACCAAGATCTTCTTCACACCATGACAATCTCTCGCTCCGACCTCTGGGACAGTCCTTGTACTGATCTCCTCGTCAACACCACTCTCGACTACAACCGTTTCTCTTATGTCCATACAGTTCGTAACCTCACCTTGTACTACAGCTGCCTCCCTCAAAACGAATCCGTCCTCAACAGTTTTACGTGCAAAATAGACGGCATATCCAAGGACAGAGATATTGCGTACTACCTGGATGATTCAATTTCAAGAGACGTGAATCGGACGTCGTGTCCTACCAAAATCCGAGTCCCCATTTTATCGGATGGCTTTGATGTTATGTCTGAGAATGCAACGGAAGCAGTGGAAAAGGTTTTGAGACAAGGGTTTCAGGTTGAGTACAGTGCGGAATGGGAGCTCTGTAGGTCATGCGTGAACTCTCATGGCATCTGTGGATCGAACTCAACGACGGATTCGTTTCTGTGCCTTTGCGGGGACCGACCTTACAGTTCTACTTGTCGAAGGGATTCGAGGTATGCTCAGTCATGTTTATTCCTCATCTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002229 GO:0002237 GO:0002239 GO:0002682 GO:0002831 GO:0002833 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009889 GO:0009987 GO:0010033 GO:0010112 GO:0010224 GO:0010337 GO:0010468 GO:0010556 GO:0010565 GO:0010817 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031347 GO:0031349 GO:0032101 GO:0032103 GO:0032350 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043900 GO:0043902 GO:0044424 GO:0044464 GO:0045088 GO:0046885 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0071216 GO:0071219 GO:0080090 GO:0080134 GO:0080142 GO:0097159 GO:0098542 GO:0140110 GO:1900424 GO:1900426 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Calmodulin_bind

Protein Analysis

774

Amino Acids

87.87

Weight (kDa)

6.23

Isoelectric Point (pI)

50.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calmodulin_bind	PF07887	89 - 240	9.9e-58	Calmodulin binding protein-like N-terminal domain
Calmod_bind_M	PF20451	252 - 318	4.7e-33	Calmodulin binding protein central domain
Calmod_bind_C	PF20452	323 - 382	5.8e-17	Calmodulin binding protein C-terminal domain
GUB_WAK_bind	PF13947	535 - 596	4e-11	Wall-associated receptor kinase galacturonan-binding
WAK_assoc	PF14380	674 - 751	4.1e-16	Wall-associated receptor kinase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0015463)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73805
fragaria_vesca	FvH4_5g11800
malus_domestica	MD14G1228600.v1.1
prunus_persica	Prupe.5G223600_v2.0.a1
pyrus_communis	pycom06g19470 pycom14g18990
rosa_chinensis	RchiOBHm_Chr7g0185801
rosa_laevigata	RLG00000004882
rosa_multiflora	Rmu_sc0002121.1_g000002
rosa_roxburghii	Rroxscaffold_3G00268620
rosa_rugosa	Rorug06G0471400.1
rosa_samantha	Rh7AG070900 Rh7BG076900 Rh7CG076900 Rh7DG076800
rosa_wichuraiana	Rw7G006410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1080
AatII	GACGTC	1 cut(s)	2045
AccI	GTMKAC	1 cut(s)	870
AccIII	TCCGGA	1 cut(s)	615
AciI	CCGC	6 cut(s)	6, 775, 824, 1145, 2162, 2247
AclWI	GGATC	5 cut(s)	607, 620, 1236, 1462, 2218
AcoI	YGGCCR	2 cut(s)	3, 1045
AcsI	RAATTY	3 cut(s)	339, 485, 1132
AcuI	CTGAAG	1 cut(s)	1422
AcyI	GRCGYC	1 cut(s)	2042
AdeI	CACNNNGTG	1 cut(s)	1602
AfaI	GTAC	6 cut(s)	734, 974, 1840, 1916, 2003, 2156
AfeI	AGCGCT	1 cut(s)	246
AfiI	CCNNNNNNNGG	8 cut(s)	355, 659, 1329, 1390, 1654, 1655, 1824, 2248
AgsI	TTSAA	7 cut(s)	344, 505, 845, 856, 1618, 2019, 2025
AhdI	GACNNNNNGTC	1 cut(s)	2046
AjiI	CACGTC	2 cut(s)	544, 2032
AjnI	CCWGG	2 cut(s)	480, 2007
AjuI	GAANNNNNNNTTGG	2 cut(s)	1776, 1808
Alw21I	GWGCWC	2 cut(s)	471, 2175
Alw26I	GTCTC	5 cut(s)	64, 534, 1160, 2022, 2128
AlwI	GGATC	5 cut(s)	607, 620, 1236, 1462, 2218
Ama87I	CYCGRG	2 cut(s)	248, 1062
Aor13HI	TCCGGA	1 cut(s)	615
Aor51HI	AGCGCT	1 cut(s)	246
AoxI	GGCC	4 cut(s)	3, 524, 1045, 1085
ApeKI	GCWGC	7 cut(s)	821, 1252, 1255, 1258, 1261, 1298, 1923
ApoI	RAATTY	3 cut(s)	339, 485, 1132
ArsI	GACNNNNNNTTYG	2 cut(s)	2049, 2081
AsiSI	GCGATCGC	1 cut(s)	1687
Asp700I	GAANNNNTTC	1 cut(s)	2281
AspLEI	GCGC	2 cut(s)	247, 1120
AspS9I	GGNCC	6 cut(s)	392, 986, 1066, 1189, 1661, 2251
AsuC2I	CCSGG	1 cut(s)	654
AsuHPI	GGTGA	9 cut(s)	181, 216, 455, 526, 568, 1356, 1470, 1561, 1900
AsuNHI	GCTAGC	2 cut(s)	817, 1264
AvaI	CYCGRG	2 cut(s)	248, 1062
AvaII	GGWCC	6 cut(s)	392, 986, 1066, 1189, 1661, 2251
AxyI	CCTNAGG	1 cut(s)	1203
BaeI	ACNNNNGTAYC	2 cut(s)	1386, 1419
BalI	TGGCCA	1 cut(s)	1047
BamHI	GGATCC	1 cut(s)	612
BanII	GRGCYC	1 cut(s)	2175
BbsI	GAAGAC	2 cut(s)	333, 768
Bbv12I	GWGCWC	2 cut(s)	471, 2175
BbvCI	CCTCAGC	1 cut(s)	1420
BbvI	GCAGC	7 cut(s)	808, 1264, 1267, 1270, 1273, 1285, 1910
BccI	CCATC	9 cut(s)	262, 557, 791, 952, 1207, 1321, 1384, 1436, 2075
BceAI	ACGGC	2 cut(s)	511, 1990
BcgI	CGANNNNNNTGC	2 cut(s)	1726, 1760
BciT130I	CCWGG	2 cut(s)	482, 2009
BciVI	GTATCC	1 cut(s)	1387
BclI	TGATCA	5 cut(s)	184, 369, 1038, 1239, 1270
BcnI	CCSGG	1 cut(s)	654
BcoDI	GTCTC	5 cut(s)	64, 534, 1160, 2022, 2128
BfaI	CTAG	5 cut(s)	269, 453, 818, 1265, 1754
BfmI	CTRYAG	3 cut(s)	1310, 1918, 2176
BfoI	RGCGCY	1 cut(s)	248
BfuI	GTATCC	1 cut(s)	1387
BglII	AGATCT	1 cut(s)	1786
BisI	GCNGC	9 cut(s)	6, 776, 822, 1253, 1256, 1259, 1262, 1299, 1924
BlsI	GCNGC	9 cut(s)	7, 777, 823, 1254, 1257, 1260, 1263, 1300, 1925
Bme1390I	CCNGG	3 cut(s)	482, 654, 2009
Bme18I	GGWCC	6 cut(s)	392, 986, 1066, 1189, 1661, 2251
BmeRI	GACNNNNNGTC	1 cut(s)	2046
BmeT110I	CYCGRG	2 cut(s)	248, 1062
BmgBI	CACGTC	2 cut(s)	544, 2032
BmgT120I	GGNCC	6 cut(s)	392, 986, 1066, 1189, 1661, 2251
BmiI	GGNNCC	7 cut(s)	150, 614, 651, 987, 1067, 1663, 2252
BmrFI	CCNGG	3 cut(s)	482, 654, 2009
BmrI	ACTGGG	1 cut(s)	45
BmsI	GCATC	4 cut(s)	191, 688, 751, 2211
BmtI	GCTAGC	2 cut(s)	821, 1268
BmuI	ACTGGG	1 cut(s)	45
BpiI	GAAGAC	2 cut(s)	333, 768
BplI	GAGNNNNNCTC	4 cut(s)	274, 306, 2278, 2310
BpmI	CTGGAG	1 cut(s)	800
Bpu10I	CCTNAGC	3 cut(s)	1070, 1420, 1524
BpuMI	CCSGG	1 cut(s)	654
BsaAI	YACGTR	1 cut(s)	1962
BsaHI	GRCGYC	1 cut(s)	2042
BsaI	GGTCTC	1 cut(s)	64
BsaJI	CCNNGG	5 cut(s)	144, 653, 1137, 1246, 1983
BsaWI	WCCGGW	3 cut(s)	412, 536, 615
BsaXI	ACNNNNNCTCC	2 cut(s)	93, 123
Bsc4I	CCNNNNNNNGG	8 cut(s)	355, 659, 1329, 1390, 1654, 1655, 1824, 2248
Bse1I	ACTGG	4 cut(s)	51, 476, 1133, 1402
Bse21I	CCTNAGG	1 cut(s)	1203
Bse3DI	GCAATG	1 cut(s)	1215
BseAI	TCCGGA	1 cut(s)	615
BseBI	CCWGG	2 cut(s)	482, 2009
BseDI	CCNNGG	5 cut(s)	144, 653, 1137, 1246, 1983
BseGI	GGATG	7 cut(s)	140, 409, 745, 802, 1518, 2017, 2086
BseLI	CCNNNNNNNGG	8 cut(s)	355, 659, 1329, 1390, 1654, 1655, 1824, 2248
BseMI	GCAATG	1 cut(s)	1215
BseMII	CTCAG	6 cut(s)	224, 1217, 1434, 1472, 2091, 2309
BseNI	ACTGG	4 cut(s)	51, 476, 1133, 1402
BseRI	GAGGAG	1 cut(s)	1838
BseXI	GCAGC	7 cut(s)	808, 1264, 1267, 1270, 1273, 1285, 1910
BsgI	GTGCAG	1 cut(s)	1360
Bsh1285I	CGRYCG	4 cut(s)	579, 874, 878, 1687
BshFI	GGCC	4 cut(s)	5, 526, 1047, 1087
BsiEI	CGRYCG	4 cut(s)	579, 874, 878, 1687
BsiHKAI	GWGCWC	2 cut(s)	471, 2175
BsiHKCI	CYCGRG	2 cut(s)	248, 1062
BsiSI	CCGG	4 cut(s)	413, 537, 616, 653
BslFI	GGGAC	6 cut(s)	596, 670, 1079, 1841, 2051, 2264
BslI	CCNNNNNNNGG	8 cut(s)	355, 659, 1329, 1390, 1654, 1655, 1824, 2248
BsmAI	GTCTC	5 cut(s)	64, 534, 1160, 2022, 2128
BsmBI	CGTCTC	2 cut(s)	534, 2022
BsmFI	GGGAC	6 cut(s)	596, 670, 1079, 1841, 2051, 2264
BsmI	GAATGC	1 cut(s)	2110
BsnI	GGCC	4 cut(s)	5, 526, 1047, 1087
Bso31I	GGTCTC	1 cut(s)	64
BsoBI	CYCGRG	2 cut(s)	248, 1062
Bsp1286I	GDGCHC	2 cut(s)	471, 2175
Bsp13I	TCCGGA	1 cut(s)	615
Bsp1407I	TGTACA	2 cut(s)	732, 972
Bsp19I	CCATGG	1 cut(s)	1246
BspACI	CCGC	6 cut(s)	6, 775, 824, 1145, 2162, 2247
BspANI	GGCC	4 cut(s)	5, 526, 1047, 1087
BspCNI	CTCAG	6 cut(s)	225, 1216, 1433, 1471, 2092, 2308
BspEI	TCCGGA	1 cut(s)	615
BspLI	GGNNCC	7 cut(s)	150, 614, 651, 987, 1067, 1663, 2252
BspOI	GCTAGC	2 cut(s)	821, 1268
BspPI	GGATC	5 cut(s)	607, 620, 1236, 1462, 2218
BspQI	GCTCTTC	1 cut(s)	1743
BspTNI	GGTCTC	1 cut(s)	64
BsrDI	GCAATG	1 cut(s)	1215
BsrGI	TGTACA	2 cut(s)	732, 972
BsrI	ACTGG	4 cut(s)	51, 476, 1133, 1402
BssECI	CCNNGG	5 cut(s)	144, 653, 1137, 1246, 1983
BssNI	GRCGYC	1 cut(s)	2042
BssT1I	CCWWGG	4 cut(s)	144, 1137, 1246, 1983
Bst2UI	CCWGG	2 cut(s)	482, 2009
Bst6I	CTCTTC	6 cut(s)	62, 157, 171, 408, 1544, 1743
BstACI	GRCGYC	1 cut(s)	2042
BstAUI	TGTACA	2 cut(s)	732, 972
BstBAI	YACGTR	1 cut(s)	1962
BstC8I	GCNNGC	7 cut(s)	310, 819, 942, 1116, 1122, 1266, 1607
BstDSI	CCRYGG	1 cut(s)	1246
BstEII	GGTNACC	2 cut(s)	222, 1625
BstF5I	GGATG	7 cut(s)	140, 409, 745, 802, 1518, 2017, 2086
BstH2I	RGCGCY	1 cut(s)	248
BstHHI	GCGC	2 cut(s)	247, 1120
BstMAI	GTCTC	5 cut(s)	64, 534, 1160, 2022, 2128
BstMCI	CGRYCG	4 cut(s)	579, 874, 878, 1687
BstMWI	GCNNNNNNNGC	4 cut(s)	647, 1258, 1261, 2114
BstNI	CCWGG	2 cut(s)	482, 2009
BstNSI	RCATGY	3 cut(s)	944, 954, 1124
BstPI	GGTNACC	2 cut(s)	222, 1625
BstSCI	CCNGG	3 cut(s)	480, 652, 2007
BstSFI	CTRYAG	3 cut(s)	1310, 1918, 2176
BstV1I	GCAGC	7 cut(s)	808, 1264, 1267, 1270, 1273, 1285, 1910
BstV2I	GAAGAC	2 cut(s)	333, 768
BstX2I	RGATCY	3 cut(s)	612, 1454, 1786
BstYI	RGATCY	3 cut(s)	612, 1454, 1786
Bsu36I	CCTNAGG	1 cut(s)	1203
BsuI	GTATCC	1 cut(s)	1387
BsuRI	GGCC	4 cut(s)	5, 526, 1047, 1087
BtgI	CCRYGG	1 cut(s)	1246
BtgZI	GCGATG	1 cut(s)	214
BtrI	CACGTC	2 cut(s)	544, 2032
BtsCI	GGATG	7 cut(s)	140, 409, 745, 802, 1518, 2017, 2086
BtsI	GCAGTG	3 cut(s)	303, 1348, 2124
Cac8I	GCNNGC	7 cut(s)	310, 819, 942, 1116, 1122, 1266, 1607
CfoI	GCGC	2 cut(s)	247, 1120
Cfr13I	GGNCC	6 cut(s)	392, 986, 1066, 1189, 1661, 2251
Csp6I	GTAC	6 cut(s)	733, 973, 1839, 1915, 2002, 2155
CspCI	CAANNNNNGTGG	2 cut(s)	738, 773
CviQI	GTAC	6 cut(s)	733, 973, 1839, 1915, 2002, 2155
DraIII	CACNNNGTG	1 cut(s)	1602
DriI	GACNNNNNGTC	1 cut(s)	2046
EaeI	YGGCCR	2 cut(s)	3, 1045
Eam1104I	CTCTTC	6 cut(s)	62, 157, 171, 408, 1544, 1743
Eam1105I	GACNNNNNGTC	1 cut(s)	2046
EarI	CTCTTC	6 cut(s)	62, 157, 171, 408, 1544, 1743
Ecl136II	GAGCTC	1 cut(s)	2173
Eco130I	CCWWGG	4 cut(s)	144, 1137, 1246, 1983
Eco24I	GRGCYC	1 cut(s)	2175
Eco31I	GGTCTC	1 cut(s)	64
Eco32I	GATATC	1 cut(s)	318
Eco47I	GGWCC	6 cut(s)	392, 986, 1066, 1189, 1661, 2251
Eco47III	AGCGCT	1 cut(s)	246
Eco53kI	GAGCTC	1 cut(s)	2173
Eco57I	CTGAAG	1 cut(s)	1422
Eco81I	CCTNAGG	1 cut(s)	1203
Eco88I	CYCGRG	2 cut(s)	248, 1062
Eco91I	GGTNACC	2 cut(s)	222, 1625
EcoICRI	GAGCTC	1 cut(s)	2173
EcoO109I	RGGNCCY	3 cut(s)	986, 1066, 1661
EcoO65I	GGTNACC	2 cut(s)	222, 1625
EcoRI	GAATTC	2 cut(s)	485, 1132
EcoRII	CCWGG	2 cut(s)	480, 2007
EcoRV	GATATC	1 cut(s)	318
EcoT14I	CCWWGG	4 cut(s)	144, 1137, 1246, 1983
EcoT38I	GRGCYC	1 cut(s)	2175
ErhI	CCWWGG	4 cut(s)	144, 1137, 1246, 1983
Esp3I	CGTCTC	2 cut(s)	534, 2022
FalI	AAGNNNNNCTT	4 cut(s)	211, 243, 1776, 1808
FaqI	GGGAC	6 cut(s)	596, 670, 1079, 1841, 2051, 2264
FauI	CCCGC	1 cut(s)	2240
FbaI	TGATCA	5 cut(s)	184, 369, 1038, 1239, 1270
FblI	GTMKAC	1 cut(s)	870
Fnu4HI	GCNGC	9 cut(s)	6, 776, 822, 1253, 1256, 1259, 1262, 1299, 1924
FokI	GGATG	7 cut(s)	127, 396, 732, 809, 1525, 2024, 2093
FriOI	GRGCYC	1 cut(s)	2175
Fsp4HI	GCNGC	9 cut(s)	6, 776, 822, 1253, 1256, 1259, 1262, 1299, 1924
FspBI	CTAG	5 cut(s)	269, 453, 818, 1265, 1754
GlaI	GCGC	2 cut(s)	246, 1119
GluI	GCNGC	9 cut(s)	6, 776, 822, 1253, 1256, 1259, 1262, 1299, 1924
GsuI	CTGGAG	1 cut(s)	800
HaeII	RGCGCY	1 cut(s)	248
HaeIII	GGCC	4 cut(s)	5, 526, 1047, 1087
HapII	CCGG	4 cut(s)	413, 537, 616, 653
HhaI	GCGC	2 cut(s)	247, 1120
Hin1I	GRCGYC	1 cut(s)	2042
Hin6I	GCGC	2 cut(s)	245, 1118
HinP1I	GCGC	2 cut(s)	245, 1118
HincII	GTYRAC	3 cut(s)	118, 871, 1855
HindII	GTYRAC	3 cut(s)	118, 871, 1855
HindIII	AAGCTT	1 cut(s)	881
HpaII	CCGG	4 cut(s)	413, 537, 616, 653
HphI	GGTGA	9 cut(s)	181, 216, 455, 526, 568, 1356, 1470, 1561, 1900
Hpy166II	GTNNAC	7 cut(s)	118, 358, 594, 871, 1364, 1855, 2191
Hpy188III	TCNNGA	9 cut(s)	437, 464, 616, 683, 845, 853, 1064, 1865, 2025
Hpy8I	GTNNAC	7 cut(s)	118, 358, 594, 871, 1364, 1855, 2191
Hpy99I	CGWCG	4 cut(s)	872, 1378, 2047, 2227
HpyAV	CCTTC	7 cut(s)	94, 237, 499, 787, 1024, 1582, 2271
HpyCH4IV	ACGT	4 cut(s)	543, 1961, 2031, 2042
HpyF10VI	GCNNNNNNNGC	4 cut(s)	647, 1258, 1261, 2114
HpySE526I	ACGT	4 cut(s)	543, 1961, 2031, 2042
Hsp92I	GRCGYC	1 cut(s)	2042
HspAI	GCGC	2 cut(s)	245, 1118
Kpn2I	TCCGGA	1 cut(s)	615
Ksp22I	TGATCA	5 cut(s)	184, 369, 1038, 1239, 1270
LguI	GCTCTTC	1 cut(s)	1743
LmnI	GCTCC	8 cut(s)	148, 466, 618, 655, 800, 1295, 1820, 2170
Lsp1109I	GCAGC	7 cut(s)	808, 1264, 1267, 1270, 1273, 1285, 1910
LweI	GCATC	4 cut(s)	191, 688, 751, 2211
MaeI	CTAG	5 cut(s)	269, 453, 818, 1265, 1754
MaeII	ACGT	4 cut(s)	543, 1961, 2031, 2042
MaeIII	GTNAC	7 cut(s)	222, 443, 550, 928, 962, 1625, 1901
MflI	RGATCY	3 cut(s)	612, 1454, 1786
MhlI	GDGCHC	2 cut(s)	471, 2175
MlsI	TGGCCA	1 cut(s)	1047
MluCI	AATT	8 cut(s)	297, 339, 485, 601, 1075, 1132, 1348, 2019
MluNI	TGGCCA	1 cut(s)	1047
MlyI	GAGTC	2 cut(s)	1421, 2073
MmeI	TCCRAC	6 cut(s)	60, 590, 738, 1273, 1794, 1842
Mox20I	TGGCCA	1 cut(s)	1047
MroI	TCCGGA	1 cut(s)	615
MroXI	GAANNNNTTC	1 cut(s)	2281
MscI	TGGCCA	1 cut(s)	1047
MseI	TTAA	4 cut(s)	20, 215, 885, 1473
MslI	CAYNNNNRTG	1 cut(s)	1325
Msp20I	TGGCCA	1 cut(s)	1047
MspA1I	CMGCKG	1 cut(s)	1923
MspI	CCGG	4 cut(s)	413, 537, 616, 653
MspR9I	CCNGG	3 cut(s)	482, 654, 2009
Mva1269I	GAATGC	1 cut(s)	2110
MvaI	CCWGG	2 cut(s)	482, 2009
MwoI	GCNNNNNNNGC	4 cut(s)	647, 1258, 1261, 2114
NciI	CCSGG	1 cut(s)	654
NcoI	CCATGG	1 cut(s)	1246
NheI	GCTAGC	2 cut(s)	817, 1264
NlaIV	GGNNCC	7 cut(s)	150, 614, 651, 987, 1067, 1663, 2252
NmuCI	GTSAC	2 cut(s)	222, 443
NspI	RCATGY	3 cut(s)	944, 954, 1124
PaeI	GCATGC	2 cut(s)	944, 1124
PaeR7I	CTCGAG	1 cut(s)	248
PciSI	GCTCTTC	1 cut(s)	1743
PcsI	WCGNNNNNNNCGW	2 cut(s)	2219, 2228
PctI	GAATGC	1 cut(s)	2110
PdmI	GAANNNNTTC	1 cut(s)	2281
PkrI	GCNGC	9 cut(s)	7, 777, 823, 1254, 1257, 1260, 1263, 1300, 1925
Ple19I	CGATCG	3 cut(s)	579, 878, 1687
PleI	GAGTC	2 cut(s)	1421, 2072
PpsI	GAGTC	2 cut(s)	1421, 2072
Ppu21I	YACGTR	1 cut(s)	1962
PpuMI	RGGWCCY	3 cut(s)	986, 1066, 1661
PsiI	TTATAA	1 cut(s)	1080
Psp124BI	GAGCTC	1 cut(s)	2175
Psp5II	RGGWCCY	3 cut(s)	986, 1066, 1661
Psp6I	CCWGG	2 cut(s)	480, 2007
PspEI	GGTNACC	2 cut(s)	222, 1625
PspGI	CCWGG	2 cut(s)	480, 2007
PspN4I	GGNNCC	7 cut(s)	150, 614, 651, 987, 1067, 1663, 2252
PspPI	GGNCC	6 cut(s)	392, 986, 1066, 1189, 1661, 2251
PspPPI	RGGWCCY	3 cut(s)	986, 1066, 1661
PspXI	VCTCGAGB	1 cut(s)	248
PsuI	RGATCY	3 cut(s)	612, 1454, 1786
PvuI	CGATCG	3 cut(s)	579, 878, 1687
PvuII	CAGCTG	1 cut(s)	1923
RgaI	GCGATCGC	1 cut(s)	1687
RsaI	GTAC	6 cut(s)	734, 974, 1840, 1916, 2003, 2156
RsaNI	GTAC	6 cut(s)	733, 973, 1839, 1915, 2002, 2155
RseI	CAYNNNNRTG	1 cut(s)	1325
SacI	GAGCTC	1 cut(s)	2175
SalI	GTCGAC	1 cut(s)	869
SapI	GCTCTTC	1 cut(s)	1743
SaqAI	TTAA	4 cut(s)	20, 215, 885, 1473
SatI	GCNGC	9 cut(s)	6, 776, 822, 1253, 1256, 1259, 1262, 1299, 1924
Sau96I	GGNCC	6 cut(s)	392, 986, 1066, 1189, 1661, 2251
SchI	GAGTC	2 cut(s)	1421, 2073
ScrFI	CCNGG	3 cut(s)	482, 654, 2009
SduI	GDGCHC	2 cut(s)	471, 2175
SfaAI	GCGATCGC	1 cut(s)	1687
SfaNI	GCATC	4 cut(s)	191, 688, 751, 2211
SfcI	CTRYAG	3 cut(s)	1310, 1918, 2176
Sfr274I	CTCGAG	1 cut(s)	248
SgfI	GCGATCGC	1 cut(s)	1687
SinI	GGWCC	6 cut(s)	392, 986, 1066, 1189, 1661, 2251
SlaI	CTCGAG	1 cut(s)	248
SmiMI	CAYNNNNRTG	1 cut(s)	1325
SmlI	CTYRAG	1 cut(s)	248
SmoI	CTYRAG	1 cut(s)	248
SphI	GCATGC	2 cut(s)	944, 1124
Sse9I	AATT	8 cut(s)	297, 339, 485, 601, 1075, 1132, 1348, 2019
SsiI	CCGC	6 cut(s)	6, 775, 824, 1145, 2162, 2247
SspI	AATATT	1 cut(s)	499
SspMI	CTAG	5 cut(s)	269, 453, 818, 1265, 1754
SstI	GAGCTC	1 cut(s)	2175
StyD4I	CCNGG	3 cut(s)	480, 652, 2007
StyI	CCWWGG	4 cut(s)	144, 1137, 1246, 1983
TaiI	ACGT	4 cut(s)	546, 1964, 2034, 2045
TaqII	GACCGA	2 cut(s)	888, 2268
TasI	AATT	8 cut(s)	297, 339, 485, 601, 1075, 1132, 1348, 2019
TatI	WGTACW	5 cut(s)	732, 972, 1838, 1914, 2154
TauI	GCSGC	2 cut(s)	8, 778
Tru1I	TTAA	4 cut(s)	20, 215, 885, 1473
Tru9I	TTAA	4 cut(s)	20, 215, 885, 1473
TseFI	GTSAC	2 cut(s)	222, 443
TseI	GCWGC	7 cut(s)	821, 1252, 1255, 1258, 1261, 1298, 1923
Tsp45I	GTSAC	2 cut(s)	222, 443
TspDTI	ATGAA	3 cut(s)	824, 1043, 1543
TspGWI	ACGGA	4 cut(s)	1525, 1933, 2126, 2240
VpaK11BI	GGWCC	6 cut(s)	392, 986, 1066, 1189, 1661, 2251
XapI	RAATTY	3 cut(s)	339, 485, 1132
XceI	RCATGY	3 cut(s)	944, 954, 1124
XhoI	CTCGAG	1 cut(s)	248
XmiI	GTMKAC	1 cut(s)	870
XmnI	GAANNNNTTC	1 cut(s)	2281
XspI	CTAG	5 cut(s)	269, 453, 818, 1265, 1754
ZraI	GACGTC	1 cut(s)	2043

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.