RLG00000008227

Selenium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014197
Physical Location & Seq
Reverse (-)
27409874 .. 27413283
3410 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008227

Sequence Viewer

Length: 1464 bp
ATGGCTACGGATGTGGTAGAAGGGAAGAGGCAGGGCAATGGCGACGCAGCATCATGCTGCAAGACTGGGCCGGGTTACGCCACCCCACTCCAGGCCATGTCTGGTCCCAGAGAGCAAATCCTCTATGTCACTGCTGTTTACTCTGGAACCGGGATTCAGAAGCCTGATTACCTGGCCACAGTGGACGTGGATCCAAATTCTCCAACTTATTCCAAAGTTATCCACAGGCTGCCTGTGCCGAATTTAGGCGATGAACTGCATCACTCTGGGTGGAACTCTTGCAGCTCTTGCCATGGAGATCCATCTAAGCAGCGGCGTTTCCTTGTCCTTCCTGCATTGGTATCTAGTCGCATATATGTGATCGACACAAAAACAGATCCAAAAGCTCCCTCTCTGCATAAAGTTGTTGACCCTGAAGACATCGTGCAGAAGACTGGATTGGCATACCCACACACATCTCACTGCCTTGCATCTGGTGATTTGTTGGTTTCTTGCCTAGGGGATAAGGATGGAAATGCTGCAGGAAACGGATTTCTTCTCCTTGACTCAGAGTTTAATGTCAAGGGAAGGTGGGAGAAACCAGGGCACAGTCCGCTTTTTGGCTACGATTTCTGGTACCAACCACGGCACAAAACAATGATAAGCACATCATGGGGTGCCCCTGCTGCTTTCACCAAAGGTTTCAACCTTCAGCATGTCTTTGATGGTCTCTATGGGAGGCATCTTCATGTCTACAGCTGGCCTGAGGGTGAATTGAAACAAACTTTGGATCTTGGCAATACAGGGCTCTTACCCTTGGAGATAAGGTTCCTCCATGATCCTTCTAAGGACACTGGGTTTGTTGGGTGTGCCTTGTCAAGTAACGTGGTGCGATTCTTCAAGACAGAGGATGGTTCATGGAGCCACGAGGTTGTAATATCAGTAGAACCACTGAAGGTGCAGAATTGGATTCTTCCAGAGATGCCTGGGCTCATAACTGATTTTTTAATCTCACTTGATGATCGTTTTCTATACTTTGTCAACTGGCTTCATGGAGATGTAAGACAATATAACATTGAGGACCCTAAAAATCCTAAACTGACTGGCCAAGTATGGGTTGGGGGACTTATCCAAAAGGGAAGCCCAGTTGTCGTTGAGGGTGAAGATGGTACAACAAAGCAGTTTGATGTTCCGGAGATCCAGGGGAAAAAATTGAGAGGTGGACCACAAATGATCCAGTTGAGTTTAGATGGGAAGCGGCTTTATGTCACCAACTCGCTTTTCAGTACATGGGATCGCCAGTTCTACCCTGACCTTCTGGAGAAAGGATCCCACATGTTACAACTTGATGTTGATACTGAGAAAGGTGGTCTTGCATTAAACACAAAGTTTTTCGTCGACTTTGGAGCCGAACCTGATGGTCCTTCTCTTGCCCATGAAATGAGATACCCTGGTGGTGACTGCACGTCTGATATATGGATTTGA

Protein Analysis

488

Amino Acids

53.89

Weight (kDa)

5.7

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP56 PF05694 20 - 487 1.2e-227 56kDa selenium binding protein (SBP56)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23800 AT3G23800 AT4G14030 AT4G14030 AT4G14040
fragaria_vesca FvH4_4g13170
malus_domestica MD04G1018500.v1.1
prunus_persica Prupe.1G167300_v2.0.a1 Prupe.1G167300_v2.0.a1
pyrus_communis pycom04g01480
rosa_chinensis RchiOBHm_Chr4g0413261
rosa_laevigata RLG00000008227
rosa_multiflora Rmu_sc0000717.1_g000003
rosa_roxburghii Rroxscaffold_5G00357230
rosa_rugosa Rorug04G0119000
rosa_samantha Rh4AG178900
rosa_wichuraiana Rw4G014880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 615
AccB1I GGYRCC 2 cut(s) 615, 656
AccI GTMKAC 2 cut(s) 732, 1377
AccIII TCCGGA 1 cut(s) 1171
AciI CCGC 3 cut(s) 313, 593, 1237
AcoI YGGCCR 2 cut(s) 174, 1084
AcsI RAATTY 2 cut(s) 196, 241
AcuI CTGAAG 3 cut(s) 435, 674, 953
AfaI GTAC 3 cut(s) 617, 1150, 1267
AfiI CCNNNNNNNGG 4 cut(s) 91, 245, 599, 1093
AflIII ACRYGT 1 cut(s) 1314
AgsI TTSAA 3 cut(s) 685, 757, 880
AhdI GACNNNNNGTC 1 cut(s) 1444
AjiI CACGTC 2 cut(s) 187, 1446
AjnI CCWGG 6 cut(s) 90, 171, 580, 964, 1179, 1429
AjuI GAANNNNNNNTTGG 6 cut(s) 422, 454, 749, 781, 1244, 1276
AloI GAACNNNNNNTCC 4 cut(s) 791, 823, 1265, 1297
AluBI AGCT 3 cut(s) 285, 386, 738
AluI AGCT 3 cut(s) 285, 386, 738
Alw26I GTCTC 1 cut(s) 713
Aor13HI TCCGGA 1 cut(s) 1171
AoxI GGCC 5 cut(s) 68, 93, 174, 740, 1084
ApeKI GCWGC 7 cut(s) 47, 57, 229, 282, 310, 518, 665
ApoI RAATTY 2 cut(s) 196, 241
ArsI GACNNNNNNTTYG 2 cut(s) 821, 853
Asp718I GGTACC 1 cut(s) 615
AspA2I CCTAGG 1 cut(s) 496
AspS9I GGNCC 5 cut(s) 68, 104, 1060, 1202, 1400
AsuC2I CCSGG 2 cut(s) 72, 151
AsuHPI GGTGA 6 cut(s) 488, 664, 761, 1151, 1240, 1448
AvaII GGWCC 4 cut(s) 104, 1060, 1202, 1400
AvrII CCTAGG 1 cut(s) 496
AxyI CCTNAGG 1 cut(s) 744
BaeGI GKGCMC 2 cut(s) 588, 661
BaeI ACNNNNGTAYC 2 cut(s) 1417, 1450
BalI TGGCCA 2 cut(s) 176, 1086
BamHI GGATCC 2 cut(s) 190, 1307
BanI GGYRCC 2 cut(s) 615, 656
BanII GRGCYC 2 cut(s) 789, 972
BarI GAAGNNNNNNTAC 2 cut(s) 152, 184
BauI CACGAG 1 cut(s) 905
BbsI GAAGAC 2 cut(s) 423, 437
BbvI GCAGC 7 cut(s) 44, 59, 216, 294, 322, 505, 652
BccI CCATC 7 cut(s) 310, 503, 698, 884, 1139, 1223, 1391
BceAI ACGGC 1 cut(s) 641
BciT130I CCWGG 6 cut(s) 92, 173, 582, 966, 1181, 1431
BcnI CCSGG 2 cut(s) 72, 151
BcoDI GTCTC 1 cut(s) 713
BfaI CTAG 2 cut(s) 345, 497
BfmI CTRYAG 2 cut(s) 519, 733
BisI GCNGC 9 cut(s) 48, 58, 230, 283, 311, 314, 519, 666, 1238
BlnI CCTAGG 1 cut(s) 496
BlsI GCNGC 9 cut(s) 49, 59, 231, 284, 312, 315, 520, 667, 1239
Bme1390I CCNGG 8 cut(s) 72, 92, 151, 173, 582, 966, 1181, 1431
Bme18I GGWCC 4 cut(s) 104, 1060, 1202, 1400
BmeRI GACNNNNNGTC 1 cut(s) 1444
BmgBI CACGTC 2 cut(s) 187, 1446
BmgT120I GGNCC 5 cut(s) 68, 104, 1060, 1202, 1400
BmrFI CCNGG 8 cut(s) 72, 92, 151, 173, 582, 966, 1181, 1431
BmrI ACTGGG 3 cut(s) 75, 843, 1118
BmsI GCATC 5 cut(s) 59, 268, 479, 730, 951
BmuI ACTGGG 3 cut(s) 75, 843, 1118
BpiI GAAGAC 2 cut(s) 423, 437
BpmI CTGGAG 2 cut(s) 74, 1319
BpuMI CCSGG 2 cut(s) 72, 151
BsaI GGTCTC 1 cut(s) 713
BsaJI CCNNGG 8 cut(s) 292, 496, 581, 623, 795, 965, 1180, 1429
BsaWI WCCGGW 1 cut(s) 1171
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 245, 599, 1093
Bse1I ACTGG 8 cut(s) 70, 439, 838, 1028, 1087, 1124, 1216, 1279
Bse21I CCTNAGG 1 cut(s) 744
Bse3DI GCAATG 1 cut(s) 43
BseAI TCCGGA 1 cut(s) 1171
BseBI CCWGG 6 cut(s) 92, 173, 582, 966, 1181, 1431
BseDI CCNNGG 8 cut(s) 292, 496, 581, 623, 795, 965, 1180, 1429
BseGI GGATG 3 cut(s) 16, 514, 895
BseLI CCNNNNNNNGG 4 cut(s) 91, 245, 599, 1093
BseMI GCAATG 1 cut(s) 43
BseMII CTCAG 3 cut(s) 561, 735, 1329
BseNI ACTGG 8 cut(s) 70, 439, 838, 1028, 1087, 1124, 1216, 1279
BseSI GKGCMC 2 cut(s) 588, 661
BseXI GCAGC 7 cut(s) 44, 59, 216, 294, 322, 505, 652
BsgI GTGCAG 3 cut(s) 446, 959, 1426
BshFI GGCC 5 cut(s) 70, 95, 176, 742, 1086
BshNI GGYRCC 2 cut(s) 615, 656
BsiSI CCGG 3 cut(s) 71, 150, 1172
BslFI GGGAC 2 cut(s) 90, 1116
BslI CCNNNNNNNGG 4 cut(s) 91, 245, 599, 1093
BsmAI GTCTC 1 cut(s) 713
BsmFI GGGAC 2 cut(s) 90, 1116
BsnI GGCC 5 cut(s) 70, 95, 176, 742, 1086
Bso31I GGTCTC 1 cut(s) 713
Bsp1286I GDGCHC 4 cut(s) 588, 661, 789, 972
Bsp13I TCCGGA 1 cut(s) 1171
Bsp19I CCATGG 1 cut(s) 292
BspACI CCGC 3 cut(s) 313, 593, 1237
BspANI GGCC 5 cut(s) 70, 95, 176, 742, 1086
BspCNI CTCAG 3 cut(s) 560, 736, 1330
BspEI TCCGGA 1 cut(s) 1171
BspMAI CTGCAG 1 cut(s) 523
BspT107I GGYRCC 2 cut(s) 615, 656
BspTNI GGTCTC 1 cut(s) 713
BsrDI GCAATG 1 cut(s) 43
BsrI ACTGG 8 cut(s) 70, 439, 838, 1028, 1087, 1124, 1216, 1279
BssECI CCNNGG 8 cut(s) 292, 496, 581, 623, 795, 965, 1180, 1429
BssSI CACGAG 1 cut(s) 905
BssT1I CCWWGG 3 cut(s) 292, 496, 795
Bst2BI CACGAG 1 cut(s) 905
Bst2UI CCWGG 6 cut(s) 92, 173, 582, 966, 1181, 1431
Bst4CI ACNGT 2 cut(s) 181, 590
Bst6I CTCTTC 1 cut(s) 20
BstAPI GCANNNNNTGC 1 cut(s) 288
BstC8I GCNNGC 1 cut(s) 740
BstDEI CTNAG 5 cut(s) 306, 547, 744, 825, 1338
BstDSI CCRYGG 2 cut(s) 292, 623
BstF5I GGATG 3 cut(s) 16, 514, 895
BstMAI GTCTC 1 cut(s) 713
BstMWI GCNNNNNNNGC 4 cut(s) 235, 288, 592, 665
BstNI CCWGG 6 cut(s) 92, 173, 582, 966, 1181, 1431
BstNSI RCATGY 2 cut(s) 698, 1318
BstSCI CCNGG 8 cut(s) 70, 90, 149, 171, 580, 964, 1179, 1429
BstSFI CTRYAG 2 cut(s) 519, 733
BstSLI GKGCMC 2 cut(s) 588, 661
BstV1I GCAGC 7 cut(s) 44, 59, 216, 294, 322, 505, 652
BstV2I GAAGAC 2 cut(s) 423, 437
BstX2I RGATCY 6 cut(s) 190, 298, 376, 769, 1176, 1307
BstYI RGATCY 6 cut(s) 190, 298, 376, 769, 1176, 1307
Bsu36I CCTNAGG 1 cut(s) 744
BsuRI GGCC 5 cut(s) 70, 95, 176, 742, 1086
BtgI CCRYGG 2 cut(s) 292, 623
BtgZI GCGATG 1 cut(s) 264
BtrI CACGTC 2 cut(s) 187, 1446
BtsCI GGATG 3 cut(s) 16, 514, 895
BtsI GCAGTG 2 cut(s) 129, 460
BtsIMutI CAGTG 5 cut(s) 129, 186, 460, 831, 929
Cac8I GCNNGC 1 cut(s) 740
Cfr13I GGNCC 5 cut(s) 68, 104, 1060, 1202, 1400
CseI GACGC 1 cut(s) 53
Csp6I GTAC 3 cut(s) 616, 1149, 1266
CspCI CAANNNNNGTGG 2 cut(s) 846, 881
CviQI GTAC 3 cut(s) 616, 1149, 1266
DdeI CTNAG 5 cut(s) 306, 547, 744, 825, 1338
DriI GACNNNNNGTC 1 cut(s) 1444
EaeI YGGCCR 2 cut(s) 174, 1084
Eam1104I CTCTTC 1 cut(s) 20
Eam1105I GACNNNNNGTC 1 cut(s) 1444
EarI CTCTTC 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 292, 496, 795
Eco24I GRGCYC 2 cut(s) 789, 972
Eco31I GGTCTC 1 cut(s) 713
Eco47I GGWCC 4 cut(s) 104, 1060, 1202, 1400
Eco57I CTGAAG 3 cut(s) 435, 674, 953
Eco81I CCTNAGG 1 cut(s) 744
EcoO109I RGGNCCY 1 cut(s) 1060
EcoRII CCWGG 6 cut(s) 90, 171, 580, 964, 1179, 1429
EcoT14I CCWWGG 3 cut(s) 292, 496, 795
EcoT38I GRGCYC 2 cut(s) 789, 972
ErhI CCWWGG 3 cut(s) 292, 496, 795
FalI AAGNNNNNCTT 2 cut(s) 1335, 1367
FaqI GGGAC 2 cut(s) 90, 1116
FblI GTMKAC 2 cut(s) 732, 1377
Fnu4HI GCNGC 9 cut(s) 48, 58, 230, 283, 311, 314, 519, 666, 1238
FokI GGATG 3 cut(s) 23, 521, 902
FriOI GRGCYC 2 cut(s) 789, 972
Fsp4HI GCNGC 9 cut(s) 48, 58, 230, 283, 311, 314, 519, 666, 1238
FspBI CTAG 2 cut(s) 345, 497
GluI GCNGC 9 cut(s) 48, 58, 230, 283, 311, 314, 519, 666, 1238
GsuI CTGGAG 2 cut(s) 74, 1319
HaeIII GGCC 5 cut(s) 70, 95, 176, 742, 1086
HapII CCGG 3 cut(s) 71, 150, 1172
HgaI GACGC 1 cut(s) 53
HincII GTYRAC 3 cut(s) 409, 1021, 1378
HindII GTYRAC 3 cut(s) 409, 1021, 1378
HinfI GANTC 4 cut(s) 154, 545, 873, 949
HpaII CCGG 3 cut(s) 71, 150, 1172
HphI GGTGA 6 cut(s) 488, 664, 761, 1151, 1240, 1448
Hpy166II GTNNAC 7 cut(s) 139, 184, 409, 733, 1021, 1202, 1378
Hpy188I TCNGA 3 cut(s) 159, 550, 1450
Hpy188III TCNNGA 5 cut(s) 144, 880, 956, 1172, 1298
Hpy8I GTNNAC 7 cut(s) 139, 184, 409, 733, 1021, 1202, 1378
Hpy99I CGWCG 2 cut(s) 47, 1379
HpyAV CCTTC 8 cut(s) 14, 338, 561, 698, 831, 928, 1304, 1413
HpyCH4III ACNGT 2 cut(s) 181, 590
HpyCH4IV ACGT 3 cut(s) 186, 864, 1445
HpyF10VI GCNNNNNNNGC 4 cut(s) 235, 288, 592, 665
HpyF3I CTNAG 5 cut(s) 306, 547, 744, 825, 1338
HpySE526I ACGT 3 cut(s) 186, 864, 1445
Kpn2I TCCGGA 1 cut(s) 1171
KpnI GGTACC 1 cut(s) 619
LmnI GCTCC 3 cut(s) 391, 900, 1385
Lsp1109I GCAGC 7 cut(s) 44, 59, 216, 294, 322, 505, 652
LweI GCATC 5 cut(s) 59, 268, 479, 730, 951
MaeI CTAG 2 cut(s) 345, 497
MaeII ACGT 3 cut(s) 186, 864, 1445
MaeIII GTNAC 6 cut(s) 74, 127, 860, 1246, 1317, 1436
MboII GAAGA 8 cut(s) 37, 428, 442, 527, 716, 868, 944, 1154
MflI RGATCY 6 cut(s) 190, 298, 376, 769, 1176, 1307
MhlI GDGCHC 4 cut(s) 588, 661, 789, 972
MlsI TGGCCA 2 cut(s) 176, 1086
MluCI AATT 5 cut(s) 196, 241, 752, 943, 1190
MluNI TGGCCA 2 cut(s) 176, 1086
MlyI GAGTC 1 cut(s) 539
MmeI TCCRAC 1 cut(s) 227
Mox20I TGGCCA 2 cut(s) 176, 1086
MroI TCCGGA 1 cut(s) 1171
MscI TGGCCA 2 cut(s) 176, 1086
MseI TTAA 3 cut(s) 555, 986, 1358
MslI CAYNNNNRTG 3 cut(s) 356, 726, 1035
Msp20I TGGCCA 2 cut(s) 176, 1086
MspA1I CMGCKG 2 cut(s) 313, 738
MspI CCGG 3 cut(s) 71, 150, 1172
MspR9I CCNGG 8 cut(s) 72, 92, 151, 173, 582, 966, 1181, 1431
MvaI CCWGG 6 cut(s) 92, 173, 582, 966, 1181, 1431
MwoI GCNNNNNNNGC 4 cut(s) 235, 288, 592, 665
NciI CCSGG 2 cut(s) 72, 151
NcoI CCATGG 1 cut(s) 292
NmuCI GTSAC 3 cut(s) 127, 1246, 1436
NspI RCATGY 2 cut(s) 698, 1318
PciI ACATGT 1 cut(s) 1314
PfeI GAWTC 3 cut(s) 154, 873, 949
PkrI GCNGC 9 cut(s) 49, 59, 231, 284, 312, 315, 520, 667, 1239
PleI GAGTC 1 cut(s) 539
PpsI GAGTC 1 cut(s) 539
PpuMI RGGWCCY 1 cut(s) 1060
PscI ACATGT 1 cut(s) 1314
Psp5II RGGWCCY 1 cut(s) 1060
Psp6I CCWGG 6 cut(s) 90, 171, 580, 964, 1179, 1429
PspGI CCWGG 6 cut(s) 90, 171, 580, 964, 1179, 1429
PspPI GGNCC 5 cut(s) 68, 104, 1060, 1202, 1400
PspPPI RGGWCCY 1 cut(s) 1060
PstI CTGCAG 1 cut(s) 523
PsuI RGATCY 6 cut(s) 190, 298, 376, 769, 1176, 1307
PvuII CAGCTG 1 cut(s) 738
RsaI GTAC 3 cut(s) 617, 1150, 1267
RsaNI GTAC 3 cut(s) 616, 1149, 1266
RseI CAYNNNNRTG 3 cut(s) 356, 726, 1035
SalI GTCGAC 1 cut(s) 1376
SaqAI TTAA 3 cut(s) 555, 986, 1358
SatI GCNGC 9 cut(s) 48, 58, 230, 283, 311, 314, 519, 666, 1238
Sau96I GGNCC 5 cut(s) 68, 104, 1060, 1202, 1400
SchI GAGTC 1 cut(s) 539
ScrFI CCNGG 8 cut(s) 72, 92, 151, 173, 582, 966, 1181, 1431
SduI GDGCHC 4 cut(s) 588, 661, 789, 972
SfaNI GCATC 5 cut(s) 59, 268, 479, 730, 951
SfcI CTRYAG 2 cut(s) 519, 733
SinI GGWCC 4 cut(s) 104, 1060, 1202, 1400
SmiMI CAYNNNNRTG 3 cut(s) 356, 726, 1035
Sse9I AATT 5 cut(s) 196, 241, 752, 943, 1190
SsiI CCGC 3 cut(s) 313, 593, 1237
SspMI CTAG 2 cut(s) 345, 497
StyD4I CCNGG 8 cut(s) 70, 90, 149, 171, 580, 964, 1179, 1429
StyI CCWWGG 3 cut(s) 292, 496, 795
TaaI ACNGT 2 cut(s) 181, 590
TaiI ACGT 3 cut(s) 189, 867, 1448
TaqI TCGA 2 cut(s) 363, 1377
TasI AATT 5 cut(s) 196, 241, 752, 943, 1190
TatI WGTACW 1 cut(s) 1265
TauI GCSGC 2 cut(s) 316, 1240
TfiI GAWTC 3 cut(s) 154, 873, 949
Tru1I TTAA 3 cut(s) 555, 986, 1358
Tru9I TTAA 3 cut(s) 555, 986, 1358
TscAI CASTG 5 cut(s) 136, 186, 467, 838, 936
TseFI GTSAC 3 cut(s) 127, 1246, 1436
TseI GCWGC 7 cut(s) 47, 57, 229, 282, 310, 518, 665
Tsp45I GTSAC 3 cut(s) 127, 1246, 1436
TspDTI ATGAA 5 cut(s) 267, 716, 885, 1019, 1431
TspGWI ACGGA 2 cut(s) 23, 543
TspRI CASTG 5 cut(s) 136, 186, 467, 838, 936
VpaK11BI GGWCC 4 cut(s) 104, 1060, 1202, 1400
XapI RAATTY 2 cut(s) 196, 241
XceI RCATGY 2 cut(s) 698, 1318
XcmI CCANNNNNNNNNTGG 3 cut(s) 98, 184, 1094
XmaJI CCTAGG 1 cut(s) 496
XmiI GTMKAC 2 cut(s) 732, 1377
XspI CTAG 2 cut(s) 345, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.