RLG00000008795

Phospholipase A-2-activating

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0030686
Physical Location & Seq
Forward (+)
39328416 .. 39328950
535 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008795

Sequence Viewer

Length: 336 bp
ATGGCCATGCATATTGATTTTAGAGACTACCAATTGCATTGCAAGCTCCGCGGCCACGAAGACGACGTTATGGTCCTTGACCCTTCCGACGAACGCCGGTATGCGCCCGCGAAGATTCTGCTGGGCCACATGAGTTTTGTGGGCCCAGTGGTGTGGATTCAGCCCAATGAGGAGTATCCAGAAGGCCGAATTGTGTCTGGTGGCATGGACACTATGGTATTGGTGTGGGACTTGAAGACTGGGGAGAAGGTCCAAGCTCTCAAGGGACATCAGTTGCAGGTCACTGGCCTCGCTTTCGATAATGGAGACATTGTTTCATCTTCTGTGGACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001558 GO:0002237 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006082 GO:0006508 GO:0006511 GO:0006629 GO:0006631 GO:0006690 GO:0006692 GO:0006693 GO:0006807 GO:0006914 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009892 GO:0009987 GO:0010033 GO:0010517 GO:0010518 GO:0010605 GO:0010720 GO:0010975 GO:0010976 GO:0016236 GO:0019222 GO:0019538 GO:0019752 GO:0019941 GO:0022008 GO:0022603 GO:0022604 GO:0030154 GO:0030163 GO:0030307 GO:0030334 GO:0030335 GO:0031323 GO:0031324 GO:0031344 GO:0031346 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032429 GO:0032430 GO:0032496 GO:0032501 GO:0032502 GO:0032787 GO:0032879 GO:0033559 GO:0033993 GO:0040008 GO:0040012 GO:0040017 GO:0042221 GO:0043085 GO:0043162 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044424 GO:0044464 GO:0045202 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045927 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0051960 GO:0051962 GO:0060191 GO:0060193 GO:0060255 GO:0060284 GO:0060341 GO:0061919 GO:0065007 GO:0065009 GO:0070887 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071396 GO:0071704 GO:0080090 GO:0098693 GO:0120035 GO:1900044 GO:1900045 GO:1901564 GO:1901565 GO:1901568 GO:1901575 GO:1901700 GO:1901701 GO:1902914 GO:1902915 GO:1903320 GO:1903321 GO:1903421 GO:1903423 GO:1903859 GO:1903861 GO:2000026 GO:2000145 GO:2000147 GO:2001222 GO:2001224
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PFU PUL WD40

Protein Analysis

112

Amino Acids

12.48

Weight (kDa)

5.19

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 28 - 103 2.3e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR41 PF25178 33 - 98 6.2e-06 WDR41
Beta-prop_WDR5 PF25175 36 - 110 1e-09 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 61 - 111 2.6e-09 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 62 - 111 9.1e-07 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0030686)

Species Orthologous Gene IDs
rosa_laevigata RLG00000008795
rosa_multiflora Rmu_sc0007280.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
Acc36I ACCTGC 1 cut(s) 268
AccII CGCG 2 cut(s) 51, 110
AciI CCGC 3 cut(s) 49, 51, 108
AcoI YGGCCR 2 cut(s) 3, 52
AgsI TTSAA 1 cut(s) 235
AluBI AGCT 2 cut(s) 46, 257
AluI AGCT 2 cut(s) 46, 257
Alw26I GTCTC 2 cut(s) 18, 300
AoxI GGCC 6 cut(s) 3, 52, 124, 142, 184, 286
ApaI GGGCCC 1 cut(s) 146
AspLEI GCGC 1 cut(s) 106
AspS9I GGNCC 5 cut(s) 73, 124, 142, 143, 250
AvaII GGWCC 2 cut(s) 73, 250
BaeGI GKGCMC 1 cut(s) 146
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 146
BbsI GAAGAC 2 cut(s) 66, 242
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciVI GTATCC 1 cut(s) 186
BcoDI GTCTC 2 cut(s) 18, 300
BfuAI ACCTGC 1 cut(s) 268
BfuI GTATCC 1 cut(s) 186
BisI GCNGC 1 cut(s) 52
BlsI GCNGC 1 cut(s) 53
Bme18I GGWCC 2 cut(s) 73, 250
BmgT120I GGNCC 5 cut(s) 73, 124, 142, 143, 250
BmiI GGNNCC 1 cut(s) 144
BmrI ACTGGG 2 cut(s) 140, 249
BmuI ACTGGG 2 cut(s) 140, 249
BpiI GAAGAC 2 cut(s) 66, 242
BpuEI CTTGAG 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 49
BsaXI ACNNNNNCTCC 2 cut(s) 297, 327
Bse118I RCCGGY 1 cut(s) 96
Bse1I ACTGG 3 cut(s) 146, 244, 289
Bse3DI GCAATG 1 cut(s) 37
BseDI CCNNGG 1 cut(s) 49
BseMI GCAATG 1 cut(s) 37
BseNI ACTGG 3 cut(s) 146, 244, 289
BseRI GAGGAG 1 cut(s) 185
BseSI GKGCMC 1 cut(s) 146
BseYI CCCAGC 1 cut(s) 121
Bsh1236I CGCG 2 cut(s) 51, 110
BshFI GGCC 6 cut(s) 5, 54, 126, 144, 186, 288
BsiSI CCGG 1 cut(s) 97
BslFI GGGAC 2 cut(s) 242, 279
BsmAI GTCTC 2 cut(s) 18, 300
BsmFI GGGAC 2 cut(s) 242, 279
BsnI GGCC 6 cut(s) 5, 54, 126, 144, 186, 288
Bsp120I GGGCCC 1 cut(s) 142
Bsp1286I GDGCHC 1 cut(s) 146
BspACI CCGC 3 cut(s) 49, 51, 108
BspANI GGCC 6 cut(s) 5, 54, 126, 144, 186, 288
BspFNI CGCG 2 cut(s) 51, 110
BspLI GGNNCC 1 cut(s) 144
BspMI ACCTGC 1 cut(s) 268
BsrDI GCAATG 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 96
BsrI ACTGG 3 cut(s) 146, 244, 289
BssAI RCCGGY 1 cut(s) 96
BssECI CCNNGG 1 cut(s) 49
BstC8I GCNNGC 2 cut(s) 44, 108
BstDSI CCRYGG 1 cut(s) 49
BstFNI CGCG 2 cut(s) 51, 110
BstHHI GCGC 1 cut(s) 106
BstMAI GTCTC 2 cut(s) 18, 300
BstMWI GCNNNNNNNGC 2 cut(s) 43, 48
BstSLI GKGCMC 1 cut(s) 146
BstUI CGCG 2 cut(s) 51, 110
BstV2I GAAGAC 2 cut(s) 66, 242
BstXI CCANNNNNNTGG 1 cut(s) 153
BsuI GTATCC 1 cut(s) 186
BsuRI GGCC 6 cut(s) 5, 54, 126, 144, 186, 288
BtgI CCRYGG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 153, 282
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 2 cut(s) 44, 108
CfoI GCGC 1 cut(s) 106
Cfr10I RCCGGY 1 cut(s) 96
Cfr13I GGNCC 5 cut(s) 73, 124, 142, 143, 250
Cfr42I CCGCGG 1 cut(s) 52
CviAII CATG 3 cut(s) 7, 130, 205
CviJI RGCY 9 cut(s) 5, 46, 54, 126, 144, 163, 186, 257, 288
CviKI_1 RGCY 9 cut(s) 5, 46, 54, 126, 144, 163, 186, 257, 288
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 2 cut(s) 3, 52
Eco24I GRGCYC 1 cut(s) 146
Eco47I GGWCC 2 cut(s) 73, 250
EcoT22I ATGCAT 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 146
FaeI CATG 3 cut(s) 10, 133, 208
FaiI YATR 7 cut(s) 8, 12, 71, 102, 131, 206, 215
FaqI GGGAC 2 cut(s) 242, 279
FatI CATG 3 cut(s) 6, 129, 204
FauI CCCGC 1 cut(s) 115
Fnu4HI GCNGC 1 cut(s) 52
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 1 cut(s) 52
GlaI GCGC 1 cut(s) 105
GluI GCNGC 1 cut(s) 52
GsaI CCCAGC 1 cut(s) 125
HaeIII GGCC 6 cut(s) 5, 54, 126, 144, 186, 288
HapII CCGG 1 cut(s) 97
HhaI GCGC 1 cut(s) 106
Hin1II CATG 3 cut(s) 10, 133, 208
Hin6I GCGC 1 cut(s) 104
HinP1I GCGC 1 cut(s) 104
HinfI GANTC 2 cut(s) 115, 157
HpaII CCGG 1 cut(s) 97
Hpy166II GTNNAC 1 cut(s) 328
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 1 cut(s) 179
Hpy8I GTNNAC 1 cut(s) 328
Hpy99I CGWCG 2 cut(s) 68, 92
HpyAV CCTTC 3 cut(s) 93, 176, 241
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 4 cut(s) 10, 37, 42, 277
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 48
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 3 cut(s) 10, 133, 208
HspAI GCGC 1 cut(s) 104
KspI CCGCGG 1 cut(s) 52
LmnI GCTCC 1 cut(s) 51
LpnPI CCDG 8 cut(s) 107, 110, 159, 183, 192, 225, 263, 270
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 1 cut(s) 280
MboII GAAGA 4 cut(s) 71, 124, 247, 312
MfeI CAATTG 1 cut(s) 32
MhlI GDGCHC 1 cut(s) 146
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 32, 189
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 2 cut(s) 163, 299
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 12
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 1 cut(s) 97
MunI CAATTG 1 cut(s) 32
MvnI CGCG 2 cut(s) 51, 110
MwoI GCNNNNNNNGC 2 cut(s) 43, 48
NlaIII CATG 3 cut(s) 10, 133, 208
NlaIV GGNNCC 1 cut(s) 144
NmuCI GTSAC 1 cut(s) 280
NsiI ATGCAT 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PfeI GAWTC 2 cut(s) 115, 157
PkrI GCNGC 1 cut(s) 53
PspFI CCCAGC 1 cut(s) 121
PspN4I GGNNCC 1 cut(s) 144
PspOMI GGGCCC 1 cut(s) 142
PspPI GGNCC 5 cut(s) 73, 124, 142, 143, 250
SacII CCGCGG 1 cut(s) 52
SatI GCNGC 1 cut(s) 52
Sau96I GGNCC 5 cut(s) 73, 124, 142, 143, 250
SduI GDGCHC 1 cut(s) 146
SetI ASST 5 cut(s) 48, 69, 252, 259, 282
Sfr303I CCGCGG 1 cut(s) 52
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 2 cut(s) 73, 250
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 2 cut(s) 32, 189
SsiI CCGC 3 cut(s) 49, 51, 108
TaiI ACGT 1 cut(s) 69
TaqI TCGA 1 cut(s) 297
TasI AATT 2 cut(s) 32, 189
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 2 cut(s) 115, 157
TscAI CASTG 2 cut(s) 153, 289
TseFI GTSAC 1 cut(s) 280
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 1 cut(s) 306
TspRI CASTG 2 cut(s) 153, 289
VpaK11BI GGWCC 2 cut(s) 73, 250
Zsp2I ATGCAT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.