RLG00000009294

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0030641
Physical Location & Seq
Reverse (-)
50364836 .. 50369192
4357 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009294

Sequence Viewer

Length: 615 bp
ATGTTTTGGGGAATCCCACTGTGGAGTTTGGAGTACATGGGATCCAGGCCAGGTTCAGTCGATGGGGAGACTGGTGTTGTGGTGGGTCGGGCGCGAGCTGTCGGGCTTTGGTTTGCTGCAGACAATGTTGCAGATCCTGATGGCGGCCGCCTTGATCCAGAGTCTTCCCCCGCTCTGGCGTGCATTTCCTCGCAATCTGAGACGGGGCTCGCTTCGCTTTACTCACCTGGAGCGAGCCGTCCAAAAGCTCGATATTCTCGCCCTCTGTTGATTTTGCTGCTGCTTCTCCGAAACGTTTCAAGCATTCTGCCCCTGCCGCACCAGAAGCTTGTTCGGATTGGAGGCTGCTGCGGCGGCGGTGAGATCGACTGGGCTGTAGAGGCCCAGCTCGGTGTCGAAGACCGGCTTCTTGGCCGGGTCAGAGAGAACGGCCCATGCATCGGCGACGAGCTTGAAGGTGTGCTCGGCGAAGGAGTATTTGTTCTTGTCTGGTTGGAGGAGCAACACCAGCCGTCAATACGATCTCTTGATCAGATCGTGGTCGTCGGATCGGTGATCCACCTGGAGAATGGAGTACCAGTCGTGGTGGTTGTTTACGCACTTATCGGCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

21.67

Weight (kDa)

4.86

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030641)

Species Orthologous Gene IDs
rosa_laevigata RLG00000009294
rosa_multiflora Rmu_sc0005749.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 173
AccII CGCG 1 cut(s) 94
AciI CCGC 8 cut(s) 144, 148, 171, 317, 351, 354, 357, 609
AclI AACGTT 1 cut(s) 294
AclWI GGATC 6 cut(s) 36, 49, 128, 149, 550, 556
AcoI YGGCCR 2 cut(s) 145, 412
AfaI GTAC 2 cut(s) 35, 576
AfiI CCNNNNNNNGG 3 cut(s) 143, 175, 440
AgsI TTSAA 2 cut(s) 300, 455
AjnI CCWGG 4 cut(s) 44, 49, 226, 561
AloI GAACNNNNNNTCC 2 cut(s) 465, 497
AluBI AGCT 5 cut(s) 98, 248, 328, 388, 451
AluI AGCT 5 cut(s) 98, 248, 328, 388, 451
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 2 cut(s) 62, 194
AlwI GGATC 6 cut(s) 36, 49, 128, 149, 550, 556
AlwNI CAGNNNCTG 1 cut(s) 137
AoxI GGCC 5 cut(s) 47, 145, 381, 412, 430
ApeKI GCWGC 5 cut(s) 116, 277, 280, 345, 348
Asp700I GAANNNNTTC 1 cut(s) 295
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 2 cut(s) 382, 431
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 3 cut(s) 216, 371, 565
BamHI GGATCC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 210
BbsI GAAGAC 2 cut(s) 156, 405
Bbv12I GWGCWC 1 cut(s) 465
BbvI GCAGC 5 cut(s) 103, 264, 267, 332, 335
BccI CCATC 2 cut(s) 56, 134
BceAI ACGGC 3 cut(s) 222, 445, 496
BciT130I CCWGG 4 cut(s) 46, 51, 228, 563
BclI TGATCA 1 cut(s) 529
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 2 cut(s) 62, 194
BfmI CTRYAG 2 cut(s) 117, 375
Bme1390I CCNGG 5 cut(s) 46, 51, 228, 416, 563
BmgT120I GGNCC 2 cut(s) 382, 431
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 5 cut(s) 46, 51, 228, 416, 563
BmrI ACTGGG 1 cut(s) 379
BmsI GCATC 1 cut(s) 447
BmuI ACTGGG 1 cut(s) 379
BpiI GAAGAC 2 cut(s) 156, 405
BpmI CTGGAG 2 cut(s) 249, 584
BpuMI CCSGG 1 cut(s) 416
BsaXI ACNNNNNCTCC 4 cut(s) 465, 495, 564, 594
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 175, 440
Bse118I RCCGGY 1 cut(s) 402
Bse1I ACTGG 3 cut(s) 76, 374, 578
BseBI CCWGG 4 cut(s) 46, 51, 228, 563
BseLI CCNNNNNNNGG 3 cut(s) 143, 175, 440
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 3 cut(s) 76, 374, 578
BseRI GAGGAG 1 cut(s) 512
BseX3I CGGCCG 1 cut(s) 145
BseXI GCAGC 5 cut(s) 103, 264, 267, 332, 335
BseYI CCCAGC 1 cut(s) 384
Bsh1236I CGCG 1 cut(s) 94
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 5 cut(s) 49, 147, 383, 414, 432
BsiEI CGRYCG 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 465
BsiSI CCGG 2 cut(s) 403, 415
BslI CCNNNNNNNGG 3 cut(s) 143, 175, 440
BsmAI GTCTC 2 cut(s) 62, 194
BsmBI CGTCTC 1 cut(s) 194
BsmI GAATGC 1 cut(s) 303
BsnI GGCC 5 cut(s) 49, 147, 383, 414, 432
Bsp1286I GDGCHC 2 cut(s) 210, 465
Bsp143I GATC 9 cut(s) 41, 133, 154, 363, 521, 529, 534, 548, 555
BspACI CCGC 8 cut(s) 144, 148, 171, 317, 351, 354, 357, 609
BspANI GGCC 5 cut(s) 49, 147, 383, 414, 432
BspCNI CTCAG 1 cut(s) 190
BspFNI CGCG 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 43
BspMAI CTGCAG 1 cut(s) 121
BspPI GGATC 6 cut(s) 36, 49, 128, 149, 550, 556
BsrBI CCGCTC 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 402
BsrI ACTGG 3 cut(s) 76, 374, 578
BssAI RCCGGY 1 cut(s) 402
BssMI GATC 9 cut(s) 41, 133, 154, 363, 521, 529, 534, 548, 555
Bst2UI CCWGG 4 cut(s) 46, 51, 228, 563
Bst4CI ACNGT 1 cut(s) 21
BstC8I GCNNGC 4 cut(s) 96, 181, 210, 235
BstDEI CTNAG 1 cut(s) 198
BstFNI CGCG 1 cut(s) 94
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 9 cut(s) 44, 136, 157, 366, 524, 532, 537, 551, 558
BstMAI GTCTC 2 cut(s) 62, 194
BstMBI GATC 9 cut(s) 41, 133, 154, 363, 521, 529, 534, 548, 555
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 7 cut(s) 214, 316, 325, 351, 354, 380, 508
BstNI CCWGG 4 cut(s) 46, 51, 228, 563
BstSCI CCNGG 5 cut(s) 44, 49, 226, 414, 561
BstSFI CTRYAG 2 cut(s) 117, 375
BstUI CGCG 1 cut(s) 94
BstV1I GCAGC 5 cut(s) 103, 264, 267, 332, 335
BstV2I GAAGAC 2 cut(s) 156, 405
BstX2I RGATCY 2 cut(s) 41, 133
BstYI RGATCY 2 cut(s) 41, 133
BstZI CGGCCG 1 cut(s) 145
BsuRI GGCC 5 cut(s) 49, 147, 383, 414, 432
BtsIMutI CAGTG 1 cut(s) 17
Cac8I GCNNGC 4 cut(s) 96, 181, 210, 235
CaiI CAGNNNCTG 1 cut(s) 137
CciNI GCGGCCGC 1 cut(s) 145
CfoI GCGC 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 402
Cfr13I GGNCC 2 cut(s) 382, 431
Csp6I GTAC 2 cut(s) 34, 575
CviAII CATG 2 cut(s) 37, 435
CviQI GTAC 2 cut(s) 34, 575
DdeI CTNAG 1 cut(s) 198
DpnI GATC 9 cut(s) 43, 135, 156, 365, 523, 531, 536, 550, 557
DpnII GATC 9 cut(s) 41, 133, 154, 363, 521, 529, 534, 548, 555
EaeI YGGCCR 2 cut(s) 145, 412
EagI CGGCCG 1 cut(s) 145
EclXI CGGCCG 1 cut(s) 145
Eco24I GRGCYC 1 cut(s) 210
Eco52I CGGCCG 1 cut(s) 145
EcoRII CCWGG 4 cut(s) 44, 49, 226, 561
EcoT22I ATGCAT 1 cut(s) 440
EcoT38I GRGCYC 1 cut(s) 210
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 2 cut(s) 40, 438
FaiI YATR 2 cut(s) 38, 436
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FatI CATG 2 cut(s) 36, 434
FauI CCCGC 1 cut(s) 178
FbaI TGATCA 1 cut(s) 529
FriOI GRGCYC 1 cut(s) 210
GlaI GCGC 1 cut(s) 93
GsaI CCCAGC 1 cut(s) 388
GsuI CTGGAG 2 cut(s) 249, 584
HaeIII GGCC 5 cut(s) 49, 147, 383, 414, 432
HapII CCGG 2 cut(s) 403, 415
HhaI GCGC 1 cut(s) 94
Hin1II CATG 2 cut(s) 40, 438
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 2 cut(s) 12, 161
HpaII CCGG 2 cut(s) 403, 415
HphI GGTGA 3 cut(s) 216, 371, 565
Hpy166II GTNNAC 1 cut(s) 595
Hpy188I TCNGA 6 cut(s) 199, 290, 336, 422, 534, 548
Hpy188III TCNNGA 3 cut(s) 137, 158, 527
Hpy8I GTNNAC 1 cut(s) 595
Hpy99I CGWCG 2 cut(s) 449, 548
HpyAV CCTTC 2 cut(s) 449, 464
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 4 cut(s) 119, 131, 183, 438
HpyF10VI GCNNNNNNNGC 7 cut(s) 214, 316, 325, 351, 354, 380, 508
HpyF3I CTNAG 1 cut(s) 198
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 2 cut(s) 40, 438
HspAI GCGC 1 cut(s) 92
Ksp22I TGATCA 1 cut(s) 529
Kzo9I GATC 9 cut(s) 41, 133, 154, 363, 521, 529, 534, 548, 555
LmnI GCTCC 2 cut(s) 230, 499
Lsp1109I GCAGC 5 cut(s) 103, 264, 267, 332, 335
LweI GCATC 1 cut(s) 447
MaeII ACGT 1 cut(s) 294
MalI GATC 9 cut(s) 43, 135, 156, 365, 523, 531, 536, 550, 557
MbiI CCGCTC 1 cut(s) 173
MboI GATC 9 cut(s) 41, 133, 154, 363, 521, 529, 534, 548, 555
MboII GAAGA 2 cut(s) 156, 410
MflI RGATCY 2 cut(s) 41, 133
MhlI GDGCHC 2 cut(s) 210, 465
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 2 cut(s) 474, 526
MnlI CCTC 5 cut(s) 199, 273, 335, 373, 490
Mph1103I ATGCAT 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 295
MspI CCGG 2 cut(s) 403, 415
MspR9I CCNGG 5 cut(s) 46, 51, 228, 416, 563
Mva1269I GAATGC 1 cut(s) 303
MvaI CCWGG 4 cut(s) 46, 51, 228, 563
MvnI CGCG 1 cut(s) 94
MwoI GCNNNNNNNGC 7 cut(s) 214, 316, 325, 351, 354, 380, 508
NciI CCSGG 1 cut(s) 416
NdeII GATC 9 cut(s) 41, 133, 154, 363, 521, 529, 534, 548, 555
NlaIII CATG 2 cut(s) 40, 438
NlaIV GGNNCC 1 cut(s) 43
NmeAIII GCCGAG 1 cut(s) 444
NotI GCGGCCGC 1 cut(s) 145
NsiI ATGCAT 1 cut(s) 440
PctI GAATGC 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 295
PfeI GAWTC 1 cut(s) 12
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
Psp1406I AACGTT 1 cut(s) 294
Psp6I CCWGG 4 cut(s) 44, 49, 226, 561
PspFI CCCAGC 1 cut(s) 384
PspGI CCWGG 4 cut(s) 44, 49, 226, 561
PspN4I GGNNCC 1 cut(s) 43
PspPI GGNCC 2 cut(s) 382, 431
PstI CTGCAG 1 cut(s) 121
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 2 cut(s) 41, 133
RsaI GTAC 2 cut(s) 35, 576
RsaNI GTAC 2 cut(s) 34, 575
Sau3AI GATC 9 cut(s) 41, 133, 154, 363, 521, 529, 534, 548, 555
Sau96I GGNCC 2 cut(s) 382, 431
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 5 cut(s) 46, 51, 228, 416, 563
SduI GDGCHC 2 cut(s) 210, 465
SfaNI GCATC 1 cut(s) 447
SfcI CTRYAG 2 cut(s) 117, 375
SsiI CCGC 8 cut(s) 144, 148, 171, 317, 351, 354, 357, 609
StyD4I CCNGG 5 cut(s) 44, 49, 226, 414, 561
TaaI ACNGT 1 cut(s) 21
TaiI ACGT 1 cut(s) 297
TaqI TCGA 4 cut(s) 60, 250, 366, 396
TatI WGTACW 1 cut(s) 33
TauI GCSGC 5 cut(s) 147, 150, 319, 354, 357
TfiI GAWTC 1 cut(s) 12
TscAI CASTG 1 cut(s) 24
TseI GCWGC 5 cut(s) 116, 277, 280, 345, 348
TspRI CASTG 1 cut(s) 24
XcmI CCANNNNNNNNNTGG 1 cut(s) 566
XmnI GAANNNNTTC 1 cut(s) 295
Zsp2I ATGCAT 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.