RLG00000010837
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0025291
Physical Location & Seq
Forward (+)
4697624 .. 4698377
754 bp
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UTR
Exon/CDS
Intron
RLM00000010837

Sequence Viewer

Length: 306 bp
ATGGTCAAATGGAGAGCATTCCAACGAGCTACTCCACCCTCAAGAACACATGGCCACATGGAAGTGATGGAACCATATCTTCAGGCCTTCAGCTTCAGAAACTCCATGTCCCTCAAATCTGCAATTCAACTAGGCGGTACAGGAACAGTGGCCACGGCCATTGCTTATGCATTCCCACATATTGAATGCACTGTACTTGATCTCCGACATGTGGTGGCTGACCTGCAAGGAAGCAAGAACTTGAAATATACTGAAGTGACATGTTTGAGGCAGTTCCTCCTGCCGATGCAGTTTTACTTGAGGTAA

Protein Analysis

102

Amino Acids

11.52

Weight (kDa)

9.62

Isoelectric Point (pI)

58.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 36 - 75 1.7e-06 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0025291)

Species Orthologous Gene IDs
rosa_laevigata RLG00000010837
rosa_multiflora Rmu_sc0001809.1_g000060
rosa_rugosa Rorug06G0345800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 231
AciI CCGC 1 cut(s) 135
AcoI YGGCCR 3 cut(s) 52, 150, 156
AcuI CTGAAG 4 cut(s) 65, 73, 79, 273
AfaI GTAC 2 cut(s) 139, 195
AfiI CCNNNNNNNGG 1 cut(s) 211
AflIII ACRYGT 2 cut(s) 208, 260
AgsI TTSAA 3 cut(s) 128, 185, 244
AluBI AGCT 2 cut(s) 29, 93
AluI AGCT 2 cut(s) 29, 93
AoxI GGCC 4 cut(s) 52, 84, 150, 156
BalI TGGCCA 2 cut(s) 54, 152
BccI CCATC 1 cut(s) 61
BceAI ACGGC 1 cut(s) 171
BfaI CTAG 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 231
BmiI GGNNCC 1 cut(s) 72
BmsI GCATC 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 25
BsaJI CCNNGG 1 cut(s) 153
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse3DI GCAATG 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 153
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMI GCAATG 1 cut(s) 159
BshFI GGCC 4 cut(s) 54, 86, 152, 158
BslFI GGGAC 1 cut(s) 94
BslI CCNNNNNNNGG 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 94
BsmI GAATGC 3 cut(s) 17, 170, 191
BsnI GGCC 4 cut(s) 54, 86, 152, 158
Bsp143I GATC 1 cut(s) 199
BspACI CCGC 1 cut(s) 135
BspANI GGCC 4 cut(s) 54, 86, 152, 158
BspLI GGNNCC 1 cut(s) 72
BspMI ACCTGC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 159
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 1 cut(s) 199
Bst4CI ACNGT 2 cut(s) 148, 193
BstDSI CCRYGG 1 cut(s) 153
BstKTI GATC 1 cut(s) 202
BstMBI GATC 1 cut(s) 199
BstNSI RCATGY 2 cut(s) 212, 264
BsuRI GGCC 4 cut(s) 54, 86, 152, 158
BtgI CCRYGG 1 cut(s) 153
BtsIMutI CAGTG 2 cut(s) 153, 189
BveI ACCTGC 1 cut(s) 231
Csp6I GTAC 2 cut(s) 138, 194
CspCI CAANNNNNGTGG 2 cut(s) 142, 177
CviAII CATG 5 cut(s) 50, 58, 106, 209, 261
CviJI RGCY 7 cut(s) 29, 54, 86, 93, 152, 158, 218
CviKI_1 RGCY 7 cut(s) 29, 54, 86, 93, 152, 158, 218
CviQI GTAC 2 cut(s) 138, 194
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
EaeI YGGCCR 3 cut(s) 52, 150, 156
Eco147I AGGCCT 1 cut(s) 86
Eco57I CTGAAG 4 cut(s) 65, 73, 79, 273
EcoT22I ATGCAT 1 cut(s) 172
FaeI CATG 5 cut(s) 53, 61, 109, 212, 264
FaiI YATR 9 cut(s) 51, 59, 76, 107, 168, 180, 210, 249, 262
FaqI GGGAC 1 cut(s) 94
FatI CATG 5 cut(s) 49, 57, 105, 208, 260
FspBI CTAG 1 cut(s) 131
HaeIII GGCC 4 cut(s) 54, 86, 152, 158
Hin1II CATG 5 cut(s) 53, 61, 109, 212, 264
Hpy188I TCNGA 2 cut(s) 98, 206
Hpy188III TCNNGA 1 cut(s) 42
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 2 cut(s) 148, 193
HpyCH4V TGCA 5 cut(s) 122, 170, 189, 226, 289
Hsp92II CATG 5 cut(s) 53, 61, 109, 212, 264
Kzo9I GATC 1 cut(s) 199
LpnPI CCDG 4 cut(s) 68, 126, 236, 293
LweI GCATC 1 cut(s) 276
MaeI CTAG 1 cut(s) 131
MaeIII GTNAC 1 cut(s) 256
MalI GATC 1 cut(s) 201
MboI GATC 1 cut(s) 199
MboII GAAGA 1 cut(s) 71
MlsI TGGCCA 2 cut(s) 54, 152
MluCI AATT 1 cut(s) 123
MluNI TGGCCA 2 cut(s) 54, 152
MmeI TCCRAC 2 cut(s) 46, 229
MnlI CCTC 5 cut(s) 49, 122, 261, 287, 294
Mox20I TGGCCA 2 cut(s) 54, 152
Mph1103I ATGCAT 1 cut(s) 172
MscI TGGCCA 2 cut(s) 54, 152
MslI CAYNNNNRTG 1 cut(s) 62
Msp20I TGGCCA 2 cut(s) 54, 152
Mva1269I GAATGC 3 cut(s) 17, 170, 191
NdeII GATC 1 cut(s) 199
NlaIII CATG 5 cut(s) 53, 61, 109, 212, 264
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 256
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 2 cut(s) 212, 264
PceI AGGCCT 1 cut(s) 86
PciI ACATGT 2 cut(s) 208, 260
PctI GAATGC 3 cut(s) 17, 170, 191
PscI ACATGT 2 cut(s) 208, 260
PspN4I GGNNCC 1 cut(s) 72
RsaI GTAC 2 cut(s) 139, 195
RsaNI GTAC 2 cut(s) 138, 194
RseI CAYNNNNRTG 1 cut(s) 62
Sau3AI GATC 1 cut(s) 199
SetI ASST 4 cut(s) 31, 95, 225, 305
SfaNI GCATC 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 2 cut(s) 40, 298
SmoI CTYRAG 2 cut(s) 40, 298
Sse9I AATT 1 cut(s) 123
SseBI AGGCCT 1 cut(s) 86
SsiI CCGC 1 cut(s) 135
SspMI CTAG 1 cut(s) 131
StuI AGGCCT 1 cut(s) 86
TaaI ACNGT 2 cut(s) 148, 193
TasI AATT 1 cut(s) 123
TatI WGTACW 1 cut(s) 193
TscAI CASTG 2 cut(s) 153, 196
TseFI GTSAC 1 cut(s) 256
Tsp45I GTSAC 1 cut(s) 256
TspRI CASTG 2 cut(s) 153, 196
XceI RCATGY 2 cut(s) 212, 264
XspI CTAG 1 cut(s) 131
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.