RLG00000014021

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0019436
Physical Location & Seq
Reverse (-)
45847820 .. 45850074
2255 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014021

Sequence Viewer

Length: 1179 bp
ATGATTGGCCTTGCACTCCTTCAAGGCCAGAACTCAGAACCTTCGTCCCTGACTCTTCAACTTGATCGATCACAGCTCCAAAGCTTTTCGTCTTGGTTAAAGATATCAAAAATGGACAGAAGGAAATCCACACCTGATGTTTCGCCGGAATCTGCGGGTGATTCCCAGAGTGCAAACTTGCAATCCTTCATCCCCAATAACCATACCCAATCGGTCTTCACAGCGCATACTACCAGCACAGACCAACACATACTCGTCAGGCTTGGCTCTTTTTTTGATTCGCTGCTCGCTGGTAAGGCAATTATAGACACAGTATGCTCGGAATATGTGCAACTATCGAGTATGAAGAATGACAAACTCAAACATGTTTTCCTCAAGGATTATAATGAATATAGCTCCAATATGCTGAGTTTTTATTCTGAATTGGATGCGTTCATAAAATTAGCTCGTGAAAAGCATTCATTTATTGAATATACGGTTAACCGTTGTAGAAATACGGAATCTGATATGGGATTGGAGGACTTGATGGGGATTTCAGAAACATTGAAGCGTATCCAGGCTTCTTTTGATGAGACTTGTAACAGACTAGTGCTTGAAAAGGTTCAGCCTTTGTTGGATCAACAGGACAGATGGTTTAGTACTGTTAGTCTCGAAATAGAGAAACTTGGTCAGAAAGAAGCTTCTACCCGTTGTTTTAACCAGGCTAACGAAATACTATCAATGGTGATTTGTTCTGGTAAGGTCATTTCCAAGATCTTTACAGCATATGGAAGCTCTAGAGTTATCGATTCATTTTTTGCTGTTATCAAAGAAGAAGCGGCTTCTCTATGTCATTTGGTTTCCAAAGTTTTTAAAGCATGTGCAGGGGTTAAACTTCAAGATAAGGAGATTTATAACTCATACATCCAAAAAGAAACGATGGCTGGTGTTATGGAATTGACCGAATTTAAGCTTTTCATTGGTAACATTGGGAATGCGATTGGTTCAGTTGTTGTGAATGATGAGTATGCATCTCAAGGTTATGTCGATTTCGCGATGACAAATTTGAAAAGTAAGCTGAACAATTATTTGGAGACAGTTAAGGAATTCGAGGTCAAAATAGCTGCATGCCGGGACAAAACTATGAAAGCAAGGGAAGACATTAAGACCTCTAGTTTGAAGTATAAAATCGATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

393

Amino Acids

44.13

Weight (kDa)

6.15

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019436)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0267181
rosa_laevigata RLG00000014021
rosa_multiflora Rmu_ssc0000388.1_g000046
rosa_samantha Rh6AG152200 Rh6BG152400 Rh6CG148100 Rh6DG136600
rosa_wichuraiana Rw6G013130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 384, 894
AccII CGCG 1 cut(s) 1034
AciI CCGC 2 cut(s) 155, 818
AclWI GGATC 1 cut(s) 624
AcsI RAATTY 3 cut(s) 944, 1042, 1085
AfaI GTAC 1 cut(s) 640
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 8 cut(s) 23, 59, 470, 547, 596, 878, 1048, 1159
AhlI ACTAGT 1 cut(s) 586
AjnI CCWGG 2 cut(s) 555, 699
AjuI GAANNNNNNNTTGG 2 cut(s) 1052, 1084
AluBI AGCT 9 cut(s) 76, 84, 396, 446, 680, 774, 952, 1057, 1103
AluI AGCT 9 cut(s) 76, 84, 396, 446, 680, 774, 952, 1057, 1103
Alw26I GTCTC 3 cut(s) 566, 653, 1067
AlwI GGATC 1 cut(s) 624
AoxI GGCC 2 cut(s) 7, 25
ApeKI GCWGC 2 cut(s) 283, 1103
ApoI RAATTY 3 cut(s) 944, 1042, 1085
ArsI GACNNNNNNTTYG 2 cut(s) 1138, 1170
Asp700I GAANNNNTTC 1 cut(s) 600
AspLEI GCGC 1 cut(s) 226
AsuC2I CCSGG 1 cut(s) 1112
AsuHPI GGTGA 2 cut(s) 170, 736
BarI GAAGNNNNNNTAC 2 cut(s) 544, 576
BauI CACGAG 1 cut(s) 447
BbsI GAAGAC 2 cut(s) 208, 1143
BbvI GCAGC 2 cut(s) 270, 1090
BccI CCATC 3 cut(s) 520, 624, 913
BciT130I CCWGG 2 cut(s) 557, 701
BciVI GTATCC 1 cut(s) 563
BcnI CCSGG 1 cut(s) 1112
BcoDI GTCTC 3 cut(s) 566, 653, 1067
BcuI ACTAGT 1 cut(s) 586
BfaI CTAG 3 cut(s) 587, 777, 1152
BfuI GTATCC 1 cut(s) 563
BglII AGATCT 1 cut(s) 753
BisI GCNGC 3 cut(s) 284, 819, 1104
BlsI GCNGC 3 cut(s) 285, 820, 1105
BmcAI AGTACT 1 cut(s) 640
Bme1390I CCNGG 3 cut(s) 557, 701, 1112
BmrFI CCNGG 3 cut(s) 557, 701, 1112
BmsI GCATC 2 cut(s) 418, 1019
BpiI GAAGAC 2 cut(s) 208, 1143
BpuEI CTTGAG 2 cut(s) 359, 999
BpuMI CCSGG 1 cut(s) 1112
Bsa29I ATCGAT 3 cut(s) 67, 786, 1170
BseBI CCWGG 2 cut(s) 557, 701
BseCI ATCGAT 3 cut(s) 67, 786, 1170
BseGI GGATG 3 cut(s) 189, 433, 903
BseMII CTCAG 2 cut(s) 48, 398
BseXI GCAGC 2 cut(s) 270, 1090
BsgI GTGCAG 1 cut(s) 882
Bsh1236I CGCG 1 cut(s) 1034
BshFI GGCC 2 cut(s) 9, 27
BshVI ATCGAT 3 cut(s) 67, 786, 1170
BsiSI CCGG 2 cut(s) 146, 1111
BslFI GGGAC 2 cut(s) 31, 1127
BsmAI GTCTC 3 cut(s) 566, 653, 1067
BsmFI GGGAC 2 cut(s) 31, 1127
BsmI GAATGC 2 cut(s) 457, 979
BsnI GGCC 2 cut(s) 9, 27
Bsp143I GATC 4 cut(s) 64, 68, 616, 753
Bsp68I TCGCGA 1 cut(s) 1034
BspACI CCGC 2 cut(s) 155, 818
BspANI GGCC 2 cut(s) 9, 27
BspCNI CTCAG 2 cut(s) 47, 399
BspDI ATCGAT 3 cut(s) 67, 786, 1170
BspFNI CGCG 1 cut(s) 1034
BspPI GGATC 1 cut(s) 624
BssMI GATC 4 cut(s) 64, 68, 616, 753
BssSI CACGAG 1 cut(s) 447
Bst2BI CACGAG 1 cut(s) 447
Bst2UI CCWGG 2 cut(s) 557, 701
Bst4CI ACNGT 5 cut(s) 313, 478, 485, 643, 1078
Bst6I CTCTTC 1 cut(s) 60
BstC8I GCNNGC 2 cut(s) 288, 1108
BstDEI CTNAG 2 cut(s) 34, 407
BstF5I GGATG 3 cut(s) 189, 433, 903
BstFNI CGCG 1 cut(s) 1034
BstHHI GCGC 1 cut(s) 226
BstKTI GATC 4 cut(s) 67, 71, 619, 756
BstMAI GTCTC 3 cut(s) 566, 653, 1067
BstMBI GATC 4 cut(s) 64, 68, 616, 753
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstNI CCWGG 2 cut(s) 557, 701
BstNSI RCATGY 3 cut(s) 368, 861, 1110
BstSCI CCNGG 3 cut(s) 555, 699, 1110
BstUI CGCG 1 cut(s) 1034
BstV1I GCAGC 2 cut(s) 270, 1090
BstV2I GAAGAC 2 cut(s) 208, 1143
BstX2I RGATCY 1 cut(s) 753
BstYI RGATCY 1 cut(s) 753
Bsu15I ATCGAT 3 cut(s) 67, 786, 1170
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 2 cut(s) 9, 27
BsuTUI ATCGAT 3 cut(s) 67, 786, 1170
BtgZI GCGATG 1 cut(s) 1049
BtsCI GGATG 3 cut(s) 189, 433, 903
BtuMI TCGCGA 1 cut(s) 1034
Cac8I GCNNGC 2 cut(s) 288, 1108
CfoI GCGC 1 cut(s) 226
ClaI ATCGAT 3 cut(s) 67, 786, 1170
Csp6I GTAC 1 cut(s) 639
CviAII CATG 3 cut(s) 365, 858, 1107
CviQI GTAC 1 cut(s) 639
DdeI CTNAG 2 cut(s) 34, 407
DpnI GATC 4 cut(s) 66, 70, 618, 755
DpnII GATC 4 cut(s) 64, 68, 616, 753
DraI TTTAAA 1 cut(s) 853
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Eco32I GATATC 1 cut(s) 105
EcoRI GAATTC 1 cut(s) 1085
EcoRII CCWGG 2 cut(s) 555, 699
EcoRV GATATC 1 cut(s) 105
EcoT22I ATGCAT 1 cut(s) 1012
FaeI CATG 3 cut(s) 368, 861, 1110
FaqI GGGAC 2 cut(s) 31, 1127
FatI CATG 3 cut(s) 364, 857, 1106
FauI CCCGC 1 cut(s) 148
FauNDI CATATG 1 cut(s) 766
Fnu4HI GCNGC 3 cut(s) 284, 819, 1104
FokI GGATG 3 cut(s) 176, 440, 890
Fsp4HI GCNGC 3 cut(s) 284, 819, 1104
FspBI CTAG 3 cut(s) 587, 777, 1152
GlaI GCGC 1 cut(s) 225
GluI GCNGC 3 cut(s) 284, 819, 1104
HaeIII GGCC 2 cut(s) 9, 27
HapII CCGG 2 cut(s) 146, 1111
HhaI GCGC 1 cut(s) 226
Hin1II CATG 3 cut(s) 368, 861, 1110
Hin6I GCGC 1 cut(s) 224
HinP1I GCGC 1 cut(s) 224
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HindIII AAGCTT 3 cut(s) 82, 678, 950
HinfI GANTC 6 cut(s) 52, 149, 161, 278, 500, 788
HpaI GTTAAC 1 cut(s) 481
HpaII CCGG 2 cut(s) 146, 1111
HphI GGTGA 2 cut(s) 170, 736
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 6 cut(s) 37, 322, 421, 505, 538, 672
Hpy188III TCNNGA 5 cut(s) 449, 650, 777, 878, 1033
Hpy8I GTNNAC 1 cut(s) 481
HpyAV CCTTC 4 cut(s) 29, 51, 114, 196
HpyCH4III ACNGT 5 cut(s) 313, 478, 485, 643, 1078
HpyCH4V TGCA 7 cut(s) 14, 173, 181, 331, 863, 1010, 1106
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 2 cut(s) 34, 407
Hsp92II CATG 3 cut(s) 368, 861, 1110
HspAI GCGC 1 cut(s) 224
KspAI GTTAAC 1 cut(s) 481
Kzo9I GATC 4 cut(s) 64, 68, 616, 753
LmnI GCTCC 2 cut(s) 81, 401
Lsp1109I GCAGC 2 cut(s) 270, 1090
LweI GCATC 2 cut(s) 418, 1019
MaeI CTAG 3 cut(s) 587, 777, 1152
MaeIII GTNAC 2 cut(s) 578, 962
MalI GATC 4 cut(s) 66, 70, 618, 755
MboI GATC 4 cut(s) 64, 68, 616, 753
MboII GAAGA 5 cut(s) 47, 208, 358, 824, 1148
MflI RGATCY 1 cut(s) 753
MluCI AATT 8 cut(s) 300, 422, 440, 935, 944, 1042, 1063, 1085
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 594
MnlI CCTC 4 cut(s) 383, 511, 1084, 1159
Mph1103I ATGCAT 1 cut(s) 1012
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 8 cut(s) 98, 480, 696, 852, 870, 948, 1080, 1143
MspI CCGG 2 cut(s) 146, 1111
MspR9I CCNGG 3 cut(s) 557, 701, 1112
Mva1269I GAATGC 2 cut(s) 457, 979
MvaI CCWGG 2 cut(s) 557, 701
MvnI CGCG 1 cut(s) 1034
MwoI GCNNNNNNNGC 1 cut(s) 296
NciI CCSGG 1 cut(s) 1112
NdeI CATATG 1 cut(s) 766
NdeII GATC 4 cut(s) 64, 68, 616, 753
NlaIII CATG 3 cut(s) 368, 861, 1110
NruI TCGCGA 1 cut(s) 1034
NsiI ATGCAT 1 cut(s) 1012
NspI RCATGY 3 cut(s) 368, 861, 1110
PaeI GCATGC 1 cut(s) 1110
PciI ACATGT 1 cut(s) 364
PctI GAATGC 2 cut(s) 457, 979
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 5 cut(s) 149, 161, 278, 500, 788
PkrI GCNGC 3 cut(s) 285, 820, 1105
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
PscI ACATGT 1 cut(s) 364
PsiI TTATAA 2 cut(s) 384, 894
Psp6I CCWGG 2 cut(s) 555, 699
PspGI CCWGG 2 cut(s) 555, 699
PsuI RGATCY 1 cut(s) 753
RruI TCGCGA 1 cut(s) 1034
RsaI GTAC 1 cut(s) 640
RsaNI GTAC 1 cut(s) 639
SaqAI TTAA 8 cut(s) 98, 480, 696, 852, 870, 948, 1080, 1143
SatI GCNGC 3 cut(s) 284, 819, 1104
Sau3AI GATC 4 cut(s) 64, 68, 616, 753
ScaI AGTACT 1 cut(s) 640
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 3 cut(s) 557, 701, 1112
SfaNI GCATC 2 cut(s) 418, 1019
SmlI CTYRAG 2 cut(s) 374, 1014
SmoI CTYRAG 2 cut(s) 374, 1014
SpeI ACTAGT 1 cut(s) 586
SphI GCATGC 1 cut(s) 1110
Sse9I AATT 8 cut(s) 300, 422, 440, 935, 944, 1042, 1063, 1085
SsiI CCGC 2 cut(s) 155, 818
SspMI CTAG 3 cut(s) 587, 777, 1152
StyD4I CCNGG 3 cut(s) 555, 699, 1110
TaaI ACNGT 5 cut(s) 313, 478, 485, 643, 1078
TaqI TCGA 7 cut(s) 67, 338, 651, 786, 1026, 1089, 1170
TaqII GACCGA 2 cut(s) 202, 956
TasI AATT 8 cut(s) 300, 422, 440, 935, 944, 1042, 1063, 1085
TatI WGTACW 1 cut(s) 638
TauI GCSGC 1 cut(s) 821
TfiI GAWTC 5 cut(s) 149, 161, 278, 500, 788
Tru1I TTAA 8 cut(s) 98, 480, 696, 852, 870, 948, 1080, 1143
Tru9I TTAA 8 cut(s) 98, 480, 696, 852, 870, 948, 1080, 1143
TseI GCWGC 2 cut(s) 283, 1103
TspDTI ATGAA 8 cut(s) 178, 359, 402, 424, 450, 780, 946, 1139
TspGWI ACGGA 1 cut(s) 512
XapI RAATTY 3 cut(s) 944, 1042, 1085
XbaI TCTAGA 1 cut(s) 776
XceI RCATGY 3 cut(s) 368, 861, 1110
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 3 cut(s) 587, 777, 1152
ZrmI AGTACT 1 cut(s) 640
Zsp2I ATGCAT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.