Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0014179

Physical Location & Seq

Genomic Coordinates Reverse (-)

8536256 .. 8538607

Gene Structure 2352 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016645

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1566 bp

ATGATCAAACGAGAATCCTGGAAGCTCCTAGGGATATCTGCCAATATTTTCATAAATGGGGCCCAAATGTCACCCGTGGCAGTAGAAGATGGAGCCTGCACCAATATTTGTCAAGGTTTTTTGAGAGGCGTTCATGGTGGCTTTGCATGCAACTGGGGAACGCGGGCGACACACCCATTGCCACCTGATATAGTTGTGAGGCTTTTGAAGGACAATGGGTTCACCAAGGTGAAGCTATTTGAAGCTGATCCTGGGGCACTTGAAGCTCTAGGAGATTCCGGTATCCAGGTTATGCTGGGAATACCCAATGAGTTCTTGGCACCACTTGCAAGTACTGTTACGATCGCTGAAACGTGGGTGGCTCAGAATGTATCAAAATACATGTCCAAATACGGGGTCGATATAAGGTATGTGGCTGTAGGAAATGAACCCTTCCTCAAAAGCTACAAAGACGCATTCCTCAACACCACATTTCCAGCACTCCAAAACATTCAAGCAGCCCTCATAAAAGCCGGGCTAGGGAGACAAGTGAAGGTTACTGTTCCACTAAACGCCGACGTCTACCAGACCGACAGCGGTCTTCCCTCCGGAGGCAACTTCAGGTCCGACATCCACAGCCTCATACTCTCCATGATGAAGTTCCTCAGTGACAATGGTGGTGCTCTCACCATCAACATCTACCCCTTCCTTAGTCTCCAAGCTGACCCTGATTTCCCCAAAGAATATGCCTTCTTCGACAAAGGTGCAAAACCTGTTGTGGATGGTTCTATTTCCTACACCAATGTTCTTGACGCCAATTTTGACACCTGCATTGCTGCTCTGGAAAAGAATGGCTTCTCCTCTTTGCCTGTTATTGTAGGAGAGATCGGGTGGCCGACTGATGGTGACCCAAACGCTAACATAGAAAATGCAAAAAGATTCAATCAAGGGCTTGTGCGTCGTATACTTGCAGGGCAAGGCAGCCCAAAAAGGTCAACCCCGCCTGATATATACATATTCGGACTCATAGATGAAGATGCCAAGAGCGTCCAACCTGGGAATTTTGAGCGGCATTGGGGCGTCTTCAATTATGATGGATCACTCAAATACTCATTGGATATAGGTAAAGGAAAAGCTTTGGTTCCGGCGAAAGGAGTGCAATACTTGGCAAGACGATGGTGCATAATGTCACCTTCAGCGAGCACTTCCGACCCCAATTTGGTCCAGAGCATCGACTATGCTTGCACTTATGCCGATTGCACTAGTCTTGGATATGGCTCTTCTTGTGGAATGCTTGACCCAAGGAGTAATGCTTCTTATGCTTTCAATATGTACTACCAAACCATGGACCAACGCAAAGATGCATGCAATTTCTCGAGTCTGTCGATCACCACAAGTGTTGATCCATCTCCACCGTCGAATGGCCAGAACAGGACGTGTCAGTTTAAGATCATGATCGATCTGTCGAAGCACCAGAAAGCTAAACGGCCTCCTTCTTCAGTGGCTGCAGGACTAAATGAGCATAGCTCCCAAAAATTTGTGATGGCTTTACTTGTCCTTGTATTAGTGTCCACATTCATCATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001871 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0016787 GO:0016798 GO:0030054 GO:0030246 GO:0030247 GO:0030312 GO:0031224 GO:0031225 GO:0031226 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_17 X8

Protein Analysis

522

Amino Acids

56.55

Weight (kDa)

7.49

Isoelectric Point (pI)

44.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_17	PF00332	49 - 366	7.2e-61	Glycosyl hydrolases family 17
X8	PF07983	386 - 453	5.5e-15	X8 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014179)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20870
fragaria_vesca	FvH4_1g09660
malus_domestica	MD02G1105500.v1.1 MD15G1225400.v1.1
prunus_persica	Prupe.7G189600_v2.0.a1
pyrus_communis	pycom02g08350 pycom15g20020
rosa_chinensis	RchiOBHm_Chr2g0096501
rosa_laevigata	RLG00000016645
rosa_multiflora	Rmu_sc0004096.1_g000011
rosa_roxburghii	Rroxscaffold_2G00145590
rosa_rugosa	Rorug02G0058200.1
rosa_samantha	Rh2AG105200 Rh2BG107300 Rh2CG109100 Rh2DG108300
rosa_wichuraiana	Rw2G008130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	815
AatII	GACGTC	1 cut(s)	561
Acc36I	ACCTGC	1 cut(s)	815
AccB1I	GGYRCC	1 cut(s)	319
AccB7I	CCANNNNNTGG	1 cut(s)	1324
AccBSI	CCGCTC	1 cut(s)	1048
AccI	GTMKAC	2 cut(s)	561, 943
AccII	CGCG	1 cut(s)	163
AccIII	TCCGGA	1 cut(s)	587
AciI	CCGC	4 cut(s)	163, 576, 980, 1048
AclWI	GGATC	3 cut(s)	242, 1084, 1376
AcoI	YGGCCR	2 cut(s)	872, 1402
AcsI	RAATTY	2 cut(s)	1039, 1514
AcuI	CTGAAG	3 cut(s)	583, 1158, 1461
AcyI	GRCGYC	3 cut(s)	558, 792, 1059
AfaI	GTAC	2 cut(s)	334, 1313
AfiI	CCNNNNNNNGG	8 cut(s)	393, 519, 590, 881, 1130, 1198, 1324, 1400
AflIII	ACRYGT	2 cut(s)	381, 1415
AgsI	TTSAA	7 cut(s)	208, 242, 263, 494, 922, 1066, 1306
AhlI	ACTAGT	1 cut(s)	1241
AjiI	CACGTC	1 cut(s)	1416
AjnI	CCWGG	4 cut(s)	17, 250, 285, 1033
AleI	CACNNNNGTG	1 cut(s)	227
AloI	GAACNNNNNNTCC	2 cut(s)	203, 235
AluBI	AGCT	9 cut(s)	25, 235, 245, 266, 444, 701, 1115, 1460, 1506
AluI	AGCT	9 cut(s)	25, 235, 245, 266, 444, 701, 1115, 1460, 1506
Alw21I	GWGCWC	2 cut(s)	664, 1184
Alw26I	GTCTC	2 cut(s)	517, 698
AlwI	GGATC	3 cut(s)	242, 1084, 1376
AlwNI	CAGNNNCTG	1 cut(s)	1484
Ama87I	CYCGRG	1 cut(s)	1354
Aor13HI	TCCGGA	1 cut(s)	587
AoxI	GGCC	4 cut(s)	60, 872, 1402, 1466
ApaI	GGGCCC	1 cut(s)	64
ApeKI	GCWGC	4 cut(s)	497, 815, 960, 1484
ApoI	RAATTY	2 cut(s)	1039, 1514
ArsI	GACNNNNNNTTYG	6 cut(s)	368, 381, 400, 413, 782, 814
Asp700I	GAANNNNTTC	1 cut(s)	833
AspA2I	CCTAGG	1 cut(s)	28
AspS9I	GGNCC	5 cut(s)	60, 61, 603, 1201, 1327
AsuC2I	CCSGG	1 cut(s)	514
AsuHPI	GGTGA	7 cut(s)	63, 214, 241, 658, 896, 1161, 1360
AvaI	CYCGRG	1 cut(s)	1354
AvaII	GGWCC	3 cut(s)	603, 1201, 1327
AvrII	CCTAGG	1 cut(s)	28
BaeGI	GKGCMC	2 cut(s)	64, 259
BalI	TGGCCA	1 cut(s)	1404
BanI	GGYRCC	1 cut(s)	319
BanII	GRGCYC	1 cut(s)	64
BbsI	GAAGAC	2 cut(s)	572, 1054
Bbv12I	GWGCWC	2 cut(s)	664, 1184
BbvI	GCAGC	4 cut(s)	509, 802, 972, 1471
BccI	CCATC	8 cut(s)	83, 677, 755, 875, 1067, 1149, 1393, 1516
BceAI	ACGGC	1 cut(s)	1481
BcgI	CGANNNNNNTGC	4 cut(s)	725, 759, 1117, 1151
BciT130I	CCWGG	4 cut(s)	19, 252, 287, 1035
BciVI	GTATCC	1 cut(s)	293
BclI	TGATCA	1 cut(s)	3
BcnI	CCSGG	1 cut(s)	514
BcoDI	GTCTC	2 cut(s)	517, 698
BcuI	ACTAGT	1 cut(s)	1241
BfaI	CTAG	4 cut(s)	29, 269, 518, 1242
BfmI	CTRYAG	2 cut(s)	417, 1485
BfuAI	ACCTGC	1 cut(s)	815
BfuI	GTATCC	1 cut(s)	293
BisI	GCNGC	5 cut(s)	498, 816, 961, 1049, 1485
BlnI	CCTAGG	1 cut(s)	28
BlsI	GCNGC	5 cut(s)	499, 817, 962, 1050, 1486
BmcAI	AGTACT	1 cut(s)	334
Bme1390I	CCNGG	5 cut(s)	19, 252, 287, 514, 1035
Bme18I	GGWCC	3 cut(s)	603, 1201, 1327
BmeT110I	CYCGRG	1 cut(s)	1354
BmgBI	CACGTC	1 cut(s)	1416
BmgT120I	GGNCC	5 cut(s)	60, 61, 603, 1201, 1327
BmiI	GGNNCC	5 cut(s)	61, 62, 94, 321, 1122
BmrFI	CCNGG	5 cut(s)	19, 252, 287, 514, 1035
BmrI	ACTGGG	1 cut(s)	163
BmsI	GCATC	3 cut(s)	1006, 1218, 1330
BmuI	ACTGGG	1 cut(s)	163
BoxI	GACNNNNGTC	1 cut(s)	576
BpiI	GAAGAC	2 cut(s)	572, 1054
BplI	GAGNNNNNCTC	2 cut(s)	1490, 1522
BpuMI	CCSGG	1 cut(s)	514
Bsa29I	ATCGAT	1 cut(s)	1437
BsaBI	GATNNNNATC	1 cut(s)	1433
BsaHI	GRCGYC	3 cut(s)	558, 792, 1059
BsaJI	CCNNGG	7 cut(s)	28, 75, 225, 251, 1034, 1280, 1323
BsaWI	WCCGGW	2 cut(s)	278, 587
Bsc4I	CCNNNNNNNGG	8 cut(s)	393, 519, 590, 881, 1130, 1198, 1324, 1400
Bse1I	ACTGG	1 cut(s)	158
Bse3DI	GCAATG	2 cut(s)	176, 810
Bse8I	GATNNNNATC	1 cut(s)	1433
BseAI	TCCGGA	1 cut(s)	587
BseBI	CCWGG	4 cut(s)	19, 252, 287, 1035
BseCI	ATCGAT	1 cut(s)	1437
BseDI	CCNNGG	7 cut(s)	28, 75, 225, 251, 1034, 1280, 1323
BseGI	GGATG	2 cut(s)	609, 766
BseJI	GATNNNNATC	1 cut(s)	1433
BseLI	CCNNNNNNNGG	8 cut(s)	393, 519, 590, 881, 1130, 1198, 1324, 1400
BseMI	GCAATG	2 cut(s)	176, 810
BseMII	CTCAG	2 cut(s)	377, 658
BseNI	ACTGG	1 cut(s)	158
BseRI	GAGGAG	1 cut(s)	829
BseSI	GKGCMC	2 cut(s)	64, 259
BseXI	GCAGC	4 cut(s)	509, 802, 972, 1471
BseYI	CCCAGC	1 cut(s)	295
BsgI	GTGCAG	1 cut(s)	82
Bsh1236I	CGCG	1 cut(s)	163
Bsh1285I	CGRYCG	1 cut(s)	345
BshFI	GGCC	4 cut(s)	62, 874, 1404, 1468
BshNI	GGYRCC	1 cut(s)	319
BshVI	ATCGAT	1 cut(s)	1437
BsiEI	CGRYCG	1 cut(s)	345
BsiHKAI	GWGCWC	2 cut(s)	664, 1184
BsiHKCI	CYCGRG	1 cut(s)	1354
BsiSI	CCGG	4 cut(s)	279, 513, 588, 1124
BslI	CCNNNNNNNGG	8 cut(s)	393, 519, 590, 881, 1130, 1198, 1324, 1400
BsmAI	GTCTC	2 cut(s)	517, 698
BsmI	GAATGC	2 cut(s)	455, 1275
BsnI	GGCC	4 cut(s)	62, 874, 1404, 1468
BsoBI	CYCGRG	1 cut(s)	1354
Bsp120I	GGGCCC	1 cut(s)	60
Bsp1286I	GDGCHC	4 cut(s)	64, 259, 664, 1184
Bsp13I	TCCGGA	1 cut(s)	587
Bsp19I	CCATGG	1 cut(s)	1323
BspACI	CCGC	4 cut(s)	163, 576, 980, 1048
BspANI	GGCC	4 cut(s)	62, 874, 1404, 1468
BspCNI	CTCAG	2 cut(s)	376, 657
BspDI	ATCGAT	1 cut(s)	1437
BspEI	TCCGGA	1 cut(s)	587
BspFNI	CGCG	1 cut(s)	163
BspHI	TCATGA	1 cut(s)	1431
BspLI	GGNNCC	5 cut(s)	61, 62, 94, 321, 1122
BspMAI	CTGCAG	1 cut(s)	1489
BspMI	ACCTGC	1 cut(s)	815
BspPI	GGATC	3 cut(s)	242, 1084, 1376
BspQI	GCTCTTC	1 cut(s)	1264
BspT107I	GGYRCC	1 cut(s)	319
BsrBI	CCGCTC	1 cut(s)	1048
BsrDI	GCAATG	2 cut(s)	176, 810
BsrI	ACTGG	1 cut(s)	158
BssECI	CCNNGG	7 cut(s)	28, 75, 225, 251, 1034, 1280, 1323
BssNAI	GTATAC	1 cut(s)	944
BssNI	GRCGYC	3 cut(s)	558, 792, 1059
BssT1I	CCWWGG	4 cut(s)	28, 225, 1280, 1323
Bst1107I	GTATAC	1 cut(s)	944
Bst2UI	CCWGG	4 cut(s)	19, 252, 287, 1035
Bst4CI	ACNGT	3 cut(s)	337, 541, 1395
Bst6I	CTCTTC	1 cut(s)	1264
BstACI	GRCGYC	3 cut(s)	558, 792, 1059
BstAPI	GCANNNNNTGC	1 cut(s)	326
BstC8I	GCNNGC	6 cut(s)	97, 148, 165, 1180, 1222, 1345
BstDEI	CTNAG	3 cut(s)	363, 644, 689
BstDSI	CCRYGG	2 cut(s)	75, 1323
BstEII	GGTNACC	1 cut(s)	884
BstF5I	GGATG	2 cut(s)	609, 766
BstFNI	CGCG	1 cut(s)	163
BstMAI	GTCTC	2 cut(s)	517, 698
BstMCI	CGRYCG	1 cut(s)	345
BstMWI	GCNNNNNNNGC	4 cut(s)	147, 263, 326, 1298
BstNI	CCWGG	4 cut(s)	19, 252, 287, 1035
BstNSI	RCATGY	3 cut(s)	150, 385, 1347
BstPAI	GACNNNNGTC	1 cut(s)	576
BstPI	GGTNACC	1 cut(s)	884
BstSCI	CCNGG	5 cut(s)	17, 250, 285, 512, 1033
BstSFI	CTRYAG	2 cut(s)	417, 1485
BstSLI	GKGCMC	2 cut(s)	64, 259
BstUI	CGCG	1 cut(s)	163
BstV1I	GCAGC	4 cut(s)	509, 802, 972, 1471
BstV2I	GAAGAC	2 cut(s)	572, 1054
BstZ17I	GTATAC	1 cut(s)	944
Bsu15I	ATCGAT	1 cut(s)	1437
BsuI	GTATCC	1 cut(s)	293
BsuRI	GGCC	4 cut(s)	62, 874, 1404, 1468
BsuTUI	ATCGAT	1 cut(s)	1437
BtgI	CCRYGG	2 cut(s)	75, 1323
BtrI	CACGTC	1 cut(s)	1416
BtsCI	GGATG	2 cut(s)	609, 766
BtsIMutI	CAGTG	2 cut(s)	652, 1485
BveI	ACCTGC	1 cut(s)	815
Cac8I	GCNNGC	6 cut(s)	97, 148, 165, 1180, 1222, 1345
CaiI	CAGNNNCTG	1 cut(s)	1484
CciI	TCATGA	1 cut(s)	1431
Cfr13I	GGNCC	5 cut(s)	60, 61, 603, 1201, 1327
ClaI	ATCGAT	1 cut(s)	1437
CseI	GACGC	5 cut(s)	461, 800, 926, 1015, 1048
Csp6I	GTAC	2 cut(s)	333, 1312
CspCI	CAANNNNNGTGG	2 cut(s)	1360, 1395
CviAII	CATG	7 cut(s)	134, 147, 382, 631, 1324, 1344, 1432
CviQI	GTAC	2 cut(s)	333, 1312
DdeI	CTNAG	3 cut(s)	363, 644, 689
EaeI	YGGCCR	2 cut(s)	872, 1402
Eam1104I	CTCTTC	1 cut(s)	1264
EarI	CTCTTC	1 cut(s)	1264
Eco130I	CCWWGG	4 cut(s)	28, 225, 1280, 1323
Eco24I	GRGCYC	1 cut(s)	64
Eco32I	GATATC	1 cut(s)	36
Eco47I	GGWCC	3 cut(s)	603, 1201, 1327
Eco57I	CTGAAG	3 cut(s)	583, 1158, 1461
Eco88I	CYCGRG	1 cut(s)	1354
Eco91I	GGTNACC	1 cut(s)	884
EcoO109I	RGGNCCY	1 cut(s)	60
EcoO65I	GGTNACC	1 cut(s)	884
EcoRII	CCWGG	4 cut(s)	17, 250, 285, 1033
EcoRV	GATATC	1 cut(s)	36
EcoT14I	CCWWGG	4 cut(s)	28, 225, 1280, 1323
EcoT22I	ATGCAT	1 cut(s)	1345
EcoT38I	GRGCYC	1 cut(s)	64
ErhI	CCWWGG	4 cut(s)	28, 225, 1280, 1323
FaeI	CATG	7 cut(s)	137, 150, 385, 634, 1327, 1347, 1435
FalI	AAGNNNNNCTT	2 cut(s)	818, 850
FatI	CATG	7 cut(s)	133, 146, 381, 630, 1323, 1343, 1431
FauI	CCCGC	2 cut(s)	156, 987
FbaI	TGATCA	1 cut(s)	3
FblI	GTMKAC	2 cut(s)	561, 943
Fnu4HI	GCNGC	5 cut(s)	498, 816, 961, 1049, 1485
FokI	GGATG	2 cut(s)	596, 773
FriOI	GRGCYC	1 cut(s)	64
Fsp4HI	GCNGC	5 cut(s)	498, 816, 961, 1049, 1485
FspBI	CTAG	4 cut(s)	29, 269, 518, 1242
GluI	GCNGC	5 cut(s)	498, 816, 961, 1049, 1485
GsaI	CCCAGC	1 cut(s)	299
HaeIII	GGCC	4 cut(s)	62, 874, 1404, 1468
HapII	CCGG	4 cut(s)	279, 513, 588, 1124
HgaI	GACGC	5 cut(s)	461, 800, 926, 1015, 1048
Hin1I	GRCGYC	3 cut(s)	558, 792, 1059
Hin1II	CATG	7 cut(s)	137, 150, 385, 634, 1327, 1347, 1435
HincII	GTYRAC	1 cut(s)	975
HindII	GTYRAC	1 cut(s)	975
HindIII	AAGCTT	1 cut(s)	1113
HinfI	GANTC	5 cut(s)	14, 275, 918, 1002, 1357
HpaII	CCGG	4 cut(s)	279, 513, 588, 1124
HphI	GGTGA	7 cut(s)	63, 214, 241, 658, 896, 1161, 1360
Hpy166II	GTNNAC	5 cut(s)	222, 562, 944, 975, 1551
Hpy188I	TCNGA	4 cut(s)	366, 607, 1001, 1189
Hpy188III	TCNNGA	6 cut(s)	588, 788, 821, 1204, 1354, 1432
Hpy8I	GTNNAC	5 cut(s)	222, 562, 944, 975, 1551
Hpy99I	CGWCG	3 cut(s)	560, 942, 1399
HpyAV	CCTTC	7 cut(s)	202, 442, 526, 694, 739, 1182, 1482
HpyCH4III	ACNGT	3 cut(s)	337, 541, 1395
HpyCH4IV	ACGT	3 cut(s)	353, 558, 1415
HpyF10VI	GCNNNNNNNGC	4 cut(s)	147, 263, 326, 1298
HpyF3I	CTNAG	3 cut(s)	363, 644, 689
HpySE526I	ACGT	3 cut(s)	353, 558, 1415
Hsp92I	GRCGYC	3 cut(s)	558, 792, 1059
Hsp92II	CATG	7 cut(s)	137, 150, 385, 634, 1327, 1347, 1435
Kpn2I	TCCGGA	1 cut(s)	587
Ksp22I	TGATCA	1 cut(s)	3
LguI	GCTCTTC	1 cut(s)	1264
LmnI	GCTCC	3 cut(s)	30, 92, 1511
Lsp1109I	GCAGC	4 cut(s)	509, 802, 972, 1471
LweI	GCATC	3 cut(s)	1006, 1218, 1330
MaeI	CTAG	4 cut(s)	29, 269, 518, 1242
MaeII	ACGT	3 cut(s)	353, 558, 1415
MaeIII	GTNAC	6 cut(s)	69, 337, 535, 647, 884, 1167
MbiI	CCGCTC	1 cut(s)	1048
MboII	GAAGA	7 cut(s)	98, 572, 724, 1025, 1054, 1251, 1467
MhlI	GDGCHC	4 cut(s)	64, 259, 664, 1184
MlsI	TGGCCA	1 cut(s)	1404
MluCI	AATT	6 cut(s)	796, 1039, 1066, 1195, 1348, 1514
MluNI	TGGCCA	1 cut(s)	1404
MlyI	GAGTC	2 cut(s)	996, 1366
MmeI	TCCRAC	3 cut(s)	630, 1054, 1212
Mox20I	TGGCCA	1 cut(s)	1404
Mph1103I	ATGCAT	1 cut(s)	1345
MroI	TCCGGA	1 cut(s)	587
MroXI	GAANNNNTTC	1 cut(s)	833
MscI	TGGCCA	1 cut(s)	1404
MseI	TTAA	1 cut(s)	1425
MslI	CAYNNNNRTG	1 cut(s)	227
Msp20I	TGGCCA	1 cut(s)	1404
MspA1I	CMGCKG	1 cut(s)	576
MspI	CCGG	4 cut(s)	279, 513, 588, 1124
MspR9I	CCNGG	5 cut(s)	19, 252, 287, 514, 1035
Mva1269I	GAATGC	2 cut(s)	455, 1275
MvaI	CCWGG	4 cut(s)	19, 252, 287, 1035
MvnI	CGCG	1 cut(s)	163
MwoI	GCNNNNNNNGC	4 cut(s)	147, 263, 326, 1298
NciI	CCSGG	1 cut(s)	514
NcoI	CCATGG	1 cut(s)	1323
NlaIII	CATG	7 cut(s)	137, 150, 385, 634, 1327, 1347, 1435
NlaIV	GGNNCC	5 cut(s)	61, 62, 94, 321, 1122
NmuCI	GTSAC	4 cut(s)	69, 647, 884, 1167
NsiI	ATGCAT	1 cut(s)	1345
NspI	RCATGY	3 cut(s)	150, 385, 1347
OliI	CACNNNNGTG	1 cut(s)	227
PaeI	GCATGC	2 cut(s)	150, 1347
PaeR7I	CTCGAG	1 cut(s)	1354
PagI	TCATGA	1 cut(s)	1431
PaqCI	CACCTGC	1 cut(s)	815
PciI	ACATGT	1 cut(s)	381
PciSI	GCTCTTC	1 cut(s)	1264
PcsI	WCGNNNNNNNCGW	1 cut(s)	1361
PctI	GAATGC	2 cut(s)	455, 1275
PdmI	GAANNNNTTC	1 cut(s)	833
PfeI	GAWTC	3 cut(s)	14, 275, 918
PflMI	CCANNNNNTGG	1 cut(s)	1324
PfoI	TCCNGGA	1 cut(s)	17
PkrI	GCNGC	5 cut(s)	499, 817, 962, 1050, 1486
Ple19I	CGATCG	1 cut(s)	345
PleI	GAGTC	2 cut(s)	996, 1365
PpsI	GAGTC	2 cut(s)	996, 1365
PscI	ACATGT	1 cut(s)	381
PshAI	GACNNNNGTC	1 cut(s)	576
Psp6I	CCWGG	4 cut(s)	17, 250, 285, 1033
PspEI	GGTNACC	1 cut(s)	884
PspFI	CCCAGC	1 cut(s)	295
PspGI	CCWGG	4 cut(s)	17, 250, 285, 1033
PspN4I	GGNNCC	5 cut(s)	61, 62, 94, 321, 1122
PspOMI	GGGCCC	1 cut(s)	60
PspPI	GGNCC	5 cut(s)	60, 61, 603, 1201, 1327
PstI	CTGCAG	1 cut(s)	1489
PstNI	CAGNNNCTG	1 cut(s)	1484
PvuI	CGATCG	1 cut(s)	345
RsaI	GTAC	2 cut(s)	334, 1313
RsaNI	GTAC	2 cut(s)	333, 1312
RseI	CAYNNNNRTG	1 cut(s)	227
SapI	GCTCTTC	1 cut(s)	1264
SaqAI	TTAA	1 cut(s)	1425
SatI	GCNGC	5 cut(s)	498, 816, 961, 1049, 1485
Sau96I	GGNCC	5 cut(s)	60, 61, 603, 1201, 1327
ScaI	AGTACT	1 cut(s)	334
SchI	GAGTC	2 cut(s)	996, 1366
ScrFI	CCNGG	5 cut(s)	19, 252, 287, 514, 1035
SduI	GDGCHC	4 cut(s)	64, 259, 664, 1184
SfaNI	GCATC	3 cut(s)	1006, 1218, 1330
SfcI	CTRYAG	2 cut(s)	417, 1485
Sfr274I	CTCGAG	1 cut(s)	1354
SinI	GGWCC	3 cut(s)	603, 1201, 1327
SlaI	CTCGAG	1 cut(s)	1354
SmiMI	CAYNNNNRTG	1 cut(s)	227
SmlI	CTYRAG	1 cut(s)	1354
SmoI	CTYRAG	1 cut(s)	1354
SpeI	ACTAGT	1 cut(s)	1241
SphI	GCATGC	2 cut(s)	150, 1347
Sse9I	AATT	6 cut(s)	796, 1039, 1066, 1195, 1348, 1514
SsiI	CCGC	4 cut(s)	163, 576, 980, 1048
SspI	AATATT	2 cut(s)	46, 106
SspMI	CTAG	4 cut(s)	29, 269, 518, 1242
StyD4I	CCNGG	5 cut(s)	17, 250, 285, 512, 1033
StyI	CCWWGG	4 cut(s)	28, 225, 1280, 1323
TaaI	ACNGT	3 cut(s)	337, 541, 1395
TaiI	ACGT	3 cut(s)	356, 561, 1418
TaqI	TCGA	8 cut(s)	399, 735, 1212, 1355, 1364, 1397, 1437, 1445
TaqII	GACCGA	1 cut(s)	584
TasI	AATT	6 cut(s)	796, 1039, 1066, 1195, 1348, 1514
TatI	WGTACW	2 cut(s)	332, 1311
TauI	GCSGC	1 cut(s)	1051
TfiI	GAWTC	3 cut(s)	14, 275, 918
Tru1I	TTAA	1 cut(s)	1425
Tru9I	TTAA	1 cut(s)	1425
TscAI	CASTG	2 cut(s)	652, 1485
TseFI	GTSAC	4 cut(s)	69, 647, 884, 1167
TseI	GCWGC	4 cut(s)	497, 815, 960, 1484
Tsp45I	GTSAC	4 cut(s)	69, 647, 884, 1167
TspDTI	ATGAA	6 cut(s)	40, 122, 441, 650, 1026, 1546
TspRI	CASTG	2 cut(s)	652, 1485
Van91I	CCANNNNNTGG	1 cut(s)	1324
VpaK11BI	GGWCC	3 cut(s)	603, 1201, 1327
XapI	RAATTY	2 cut(s)	1039, 1514
XceI	RCATGY	3 cut(s)	150, 385, 1347
XcmI	CCANNNNNNNNNTGG	1 cut(s)	313
XhoI	CTCGAG	1 cut(s)	1354
XmaJI	CCTAGG	1 cut(s)	28
XmiI	GTMKAC	2 cut(s)	561, 943
XmnI	GAANNNNTTC	1 cut(s)	833
XspI	CTAG	4 cut(s)	29, 269, 518, 1242
ZraI	GACGTC	1 cut(s)	559
ZrmI	AGTACT	1 cut(s)	334
Zsp2I	ATGCAT	1 cut(s)	1345

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.