RLG00000016856

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0021561
Physical Location & Seq
Reverse (-)
10686980 .. 10688455
1476 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016856

Sequence Viewer

Length: 954 bp
ATGTGTGCTACAAAAAGCAACGCATGGTTGAAGGTGAAGGGGTGCGGGGTTCGCGCTCTCTACAAGCAAAGCTTTGAAGAACTCATACAACTCGACAGAAGAATGAACGAATACATAAGAATTTCCGAGCGGAATTTGGTTAGTATGATGCAGACACCCCTTTCTTTTTCTGAGGTGGTCAGTTTCATAATTTCTGGAAATGATATTCCTGTGTGGTTCGAAACTAGTTCGAGGGCAGACTTAACGATGGGGTGGCAACCTGACAATGATAGTCCGACGTGGATGGGGTTTGCTTTATGCGTTCTCTTTGGAACTGAAGAAAAGTCTGGTGCCCTAGGTGAATACCATGATCTAGAACCTCATGTAGACAAAATTATATGTGGTTGGTGGCCCTGTGGCTTCCCTAATATGAACCTCCCCAGTTGCAGTGGTAATCAAGAACAAGTTGTTCAGTTATCAGATCACCTTTGTGTATTCTTCGTTCCTAGGGATAAATTGATGGGTTCATGGACATTTACCTTTGACTTCAAAACCAAGTGTTCTAACAGCAACGCATGCTTGAAGGTGAAAAGGTGCGGGGTCCTTAGTTTGTACGAGGAAGACTACTTGGAAGAGACCAACATACGGAGTCTATTCAGTGCACATCCATGTGTGCATGGAATCCGTTTCCACAGAAGAGAGAACCATTACCAATCCATCATGTTTAAGAGGAATCGGTTCGCAGATTGCTTGGAACGAACTTATGTCGACAACGAAATGATGCCGTGTAGTTGGGTCATGAAGAAGTTGGCATCAGTGCTAGTAGGTCAAGAATTGAGAGAGGAGTGCCGCGAGCTCAAAAATTCTCCTTCGGCAGCGGAAGAGGTTCTCGATGACGTGCAATTTGATGTTCATTGTGTTGCTGGGACTATAGTATTTGGGCGTTCTATTGTCGGGACTCGGAATTGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

318

Amino Acids

36.37

Weight (kDa)

5.88

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 65 - 201 5.6e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021561)

Species Orthologous Gene IDs
rosa_laevigata RLG00000016856
rosa_rugosa Rorug02G0077800
rosa_samantha Rh2AG126600 Rh2CG130800 Rh2DG131200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 329
AccBSI CCGCTC 1 cut(s) 130
AccI GTMKAC 2 cut(s) 366, 747
AccII CGCG 2 cut(s) 54, 831
AciI CCGC 5 cut(s) 45, 130, 576, 829, 857
AcsI RAATTY 3 cut(s) 120, 133, 841
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 1 cut(s) 624
AgsI TTSAA 4 cut(s) 31, 77, 529, 562
AhlI ACTAGT 1 cut(s) 224
AjiI CACGTC 2 cut(s) 279, 877
AleI CACNNNNGTG 1 cut(s) 468
AluBI AGCT 2 cut(s) 72, 835
AluI AGCT 2 cut(s) 72, 835
Alw21I GWGCWC 2 cut(s) 643, 837
Alw26I GTCTC 1 cut(s) 608
Alw44I GTGCAC 1 cut(s) 639
AoxI GGCC 1 cut(s) 389
ApaLI GTGCAC 1 cut(s) 639
ApeKI GCWGC 1 cut(s) 854
ApoI RAATTY 3 cut(s) 120, 133, 841
ArsI GACNNNNNNTTYG 2 cut(s) 866, 898
Asp700I GAANNNNTTC 2 cut(s) 716, 864
AspA2I CCTAGG 2 cut(s) 334, 485
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 2 cut(s) 390, 580
AsuHPI GGTGA 4 cut(s) 46, 350, 455, 577
AsuII TTCGAA 1 cut(s) 219
AvaII GGWCC 1 cut(s) 580
AvrII CCTAGG 2 cut(s) 334, 485
BaeGI GKGCMC 2 cut(s) 334, 643
BanI GGYRCC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 837
BarI GAAGNNNNNNTAC 2 cut(s) 69, 101
BbsI GAAGAC 1 cut(s) 606
Bbv12I GWGCWC 2 cut(s) 643, 837
BbvI GCAGC 1 cut(s) 866
BccI CCATC 4 cut(s) 241, 277, 493, 704
BceAI ACGGC 1 cut(s) 748
BcoDI GTCTC 1 cut(s) 608
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 6 cut(s) 225, 335, 353, 486, 800, 952
BfmI CTRYAG 1 cut(s) 909
BisI GCNGC 2 cut(s) 829, 855
BlnI CCTAGG 2 cut(s) 334, 485
BlsI GCNGC 2 cut(s) 830, 856
Bme18I GGWCC 1 cut(s) 580
BmgBI CACGTC 2 cut(s) 279, 877
BmgT120I GGNCC 2 cut(s) 390, 580
BmiI GGNNCC 2 cut(s) 331, 581
BmrI ACTGGG 1 cut(s) 414
BmsI GCATC 3 cut(s) 138, 750, 800
BmuI ACTGGG 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 606
Bpu14I TTCGAA 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 608
BsaJI CCNNGG 2 cut(s) 334, 485
Bsc4I CCNNNNNNNGG 1 cut(s) 624
Bse1I ACTGG 1 cut(s) 420
BseDI CCNNGG 2 cut(s) 334, 485
BseGI GGATG 2 cut(s) 288, 643
BseLI CCNNNNNNNGG 1 cut(s) 624
BseMII CTCAG 1 cut(s) 162
BseNI ACTGG 1 cut(s) 420
BseRI GAGGAG 1 cut(s) 836
BseSI GKGCMC 2 cut(s) 334, 643
BseXI GCAGC 1 cut(s) 866
BseYI CCCAGC 1 cut(s) 902
Bsh1236I CGCG 2 cut(s) 54, 831
BshFI GGCC 1 cut(s) 391
BshNI GGYRCC 1 cut(s) 329
BsiHKAI GWGCWC 2 cut(s) 643, 837
BslFI GGGAC 2 cut(s) 919, 949
BslI CCNNNNNNNGG 1 cut(s) 624
BsmAI GTCTC 1 cut(s) 608
BsmFI GGGAC 2 cut(s) 919, 949
BsnI GGCC 1 cut(s) 391
Bso31I GGTCTC 1 cut(s) 608
Bsp119I TTCGAA 1 cut(s) 219
Bsp1286I GDGCHC 3 cut(s) 334, 643, 837
Bsp143I GATC 2 cut(s) 349, 460
BspACI CCGC 5 cut(s) 45, 130, 576, 829, 857
BspANI GGCC 1 cut(s) 391
BspCNI CTCAG 1 cut(s) 163
BspFNI CGCG 2 cut(s) 54, 831
BspHI TCATGA 1 cut(s) 777
BspLI GGNNCC 2 cut(s) 331, 581
BspT104I TTCGAA 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 329
BspTNI GGTCTC 1 cut(s) 608
BsrBI CCGCTC 1 cut(s) 130
BsrI ACTGG 1 cut(s) 420
BssECI CCNNGG 2 cut(s) 334, 485
BssMI GATC 2 cut(s) 349, 460
BssT1I CCWWGG 2 cut(s) 334, 485
Bst6I CTCTTC 3 cut(s) 606, 670, 855
BstAPI GCANNNNNTGC 1 cut(s) 555
BstBI TTCGAA 1 cut(s) 219
BstC8I GCNNGC 2 cut(s) 556, 833
BstDEI CTNAG 2 cut(s) 171, 584
BstF5I GGATG 2 cut(s) 288, 643
BstFNI CGCG 2 cut(s) 54, 831
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 2 cut(s) 352, 463
BstMAI GTCTC 1 cut(s) 608
BstMBI GATC 2 cut(s) 349, 460
BstMWI GCNNNNNNNGC 2 cut(s) 51, 555
BstNSI RCATGY 1 cut(s) 558
BstSFI CTRYAG 1 cut(s) 909
BstSLI GKGCMC 2 cut(s) 334, 643
BstUI CGCG 2 cut(s) 54, 831
BstV1I GCAGC 1 cut(s) 866
BstV2I GAAGAC 1 cut(s) 606
BsuRI GGCC 1 cut(s) 391
BtrI CACGTC 2 cut(s) 279, 877
BtsCI GGATG 2 cut(s) 288, 643
BtsI GCAGTG 1 cut(s) 433
BtsIMutI CAGTG 3 cut(s) 433, 643, 801
Cac8I GCNNGC 2 cut(s) 556, 833
CciI TCATGA 1 cut(s) 777
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 2 cut(s) 390, 580
Csp6I GTAC 1 cut(s) 592
CviAII CATG 9 cut(s) 24, 347, 362, 507, 555, 648, 656, 700, 778
CviJI RGCY 4 cut(s) 72, 391, 399, 835
CviKI_1 RGCY 4 cut(s) 72, 391, 399, 835
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 2 cut(s) 171, 584
DpnI GATC 2 cut(s) 351, 462
DpnII GATC 2 cut(s) 349, 460
Eam1104I CTCTTC 3 cut(s) 606, 670, 855
EarI CTCTTC 3 cut(s) 606, 670, 855
Ecl136II GAGCTC 1 cut(s) 835
Eco130I CCWWGG 2 cut(s) 334, 485
Eco24I GRGCYC 1 cut(s) 837
Eco31I GGTCTC 1 cut(s) 608
Eco47I GGWCC 1 cut(s) 580
Eco53kI GAGCTC 1 cut(s) 835
Eco57I CTGAAG 1 cut(s) 336
EcoICRI GAGCTC 1 cut(s) 835
EcoO109I RGGNCCY 1 cut(s) 580
EcoT14I CCWWGG 2 cut(s) 334, 485
EcoT38I GRGCYC 1 cut(s) 837
ErhI CCWWGG 2 cut(s) 334, 485
FaeI CATG 9 cut(s) 27, 350, 365, 510, 558, 651, 659, 703, 781
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FaqI GGGAC 2 cut(s) 919, 949
FatI CATG 9 cut(s) 23, 346, 361, 506, 554, 647, 655, 699, 777
FauI CCCGC 2 cut(s) 38, 569
FblI GTMKAC 2 cut(s) 366, 747
Fnu4HI GCNGC 2 cut(s) 829, 855
FokI GGATG 2 cut(s) 295, 630
FriOI GRGCYC 1 cut(s) 837
Fsp4HI GCNGC 2 cut(s) 829, 855
FspBI CTAG 6 cut(s) 225, 335, 353, 486, 800, 952
GlaI GCGC 1 cut(s) 55
GluI GCNGC 2 cut(s) 829, 855
GsaI CCCAGC 1 cut(s) 906
HaeIII GGCC 1 cut(s) 391
HhaI GCGC 1 cut(s) 56
Hin1II CATG 9 cut(s) 27, 350, 365, 510, 558, 651, 659, 703, 781
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HindIII AAGCTT 1 cut(s) 70
HinfI GANTC 4 cut(s) 628, 660, 712, 937
HphI GGTGA 4 cut(s) 46, 350, 455, 577
Hpy166II GTNNAC 3 cut(s) 367, 641, 748
Hpy188I TCNGA 5 cut(s) 127, 172, 276, 460, 942
Hpy188III TCNNGA 7 cut(s) 195, 353, 437, 778, 809, 869, 934
Hpy8I GTNNAC 3 cut(s) 367, 641, 748
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 4 cut(s) 25, 31, 556, 858
HpyCH4IV ACGT 2 cut(s) 278, 876
HpyCH4V TGCA 5 cut(s) 151, 426, 641, 655, 880
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 555
HpyF3I CTNAG 2 cut(s) 171, 584
HpySE526I ACGT 2 cut(s) 278, 876
Hsp92II CATG 9 cut(s) 27, 350, 365, 510, 558, 651, 659, 703, 781
HspAI GCGC 1 cut(s) 54
Kzo9I GATC 2 cut(s) 349, 460
LpnPI CCDG 7 cut(s) 180, 222, 273, 312, 406, 433, 888
Lsp1109I GCAGC 1 cut(s) 866
LweI GCATC 3 cut(s) 138, 750, 800
MaeI CTAG 6 cut(s) 225, 335, 353, 486, 800, 952
MaeII ACGT 2 cut(s) 278, 876
MaeIII GTNAC 1 cut(s) 947
MalI GATC 2 cut(s) 351, 462
MbiI CCGCTC 1 cut(s) 130
MboI GATC 2 cut(s) 349, 460
MboII GAAGA 9 cut(s) 89, 111, 329, 469, 611, 623, 687, 793, 872
MhlI GDGCHC 3 cut(s) 334, 643, 837
MluCI AATT 9 cut(s) 120, 133, 189, 372, 494, 812, 841, 881, 943
MlyI GAGTC 2 cut(s) 637, 931
MmeI TCCRAC 1 cut(s) 299
MnlI CCTC 8 cut(s) 166, 225, 369, 425, 589, 702, 814, 856
MroXI GAANNNNTTC 2 cut(s) 716, 864
MseI TTAA 2 cut(s) 242, 705
MslI CAYNNNNRTG 3 cut(s) 468, 646, 648
MspA1I CMGCKG 1 cut(s) 857
MvnI CGCG 2 cut(s) 54, 831
MwoI GCNNNNNNNGC 2 cut(s) 51, 555
NdeII GATC 2 cut(s) 349, 460
NlaIII CATG 9 cut(s) 27, 350, 365, 510, 558, 651, 659, 703, 781
NlaIV GGNNCC 2 cut(s) 331, 581
NmuCI GTSAC 1 cut(s) 947
NspI RCATGY 1 cut(s) 558
NspV TTCGAA 1 cut(s) 219
OliI CACNNNNGTG 1 cut(s) 468
PaeI GCATGC 1 cut(s) 558
PagI TCATGA 1 cut(s) 777
PdmI GAANNNNTTC 2 cut(s) 716, 864
PfeI GAWTC 2 cut(s) 660, 712
PkrI GCNGC 2 cut(s) 830, 856
PleI GAGTC 2 cut(s) 636, 931
PpsI GAGTC 2 cut(s) 636, 931
PpuMI RGGWCCY 1 cut(s) 580
Psp124BI GAGCTC 1 cut(s) 837
Psp5II RGGWCCY 1 cut(s) 580
PspFI CCCAGC 1 cut(s) 902
PspN4I GGNNCC 2 cut(s) 331, 581
PspPI GGNCC 2 cut(s) 390, 580
PspPPI RGGWCCY 1 cut(s) 580
PsrI GAACNNNNNNTAC 2 cut(s) 465, 497
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
RseI CAYNNNNRTG 3 cut(s) 468, 646, 648
SacI GAGCTC 1 cut(s) 837
SalI GTCGAC 1 cut(s) 746
SaqAI TTAA 2 cut(s) 242, 705
SatI GCNGC 2 cut(s) 829, 855
Sau3AI GATC 2 cut(s) 349, 460
Sau96I GGNCC 2 cut(s) 390, 580
SchI GAGTC 2 cut(s) 637, 931
SduI GDGCHC 3 cut(s) 334, 643, 837
SfaNI GCATC 3 cut(s) 138, 750, 800
SfcI CTRYAG 1 cut(s) 909
SfuI TTCGAA 1 cut(s) 219
SinI GGWCC 1 cut(s) 580
SmiMI CAYNNNNRTG 3 cut(s) 468, 646, 648
SpeI ACTAGT 1 cut(s) 224
SphI GCATGC 1 cut(s) 558
Sse9I AATT 9 cut(s) 120, 133, 189, 372, 494, 812, 841, 881, 943
SsiI CCGC 5 cut(s) 45, 130, 576, 829, 857
SspMI CTAG 6 cut(s) 225, 335, 353, 486, 800, 952
SstI GAGCTC 1 cut(s) 837
StyI CCWWGG 2 cut(s) 334, 485
TaiI ACGT 2 cut(s) 281, 879
TaqI TCGA 5 cut(s) 93, 219, 230, 747, 870
TasI AATT 9 cut(s) 120, 133, 189, 372, 494, 812, 841, 881, 943
TauI GCSGC 1 cut(s) 831
TfiI GAWTC 2 cut(s) 660, 712
Tru1I TTAA 2 cut(s) 242, 705
Tru9I TTAA 2 cut(s) 242, 705
TscAI CASTG 3 cut(s) 433, 643, 801
TseFI GTSAC 1 cut(s) 947
TseI GCWGC 1 cut(s) 854
Tsp45I GTSAC 1 cut(s) 947
TspDTI ATGAA 6 cut(s) 119, 175, 425, 495, 794, 881
TspGWI ACGGA 2 cut(s) 640, 653
TspRI CASTG 3 cut(s) 433, 643, 801
VneI GTGCAC 1 cut(s) 639
VpaK11BI GGWCC 1 cut(s) 580
XapI RAATTY 3 cut(s) 120, 133, 841
XbaI TCTAGA 1 cut(s) 352
XceI RCATGY 1 cut(s) 558
XmaJI CCTAGG 2 cut(s) 334, 485
XmiI GTMKAC 2 cut(s) 366, 747
XmnI GAANNNNTTC 2 cut(s) 716, 864
XspI CTAG 6 cut(s) 225, 335, 353, 486, 800, 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.