Rh2CG130800

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0021561
Physical Location & Seq
Reverse (-)
11651616 .. 11652119
504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG130800.1

Sequence Viewer

Length: 504 bp
ATGGTGCAGGAACCCCCTCCTTTTTATGAGATTTTCAGTACTGTAATTCCTGGAAATGACATTCCTGTGTGGTTCGAAACTAGAAGGAAGCGAGCAGACTCAGTGAGCCAATTAGAGCGACCTTGTATCTCATGTTGTAATACGTGGATGGGGTTTGCTTTATGTGTTCTTTTTGGAGCTGAAGAAATCCCAAGTGAATTCCCACATACACATCGAGCAAACAGTACCATATGGTGTAGTTGGAAGGCCAATCATTTCTCTCTCGCCAGATACAGTTGTGCTGTTAATTCATCAGATCACCTTTGTGTATTCTTTATTCCTGCGGATAAATTTCTGCATTTACCAAAACGGGTCCACTTTGAGTTCAAAAGCAGCGATGCATACTTGAAGGTGAAAGGGTACGGGGTCCGTTCTTTGTACGAGCATGACTTGCCCAGGAATTTGGCCAACGCGAGTTTGAAAGGACAACTGCTCCGGTCAATAAACAATTGCAGATCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

167

Amino Acids

19.15

Weight (kDa)

8.65

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 16 - 144 3.3e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021561)

Species Orthologous Gene IDs
rosa_laevigata RLG00000016856
rosa_rugosa Rorug02G0077800
rosa_samantha Rh2AG126600 Rh2CG130800 Rh2DG131200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 452
AciI CCGC 1 cut(s) 323
AcoI YGGCCR 1 cut(s) 444
AcsI RAATTY 3 cut(s) 197, 329, 439
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 4 cut(s) 40, 226, 401, 419
AgsI TTSAA 3 cut(s) 367, 388, 460
AjnI CCWGG 2 cut(s) 49, 434
AleI CACNNNNGTG 1 cut(s) 303
AluBI AGCT 1 cut(s) 179
AluI AGCT 1 cut(s) 179
AoxI GGCC 2 cut(s) 246, 444
ApeKI GCWGC 1 cut(s) 372
ApoI RAATTY 3 cut(s) 197, 329, 439
AspS9I GGNCC 2 cut(s) 352, 406
AsuHPI GGTGA 2 cut(s) 290, 403
AsuII TTCGAA 1 cut(s) 75
AvaII GGWCC 2 cut(s) 352, 406
BaeI ACNNNNGTAYC 2 cut(s) 262, 295
BalI TGGCCA 1 cut(s) 446
BbvI GCAGC 1 cut(s) 384
BccI CCATC 1 cut(s) 142
BciT130I CCWGG 2 cut(s) 51, 436
BfaI CTAG 1 cut(s) 81
BglII AGATCT 1 cut(s) 494
BisI GCNGC 1 cut(s) 373
BlsI GCNGC 1 cut(s) 374
BmcAI AGTACT 1 cut(s) 40
Bme1390I CCNGG 2 cut(s) 51, 436
Bme18I GGWCC 2 cut(s) 352, 406
BmgT120I GGNCC 2 cut(s) 352, 406
BmiI GGNNCC 3 cut(s) 12, 353, 407
BmrFI CCNGG 2 cut(s) 51, 436
BmsI GCATC 1 cut(s) 367
Bpu14I TTCGAA 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 434
BsaWI WCCGGW 1 cut(s) 474
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
BseBI CCWGG 2 cut(s) 51, 436
BseDI CCNNGG 1 cut(s) 434
BseGI GGATG 1 cut(s) 153
BseMII CTCAG 1 cut(s) 114
BseXI GCAGC 1 cut(s) 384
BsgI GTGCAG 1 cut(s) 26
Bsh1236I CGCG 1 cut(s) 452
BshFI GGCC 2 cut(s) 248, 446
BsiSI CCGG 1 cut(s) 475
BsnI GGCC 2 cut(s) 248, 446
Bsp119I TTCGAA 1 cut(s) 75
Bsp143I GATC 2 cut(s) 295, 494
BspACI CCGC 1 cut(s) 323
BspANI GGCC 2 cut(s) 248, 446
BspCNI CTCAG 1 cut(s) 113
BspFNI CGCG 1 cut(s) 452
BspHI TCATGA 1 cut(s) 500
BspLI GGNNCC 3 cut(s) 12, 353, 407
BspT104I TTCGAA 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 434
BssMI GATC 2 cut(s) 295, 494
Bst2UI CCWGG 2 cut(s) 51, 436
Bst4CI ACNGT 3 cut(s) 43, 224, 275
BstAPI GCANNNNNTGC 1 cut(s) 430
BstBAI YACGTR 1 cut(s) 144
BstBI TTCGAA 1 cut(s) 75
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 1 cut(s) 100
BstF5I GGATG 1 cut(s) 153
BstFNI CGCG 1 cut(s) 452
BstKTI GATC 2 cut(s) 298, 497
BstMBI GATC 2 cut(s) 295, 494
BstMWI GCNNNNNNNGC 1 cut(s) 430
BstNI CCWGG 2 cut(s) 51, 436
BstSCI CCNGG 2 cut(s) 49, 434
BstUI CGCG 1 cut(s) 452
BstV1I GCAGC 1 cut(s) 384
BstX2I RGATCY 1 cut(s) 494
BstXI CCANNNNNNTGG 1 cut(s) 442
BstYI RGATCY 1 cut(s) 494
BsuRI GGCC 2 cut(s) 248, 446
BtgZI GCGATG 1 cut(s) 390
BtsCI GGATG 1 cut(s) 153
BtsIMutI CAGTG 1 cut(s) 108
Cac8I GCNNGC 1 cut(s) 93
CciI TCATGA 1 cut(s) 500
Cfr13I GGNCC 2 cut(s) 352, 406
Csp6I GTAC 4 cut(s) 39, 225, 400, 418
CviAII CATG 3 cut(s) 132, 425, 501
CviJI RGCY 4 cut(s) 108, 179, 248, 446
CviKI_1 RGCY 4 cut(s) 108, 179, 248, 446
CviQI GTAC 4 cut(s) 39, 225, 400, 418
DdeI CTNAG 1 cut(s) 100
DpnI GATC 2 cut(s) 297, 496
DpnII GATC 2 cut(s) 295, 494
EaeI YGGCCR 1 cut(s) 444
Eco47I GGWCC 2 cut(s) 352, 406
Eco57I CTGAAG 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 2 cut(s) 49, 434
EcoT22I ATGCAT 1 cut(s) 382
FaeI CATG 3 cut(s) 135, 428, 504
FaiI YATR 9 cut(s) 27, 133, 163, 207, 230, 232, 382, 426, 502
FatI CATG 3 cut(s) 131, 424, 500
FauNDI CATATG 1 cut(s) 230
Fnu4HI GCNGC 1 cut(s) 373
FokI GGATG 1 cut(s) 160
Fsp4HI GCNGC 1 cut(s) 373
FspBI CTAG 1 cut(s) 81
GluI GCNGC 1 cut(s) 373
HaeIII GGCC 2 cut(s) 248, 446
HapII CCGG 1 cut(s) 475
Hin1II CATG 3 cut(s) 135, 428, 504
HinfI GANTC 1 cut(s) 98
HpaII CCGG 1 cut(s) 475
HphI GGTGA 2 cut(s) 290, 403
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 1 cut(s) 295
Hpy188III TCNNGA 1 cut(s) 501
Hpy8I GTNNAC 1 cut(s) 355
HpyAV CCTTC 3 cut(s) 78, 238, 382
HpyCH4III ACNGT 3 cut(s) 43, 224, 275
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 4 cut(s) 7, 337, 380, 492
HpyF10VI GCNNNNNNNGC 1 cut(s) 430
HpyF3I CTNAG 1 cut(s) 100
HpySE526I ACGT 1 cut(s) 143
Hsp92II CATG 3 cut(s) 135, 428, 504
Kzo9I GATC 2 cut(s) 295, 494
LmnI GCTCC 2 cut(s) 176, 477
LpnPI CCDG 8 cut(s) 36, 63, 78, 280, 333, 421, 448, 488
Lsp1109I GCAGC 1 cut(s) 384
LweI GCATC 1 cut(s) 367
MaeI CTAG 1 cut(s) 81
MaeII ACGT 1 cut(s) 143
MalI GATC 2 cut(s) 297, 496
MboI GATC 2 cut(s) 295, 494
MboII GAAGA 2 cut(s) 194, 489
MfeI CAATTG 1 cut(s) 487
MflI RGATCY 1 cut(s) 494
MlsI TGGCCA 1 cut(s) 446
MluCI AATT 7 cut(s) 45, 110, 197, 286, 329, 439, 487
MluNI TGGCCA 1 cut(s) 446
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 221
MnlI CCTC 1 cut(s) 27
Mox20I TGGCCA 1 cut(s) 446
Mph1103I ATGCAT 1 cut(s) 382
MscI TGGCCA 1 cut(s) 446
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 2 cut(s) 65, 303
Msp20I TGGCCA 1 cut(s) 446
MspI CCGG 1 cut(s) 475
MspR9I CCNGG 2 cut(s) 51, 436
MunI CAATTG 1 cut(s) 487
MvaI CCWGG 2 cut(s) 51, 436
MvnI CGCG 1 cut(s) 452
MwoI GCNNNNNNNGC 1 cut(s) 430
NdeI CATATG 1 cut(s) 230
NdeII GATC 2 cut(s) 295, 494
NlaIII CATG 3 cut(s) 135, 428, 504
NlaIV GGNNCC 3 cut(s) 12, 353, 407
NsiI ATGCAT 1 cut(s) 382
NspV TTCGAA 1 cut(s) 75
OliI CACNNNNGTG 1 cut(s) 303
PagI TCATGA 1 cut(s) 500
PfoI TCCNGGA 1 cut(s) 49
PkrI GCNGC 1 cut(s) 374
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Ppu21I YACGTR 1 cut(s) 144
Psp6I CCWGG 2 cut(s) 49, 434
PspGI CCWGG 2 cut(s) 49, 434
PspN4I GGNNCC 3 cut(s) 12, 353, 407
PspPI GGNCC 2 cut(s) 352, 406
PsuI RGATCY 1 cut(s) 494
RsaI GTAC 4 cut(s) 40, 226, 401, 419
RsaNI GTAC 4 cut(s) 39, 225, 400, 418
RseI CAYNNNNRTG 2 cut(s) 65, 303
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 1 cut(s) 373
Sau3AI GATC 2 cut(s) 295, 494
Sau96I GGNCC 2 cut(s) 352, 406
ScaI AGTACT 1 cut(s) 40
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 2 cut(s) 51, 436
SetI ASST 5 cut(s) 124, 146, 181, 303, 393
SfaNI GCATC 1 cut(s) 367
SfuI TTCGAA 1 cut(s) 75
SinI GGWCC 2 cut(s) 352, 406
SmiMI CAYNNNNRTG 2 cut(s) 65, 303
Sse9I AATT 7 cut(s) 45, 110, 197, 286, 329, 439, 487
SsiI CCGC 1 cut(s) 323
SspMI CTAG 1 cut(s) 81
StyD4I CCNGG 2 cut(s) 49, 434
TaaI ACNGT 3 cut(s) 43, 224, 275
TaiI ACGT 1 cut(s) 146
TaqI TCGA 2 cut(s) 75, 214
TasI AATT 7 cut(s) 45, 110, 197, 286, 329, 439, 487
TatI WGTACW 1 cut(s) 38
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 1 cut(s) 108
TseI GCWGC 1 cut(s) 372
TspDTI ATGAA 2 cut(s) 279, 489
TspGWI ACGGA 1 cut(s) 398
TspRI CASTG 1 cut(s) 108
VpaK11BI GGWCC 2 cut(s) 352, 406
XapI RAATTY 3 cut(s) 197, 329, 439
XspI CTAG 1 cut(s) 81
ZrmI AGTACT 1 cut(s) 40
Zsp2I ATGCAT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.