RLG00000024628

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0030843
Physical Location & Seq
Reverse (-)
37591484 .. 37594372
2889 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024628

Sequence Viewer

Length: 843 bp
ATGGTGTTGCCGACGAGGGCTGCGTCTCTTCTCTCTCCGATTCTTCTCTCAACCGCTGGCTGCGTCTTCTCCAATTCTTTGGTCTCAACCTCGCCGGAGATCGGATTCTCAACCACGGCGAAGAAGAAATACAAGGAGTTGCAGAGACCGAGAGTGGAACAAGAAGAACACAAAGAAGAGGATCAAAACGACGTCGCTCTGGATCGCCTTTTGCTCGTCACTTTGGCCATCAAATTTGATGGAATAAGCTTTATGAACTTGAATTTAATGGGAGGGATTGCTTCTGCGCTTCATTATCTACACGAAGGGTTTGAGCAGGTTGTGGTTCACAGAGATGTCAAGGCTAGCAATGTGTTGCTCGATAGTGAAATGAATGGGAGGCTTGGGAATTTTGGGATGGCGAGGTTGCATGAACTAGGGTCGAAATCGAATACGACTTGGGTCGTGGGTACGCTTGGGTATTTGGCTCCGGAGTTGCCCAGAACAGGGAAGGTGAGTATGGGTTCGGATGTGTATGCATTTGGCACGCTTTTGCTGGAGGTGGTTTGCGGGTTGAGCCCAATGCAAAGCCCGAGGAGTTGGTTTTGGTGGATTGGAATTGACAGCCTTAGTTTAAATAGGTTTAAAGAGGGGAAGATACTTGAGGTGGTGGATCCCAAGCTTGATAGGGTTTATGATGAGAGGGAGGTGGTGATGGTAGTGAAGTTGGGGTTTATGTGCTCGGCCCATGGGCAGAGGGCCAGGCCCAATATGAGGCTAGTGGTGAGGTATTTGGACAGGGATCGAGGTGGAGGTTCTGGAGGAGCTGAGACCGCCGAGTGGGTCCCATGTGGCCTAGTTTGA

Protein Analysis

281

Amino Acids

30.94

Weight (kDa)

6.61

Isoelectric Point (pI)

26.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 87 - 194 2.1e-17 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 88 - 189 1.9e-21 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030843)

Species Orthologous Gene IDs
rosa_laevigata RLG00000024628
rosa_multiflora Rmu_sc0000395.1_g000019

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 195
Acc36I ACCTGC 1 cut(s) 307
AccIII TCCGGA 1 cut(s) 469
AciI CCGC 3 cut(s) 54, 549, 813
AclWI GGATC 5 cut(s) 189, 210, 647, 660, 789
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 3 cut(s) 233, 262, 388
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 1 cut(s) 451
AfiI CCNNNNNNNGG 5 cut(s) 101, 485, 486, 753, 819
AgsI TTSAA 1 cut(s) 262
AjnI CCWGG 1 cut(s) 740
AluBI AGCT 3 cut(s) 249, 661, 806
AluI AGCT 3 cut(s) 249, 661, 806
Alw21I GWGCWC 1 cut(s) 722
Alw26I GTCTC 4 cut(s) 30, 88, 139, 803
AlwI GGATC 5 cut(s) 189, 210, 647, 660, 789
Ama87I CYCGRG 1 cut(s) 571
Aor13HI TCCGGA 1 cut(s) 469
AoxI GGCC 5 cut(s) 225, 723, 738, 743, 832
ApeKI GCWGC 2 cut(s) 20, 60
ApoI RAATTY 3 cut(s) 233, 262, 388
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 4 cut(s) 724, 738, 744, 823
AsuHPI GGTGA 3 cut(s) 505, 703, 775
AsuNHI GCTAGC 1 cut(s) 344
AvaI CYCGRG 1 cut(s) 571
AvaII GGWCC 1 cut(s) 823
BalI TGGCCA 1 cut(s) 227
BamHI GGATCC 1 cut(s) 652
BanII GRGCYC 1 cut(s) 560
BarI GAAGNNNNNNTAC 2 cut(s) 113, 145
BbsI GAAGAC 1 cut(s) 58
Bbv12I GWGCWC 1 cut(s) 722
BbvI GCAGC 2 cut(s) 7, 47
BccI CCATC 4 cut(s) 233, 236, 391, 688
BceAI ACGGC 1 cut(s) 132
BciT130I CCWGG 1 cut(s) 742
BcoDI GTCTC 4 cut(s) 30, 88, 139, 803
BfaI CTAG 4 cut(s) 345, 416, 758, 836
BfuAI ACCTGC 1 cut(s) 307
BisI GCNGC 2 cut(s) 21, 61
BlsI GCNGC 2 cut(s) 22, 62
Bme1390I CCNGG 1 cut(s) 742
Bme18I GGWCC 1 cut(s) 823
BmeT110I CYCGRG 1 cut(s) 571
BmgT120I GGNCC 4 cut(s) 724, 738, 744, 823
BmiI GGNNCC 4 cut(s) 468, 654, 824, 825
BmrFI CCNGG 1 cut(s) 742
BmtI GCTAGC 1 cut(s) 348
BoxI GACNNNNGTC 1 cut(s) 440
BpiI GAAGAC 1 cut(s) 58
BpmI CTGGAG 2 cut(s) 557, 819
BpuEI CTTGAG 1 cut(s) 662
BsaHI GRCGYC 1 cut(s) 192
BsaI GGTCTC 3 cut(s) 88, 139, 803
BsaJI CCNNGG 3 cut(s) 114, 572, 727
BsaWI WCCGGW 1 cut(s) 469
Bsc4I CCNNNNNNNGG 5 cut(s) 101, 485, 486, 753, 819
Bse3DI GCAATG 1 cut(s) 355
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 1 cut(s) 742
BseDI CCNNGG 3 cut(s) 114, 572, 727
BseGI GGATG 2 cut(s) 402, 514
BseLI CCNNNNNNNGG 5 cut(s) 101, 485, 486, 753, 819
BseMI GCAATG 1 cut(s) 355
BseMII CTCAG 1 cut(s) 798
BseRI GAGGAG 2 cut(s) 589, 816
BseXI GCAGC 2 cut(s) 7, 47
BshFI GGCC 5 cut(s) 227, 725, 740, 745, 834
BsiHKAI GWGCWC 1 cut(s) 722
BsiHKCI CYCGRG 1 cut(s) 571
BsiSI CCGG 2 cut(s) 95, 470
BslFI GGGAC 1 cut(s) 809
BslI CCNNNNNNNGG 5 cut(s) 101, 485, 486, 753, 819
BsmAI GTCTC 4 cut(s) 30, 88, 139, 803
BsmBI CGTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 809
BsnI GGCC 5 cut(s) 227, 725, 740, 745, 834
Bso31I GGTCTC 3 cut(s) 88, 139, 803
BsoBI CYCGRG 1 cut(s) 571
Bsp1286I GDGCHC 2 cut(s) 560, 722
Bsp13I TCCGGA 1 cut(s) 469
Bsp143I GATC 5 cut(s) 99, 181, 202, 652, 781
Bsp19I CCATGG 1 cut(s) 727
BspACI CCGC 3 cut(s) 54, 549, 813
BspANI GGCC 5 cut(s) 227, 725, 740, 745, 834
BspCNI CTCAG 1 cut(s) 799
BspEI TCCGGA 1 cut(s) 469
BspLI GGNNCC 4 cut(s) 468, 654, 824, 825
BspMI ACCTGC 1 cut(s) 307
BspOI GCTAGC 1 cut(s) 348
BspPI GGATC 5 cut(s) 189, 210, 647, 660, 789
BspTNI GGTCTC 3 cut(s) 88, 139, 803
BsrDI GCAATG 1 cut(s) 355
BssECI CCNNGG 3 cut(s) 114, 572, 727
BssMI GATC 5 cut(s) 99, 181, 202, 652, 781
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 727
Bst2UI CCWGG 1 cut(s) 742
Bst6I CTCTTC 2 cut(s) 33, 171
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 3 cut(s) 58, 346, 527
BstDEI CTNAG 2 cut(s) 608, 807
BstDSI CCRYGG 2 cut(s) 114, 727
BstF5I GGATG 2 cut(s) 402, 514
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 5 cut(s) 102, 184, 205, 655, 784
BstMAI GTCTC 4 cut(s) 30, 88, 139, 803
BstMBI GATC 5 cut(s) 99, 181, 202, 652, 781
BstMWI GCNNNNNNNGC 2 cut(s) 555, 812
BstNI CCWGG 1 cut(s) 742
BstPAI GACNNNNGTC 1 cut(s) 440
BstSCI CCNGG 1 cut(s) 740
BstV1I GCAGC 2 cut(s) 7, 47
BstV2I GAAGAC 1 cut(s) 58
BstX2I RGATCY 1 cut(s) 652
BstXI CCANNNNNNTGG 1 cut(s) 79
BstYI RGATCY 1 cut(s) 652
BsuRI GGCC 5 cut(s) 227, 725, 740, 745, 834
BtgI CCRYGG 2 cut(s) 114, 727
BtsCI GGATG 2 cut(s) 402, 514
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 3 cut(s) 58, 346, 527
CfoI GCGC 1 cut(s) 289
Cfr13I GGNCC 4 cut(s) 724, 738, 744, 823
CseI GACGC 2 cut(s) 12, 52
Csp6I GTAC 1 cut(s) 450
CviAII CATG 3 cut(s) 410, 728, 828
CviQI GTAC 1 cut(s) 450
DdeI CTNAG 2 cut(s) 608, 807
DpnI GATC 5 cut(s) 101, 183, 204, 654, 783
DpnII GATC 5 cut(s) 99, 181, 202, 652, 781
DraI TTTAAA 2 cut(s) 615, 625
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 2 cut(s) 33, 171
EarI CTCTTC 2 cut(s) 33, 171
Eco130I CCWWGG 1 cut(s) 727
Eco24I GRGCYC 1 cut(s) 560
Eco31I GGTCTC 3 cut(s) 88, 139, 803
Eco47I GGWCC 1 cut(s) 823
Eco88I CYCGRG 1 cut(s) 571
EcoO109I RGGNCCY 1 cut(s) 823
EcoRII CCWGG 1 cut(s) 740
EcoT14I CCWWGG 1 cut(s) 727
EcoT22I ATGCAT 1 cut(s) 520
EcoT38I GRGCYC 1 cut(s) 560
ErhI CCWWGG 1 cut(s) 727
Esp3I CGTCTC 1 cut(s) 30
FaeI CATG 3 cut(s) 413, 731, 831
FaiI YATR 9 cut(s) 254, 411, 500, 516, 675, 716, 729, 752, 829
FaqI GGGAC 1 cut(s) 809
FatI CATG 3 cut(s) 409, 727, 827
FauI CCCGC 1 cut(s) 542
Fnu4HI GCNGC 2 cut(s) 21, 61
FokI GGATG 2 cut(s) 409, 521
FriOI GRGCYC 1 cut(s) 560
Fsp4HI GCNGC 2 cut(s) 21, 61
FspBI CTAG 4 cut(s) 345, 416, 758, 836
GlaI GCGC 1 cut(s) 288
GluI GCNGC 2 cut(s) 21, 61
GsuI CTGGAG 2 cut(s) 557, 819
HaeIII GGCC 5 cut(s) 227, 725, 740, 745, 834
HapII CCGG 2 cut(s) 95, 470
HgaI GACGC 2 cut(s) 12, 52
HhaI GCGC 1 cut(s) 289
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 3 cut(s) 413, 731, 831
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HindIII AAGCTT 2 cut(s) 247, 659
HinfI GANTC 2 cut(s) 40, 105
HpaII CCGG 2 cut(s) 95, 470
HphI GGTGA 3 cut(s) 505, 703, 775
Hpy166II GTNNAC 1 cut(s) 328
Hpy188I TCNGA 3 cut(s) 39, 104, 508
Hpy188III TCNNGA 3 cut(s) 200, 470, 798
Hpy8I GTNNAC 1 cut(s) 328
Hpy99I CGWCG 3 cut(s) 16, 194, 197
HpyAV CCTTC 2 cut(s) 299, 484
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 4 cut(s) 142, 409, 518, 565
HpyF10VI GCNNNNNNNGC 2 cut(s) 555, 812
HpyF3I CTNAG 2 cut(s) 608, 807
HpySE526I ACGT 1 cut(s) 192
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 3 cut(s) 413, 731, 831
HspAI GCGC 1 cut(s) 287
KflI GGGWCCC 1 cut(s) 823
Kpn2I TCCGGA 1 cut(s) 469
Kzo9I GATC 5 cut(s) 99, 181, 202, 652, 781
LmnI GCTCC 2 cut(s) 472, 803
Lsp1109I GCAGC 2 cut(s) 7, 47
MaeI CTAG 4 cut(s) 345, 416, 758, 836
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 217
MalI GATC 5 cut(s) 101, 183, 204, 654, 783
MboI GATC 5 cut(s) 99, 181, 202, 652, 781
MboII GAAGA 8 cut(s) 20, 35, 58, 133, 136, 176, 188, 646
MflI RGATCY 1 cut(s) 652
MhlI GDGCHC 2 cut(s) 560, 722
MlsI TGGCCA 1 cut(s) 227
MluCI AATT 5 cut(s) 73, 233, 262, 388, 597
MluNI TGGCCA 1 cut(s) 227
Mox20I TGGCCA 1 cut(s) 227
Mph1103I ATGCAT 1 cut(s) 520
MroI TCCGGA 1 cut(s) 469
MscI TGGCCA 1 cut(s) 227
MseI TTAA 3 cut(s) 266, 614, 624
MslI CAYNNNNRTG 1 cut(s) 333
Msp20I TGGCCA 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 2 cut(s) 95, 470
MspR9I CCNGG 1 cut(s) 742
MvaI CCWGG 1 cut(s) 742
MwoI GCNNNNNNNGC 2 cut(s) 555, 812
NcoI CCATGG 1 cut(s) 727
NdeII GATC 5 cut(s) 99, 181, 202, 652, 781
NheI GCTAGC 1 cut(s) 344
NlaIII CATG 3 cut(s) 413, 731, 831
NlaIV GGNNCC 4 cut(s) 468, 654, 824, 825
NmeAIII GCCGAG 2 cut(s) 701, 841
NmuCI GTSAC 1 cut(s) 217
NsiI ATGCAT 1 cut(s) 520
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PfeI GAWTC 2 cut(s) 40, 105
PkrI GCNGC 2 cut(s) 22, 62
PpuMI RGGWCCY 1 cut(s) 823
PshAI GACNNNNGTC 1 cut(s) 440
Psp5II RGGWCCY 1 cut(s) 823
Psp6I CCWGG 1 cut(s) 740
PspGI CCWGG 1 cut(s) 740
PspN4I GGNNCC 4 cut(s) 468, 654, 824, 825
PspPI GGNCC 4 cut(s) 724, 738, 744, 823
PspPPI RGGWCCY 1 cut(s) 823
PsuI RGATCY 1 cut(s) 652
RsaI GTAC 1 cut(s) 451
RsaNI GTAC 1 cut(s) 450
RseI CAYNNNNRTG 1 cut(s) 333
SaqAI TTAA 3 cut(s) 266, 614, 624
SatI GCNGC 2 cut(s) 21, 61
Sau3AI GATC 5 cut(s) 99, 181, 202, 652, 781
Sau96I GGNCC 4 cut(s) 724, 738, 744, 823
ScrFI CCNGG 1 cut(s) 742
SduI GDGCHC 2 cut(s) 560, 722
SinI GGWCC 1 cut(s) 823
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
Sse9I AATT 5 cut(s) 73, 233, 262, 388, 597
SsiI CCGC 3 cut(s) 54, 549, 813
SspMI CTAG 4 cut(s) 345, 416, 758, 836
StyD4I CCNGG 1 cut(s) 740
StyI CCWWGG 1 cut(s) 727
TaiI ACGT 1 cut(s) 195
TaqI TCGA 4 cut(s) 360, 422, 428, 784
TaqII GACCGA 1 cut(s) 163
TasI AATT 5 cut(s) 73, 233, 262, 388, 597
TfiI GAWTC 2 cut(s) 40, 105
Tru1I TTAA 3 cut(s) 266, 614, 624
Tru9I TTAA 3 cut(s) 266, 614, 624
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 2 cut(s) 20, 60
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 4 cut(s) 269, 281, 386, 426
VpaK11BI GGWCC 1 cut(s) 823
XapI RAATTY 3 cut(s) 233, 262, 388
XspI CTAG 4 cut(s) 345, 416, 758, 836
ZraI GACGTC 1 cut(s) 193
Zsp2I ATGCAT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.