RLG00000024644

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0025343
Physical Location & Seq
Reverse (-)
37804880 .. 37805527
648 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024644

Sequence Viewer

Length: 648 bp
ATGCCCTTGATCTTCTTCTTCTTTGTCTTCGTCATCCTCTTCGTCTTCTCCAATGTTCTCCACCCCATTCGTACTCTCCTTCGCCCTCCTCCTCTCTCCCCCCCCCCCCTCCTCTTCCTCCTCGCCCCTCATTTCCTCCCTCCCAAGAACCAAATCTCGATCCCTGCGGCTGATGAAATCGACGACCAAGCCCTCTTTGCCCGAGCCGTCCGATCCAAATCGGCATTCACCCGCCTCTCCCTCGATTCCAAAACCAAGCCCAAAATCGCCTTCCTCTTCCTCACCAACTCCGACCTCCACTTCGCCCCCCTCTGGCAACGCGTTCCTCTCCACTCCTTCCGCTATGTCTACCGCTCTCTCTTCGCCTCATCCACATTCGACAAGTCCCGGCCCGCCACCGAGTCTGACGTGGAGTTGACTCGGATGGGAGTCAAGGTCAAGTACAACAGTTTTATCGAAATCCTCTCCAAATTAACCAGTCTCTGGAAGCGGTACCCCGCCAGGGGAAGGTACGCCATGATGTCGAAGGTCTGTTTCGATCAATTCCAGCTGTGGCCGCACCGTACAGGAACGCCGACTACGGTGGTGGTAGGCGTGGTTGTGAGTGATCCGGATACGGGTCTGAGGAAGGTCATCGAGTTTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

216

Amino Acids

24.61

Weight (kDa)

10.14

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025343)

Species Orthologous Gene IDs
rosa_laevigata RLG00000024644 RLG00000035389
rosa_multiflora Rmu_sc0006050.1_g000007

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 492
AccB1I GGYRCC 1 cut(s) 492
AccB7I CCANNNNNTGG 1 cut(s) 483
AccBSI CCGCTC 1 cut(s) 354
AccI GTMKAC 1 cut(s) 348
AccII CGCG 1 cut(s) 321
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 8 cut(s) 167, 232, 340, 352, 393, 490, 498, 557
AclWI GGATC 3 cut(s) 154, 207, 602
AcoI YGGCCR 1 cut(s) 554
AfaI GTAC 5 cut(s) 73, 443, 494, 512, 565
AfiI CCNNNNNNNGG 6 cut(s) 312, 483, 502, 503, 507, 617
AflIII ACRYGT 1 cut(s) 319
AjiI CACGTC 1 cut(s) 409
AjnI CCWGG 1 cut(s) 500
AjuI GAANNNNNNNTTGG 4 cut(s) 209, 241, 254, 286
AluBI AGCT 1 cut(s) 550
AluI AGCT 1 cut(s) 550
Alw26I GTCTC 1 cut(s) 485
AlwI GGATC 3 cut(s) 154, 207, 602
AlwNI CAGNNNCTG 1 cut(s) 483
Ama87I CYCGRG 1 cut(s) 201
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 2 cut(s) 389, 554
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
Asp718I GGTACC 1 cut(s) 492
AspS9I GGNCC 1 cut(s) 390
AsuC2I CCSGG 1 cut(s) 388
AsuHPI GGTGA 2 cut(s) 220, 274
AvaI CYCGRG 1 cut(s) 201
BanI GGYRCC 1 cut(s) 492
BbsI GAAGAC 2 cut(s) 19, 37
BccI CCATC 1 cut(s) 418
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 1 cut(s) 502
BciVI GTATCC 1 cut(s) 607
BcnI CCSGG 1 cut(s) 388
BcoDI GTCTC 1 cut(s) 485
BfuI GTATCC 1 cut(s) 607
BisI GCNGC 2 cut(s) 168, 557
BlsI GCNGC 2 cut(s) 169, 558
Bme1390I CCNGG 2 cut(s) 388, 502
BmeT110I CYCGRG 1 cut(s) 201
BmgBI CACGTC 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 390
BmiI GGNNCC 1 cut(s) 494
BmrFI CCNGG 2 cut(s) 388, 502
BpiI GAAGAC 2 cut(s) 19, 37
BpuMI CCSGG 1 cut(s) 388
BsaBI GATNNNNATC 1 cut(s) 217
BsaJI CCNNGG 1 cut(s) 501
BsaWI WCCGGW 1 cut(s) 610
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 6 cut(s) 312, 483, 502, 503, 507, 617
Bse1I ACTGG 1 cut(s) 477
Bse8I GATNNNNATC 1 cut(s) 217
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 1 cut(s) 502
BseDI CCNNGG 1 cut(s) 501
BseGI GGATG 3 cut(s) 33, 368, 429
BseJI GATNNNNATC 1 cut(s) 217
BseLI CCNNNNNNNGG 6 cut(s) 312, 483, 502, 503, 507, 617
BseMII CTCAG 1 cut(s) 614
BseNI ACTGG 1 cut(s) 477
BseRI GAGGAG 4 cut(s) 78, 81, 101, 110
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 2 cut(s) 391, 556
BshNI GGYRCC 1 cut(s) 492
BsiHKCI CYCGRG 1 cut(s) 201
BsiSI CCGG 2 cut(s) 388, 611
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 6 cut(s) 312, 483, 502, 503, 507, 617
BsmAI GTCTC 1 cut(s) 485
BsmFI GGGAC 1 cut(s) 370
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 2 cut(s) 391, 556
BsoBI CYCGRG 1 cut(s) 201
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 5 cut(s) 9, 159, 212, 538, 607
BspACI CCGC 8 cut(s) 167, 232, 340, 352, 393, 490, 498, 557
BspANI GGCC 2 cut(s) 391, 556
BspCNI CTCAG 1 cut(s) 615
BspEI TCCGGA 1 cut(s) 610
BspFNI CGCG 1 cut(s) 321
BspLI GGNNCC 1 cut(s) 494
BspPI GGATC 3 cut(s) 154, 207, 602
BspT107I GGYRCC 1 cut(s) 492
BsrBI CCGCTC 1 cut(s) 354
BsrI ACTGG 1 cut(s) 477
BssECI CCNNGG 1 cut(s) 501
BssMI GATC 5 cut(s) 9, 159, 212, 538, 607
Bst2UI CCWGG 1 cut(s) 502
Bst4CI ACNGT 3 cut(s) 449, 563, 583
Bst6I CTCTTC 4 cut(s) 44, 119, 281, 365
BstC8I GCNNGC 1 cut(s) 393
BstDEI CTNAG 1 cut(s) 623
BstF5I GGATG 3 cut(s) 33, 368, 429
BstFNI CGCG 1 cut(s) 321
BstKTI GATC 5 cut(s) 12, 162, 215, 541, 610
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 5 cut(s) 9, 159, 212, 538, 607
BstMWI GCNNNNNNNGC 2 cut(s) 197, 556
BstNI CCWGG 1 cut(s) 502
BstSCI CCNGG 2 cut(s) 386, 500
BstUI CGCG 1 cut(s) 321
BstV2I GAAGAC 2 cut(s) 19, 37
BsuI GTATCC 1 cut(s) 607
BsuRI GGCC 2 cut(s) 391, 556
BtrI CACGTC 1 cut(s) 409
BtsCI GGATG 3 cut(s) 33, 368, 429
Cac8I GCNNGC 1 cut(s) 393
CaiI CAGNNNCTG 1 cut(s) 483
Cfr13I GGNCC 1 cut(s) 390
Csp6I GTAC 5 cut(s) 72, 442, 493, 511, 564
CviAII CATG 1 cut(s) 517
CviJI RGCY 7 cut(s) 170, 191, 206, 259, 391, 550, 556
CviKI_1 RGCY 7 cut(s) 170, 191, 206, 259, 391, 550, 556
CviQI GTAC 5 cut(s) 72, 442, 493, 511, 564
DdeI CTNAG 1 cut(s) 623
DpnI GATC 5 cut(s) 11, 161, 214, 540, 609
DpnII GATC 5 cut(s) 9, 159, 212, 538, 607
EaeI YGGCCR 1 cut(s) 554
Eam1104I CTCTTC 4 cut(s) 44, 119, 281, 365
EarI CTCTTC 4 cut(s) 44, 119, 281, 365
Eco88I CYCGRG 1 cut(s) 201
EcoRII CCWGG 1 cut(s) 500
FaeI CATG 1 cut(s) 520
FaiI YATR 2 cut(s) 345, 518
FaqI GGGAC 1 cut(s) 370
FatI CATG 1 cut(s) 516
FauI CCCGC 3 cut(s) 239, 400, 505
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 2 cut(s) 168, 557
FokI GGATG 3 cut(s) 20, 355, 436
Fsp4HI GCNGC 2 cut(s) 168, 557
GluI GCNGC 2 cut(s) 168, 557
HaeIII GGCC 2 cut(s) 391, 556
HapII CCGG 2 cut(s) 388, 611
Hin1II CATG 1 cut(s) 520
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HinfI GANTC 4 cut(s) 245, 401, 418, 429
HpaII CCGG 2 cut(s) 388, 611
HphI GGTGA 2 cut(s) 220, 274
Hpy166II GTNNAC 2 cut(s) 349, 417
Hpy188I TCNGA 5 cut(s) 212, 292, 406, 423, 624
Hpy188III TCNNGA 3 cut(s) 157, 484, 611
Hpy8I GTNNAC 2 cut(s) 349, 417
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 6 cut(s) 89, 280, 346, 501, 520, 622
HpyCH4III ACNGT 3 cut(s) 449, 563, 583
HpyCH4IV ACGT 1 cut(s) 408
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 556
HpyF3I CTNAG 1 cut(s) 623
HpySE526I ACGT 1 cut(s) 408
Hsp92II CATG 1 cut(s) 520
Kpn2I TCCGGA 1 cut(s) 610
KpnI GGTACC 1 cut(s) 496
Kzo9I GATC 5 cut(s) 9, 159, 212, 538, 607
MaeII ACGT 1 cut(s) 408
MalI GATC 5 cut(s) 11, 161, 214, 540, 609
MbiI CCGCTC 1 cut(s) 354
MboI GATC 5 cut(s) 9, 159, 212, 538, 607
MboII GAAGA 9 cut(s) 4, 7, 10, 19, 31, 37, 106, 268, 352
MluCI AATT 2 cut(s) 470, 542
MluI ACGCGT 1 cut(s) 319
MlyI GAGTC 3 cut(s) 410, 412, 438
MmeI TCCRAC 1 cut(s) 315
MroI TCCGGA 1 cut(s) 610
MseI TTAA 1 cut(s) 473
MspA1I CMGCKG 1 cut(s) 550
MspI CCGG 2 cut(s) 388, 611
MspR9I CCNGG 2 cut(s) 388, 502
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 1 cut(s) 502
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 2 cut(s) 197, 556
NciI CCSGG 1 cut(s) 388
NdeII GATC 5 cut(s) 9, 159, 212, 538, 607
NlaIII CATG 1 cut(s) 520
NlaIV GGNNCC 1 cut(s) 494
PctI GAATGC 1 cut(s) 224
PfeI GAWTC 1 cut(s) 245
PflMI CCANNNNNTGG 1 cut(s) 483
PkrI GCNGC 2 cut(s) 169, 558
PleI GAGTC 3 cut(s) 409, 412, 437
PpsI GAGTC 3 cut(s) 409, 412, 437
Psp6I CCWGG 1 cut(s) 500
PspGI CCWGG 1 cut(s) 500
PspN4I GGNNCC 1 cut(s) 494
PspPI GGNCC 1 cut(s) 390
PsrI GAACNNNNNNTAC 2 cut(s) 562, 594
PstNI CAGNNNCTG 1 cut(s) 483
PvuII CAGCTG 1 cut(s) 550
RsaI GTAC 5 cut(s) 73, 443, 494, 512, 565
RsaNI GTAC 5 cut(s) 72, 442, 493, 511, 564
SaqAI TTAA 1 cut(s) 473
SatI GCNGC 2 cut(s) 168, 557
Sau3AI GATC 5 cut(s) 9, 159, 212, 538, 607
Sau96I GGNCC 1 cut(s) 390
SchI GAGTC 3 cut(s) 410, 412, 438
ScrFI CCNGG 2 cut(s) 388, 502
SetI ASST 7 cut(s) 297, 411, 438, 512, 531, 552, 633
Sse9I AATT 2 cut(s) 470, 542
SsiI CCGC 8 cut(s) 167, 232, 340, 352, 393, 490, 498, 557
StyD4I CCNGG 2 cut(s) 386, 500
TaaI ACNGT 3 cut(s) 449, 563, 583
TaiI ACGT 1 cut(s) 411
TaqI TCGA 8 cut(s) 158, 180, 243, 378, 456, 524, 537, 636
TasI AATT 2 cut(s) 470, 542
TatI WGTACW 1 cut(s) 441
TauI GCSGC 2 cut(s) 170, 559
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TspDTI ATGAA 1 cut(s) 189
Van91I CCANNNNNTGG 1 cut(s) 483
XmiI GTMKAC 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.