RLG00000035389

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0025343
Physical Location & Seq
Reverse (-)
66162396 .. 66162848
453 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035389

Sequence Viewer

Length: 453 bp
ATGCCCACGATCTTCTTCTTCCTCACCAACTCCGACCTCGACTTCGCCCCCCTCTGGCAATGCGTTCCTCTCCACTCCTTCCGCTACGTCTACCGCTCCCTCTTCGCCTCCTCCACTTTCAACAAGTCCCGGCCCACCACCGAGTCCGACGCGGAGTTGACTCAGATAGGAGTCAAGGTCAAGTACAAGAGCTTTATCGAAATCCTTTCCAAATGGACCAGTCTCTTGAAGCGGTACCTCGCCAGGGGAAGGTACGCCATGATGCCGGAGGTCCGTTTCGATCAGTCCCGGGTCGGGTTGCAGTTCTTTTTGCTCATGCACCATCATGCATTGGTGGTTTTGAAGGATCAGCAACTGTGGCCACACCGTACAGGAATGCCGACTATGGTGGGGGTGGGCGTGGTTGTGAGTGATCCGGATACGGGTTTGAGGAAGTTTGTCGGGTTTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

151

Amino Acids

17.33

Weight (kDa)

9.81

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025343)

Species Orthologous Gene IDs
rosa_laevigata RLG00000024644 RLG00000035389
rosa_multiflora Rmu_sc0006050.1_g000007

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 234
AccB1I GGYRCC 1 cut(s) 234
AccBSI CCGCTC 1 cut(s) 96
AccI GTMKAC 1 cut(s) 90
AccII CGCG 1 cut(s) 152
AccIII TCCGGA 1 cut(s) 415
AciI CCGC 4 cut(s) 82, 94, 152, 232
AclWI GGATC 2 cut(s) 354, 407
AcoI YGGCCR 1 cut(s) 359
AfaI GTAC 4 cut(s) 185, 236, 254, 370
AfiI CCNNNNNNNGG 5 cut(s) 54, 244, 249, 294, 422
AgsI TTSAA 3 cut(s) 121, 229, 343
AjnI CCWGG 1 cut(s) 242
AluBI AGCT 1 cut(s) 192
AluI AGCT 1 cut(s) 192
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 2 cut(s) 354, 407
AlwNI CAGNNNCTG 1 cut(s) 355
Ama87I CYCGRG 1 cut(s) 288
Aor13HI TCCGGA 1 cut(s) 415
AoxI GGCC 2 cut(s) 131, 359
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
Asp718I GGTACC 1 cut(s) 234
AspS9I GGNCC 3 cut(s) 132, 216, 271
AsuC2I CCSGG 3 cut(s) 130, 289, 290
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 288
AvaII GGWCC 2 cut(s) 216, 271
BalI TGGCCA 1 cut(s) 361
BanI GGYRCC 1 cut(s) 234
BccI CCATC 1 cut(s) 330
BciT130I CCWGG 1 cut(s) 244
BciVI GTATCC 1 cut(s) 412
BcnI CCSGG 3 cut(s) 130, 289, 290
BcoDI GTCTC 1 cut(s) 227
BfuI GTATCC 1 cut(s) 412
Bme1390I CCNGG 4 cut(s) 130, 244, 289, 290
Bme18I GGWCC 2 cut(s) 216, 271
BmeT110I CYCGRG 1 cut(s) 288
BmgT120I GGNCC 3 cut(s) 132, 216, 271
BmiI GGNNCC 1 cut(s) 236
BmrFI CCNGG 4 cut(s) 130, 244, 289, 290
BmsI GCATC 1 cut(s) 252
BpuMI CCSGG 3 cut(s) 130, 289, 290
BsaJI CCNNGG 2 cut(s) 243, 288
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 5 cut(s) 54, 244, 249, 294, 422
Bse1I ACTGG 1 cut(s) 219
Bse3DI GCAATG 1 cut(s) 65
BseAI TCCGGA 1 cut(s) 415
BseBI CCWGG 1 cut(s) 244
BseDI CCNNGG 2 cut(s) 243, 288
BseLI CCNNNNNNNGG 5 cut(s) 54, 244, 249, 294, 422
BseMI GCAATG 1 cut(s) 65
BseMII CTCAG 1 cut(s) 176
BseNI ACTGG 1 cut(s) 219
BseRI GAGGAG 1 cut(s) 100
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 2 cut(s) 133, 361
BshNI GGYRCC 1 cut(s) 234
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 4 cut(s) 130, 266, 289, 416
BslFI GGGAC 2 cut(s) 112, 271
BslI CCNNNNNNNGG 5 cut(s) 54, 244, 249, 294, 422
BsmAI GTCTC 1 cut(s) 227
BsmFI GGGAC 2 cut(s) 112, 271
BsmI GAATGC 1 cut(s) 381
BsnI GGCC 2 cut(s) 133, 361
BsoBI CYCGRG 1 cut(s) 288
Bsp13I TCCGGA 1 cut(s) 415
Bsp143I GATC 4 cut(s) 9, 280, 346, 412
BspACI CCGC 4 cut(s) 82, 94, 152, 232
BspANI GGCC 2 cut(s) 133, 361
BspCNI CTCAG 1 cut(s) 175
BspEI TCCGGA 1 cut(s) 415
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 1 cut(s) 236
BspPI GGATC 2 cut(s) 354, 407
BspT107I GGYRCC 1 cut(s) 234
BsrBI CCGCTC 1 cut(s) 96
BsrDI GCAATG 1 cut(s) 65
BsrI ACTGG 1 cut(s) 219
BssECI CCNNGG 2 cut(s) 243, 288
BssMI GATC 4 cut(s) 9, 280, 346, 412
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 2 cut(s) 357, 368
Bst6I CTCTTC 1 cut(s) 107
BstDEI CTNAG 1 cut(s) 162
BstFNI CGCG 1 cut(s) 152
BstKTI GATC 4 cut(s) 12, 283, 349, 415
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 4 cut(s) 9, 280, 346, 412
BstMWI GCNNNNNNNGC 1 cut(s) 358
BstNI CCWGG 1 cut(s) 244
BstSCI CCNGG 4 cut(s) 128, 242, 287, 288
BstUI CGCG 1 cut(s) 152
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 2 cut(s) 133, 361
CaiI CAGNNNCTG 1 cut(s) 355
Cfr13I GGNCC 3 cut(s) 132, 216, 271
Cfr9I CCCGGG 1 cut(s) 288
CseI GACGC 1 cut(s) 158
Csp6I GTAC 4 cut(s) 184, 235, 253, 369
CviAII CATG 3 cut(s) 259, 316, 326
CviJI RGCY 3 cut(s) 133, 192, 361
CviKI_1 RGCY 3 cut(s) 133, 192, 361
CviQI GTAC 4 cut(s) 184, 235, 253, 369
DdeI CTNAG 1 cut(s) 162
DpnI GATC 4 cut(s) 11, 282, 348, 414
DpnII GATC 4 cut(s) 9, 280, 346, 412
EaeI YGGCCR 1 cut(s) 359
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
Eco47I GGWCC 2 cut(s) 216, 271
Eco88I CYCGRG 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 242
EcoT22I ATGCAT 1 cut(s) 331
FaeI CATG 3 cut(s) 262, 319, 329
FaiI YATR 4 cut(s) 260, 317, 327, 386
FaqI GGGAC 2 cut(s) 112, 271
FatI CATG 3 cut(s) 258, 315, 325
FblI GTMKAC 1 cut(s) 90
HaeIII GGCC 2 cut(s) 133, 361
HapII CCGG 4 cut(s) 130, 266, 289, 416
HgaI GACGC 1 cut(s) 158
Hin1II CATG 3 cut(s) 262, 319, 329
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HinfI GANTC 3 cut(s) 143, 160, 171
HpaII CCGG 4 cut(s) 130, 266, 289, 416
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 91, 159
Hpy188I TCNGA 3 cut(s) 34, 148, 165
Hpy188III TCNNGA 2 cut(s) 226, 416
Hpy8I GTNNAC 2 cut(s) 91, 159
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 3 cut(s) 88, 243, 337
HpyCH4III ACNGT 2 cut(s) 357, 368
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 3 cut(s) 301, 319, 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 358
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 1 cut(s) 87
Hsp92II CATG 3 cut(s) 262, 319, 329
Kpn2I TCCGGA 1 cut(s) 415
KpnI GGTACC 1 cut(s) 238
Kzo9I GATC 4 cut(s) 9, 280, 346, 412
LmnI GCTCC 1 cut(s) 101
LpnPI CCDG 9 cut(s) 40, 143, 229, 232, 256, 279, 302, 357, 429
LweI GCATC 1 cut(s) 252
MaeII ACGT 1 cut(s) 87
MalI GATC 4 cut(s) 11, 282, 348, 414
MbiI CCGCTC 1 cut(s) 96
MboI GATC 4 cut(s) 9, 280, 346, 412
MboII GAAGA 4 cut(s) 4, 7, 10, 94
MlsI TGGCCA 1 cut(s) 361
MluNI TGGCCA 1 cut(s) 361
MlyI GAGTC 3 cut(s) 152, 154, 180
MmeI TCCRAC 2 cut(s) 57, 171
Mox20I TGGCCA 1 cut(s) 361
Mph1103I ATGCAT 1 cut(s) 331
MroI TCCGGA 1 cut(s) 415
MscI TGGCCA 1 cut(s) 361
MslI CAYNNNNRTG 1 cut(s) 324
Msp20I TGGCCA 1 cut(s) 361
MspI CCGG 4 cut(s) 130, 266, 289, 416
MspR9I CCNGG 4 cut(s) 130, 244, 289, 290
Mva1269I GAATGC 1 cut(s) 381
MvaI CCWGG 1 cut(s) 244
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 358
NciI CCSGG 3 cut(s) 130, 289, 290
NdeII GATC 4 cut(s) 9, 280, 346, 412
NlaIII CATG 3 cut(s) 262, 319, 329
NlaIV GGNNCC 1 cut(s) 236
NsiI ATGCAT 1 cut(s) 331
PctI GAATGC 1 cut(s) 381
PleI GAGTC 3 cut(s) 151, 154, 179
PpsI GAGTC 3 cut(s) 151, 154, 179
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 236
PspPI GGNCC 3 cut(s) 132, 216, 271
PstNI CAGNNNCTG 1 cut(s) 355
RsaI GTAC 4 cut(s) 185, 236, 254, 370
RsaNI GTAC 4 cut(s) 184, 235, 253, 369
RseI CAYNNNNRTG 1 cut(s) 324
Sau3AI GATC 4 cut(s) 9, 280, 346, 412
Sau96I GGNCC 3 cut(s) 132, 216, 271
SchI GAGTC 3 cut(s) 152, 154, 180
ScrFI CCNGG 4 cut(s) 130, 244, 289, 290
SetI ASST 7 cut(s) 39, 90, 180, 194, 240, 254, 273
SfaNI GCATC 1 cut(s) 252
SinI GGWCC 2 cut(s) 216, 271
SmaI CCCGGG 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 324
SsiI CCGC 4 cut(s) 82, 94, 152, 232
StyD4I CCNGG 4 cut(s) 128, 242, 287, 288
TaaI ACNGT 2 cut(s) 357, 368
TaiI ACGT 1 cut(s) 90
TaqI TCGA 3 cut(s) 39, 198, 279
TatI WGTACW 1 cut(s) 183
TspGWI ACGGA 1 cut(s) 263
TspMI CCCGGG 1 cut(s) 288
VpaK11BI GGWCC 2 cut(s) 216, 271
XmaI CCCGGG 1 cut(s) 288
XmiI GTMKAC 1 cut(s) 90
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.