RLG00000025588

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017030
Physical Location & Seq
Forward (+)
46947807 .. 46948352
546 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025588

Sequence Viewer

Length: 546 bp
ATGGCGGTCATCGACTCTATCCCACAATCAACCCCAGGCCCTCTCCTTTCATCGTCTGCTTCTTGTTCTTCGACGTCAGATCTCGAAGATGGAATTCAGGAAGTCATCTATTTCGTTAAGAGTTTTCCTATAATAATAGAAGCAAATGTGTATAGAGATTGGCCGATTGTGAAATCTGCTTTGGGGGAGCATGATATTAATTGCCATTTGGATATGGTTAACAGTCGCATGACATTCTCAACAACCAGAACCAAGGAACCAGAAATTGCGCGCAAGGCGAGGCATCTTCTCCAACTTTTGTCACGGAATGTTCCGGCATCTTCGGCACTAAACATAATAAAAGTGCCCAGTTGCGAATGCGAAATCATCTTCACTGGTCTTAGTAAAGTCAACAATAGTTATTTAAAGGAACTGGAAGGGCAGACGTCATGCCAAATTTTTGCTCGTGATGGGTATGTTCGCGATGGGGTTGTTTTCGATGGCTTTGTTATTGCCATTGGTCCTTTGGAGGGACTAACAATAGTTAGAAAGGCTGTGGAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

19.87

Weight (kDa)

5.39

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_KRR1_1st PF17903 41 - 113 4.9e-09 Krr1 KH1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 77, 428
AccII CGCG 2 cut(s) 271, 462
AciI CCGC 1 cut(s) 5
AcoI YGGCCR 1 cut(s) 161
AcsI RAATTY 2 cut(s) 93, 435
AcyI GRCGYC 2 cut(s) 74, 425
AfiI CCNNNNNNNGG 1 cut(s) 509
AjnI CCWGG 1 cut(s) 34
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AoxI GGCC 2 cut(s) 37, 161
ApoI RAATTY 2 cut(s) 93, 435
AseI ATTAAT 1 cut(s) 198
AspLEI GCGC 2 cut(s) 271, 273
AspS9I GGNCC 2 cut(s) 38, 500
AvaII GGWCC 1 cut(s) 500
BaeGI GKGCMC 1 cut(s) 348
BauI CACGAG 1 cut(s) 444
BccI CCATC 4 cut(s) 83, 443, 458, 473
BciT130I CCWGG 1 cut(s) 36
BglII AGATCT 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 36
Bme18I GGWCC 1 cut(s) 500
BmgT120I GGNCC 2 cut(s) 38, 500
BmiI GGNNCC 1 cut(s) 258
BmrFI CCNGG 1 cut(s) 36
BmrI ACTGGG 1 cut(s) 342
BmsI GCATC 2 cut(s) 292, 326
BmuI ACTGGG 1 cut(s) 342
BsaHI GRCGYC 2 cut(s) 74, 425
BsaJI CCNNGG 2 cut(s) 34, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse1I ACTGG 3 cut(s) 348, 379, 417
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 2 cut(s) 34, 252
BseLI CCNNNNNNNGG 1 cut(s) 509
BseNI ACTGG 3 cut(s) 348, 379, 417
BsePI GCGCGC 1 cut(s) 269
BseSI GKGCMC 1 cut(s) 348
Bsh1236I CGCG 2 cut(s) 271, 462
BshFI GGCC 2 cut(s) 39, 163
BsiSI CCGG 1 cut(s) 314
BslFI GGGAC 1 cut(s) 525
BslI CCNNNNNNNGG 1 cut(s) 509
BsmFI GGGAC 1 cut(s) 525
BsmI GAATGC 1 cut(s) 362
BsnI GGCC 2 cut(s) 39, 163
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 1 cut(s) 79
Bsp68I TCGCGA 1 cut(s) 462
BspACI CCGC 1 cut(s) 5
BspANI GGCC 2 cut(s) 39, 163
BspFNI CGCG 2 cut(s) 271, 462
BspLI GGNNCC 1 cut(s) 258
BsrI ACTGG 3 cut(s) 348, 379, 417
BssECI CCNNGG 2 cut(s) 34, 252
BssHII GCGCGC 1 cut(s) 269
BssMI GATC 1 cut(s) 79
BssNI GRCGYC 2 cut(s) 74, 425
BssSI CACGAG 1 cut(s) 444
BssT1I CCWWGG 1 cut(s) 252
Bst2BI CACGAG 1 cut(s) 444
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 1 cut(s) 224
BstACI GRCGYC 2 cut(s) 74, 425
BstC8I GCNNGC 1 cut(s) 271
BstDEI CTNAG 1 cut(s) 380
BstFNI CGCG 2 cut(s) 271, 462
BstHHI GCGC 2 cut(s) 271, 273
BstKTI GATC 1 cut(s) 82
BstMBI GATC 1 cut(s) 79
BstMWI GCNNNNNNNGC 2 cut(s) 275, 323
BstNI CCWGG 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 34
BstSLI GKGCMC 1 cut(s) 348
BstUI CGCG 2 cut(s) 271, 462
BstX2I RGATCY 1 cut(s) 79
BstYI RGATCY 1 cut(s) 79
BsuRI GGCC 2 cut(s) 39, 163
BtgZI GCGATG 1 cut(s) 477
BtsIMutI CAGTG 1 cut(s) 372
BtuMI TCGCGA 1 cut(s) 462
Cac8I GCNNGC 1 cut(s) 271
CfoI GCGC 2 cut(s) 271, 273
Cfr13I GGNCC 2 cut(s) 38, 500
CviAII CATG 3 cut(s) 191, 229, 429
CviJI RGCY 4 cut(s) 39, 163, 483, 533
CviKI_1 RGCY 4 cut(s) 39, 163, 483, 533
DdeI CTNAG 1 cut(s) 380
DpnI GATC 1 cut(s) 81
DpnII GATC 1 cut(s) 79
DraI TTTAAA 1 cut(s) 405
EaeI YGGCCR 1 cut(s) 161
Eco130I CCWWGG 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 500
EcoO109I RGGNCCY 1 cut(s) 38
EcoRI GAATTC 1 cut(s) 93
EcoRII CCWGG 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 3 cut(s) 194, 232, 432
FaiI YATR 8 cut(s) 131, 153, 192, 215, 230, 335, 430, 456
FaqI GGGAC 1 cut(s) 525
FatI CATG 3 cut(s) 190, 228, 428
GlaI GCGC 2 cut(s) 270, 272
HaeIII GGCC 2 cut(s) 39, 163
HapII CCGG 1 cut(s) 314
HhaI GCGC 2 cut(s) 271, 273
Hin1I GRCGYC 2 cut(s) 74, 425
Hin1II CATG 3 cut(s) 194, 232, 432
Hin6I GCGC 2 cut(s) 269, 271
HinP1I GCGC 2 cut(s) 269, 271
HincII GTYRAC 2 cut(s) 220, 391
HindII GTYRAC 2 cut(s) 220, 391
HinfI GANTC 1 cut(s) 14
HpaI GTTAAC 1 cut(s) 220
HpaII CCGG 1 cut(s) 314
Hpy166II GTNNAC 2 cut(s) 220, 391
Hpy188I TCNGA 1 cut(s) 79
Hpy188III TCNNGA 4 cut(s) 83, 98, 446, 461
Hpy8I GTNNAC 2 cut(s) 220, 391
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 1 cut(s) 410
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 2 cut(s) 74, 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 323
HpyF3I CTNAG 1 cut(s) 380
HpySE526I ACGT 2 cut(s) 74, 425
Hsp92I GRCGYC 2 cut(s) 74, 425
Hsp92II CATG 3 cut(s) 194, 232, 432
HspAI GCGC 2 cut(s) 269, 271
KspAI GTTAAC 1 cut(s) 220
Kzo9I GATC 1 cut(s) 79
LmnI GCTCC 1 cut(s) 187
LpnPI CCDG 9 cut(s) 21, 48, 83, 259, 273, 327, 360, 361, 398
LweI GCATC 2 cut(s) 292, 326
MaeII ACGT 2 cut(s) 74, 425
MaeIII GTNAC 1 cut(s) 300
MalI GATC 1 cut(s) 81
MboI GATC 1 cut(s) 79
MboII GAAGA 5 cut(s) 60, 98, 278, 312, 361
MflI RGATCY 1 cut(s) 79
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 4 cut(s) 93, 199, 264, 435
MlyI GAGTC 1 cut(s) 8
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 3 cut(s) 51, 273, 502
MseI TTAA 4 cut(s) 117, 198, 219, 404
MspI CCGG 1 cut(s) 314
MspR9I CCNGG 1 cut(s) 36
Mva1269I GAATGC 1 cut(s) 362
MvaI CCWGG 1 cut(s) 36
MvnI CGCG 2 cut(s) 271, 462
MwoI GCNNNNNNNGC 2 cut(s) 275, 323
NdeII GATC 1 cut(s) 79
NlaIII CATG 3 cut(s) 194, 232, 432
NlaIV GGNNCC 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 300
NruI TCGCGA 1 cut(s) 462
PauI GCGCGC 1 cut(s) 269
PctI GAATGC 1 cut(s) 362
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 2 cut(s) 38, 500
PsuI RGATCY 1 cut(s) 79
PteI GCGCGC 1 cut(s) 269
RruI TCGCGA 1 cut(s) 462
SaqAI TTAA 4 cut(s) 117, 198, 219, 404
Sau3AI GATC 1 cut(s) 79
Sau96I GGNCC 2 cut(s) 38, 500
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 1 cut(s) 348
SetI ASST 2 cut(s) 77, 428
SfaNI GCATC 2 cut(s) 292, 326
SinI GGWCC 1 cut(s) 500
Sse9I AATT 4 cut(s) 93, 199, 264, 435
SsiI CCGC 1 cut(s) 5
StyD4I CCNGG 1 cut(s) 34
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 2 cut(s) 77, 428
TaqI TCGA 4 cut(s) 12, 71, 84, 477
TasI AATT 4 cut(s) 93, 199, 264, 435
Tru1I TTAA 4 cut(s) 117, 198, 219, 404
Tru9I TTAA 4 cut(s) 117, 198, 219, 404
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 300
TspDTI ATGAA 1 cut(s) 39
TspGWI ACGGA 1 cut(s) 319
TspRI CASTG 1 cut(s) 379
VpaK11BI GGWCC 1 cut(s) 500
VspI ATTAAT 1 cut(s) 198
XapI RAATTY 2 cut(s) 93, 435
XcmI CCANNNNNNNNNTGG 1 cut(s) 502
ZraI GACGTC 2 cut(s) 75, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.