RLG00000025893

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0030821
Physical Location & Seq
Forward (+)
49038127 .. 49038817
691 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025893

Sequence Viewer

Length: 390 bp
ATGCCGAATATTTTTTATAGCCATCACCACTGGTACTCATCCATTATTGAGTCCTTGAAGCCTGATAATCCTGAGTATTCAGATGTGGGATTTGGCCAGAGAGCGAAATTAAGCTTCAAAGACGATGGGATGCCCGCCAAATTTCCACTTAGGTGGAAGGCTACATGCGCGGTTGGACATGATTTCAACAATTCCATGTGTTATGTGGACACATTGGAGCACGGGACACACACGGTTGCCAGGAACTATGTACCCGGTGCTTCTTTTTTTGGTTATGCTAAAGGAATAGCCAGAGGTGTTGCACTTCGTGCTAGACTAGCCGTCTACAAGTCACATGGGAAGAGGGGCATTACGTGTACTATGTCTTGTGGGGATGGACCAAGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

130

Amino Acids

14.29

Weight (kDa)

9.12

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030821)

Species Orthologous Gene IDs
rosa_laevigata RLG00000025893
rosa_multiflora Rmu_sc0003116.1_g000047

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 324
AccII CGCG 1 cut(s) 170
AciI CCGC 2 cut(s) 135, 170
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 1 cut(s) 140
AdeI CACNNNGTG 1 cut(s) 308
AfaI GTAC 3 cut(s) 35, 252, 358
AflIII ACRYGT 1 cut(s) 353
AgsI TTSAA 3 cut(s) 58, 118, 187
AhdI GACNNNNNGTC 1 cut(s) 320
AjnI CCWGG 1 cut(s) 239
AleI CACNNNNGTG 1 cut(s) 151
AluBI AGCT 1 cut(s) 114
AluI AGCT 1 cut(s) 114
Alw21I GWGCWC 1 cut(s) 222
AoxI GGCC 1 cut(s) 94
ApoI RAATTY 1 cut(s) 140
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 1 cut(s) 377
AsuC2I CCSGG 1 cut(s) 255
AsuHPI GGTGA 1 cut(s) 17
AvaII GGWCC 1 cut(s) 377
BalI TGGCCA 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 222
BccI CCATC 3 cut(s) 30, 119, 368
BceAI ACGGC 1 cut(s) 305
BciT130I CCWGG 1 cut(s) 241
BcnI CCSGG 1 cut(s) 255
BfaI CTAG 2 cut(s) 312, 317
Bme1390I CCNGG 2 cut(s) 241, 255
Bme18I GGWCC 1 cut(s) 377
BmeRI GACNNNNNGTC 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 377
BmrFI CCNGG 2 cut(s) 241, 255
BmsI GCATC 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 255
BsaAI YACGTR 1 cut(s) 354
Bse1I ACTGG 1 cut(s) 35
BseBI CCWGG 1 cut(s) 241
BseGI GGATG 3 cut(s) 38, 135, 379
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 35
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 222
BsiSI CCGG 1 cut(s) 255
BslFI GGGAC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 1 cut(s) 96
Bsp1286I GDGCHC 1 cut(s) 222
BspACI CCGC 2 cut(s) 135, 170
BspANI GGCC 1 cut(s) 96
BspCNI CTCAG 1 cut(s) 64
BspFNI CGCG 1 cut(s) 170
BsrI ACTGG 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 1 cut(s) 235
Bst6I CTCTTC 1 cut(s) 335
BstAPI GCANNNNNTGC 1 cut(s) 308
BstBAI YACGTR 1 cut(s) 354
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 2 cut(s) 72, 149
BstF5I GGATG 3 cut(s) 38, 135, 379
BstFNI CGCG 1 cut(s) 170
BstHHI GCGC 1 cut(s) 170
BstMWI GCNNNNNNNGC 3 cut(s) 167, 308, 317
BstNI CCWGG 1 cut(s) 241
BstNSI RCATGY 1 cut(s) 168
BstSCI CCNGG 2 cut(s) 239, 253
BstUI CGCG 1 cut(s) 170
BstXI CCANNNNNNTGG 1 cut(s) 153
BsuRI GGCC 1 cut(s) 96
BtsCI GGATG 3 cut(s) 38, 135, 379
BtsIMutI CAGTG 1 cut(s) 28
Cac8I GCNNGC 1 cut(s) 135
CfoI GCGC 1 cut(s) 170
Cfr13I GGNCC 1 cut(s) 377
Csp6I GTAC 3 cut(s) 34, 251, 357
CviAII CATG 4 cut(s) 165, 179, 196, 335
CviJI RGCY 7 cut(s) 21, 61, 96, 114, 161, 290, 320
CviKI_1 RGCY 7 cut(s) 21, 61, 96, 114, 161, 290, 320
CviQI GTAC 3 cut(s) 34, 251, 357
DdeI CTNAG 2 cut(s) 72, 149
DraIII CACNNNGTG 1 cut(s) 308
DriI GACNNNNNGTC 1 cut(s) 320
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 335
Eam1105I GACNNNNNGTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 239
FaeI CATG 4 cut(s) 168, 182, 199, 338
FaiI YATR 9 cut(s) 18, 166, 180, 197, 204, 249, 276, 336, 362
FaqI GGGAC 1 cut(s) 238
FatI CATG 4 cut(s) 164, 178, 195, 334
FauI CCCGC 1 cut(s) 142
FblI GTMKAC 1 cut(s) 324
FokI GGATG 3 cut(s) 25, 142, 386
FspBI CTAG 2 cut(s) 312, 317
GlaI GCGC 1 cut(s) 169
HaeIII GGCC 1 cut(s) 96
HapII CCGG 1 cut(s) 255
HhaI GCGC 1 cut(s) 170
Hin1II CATG 4 cut(s) 168, 182, 199, 338
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 1 cut(s) 50
HpaII CCGG 1 cut(s) 255
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 3 cut(s) 208, 325, 357
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 1 cut(s) 71
Hpy8I GTNNAC 3 cut(s) 208, 325, 357
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 308, 317
HpyF3I CTNAG 2 cut(s) 72, 149
HpySE526I ACGT 1 cut(s) 353
Hsp92II CATG 4 cut(s) 168, 182, 199, 338
HspAI GCGC 1 cut(s) 168
LmnI GCTCC 1 cut(s) 217
LpnPI CCDG 8 cut(s) 16, 75, 84, 110, 226, 253, 268, 304
LweI GCATC 1 cut(s) 120
MaeI CTAG 2 cut(s) 312, 317
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 1 cut(s) 330
MboII GAAGA 1 cut(s) 352
MfeI CAATTG 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 222
MlsI TGGCCA 1 cut(s) 96
MluCI AATT 4 cut(s) 107, 140, 190, 385
MluNI TGGCCA 1 cut(s) 96
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 2 cut(s) 287, 336
Mox20I TGGCCA 1 cut(s) 96
MscI TGGCCA 1 cut(s) 96
MseI TTAA 1 cut(s) 110
MslI CAYNNNNRTG 1 cut(s) 151
Msp20I TGGCCA 1 cut(s) 96
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 2 cut(s) 241, 255
MunI CAATTG 1 cut(s) 385
MvaI CCWGG 1 cut(s) 241
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 3 cut(s) 167, 308, 317
NciI CCSGG 1 cut(s) 255
NlaIII CATG 4 cut(s) 168, 182, 199, 338
NmuCI GTSAC 1 cut(s) 330
NspI RCATGY 1 cut(s) 168
OliI CACNNNNGTG 1 cut(s) 151
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
Ppu21I YACGTR 1 cut(s) 354
Psp6I CCWGG 1 cut(s) 239
PspGI CCWGG 1 cut(s) 239
PspPI GGNCC 1 cut(s) 377
RsaI GTAC 3 cut(s) 35, 252, 358
RsaNI GTAC 3 cut(s) 34, 251, 357
RseI CAYNNNNRTG 1 cut(s) 151
SaqAI TTAA 1 cut(s) 110
Sau96I GGNCC 1 cut(s) 377
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 2 cut(s) 241, 255
SduI GDGCHC 1 cut(s) 222
SetI ASST 4 cut(s) 116, 155, 298, 356
SfaNI GCATC 1 cut(s) 120
SinI GGWCC 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 151
Sse9I AATT 4 cut(s) 107, 140, 190, 385
SsiI CCGC 2 cut(s) 135, 170
SspI AATATT 1 cut(s) 10
SspMI CTAG 2 cut(s) 312, 317
StyD4I CCNGG 2 cut(s) 239, 253
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 1 cut(s) 356
TasI AATT 4 cut(s) 107, 140, 190, 385
TatI WGTACW 1 cut(s) 356
Tru1I TTAA 1 cut(s) 110
Tru9I TTAA 1 cut(s) 110
TscAI CASTG 1 cut(s) 35
TseFI GTSAC 1 cut(s) 330
Tsp45I GTSAC 1 cut(s) 330
TspRI CASTG 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 377
XapI RAATTY 1 cut(s) 140
XceI RCATGY 1 cut(s) 168
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 2 cut(s) 312, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.