RLG00000028555

DNA-damage-repair toleration protein DRT111

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0030901
Physical Location & Seq
Forward (+)
24278324 .. 24280050
1727 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028555

Sequence Viewer

Length: 447 bp
ATGGCGCCCCAAACTCTCCACAAGCCTTCTTCGATTTGCGTGCCAATGCAGACGATCCTCAAATCCCAGAACAAGCCCAAACCGTCAAGCTCAGTGATTTCCAAAATCACGGCATCTCTAATGATGCACAACGAGGCGGTGCAGCCGGCGCTGGTTGAAATCACGTCGACGGTGCTGGAGGAGTACGATCCGGCCAGGCCGAACGACCACGTGGAGTATAGGAGGGAGAGGAAGAAGAAGGCGATGGAGGCCAAGGAGAAGAGAGAGCTCGATTGGAGAAGACACGAGGAGAAGAGGATAGAGGGGGAGGAGAGAGAAAGAGAAAAAGAGGGGGATTTCGTTGATTCAAGGTTGAACATTTCTGGTGAGGAAGTGTGGAGGAGGAGGACAGCGATGAGTGGATGGATGTTTGGGACGATGAGCAGAATAGGGCCTGTGTTTGGTTGA

Protein Analysis

149

Amino Acids

17.2

Weight (kDa)

9.07

Isoelectric Point (pI)

81.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030901)

Species Orthologous Gene IDs
rosa_laevigata RLG00000028555
rosa_multiflora Rmu_sc0004208.1_g000014

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 167
AciI CCGC 1 cut(s) 137
AclWI GGATC 2 cut(s) 49, 182
AcoI YGGCCR 1 cut(s) 192
AcvI CACGTG 1 cut(s) 211
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 1 cut(s) 440
AgsI TTSAA 3 cut(s) 158, 348, 355
AjiI CACGTC 1 cut(s) 165
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 2 cut(s) 90, 268
AluI AGCT 2 cut(s) 90, 268
Alw21I GWGCWC 1 cut(s) 270
AlwI GGATC 2 cut(s) 49, 182
AoxI GGCC 4 cut(s) 192, 197, 249, 431
ApeKI GCWGC 1 cut(s) 142
AspLEI GCGC 2 cut(s) 7, 151
AspS9I GGNCC 1 cut(s) 431
AsuHPI GGTGA 1 cut(s) 377
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 270
BauI CACGAG 1 cut(s) 284
BbrPI CACGTG 1 cut(s) 211
BbsI GAAGAC 1 cut(s) 286
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 1 cut(s) 154
BccI CCATC 2 cut(s) 238, 396
BceAI ACGGC 1 cut(s) 126
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 1 cut(s) 196
BfoI RGCGCY 2 cut(s) 8, 152
BisI GCNGC 1 cut(s) 143
BlsI GCNGC 1 cut(s) 144
Bme1390I CCNGG 1 cut(s) 196
BmgBI CACGTC 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 431
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 196
BmsI GCATC 2 cut(s) 114, 122
BpiI GAAGAC 1 cut(s) 286
BpmI CTGGAG 1 cut(s) 197
BsaAI YACGTR 1 cut(s) 211
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 252
Bsc4I CCNNNNNNNGG 1 cut(s) 440
Bse118I RCCGGY 1 cut(s) 145
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 2 cut(s) 407, 411
BseLI CCNNNNNNNGG 1 cut(s) 440
BseMII CTCAG 1 cut(s) 105
BseRI GAGGAG 5 cut(s) 194, 302, 323, 394, 397
BseXI GCAGC 1 cut(s) 154
BsgI GTGCAG 1 cut(s) 161
BshFI GGCC 4 cut(s) 194, 199, 251, 433
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 270
BsiSI CCGG 2 cut(s) 146, 191
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 1 cut(s) 440
BsmFI GGGAC 1 cut(s) 427
BsnI GGCC 4 cut(s) 194, 199, 251, 433
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 2 cut(s) 54, 187
BspACI CCGC 1 cut(s) 137
BspANI GGCC 4 cut(s) 194, 199, 251, 433
BspCNI CTCAG 1 cut(s) 104
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 2 cut(s) 49, 182
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 2 cut(s) 54, 187
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 284
BssT1I CCWWGG 1 cut(s) 252
Bst2BI CACGAG 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 2 cut(s) 84, 172
Bst6I CTCTTC 2 cut(s) 254, 287
BstACI GRCGYC 1 cut(s) 5
BstBAI YACGTR 1 cut(s) 211
BstC8I GCNNGC 2 cut(s) 41, 147
BstDEI CTNAG 1 cut(s) 91
BstF5I GGATG 2 cut(s) 407, 411
BstH2I RGCGCY 2 cut(s) 8, 152
BstHHI GCGC 2 cut(s) 7, 151
BstKTI GATC 2 cut(s) 57, 190
BstMBI GATC 2 cut(s) 54, 187
BstMWI GCNNNNNNNGC 2 cut(s) 148, 248
BstNI CCWGG 1 cut(s) 196
BstSCI CCNGG 1 cut(s) 194
BstV1I GCAGC 1 cut(s) 154
BstV2I GAAGAC 1 cut(s) 286
BsuRI GGCC 4 cut(s) 194, 199, 251, 433
BtgZI GCGATG 2 cut(s) 257, 407
BtrI CACGTC 1 cut(s) 165
BtsCI GGATG 2 cut(s) 407, 411
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 2 cut(s) 41, 147
CfoI GCGC 2 cut(s) 7, 151
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 1 cut(s) 431
Csp6I GTAC 1 cut(s) 184
CviJI RGCY 9 cut(s) 25, 76, 90, 145, 194, 199, 251, 268, 433
CviKI_1 RGCY 9 cut(s) 25, 76, 90, 145, 194, 199, 251, 268, 433
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 1 cut(s) 91
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 56, 189
DpnII GATC 2 cut(s) 54, 187
EaeI YGGCCR 1 cut(s) 192
Eam1104I CTCTTC 2 cut(s) 254, 287
EarI CTCTTC 2 cut(s) 254, 287
Ecl136II GAGCTC 1 cut(s) 268
Eco130I CCWWGG 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 270
Eco53kI GAGCTC 1 cut(s) 268
Eco72I CACGTG 1 cut(s) 211
EcoICRI GAGCTC 1 cut(s) 268
EcoO109I RGGNCCY 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 270
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 252
FaiI YATR 1 cut(s) 219
FaqI GGGAC 1 cut(s) 427
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 1 cut(s) 143
FokI GGATG 2 cut(s) 414, 418
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 1 cut(s) 143
GlaI GCGC 2 cut(s) 6, 150
GluI GCNGC 1 cut(s) 143
GsuI CTGGAG 1 cut(s) 197
HaeII RGCGCY 2 cut(s) 8, 152
HaeIII GGCC 4 cut(s) 194, 199, 251, 433
HapII CCGG 2 cut(s) 146, 191
HhaI GCGC 2 cut(s) 7, 151
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 5, 149
HinP1I GCGC 2 cut(s) 5, 149
HincII GTYRAC 1 cut(s) 168
HindII GTYRAC 1 cut(s) 168
HinfI GANTC 1 cut(s) 344
HpaII CCGG 2 cut(s) 146, 191
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 1 cut(s) 168
Hpy8I GTNNAC 1 cut(s) 168
Hpy99I CGWCG 2 cut(s) 169, 172
HpyAV CCTTC 2 cut(s) 36, 232
HpyCH4III ACNGT 2 cut(s) 84, 172
HpyCH4IV ACGT 2 cut(s) 164, 210
HpyCH4V TGCA 3 cut(s) 49, 127, 142
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 248
HpyF3I CTNAG 1 cut(s) 91
HpySE526I ACGT 2 cut(s) 164, 210
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 5, 149
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 1 cut(s) 145
KroNI GCCGGC 1 cut(s) 147
Kzo9I GATC 2 cut(s) 54, 187
LpnPI CCDG 8 cut(s) 80, 137, 159, 161, 181, 204, 208, 348
Lsp1109I GCAGC 1 cut(s) 154
LweI GCATC 2 cut(s) 114, 122
MaeII ACGT 2 cut(s) 164, 210
MalI GATC 2 cut(s) 56, 189
MboI GATC 2 cut(s) 54, 187
MboII GAAGA 6 cut(s) 21, 244, 247, 271, 291, 304
MhlI GDGCHC 1 cut(s) 270
Mly113I GGCGCC 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 145
MspI CCGG 2 cut(s) 146, 191
MspR9I CCNGG 1 cut(s) 196
MvaI CCWGG 1 cut(s) 196
MwoI GCNNNNNNNGC 2 cut(s) 148, 248
NaeI GCCGGC 1 cut(s) 147
NarI GGCGCC 1 cut(s) 5
NdeII GATC 2 cut(s) 54, 187
NgoMIV GCCGGC 1 cut(s) 145
NlaIV GGNNCC 1 cut(s) 6
PdiI GCCGGC 1 cut(s) 147
PfeI GAWTC 1 cut(s) 344
PkrI GCNGC 1 cut(s) 144
PluTI GGCGCC 1 cut(s) 8
PmaCI CACGTG 1 cut(s) 211
PmlI CACGTG 1 cut(s) 211
Ppu21I YACGTR 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 194
PspCI CACGTG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 431
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
SacI GAGCTC 1 cut(s) 270
SalI GTCGAC 1 cut(s) 166
SatI GCNGC 1 cut(s) 143
Sau3AI GATC 2 cut(s) 54, 187
Sau96I GGNCC 1 cut(s) 431
ScrFI CCNGG 1 cut(s) 196
SduI GDGCHC 1 cut(s) 270
SetI ASST 5 cut(s) 92, 167, 213, 270, 353
SfaNI GCATC 2 cut(s) 114, 122
SfoI GGCGCC 1 cut(s) 6
SgrDI CGTCGACG 1 cut(s) 166
SsiI CCGC 1 cut(s) 137
SspDI GGCGCC 1 cut(s) 4
SstI GAGCTC 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 194
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 2 cut(s) 84, 172
TaiI ACGT 2 cut(s) 167, 213
TaqI TCGA 3 cut(s) 32, 167, 270
TfiI GAWTC 1 cut(s) 344
TscAI CASTG 1 cut(s) 99
TseI GCWGC 1 cut(s) 142
TspRI CASTG 1 cut(s) 99
XmiI GTMKAC 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.