RLG00000029127

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0030902
Physical Location & Seq
Reverse (-)
33434523 .. 33437225
2703 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029127

Sequence Viewer

Length: 921 bp
ATGAACAATCTAAGAGAACAGAACCCTGAGAACCATAAATCTAAGATAAAGAGTGGTAGAGAACTCGAAGAAGAGATTTCAGTGCTGGGGGATTCAAAGACATCGATTTCGAAGAATAGAACTCCAGAGGAGGAGCAAAGGCAGCTCTTAATAAGCCACCTGGTACTAATCTATGGCTTCTATGACCAATCCAGGAGCTTCTTCCTCCTCTTCTTCCACCCGTTGACCCATTCATATGCATATGATGTCTTCCTTAGCTATAATAGCGAGGGTACAAGTGCCTTTACTTGCCATTTGCACAGTGCTTTGTGTGGGATGGGAATTAACACCTACTTGCATGAGGATCCAGTTAGAGAGCAGGAAATATCAGCAATATTTCTCGAAGTATTTGAACTATCAAAGATTTCCCTGATTGTATTCTCTGAAACTTTGGCATCCTCAACATGGTTGGATCAACTTGGAGAGATCATTCAATGTAGAAAATCGAAGCAGCAATTGGTTCAGCCGATTTTCTACAAGGTGGATCCGACAAATCTACAAGATCATAGTGTGCTCTTTCAATATAGCAAATTTGTCACGTTGGTGTTTCTTGGACGGTTTATTCACATGCTGGTCACGGATATCTCATCCACATTATCTAATGGGACATCTTTGGATATGGCAATGTATAAAGTTGGAATGGATCCTCGTAGACGAGATATGTGTTATCTTTTAAACATTGGGGAAAATTCGGTCCATGTGGTAGGAATATGGGGGATTGGGGGAGTTGGCAAGCCAACAATTGCTAAATCAGTTTATAGTTCAAATGTTCAATTGCCTATAGGTTTGAAGGAACTTGTTTTTTGGCCAATGTTAGAGAAAAGTCAATGCTGGATGAAGGCCTTGTCCAACTACAAGAAACTCTACTTACTGATATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

307

Amino Acids

35.06

Weight (kDa)

6.55

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 80 - 180 4.6e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030902)

Species Orthologous Gene IDs
rosa_laevigata RLG00000029127
rosa_roxburghii Rroxscaffold_4G00312580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 691
AclWI GGATC 7 cut(s) 338, 351, 459, 518, 531, 677, 690
AcoI YGGCCR 1 cut(s) 845
AcsI RAATTY 2 cut(s) 569, 727
AfaI GTAC 2 cut(s) 165, 274
AfiI CCNNNNNNNGG 1 cut(s) 444
AgsI TTSAA 7 cut(s) 96, 392, 473, 560, 804, 812, 829
AjnI CCWGG 2 cut(s) 159, 191
AjuI GAANNNNNNNTTGG 2 cut(s) 479, 511
AleI CACNNNNGTG 1 cut(s) 581
AluBI AGCT 3 cut(s) 145, 198, 258
AluI AGCT 3 cut(s) 145, 198, 258
Alw21I GWGCWC 1 cut(s) 555
AlwI GGATC 7 cut(s) 338, 351, 459, 518, 531, 677, 690
AoxI GGCC 2 cut(s) 845, 879
ApeKI GCWGC 2 cut(s) 142, 490
ApoI RAATTY 2 cut(s) 569, 727
ArsI GACNNNNNNTTYG 2 cut(s) 91, 123
AspS9I GGNCC 1 cut(s) 733
AsuII TTCGAA 1 cut(s) 110
AvaII GGWCC 1 cut(s) 733
BalI TGGCCA 1 cut(s) 847
BamHI GGATCC 3 cut(s) 343, 523, 682
BbsI GAAGAC 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 2 cut(s) 154, 502
BccI CCATC 1 cut(s) 310
BciT130I CCWGG 2 cut(s) 161, 193
BfaI CTAG 1 cut(s) 919
BfmI CTRYAG 1 cut(s) 819
BisI GCNGC 2 cut(s) 143, 491
BlsI GCNGC 2 cut(s) 144, 492
Bme1390I CCNGG 2 cut(s) 161, 193
Bme18I GGWCC 1 cut(s) 733
BmgT120I GGNCC 1 cut(s) 733
BmiI GGNNCC 3 cut(s) 345, 525, 684
BmrFI CCNGG 2 cut(s) 161, 193
BmsI GCATC 1 cut(s) 443
BpiI GAAGAC 1 cut(s) 241
BpmI CTGGAG 1 cut(s) 108
Bpu10I CCTNAGC 1 cut(s) 254
Bpu14I TTCGAA 1 cut(s) 110
Bsa29I ATCGAT 1 cut(s) 104
Bsc4I CCNNNNNNNGG 1 cut(s) 444
Bse1I ACTGG 1 cut(s) 347
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 2 cut(s) 161, 193
BseCI ATCGAT 1 cut(s) 104
BseGI GGATG 4 cut(s) 321, 434, 626, 879
BseLI CCNNNNNNNGG 1 cut(s) 444
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 1 cut(s) 18
BseNI ACTGG 1 cut(s) 347
BseRI GAGGAG 3 cut(s) 143, 146, 197
BseXI GCAGC 2 cut(s) 154, 502
BseYI CCCAGC 1 cut(s) 85
BshFI GGCC 2 cut(s) 847, 881
BshVI ATCGAT 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 555
BslFI GGGAC 1 cut(s) 658
BslI CCNNNNNNNGG 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 658
BsnI GGCC 2 cut(s) 847, 881
Bsp119I TTCGAA 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 555
Bsp143I GATC 6 cut(s) 343, 451, 465, 523, 541, 682
BspANI GGCC 2 cut(s) 847, 881
BspCNI CTCAG 1 cut(s) 19
BspDI ATCGAT 1 cut(s) 104
BspLI GGNNCC 3 cut(s) 345, 525, 684
BspPI GGATC 7 cut(s) 338, 351, 459, 518, 531, 677, 690
BspT104I TTCGAA 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 669
BsrI ACTGG 1 cut(s) 347
BssMI GATC 6 cut(s) 343, 451, 465, 523, 541, 682
Bst2UI CCWGG 2 cut(s) 161, 193
Bst4CI ACNGT 2 cut(s) 302, 597
Bst6I CTCTTC 2 cut(s) 66, 215
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 1 cut(s) 773
BstDEI CTNAG 4 cut(s) 11, 27, 42, 254
BstF5I GGATG 4 cut(s) 321, 434, 626, 879
BstKTI GATC 6 cut(s) 346, 454, 468, 526, 544, 685
BstMBI GATC 6 cut(s) 343, 451, 465, 523, 541, 682
BstMWI GCNNNNNNNGC 2 cut(s) 142, 264
BstNI CCWGG 2 cut(s) 161, 193
BstNSI RCATGY 1 cut(s) 610
BstSCI CCNGG 2 cut(s) 159, 191
BstSFI CTRYAG 1 cut(s) 819
BstV1I GCAGC 2 cut(s) 154, 502
BstV2I GAAGAC 1 cut(s) 241
BstX2I RGATCY 3 cut(s) 343, 523, 682
BstYI RGATCY 3 cut(s) 343, 523, 682
Bsu15I ATCGAT 1 cut(s) 104
BsuRI GGCC 2 cut(s) 847, 881
BsuTUI ATCGAT 1 cut(s) 104
BtsCI GGATG 4 cut(s) 321, 434, 626, 879
BtsIMutI CAGTG 2 cut(s) 87, 307
Cac8I GCNNGC 1 cut(s) 773
Cfr13I GGNCC 1 cut(s) 733
ClaI ATCGAT 1 cut(s) 104
CsiI ACCWGGT 1 cut(s) 159
Csp6I GTAC 2 cut(s) 164, 273
CviAII CATG 4 cut(s) 338, 444, 607, 737
CviJI RGCY 9 cut(s) 145, 156, 177, 198, 258, 505, 775, 847, 881
CviKI_1 RGCY 9 cut(s) 145, 156, 177, 198, 258, 505, 775, 847, 881
CviQI GTAC 2 cut(s) 164, 273
DdeI CTNAG 4 cut(s) 11, 27, 42, 254
DpnI GATC 6 cut(s) 345, 453, 467, 525, 543, 684
DpnII GATC 6 cut(s) 343, 451, 465, 523, 541, 682
DraI TTTAAA 1 cut(s) 714
EaeI YGGCCR 1 cut(s) 845
Eam1104I CTCTTC 2 cut(s) 66, 215
EarI CTCTTC 2 cut(s) 66, 215
Eco147I AGGCCT 1 cut(s) 881
Eco32I GATATC 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 733
EcoRII CCWGG 2 cut(s) 159, 191
EcoRV GATATC 1 cut(s) 622
EcoT22I ATGCAT 1 cut(s) 241
FaeI CATG 4 cut(s) 341, 447, 610, 740
FaqI GGGAC 1 cut(s) 658
FatI CATG 4 cut(s) 337, 443, 606, 736
FauNDI CATATG 2 cut(s) 235, 241
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 2 cut(s) 143, 491
FokI GGATG 4 cut(s) 328, 421, 613, 886
Fsp4HI GCNGC 2 cut(s) 143, 491
FspBI CTAG 1 cut(s) 919
GluI GCNGC 2 cut(s) 143, 491
GsaI CCCAGC 1 cut(s) 89
GsuI CTGGAG 1 cut(s) 108
HaeIII GGCC 2 cut(s) 847, 881
Hin1II CATG 4 cut(s) 341, 447, 610, 740
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HinfI GANTC 1 cut(s) 92
Hpy166II GTNNAC 2 cut(s) 225, 692
Hpy188I TCNGA 2 cut(s) 424, 528
Hpy188III TCNNGA 2 cut(s) 125, 380
Hpy8I GTNNAC 2 cut(s) 225, 692
HpyAV CCTTC 2 cut(s) 823, 871
HpyCH4III ACNGT 2 cut(s) 302, 597
HpyCH4IV ACGT 1 cut(s) 578
HpyCH4V TGCA 3 cut(s) 239, 298, 337
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 264
HpyF3I CTNAG 4 cut(s) 11, 27, 42, 254
HpySE526I ACGT 1 cut(s) 578
Hsp92II CATG 4 cut(s) 341, 447, 610, 740
Kzo9I GATC 6 cut(s) 343, 451, 465, 523, 541, 682
LmnI GCTCC 2 cut(s) 133, 195
Lsp1109I GCAGC 2 cut(s) 154, 502
LweI GCATC 1 cut(s) 443
MabI ACCWGGT 1 cut(s) 159
MaeI CTAG 1 cut(s) 919
MaeII ACGT 1 cut(s) 578
MaeIII GTNAC 2 cut(s) 574, 613
MalI GATC 6 cut(s) 345, 453, 467, 525, 543, 684
MboI GATC 6 cut(s) 343, 451, 465, 523, 541, 682
MboII GAAGA 7 cut(s) 80, 83, 124, 193, 202, 205, 241
MfeI CAATTG 3 cut(s) 494, 780, 812
MflI RGATCY 3 cut(s) 343, 523, 682
MhlI GDGCHC 1 cut(s) 555
MlsI TGGCCA 1 cut(s) 847
MluCI AATT 6 cut(s) 321, 494, 569, 727, 780, 812
MluNI TGGCCA 1 cut(s) 847
MmeI TCCRAC 4 cut(s) 429, 551, 655, 912
MnlI CCTC 8 cut(s) 121, 124, 215, 218, 262, 334, 448, 696
Mox20I TGGCCA 1 cut(s) 847
Mph1103I ATGCAT 1 cut(s) 241
MscI TGGCCA 1 cut(s) 847
MseI TTAA 3 cut(s) 149, 324, 713
MslI CAYNNNNRTG 2 cut(s) 234, 581
Msp20I TGGCCA 1 cut(s) 847
MspR9I CCNGG 2 cut(s) 161, 193
MunI CAATTG 3 cut(s) 494, 780, 812
MvaI CCWGG 2 cut(s) 161, 193
MwoI GCNNNNNNNGC 2 cut(s) 142, 264
NdeI CATATG 2 cut(s) 235, 241
NdeII GATC 6 cut(s) 343, 451, 465, 523, 541, 682
NlaIII CATG 4 cut(s) 341, 447, 610, 740
NlaIV GGNNCC 3 cut(s) 345, 525, 684
NmuCI GTSAC 2 cut(s) 574, 613
NsiI ATGCAT 1 cut(s) 241
NspI RCATGY 1 cut(s) 610
NspV TTCGAA 1 cut(s) 110
OliI CACNNNNGTG 1 cut(s) 581
PceI AGGCCT 1 cut(s) 881
PfeI GAWTC 1 cut(s) 92
PfoI TCCNGGA 1 cut(s) 191
PkrI GCNGC 2 cut(s) 144, 492
Psp6I CCWGG 2 cut(s) 159, 191
PspFI CCCAGC 1 cut(s) 85
PspGI CCWGG 2 cut(s) 159, 191
PspN4I GGNNCC 3 cut(s) 345, 525, 684
PspPI GGNCC 1 cut(s) 733
PsuI RGATCY 3 cut(s) 343, 523, 682
RsaI GTAC 2 cut(s) 165, 274
RsaNI GTAC 2 cut(s) 164, 273
RseI CAYNNNNRTG 2 cut(s) 234, 581
SaqAI TTAA 3 cut(s) 149, 324, 713
SatI GCNGC 2 cut(s) 143, 491
Sau3AI GATC 6 cut(s) 343, 451, 465, 523, 541, 682
Sau96I GGNCC 1 cut(s) 733
ScrFI CCNGG 2 cut(s) 161, 193
SduI GDGCHC 1 cut(s) 555
SetI ASST 8 cut(s) 147, 162, 200, 260, 332, 522, 581, 826
SexAI ACCWGGT 1 cut(s) 159
SfaNI GCATC 1 cut(s) 443
SfcI CTRYAG 1 cut(s) 819
SfuI TTCGAA 1 cut(s) 110
SinI GGWCC 1 cut(s) 733
SmiMI CAYNNNNRTG 2 cut(s) 234, 581
Sse9I AATT 6 cut(s) 321, 494, 569, 727, 780, 812
SseBI AGGCCT 1 cut(s) 881
SspI AATATT 1 cut(s) 375
SspMI CTAG 1 cut(s) 919
StuI AGGCCT 1 cut(s) 881
StyD4I CCNGG 2 cut(s) 159, 191
TaaI ACNGT 2 cut(s) 302, 597
TaiI ACGT 1 cut(s) 581
TaqI TCGA 5 cut(s) 66, 104, 110, 381, 485
TaqII GACCGA 1 cut(s) 721
TasI AATT 6 cut(s) 321, 494, 569, 727, 780, 812
TfiI GAWTC 1 cut(s) 92
Tru1I TTAA 3 cut(s) 149, 324, 713
Tru9I TTAA 3 cut(s) 149, 324, 713
TscAI CASTG 2 cut(s) 87, 307
TseFI GTSAC 2 cut(s) 574, 613
TseI GCWGC 2 cut(s) 142, 490
Tsp45I GTSAC 2 cut(s) 574, 613
TspDTI ATGAA 3 cut(s) 17, 222, 890
TspGWI ACGGA 1 cut(s) 632
TspRI CASTG 2 cut(s) 87, 307
VpaK11BI GGWCC 1 cut(s) 733
XapI RAATTY 2 cut(s) 569, 727
XceI RCATGY 1 cut(s) 610
XmiI GTMKAC 1 cut(s) 691
XspI CTAG 1 cut(s) 919
Zsp2I ATGCAT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.