Rroxscaffold_4G00312580

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0030902
Physical Location & Seq
Reverse (-)
35604152 .. 35608130
3979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312580.1

Sequence Viewer

Length: 477 bp
ATGGAGTTCCACTGGACTCTTCCATACTCTGAAATCGGTCTAGACTCCGAGGAGGAGCAAAGGCAGCTCTTAATAAGCCACCTGGTACTAATCTATGGCTTCTATGACCAATCCAGGAGCTTCTTCCTCCTCTTCTTCAACCCATTGACCCATTCATATGCATATGATGTCTTCTCAGCTATAATAGCGAGGATAGAAGTGCCTTTACGTGCCATTTGCACAGTGCTTTGTGTGGGATGGGAATTAGCGTACACCTACTTGCATGAGGAGCCAGTTAGAGAGGAGGACATATCAGCAATATTTCTCCAAGTATTTGAACTATCAAAGATTTCCCCGATTGTATTCTCCGAAACTTTGGCATCCTCAACATGGTTGGATCAACTTGGAGAGATCATTCAATGTAGAAAATCGAAGCAGCGCGCAATTGGTTCAGCCGATTTTCTACAAGGTGGATCCGACAAATCTACAAGATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

158

Amino Acids

18.17

Weight (kDa)

4.82

Isoelectric Point (pI)

59.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030902)

Species Orthologous Gene IDs
rosa_laevigata RLG00000029127
rosa_roxburghii Rroxscaffold_4G00312580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 420
AclWI GGATC 3 cut(s) 384, 447, 460
AfaI GTAC 2 cut(s) 87, 251
AfiI CCNNNNNNNGG 1 cut(s) 369
AgsI TTSAA 3 cut(s) 139, 317, 398
AjnI CCWGG 2 cut(s) 81, 113
AluBI AGCT 3 cut(s) 67, 120, 179
AluI AGCT 3 cut(s) 67, 120, 179
AlwI GGATC 3 cut(s) 384, 447, 460
ApeKI GCWGC 2 cut(s) 64, 415
AspLEI GCGC 2 cut(s) 420, 422
BamHI GGATCC 1 cut(s) 452
BarI GAAGNNNNNNTAC 2 cut(s) 189, 221
BbsI GAAGAC 1 cut(s) 163
BbvI GCAGC 2 cut(s) 76, 427
BccI CCATC 1 cut(s) 231
BciT130I CCWGG 2 cut(s) 83, 115
BfaI CTAG 1 cut(s) 41
BisI GCNGC 2 cut(s) 65, 416
BlsI GCNGC 2 cut(s) 66, 417
Bme1390I CCNGG 2 cut(s) 83, 115
BmiI GGNNCC 2 cut(s) 270, 454
BmrFI CCNGG 2 cut(s) 83, 115
BmsI GCATC 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 163
BsaAI YACGTR 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 48
Bsc4I CCNNNNNNNGG 1 cut(s) 369
Bse1I ACTGG 2 cut(s) 17, 272
BseBI CCWGG 2 cut(s) 83, 115
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 2 cut(s) 242, 359
BseLI CCNNNNNNNGG 1 cut(s) 369
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 2 cut(s) 17, 272
BsePI GCGCGC 1 cut(s) 418
BseRI GAGGAG 5 cut(s) 65, 68, 119, 281, 296
BseXI GCAGC 2 cut(s) 76, 427
Bsh1236I CGCG 1 cut(s) 420
BslI CCNNNNNNNGG 1 cut(s) 369
Bsp143I GATC 4 cut(s) 376, 390, 452, 470
BspCNI CTCAG 1 cut(s) 188
BspFNI CGCG 1 cut(s) 420
BspLI GGNNCC 2 cut(s) 270, 454
BspPI GGATC 3 cut(s) 384, 447, 460
BsrI ACTGG 2 cut(s) 17, 272
BssECI CCNNGG 1 cut(s) 48
BssHII GCGCGC 1 cut(s) 418
BssMI GATC 4 cut(s) 376, 390, 452, 470
Bst2UI CCWGG 2 cut(s) 83, 115
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 2 cut(s) 24, 137
BstBAI YACGTR 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 1 cut(s) 175
BstF5I GGATG 2 cut(s) 242, 359
BstFNI CGCG 1 cut(s) 420
BstHHI GCGC 2 cut(s) 420, 422
BstKTI GATC 4 cut(s) 379, 393, 455, 473
BstMBI GATC 4 cut(s) 376, 390, 452, 470
BstMWI GCNNNNNNNGC 3 cut(s) 64, 185, 268
BstNI CCWGG 2 cut(s) 83, 115
BstSCI CCNGG 2 cut(s) 81, 113
BstUI CGCG 1 cut(s) 420
BstV1I GCAGC 2 cut(s) 76, 427
BstV2I GAAGAC 1 cut(s) 163
BstX2I RGATCY 1 cut(s) 452
BstYI RGATCY 1 cut(s) 452
BtsCI GGATG 2 cut(s) 242, 359
BtsIMutI CAGTG 2 cut(s) 10, 228
Cac8I GCNNGC 1 cut(s) 420
CfoI GCGC 2 cut(s) 420, 422
CsiI ACCWGGT 1 cut(s) 81
Csp6I GTAC 2 cut(s) 86, 250
CviAII CATG 2 cut(s) 263, 369
CviJI RGCY 7 cut(s) 67, 78, 99, 120, 179, 271, 434
CviKI_1 RGCY 7 cut(s) 67, 78, 99, 120, 179, 271, 434
CviQI GTAC 2 cut(s) 86, 250
DdeI CTNAG 1 cut(s) 175
DpnI GATC 4 cut(s) 378, 392, 454, 472
DpnII GATC 4 cut(s) 376, 390, 452, 470
Eam1104I CTCTTC 2 cut(s) 24, 137
EarI CTCTTC 2 cut(s) 24, 137
EcoRII CCWGG 2 cut(s) 81, 113
EcoT22I ATGCAT 1 cut(s) 163
FaeI CATG 2 cut(s) 266, 372
FatI CATG 2 cut(s) 262, 368
FauNDI CATATG 2 cut(s) 157, 163
Fnu4HI GCNGC 2 cut(s) 65, 416
FokI GGATG 2 cut(s) 249, 346
Fsp4HI GCNGC 2 cut(s) 65, 416
FspBI CTAG 1 cut(s) 41
GlaI GCGC 2 cut(s) 419, 421
GluI GCNGC 2 cut(s) 65, 416
HhaI GCGC 2 cut(s) 420, 422
Hin1II CATG 2 cut(s) 266, 372
Hin6I GCGC 2 cut(s) 418, 420
HinP1I GCGC 2 cut(s) 418, 420
HinfI GANTC 2 cut(s) 16, 44
Hpy166II GTNNAC 1 cut(s) 252
Hpy188I TCNGA 4 cut(s) 31, 49, 349, 457
Hpy188III TCNNGA 1 cut(s) 41
Hpy8I GTNNAC 1 cut(s) 252
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 208
HpyCH4V TGCA 3 cut(s) 161, 219, 262
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 185, 268
HpyF3I CTNAG 1 cut(s) 175
HpySE526I ACGT 1 cut(s) 208
Hsp92II CATG 2 cut(s) 266, 372
HspAI GCGC 2 cut(s) 418, 420
Kzo9I GATC 4 cut(s) 376, 390, 452, 470
LmnI GCTCC 3 cut(s) 55, 117, 268
LpnPI CCDG 5 cut(s) 68, 95, 100, 127, 285
Lsp1109I GCAGC 2 cut(s) 76, 427
LweI GCATC 1 cut(s) 368
MabI ACCWGGT 1 cut(s) 81
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 208
MalI GATC 4 cut(s) 378, 392, 454, 472
MboI GATC 4 cut(s) 376, 390, 452, 470
MboII GAAGA 5 cut(s) 11, 115, 124, 127, 163
MfeI CAATTG 1 cut(s) 423
MflI RGATCY 1 cut(s) 452
MluCI AATT 2 cut(s) 242, 423
MlyI GAGTC 2 cut(s) 10, 38
MmeI TCCRAC 1 cut(s) 354
MnlI CCTC 9 cut(s) 43, 46, 137, 140, 183, 259, 274, 277, 373
Mph1103I ATGCAT 1 cut(s) 163
MseI TTAA 1 cut(s) 71
MslI CAYNNNNRTG 1 cut(s) 156
MspR9I CCNGG 2 cut(s) 83, 115
MunI CAATTG 1 cut(s) 423
MvaI CCWGG 2 cut(s) 83, 115
MvnI CGCG 1 cut(s) 420
MwoI GCNNNNNNNGC 3 cut(s) 64, 185, 268
NdeI CATATG 2 cut(s) 157, 163
NdeII GATC 4 cut(s) 376, 390, 452, 470
NlaIII CATG 2 cut(s) 266, 372
NlaIV GGNNCC 2 cut(s) 270, 454
NsiI ATGCAT 1 cut(s) 163
PauI GCGCGC 1 cut(s) 418
PfoI TCCNGGA 1 cut(s) 113
PkrI GCNGC 2 cut(s) 66, 417
PleI GAGTC 2 cut(s) 10, 38
PpsI GAGTC 2 cut(s) 10, 38
Ppu21I YACGTR 1 cut(s) 209
Psp6I CCWGG 2 cut(s) 81, 113
PspGI CCWGG 2 cut(s) 81, 113
PspN4I GGNNCC 2 cut(s) 270, 454
PsuI RGATCY 1 cut(s) 452
PteI GCGCGC 1 cut(s) 418
RsaI GTAC 2 cut(s) 87, 251
RsaNI GTAC 2 cut(s) 86, 250
RseI CAYNNNNRTG 1 cut(s) 156
SaqAI TTAA 1 cut(s) 71
SatI GCNGC 2 cut(s) 65, 416
Sau3AI GATC 4 cut(s) 376, 390, 452, 470
SchI GAGTC 2 cut(s) 10, 38
ScrFI CCNGG 2 cut(s) 83, 115
SetI ASST 7 cut(s) 69, 84, 122, 181, 211, 257, 451
SexAI ACCWGGT 1 cut(s) 81
SfaNI GCATC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 156
Sse9I AATT 2 cut(s) 242, 423
SspI AATATT 1 cut(s) 300
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 81, 113
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 211
TaqI TCGA 1 cut(s) 410
TaqII GACCGA 1 cut(s) 26
TasI AATT 2 cut(s) 242, 423
Tru1I TTAA 1 cut(s) 71
Tru9I TTAA 1 cut(s) 71
TscAI CASTG 2 cut(s) 17, 228
TseI GCWGC 2 cut(s) 64, 415
TspDTI ATGAA 1 cut(s) 144
TspRI CASTG 2 cut(s) 17, 228
XbaI TCTAGA 1 cut(s) 40
XspI CTAG 1 cut(s) 41
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.