RLG00000030593

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0022987
Physical Location & Seq
Reverse (-)
64020024 .. 64021371
1348 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030593

Sequence Viewer

Length: 1212 bp
ATGGAATCAAGAGATGGCGAAACTAGTAAGAGGGCAAATGTGATCAAGGAGCTGAAGTATAGGATCTCAGAAGTGGAAAGATGGTCAGAGAACTTGAGCATATTTAGAGTTCGAAATAAAGTTCGAAAAGTGAAGGAGGATGCCTATAACCCTCGCCTTGTCTCAATCGGACTTTATCACGAAGACAAACATGAACTTGAAGCTATGAGAGAGCTTAAAGAGCGTTATATGCAATGTTTCTTTCAGCGAGCAAAGGATCCTCAGGAATGCTGGGAAGAGTGCGCTCTTGCTATTAATGGTTTACAGACAGATATTCGTGCCTGCTACAATGGAAGCATCAAGGAAATGTCAAACAATGCGGTTGATGAAAAATTGCTCCTTGATGGTTGTTTCTTATTGGAGCTTTTCCTTAGGTATGACCAACACAGCAACAATAACAATATGAGTCCAGGTGATCATGATCCAATTTTTAGTAGTGCTTGGATCATCCCAACTCTTCGGCGTGATCTTGCATTACTCGAAAACCAGATCCCGTTTTTTGTTCTTATTAAGCTGCATGAAATATATTTAACACTGAGGGACATGCATCATGACTCTATAACACCAAAATCGACCATCGGTTACCATGCACTTGTTTTCTTTCTAACTATGCACACAAAAGCAATACATGCAGCATATGCAGCGGGGCTGAGTGAAAATGAGTGCAAGCATTTGCTTGATCTCTTGCACAAAATCTATCTCCCAAATTTCTTCTCTCCCAGCCCAACCCCCATCTCTGTAGAGTCGACTACCAAAGATGGTTATAGTCTAAGCAACAGAAACTGGGGATTCAATTTTTCCACAGCAGAACTTTTCGAGGCTGGAATCGAGTTTGAACTACAGCAGGAGGACATGCTTCACATGAGTTTCGAGAAGGAGGTAATTAGAATCCCTCCGTTGGTGATGGATGAGTCAACGAATTCAATGATGAGGAATTTAATTGCTTATGAGCAGTGTGGTTTCGTAAAAAATTACTTTACTTCATTTGCCATCGTCCTGAAAAGTCTGATACGATCGTTGGAGGATATTAAACTACTTCGGCAAAGAAAGATCATAAGTTCCACTTGGACTGCTGATGAAGAATGTTGGATTCATTTCAACACTATGCTGGATGAAGTTGTTGTGAAGGAGTTGCAAAACATGTACAACTCACCCGGCCAAATTGATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

404

Amino Acids

47.03

Weight (kDa)

5.67

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 34 - 395 1.1e-81 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022987)

Species Orthologous Gene IDs
rosa_laevigata RLG00000030593
rosa_multiflora Rmu_sc0003008.1_g000015
rosa_samantha Rh1DG018900
rosa_wichuraiana Rw1G001540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 785
AciI CCGC 2 cut(s) 359, 683
AclWI GGATC 6 cut(s) 71, 251, 264, 455, 491, 523
AcoI YGGCCR 1 cut(s) 1195
AcsI RAATTY 3 cut(s) 745, 958, 973
AcuI CTGAAG 1 cut(s) 74
AfaI GTAC 1 cut(s) 1184
AfiI CCNNNNNNNGG 1 cut(s) 937
AflIII ACRYGT 1 cut(s) 1179
AgsI TTSAA 5 cut(s) 200, 832, 875, 963, 1138
AhlI ACTAGT 1 cut(s) 23
AjnI CCWGG 1 cut(s) 448
AluBI AGCT 5 cut(s) 52, 203, 214, 403, 553
AluI AGCT 5 cut(s) 52, 203, 214, 403, 553
Alw26I GTCTC 1 cut(s) 166
AlwI GGATC 6 cut(s) 71, 251, 264, 455, 491, 523
AlwNI CAGNNNCTG 1 cut(s) 822
AoxI GGCC 1 cut(s) 1195
ApeKI GCWGC 3 cut(s) 553, 671, 680
ApoI RAATTY 3 cut(s) 745, 958, 973
AseI ATTAAT 1 cut(s) 294
AspLEI GCGC 1 cut(s) 284
AsuC2I CCSGG 1 cut(s) 1194
AsuHPI GGTGA 3 cut(s) 464, 952, 1182
AsuII TTCGAA 2 cut(s) 112, 124
AxyI CCTNAGG 2 cut(s) 261, 410
BaeI ACNNNNGTAYC 2 cut(s) 1040, 1073
BamHI GGATCC 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 189
BbvI GCAGC 3 cut(s) 540, 683, 692
BccI CCATC 8 cut(s) 8, 75, 377, 623, 779, 791, 937, 1037
BciT130I CCWGG 1 cut(s) 450
BclI TGATCA 2 cut(s) 42, 454
BcnI CCSGG 1 cut(s) 1194
BcoDI GTCTC 1 cut(s) 166
BcuI ACTAGT 1 cut(s) 23
BfaI CTAG 1 cut(s) 24
BfmI CTRYAG 2 cut(s) 777, 878
BisI GCNGC 3 cut(s) 554, 672, 681
BlsI GCNGC 3 cut(s) 555, 673, 682
Bme1390I CCNGG 2 cut(s) 450, 1194
BmiI GGNNCC 1 cut(s) 258
BmrFI CCNGG 2 cut(s) 450, 1194
BmrI ACTGGG 1 cut(s) 832
BmsI GCATC 3 cut(s) 130, 345, 595
BmuI ACTGGG 1 cut(s) 832
BpiI GAAGAC 1 cut(s) 189
Bpu14I TTCGAA 2 cut(s) 112, 124
BpuEI CTTGAG 1 cut(s) 115
BpuMI CCSGG 1 cut(s) 1194
BsaBI GATNNNNATC 1 cut(s) 459
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 1 cut(s) 937
Bse1I ACTGG 1 cut(s) 827
Bse21I CCTNAGG 2 cut(s) 261, 410
Bse3DI GCAATG 1 cut(s) 239
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 1 cut(s) 450
BseGI GGATG 4 cut(s) 145, 486, 952, 1156
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 1 cut(s) 937
BseMI GCAATG 1 cut(s) 239
BseMII CTCAG 4 cut(s) 81, 275, 566, 680
BseNI ACTGG 1 cut(s) 827
BseXI GCAGC 3 cut(s) 540, 683, 692
BseYI CCCAGC 2 cut(s) 270, 758
Bsh1285I CGRYCG 1 cut(s) 1055
BshFI GGCC 1 cut(s) 1197
BsiEI CGRYCG 1 cut(s) 1055
BsiSI CCGG 1 cut(s) 1194
BslFI GGGAC 1 cut(s) 593
BslI CCNNNNNNNGG 1 cut(s) 937
BsmAI GTCTC 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 593
BsmI GAATGC 1 cut(s) 272
BsnI GGCC 1 cut(s) 1197
Bsp119I TTCGAA 2 cut(s) 112, 124
Bsp1407I TGTACA 1 cut(s) 1182
BspACI CCGC 2 cut(s) 359, 683
BspANI GGCC 1 cut(s) 1197
BspCNI CTCAG 4 cut(s) 80, 274, 567, 681
BspHI TCATGA 2 cut(s) 457, 589
BspLI GGNNCC 1 cut(s) 258
BspPI GGATC 6 cut(s) 71, 251, 264, 455, 491, 523
BspT104I TTCGAA 2 cut(s) 112, 124
BsrDI GCAATG 1 cut(s) 239
BsrGI TGTACA 1 cut(s) 1182
BsrI ACTGG 1 cut(s) 827
Bst2UI CCWGG 1 cut(s) 450
Bst6I CTCTTC 2 cut(s) 270, 501
BstAPI GCANNNNNTGC 2 cut(s) 668, 677
BstAUI TGTACA 1 cut(s) 1182
BstBI TTCGAA 2 cut(s) 112, 124
BstC8I GCNNGC 3 cut(s) 249, 322, 707
BstDEI CTNAG 6 cut(s) 67, 261, 410, 575, 689, 809
BstEII GGTNACC 1 cut(s) 620
BstF5I GGATG 4 cut(s) 145, 486, 952, 1156
BstHHI GCGC 1 cut(s) 284
BstMAI GTCTC 1 cut(s) 166
BstMCI CGRYCG 1 cut(s) 1055
BstMWI GCNNNNNNNGC 5 cut(s) 220, 229, 668, 677, 680
BstNI CCWGG 1 cut(s) 450
BstNSI RCATGY 4 cut(s) 586, 671, 895, 1183
BstPI GGTNACC 1 cut(s) 620
BstSCI CCNGG 2 cut(s) 448, 1192
BstSFI CTRYAG 2 cut(s) 777, 878
BstV1I GCAGC 3 cut(s) 540, 683, 692
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 3 cut(s) 63, 256, 528
BstYI RGATCY 3 cut(s) 63, 256, 528
Bsu36I CCTNAGG 2 cut(s) 261, 410
BsuRI GGCC 1 cut(s) 1197
BtsCI GGATG 4 cut(s) 145, 486, 952, 1156
BtsI GCAGTG 1 cut(s) 998
BtsIMutI CAGTG 2 cut(s) 572, 998
Cac8I GCNNGC 3 cut(s) 249, 322, 707
CaiI CAGNNNCTG 1 cut(s) 822
CciI TCATGA 2 cut(s) 457, 589
CfoI GCGC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 1183
CviJI RGCY 9 cut(s) 52, 203, 214, 403, 553, 688, 762, 860, 1197
CviKI_1 RGCY 9 cut(s) 52, 203, 214, 403, 553, 688, 762, 860, 1197
CviQI GTAC 1 cut(s) 1183
DdeI CTNAG 6 cut(s) 67, 261, 410, 575, 689, 809
EaeI YGGCCR 1 cut(s) 1195
Eam1104I CTCTTC 2 cut(s) 270, 501
EarI CTCTTC 2 cut(s) 270, 501
Eco57I CTGAAG 1 cut(s) 74
Eco81I CCTNAGG 2 cut(s) 261, 410
Eco91I GGTNACC 1 cut(s) 620
EcoO65I GGTNACC 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 958
EcoRII CCWGG 1 cut(s) 448
EcoT22I ATGCAT 1 cut(s) 588
FalI AAGNNNNNCTT 2 cut(s) 1087, 1119
FaqI GGGAC 1 cut(s) 593
FauI CCCGC 1 cut(s) 676
FauNDI CATATG 1 cut(s) 676
FbaI TGATCA 2 cut(s) 42, 454
FblI GTMKAC 1 cut(s) 785
Fnu4HI GCNGC 3 cut(s) 554, 672, 681
FokI GGATG 4 cut(s) 152, 473, 959, 1163
Fsp4HI GCNGC 3 cut(s) 554, 672, 681
FspBI CTAG 1 cut(s) 24
GlaI GCGC 1 cut(s) 283
GluI GCNGC 3 cut(s) 554, 672, 681
GsaI CCCAGC 2 cut(s) 274, 762
HaeIII GGCC 1 cut(s) 1197
HapII CCGG 1 cut(s) 1194
HhaI GCGC 1 cut(s) 284
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 2 cut(s) 786, 954
HindII GTYRAC 2 cut(s) 786, 954
HinfI GANTC 9 cut(s) 5, 445, 593, 782, 828, 864, 927, 950, 1129
HpaII CCGG 1 cut(s) 1194
HphI GGTGA 3 cut(s) 464, 952, 1182
Hpy166II GTNNAC 3 cut(s) 302, 786, 954
Hpy188I TCNGA 4 cut(s) 70, 88, 170, 1047
Hpy188III TCNNGA 7 cut(s) 9, 179, 263, 458, 590, 910, 1036
Hpy8I GTNNAC 3 cut(s) 302, 786, 954
HpyAV CCTTC 3 cut(s) 127, 907, 1159
HpyF10VI GCNNNNNNNGC 5 cut(s) 220, 229, 668, 677, 680
HpyF3I CTNAG 6 cut(s) 67, 261, 410, 575, 689, 809
HspAI GCGC 1 cut(s) 282
Ksp22I TGATCA 2 cut(s) 42, 454
LmnI GCTCC 3 cut(s) 49, 381, 400
Lsp1109I GCAGC 3 cut(s) 540, 683, 692
LweI GCATC 3 cut(s) 130, 345, 595
MaeI CTAG 1 cut(s) 24
MaeIII GTNAC 1 cut(s) 620
MboII GAAGA 5 cut(s) 194, 287, 488, 742, 1130
MflI RGATCY 3 cut(s) 63, 256, 528
MlyI GAGTC 4 cut(s) 454, 587, 791, 959
MmeI TCCRAC 2 cut(s) 1038, 1106
Mph1103I ATGCAT 1 cut(s) 588
MseI TTAA 6 cut(s) 216, 294, 549, 569, 977, 1068
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 1 cut(s) 1194
MspR9I CCNGG 2 cut(s) 450, 1194
Mva1269I GAATGC 1 cut(s) 272
MvaI CCWGG 1 cut(s) 450
MwoI GCNNNNNNNGC 5 cut(s) 220, 229, 668, 677, 680
NciI CCSGG 1 cut(s) 1194
NdeI CATATG 1 cut(s) 676
NlaIV GGNNCC 1 cut(s) 258
NsiI ATGCAT 1 cut(s) 588
NspI RCATGY 4 cut(s) 586, 671, 895, 1183
NspV TTCGAA 2 cut(s) 112, 124
PagI TCATGA 2 cut(s) 457, 589
PciI ACATGT 1 cut(s) 1179
PctI GAATGC 1 cut(s) 272
PfeI GAWTC 5 cut(s) 5, 828, 864, 927, 1129
PkrI GCNGC 3 cut(s) 555, 673, 682
Ple19I CGATCG 1 cut(s) 1055
PleI GAGTC 4 cut(s) 453, 587, 790, 958
PpsI GAGTC 4 cut(s) 453, 587, 790, 958
PscI ACATGT 1 cut(s) 1179
PshBI ATTAAT 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 448
PspEI GGTNACC 1 cut(s) 620
PspFI CCCAGC 2 cut(s) 270, 758
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 1 cut(s) 258
PstNI CAGNNNCTG 1 cut(s) 822
PsuI RGATCY 3 cut(s) 63, 256, 528
PvuI CGATCG 1 cut(s) 1055
RsaI GTAC 1 cut(s) 1184
RsaNI GTAC 1 cut(s) 1183
SalI GTCGAC 1 cut(s) 784
SaqAI TTAA 6 cut(s) 216, 294, 549, 569, 977, 1068
SatI GCNGC 3 cut(s) 554, 672, 681
SchI GAGTC 4 cut(s) 454, 587, 791, 959
ScrFI CCNGG 2 cut(s) 450, 1194
SetI ASST 8 cut(s) 54, 205, 216, 405, 416, 454, 555, 921
SfaNI GCATC 3 cut(s) 130, 345, 595
SfcI CTRYAG 2 cut(s) 777, 878
SfuI TTCGAA 2 cut(s) 112, 124
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
SpeI ACTAGT 1 cut(s) 23
SsiI CCGC 2 cut(s) 359, 683
SspMI CTAG 1 cut(s) 24
StyD4I CCNGG 2 cut(s) 448, 1192
TaqI TCGA 8 cut(s) 112, 124, 519, 611, 785, 855, 867, 909
TatI WGTACW 1 cut(s) 1182
TfiI GAWTC 5 cut(s) 5, 828, 864, 927, 1129
Tru1I TTAA 6 cut(s) 216, 294, 549, 569, 977, 1068
Tru9I TTAA 6 cut(s) 216, 294, 549, 569, 977, 1068
TscAI CASTG 2 cut(s) 579, 998
TseI GCWGC 3 cut(s) 553, 671, 680
TspDTI ATGAA 7 cut(s) 207, 381, 573, 1011, 1121, 1131, 1167
TspGWI ACGGA 1 cut(s) 924
TspRI CASTG 2 cut(s) 579, 998
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 3 cut(s) 745, 958, 973
XceI RCATGY 4 cut(s) 586, 671, 895, 1183
XmiI GTMKAC 1 cut(s) 785
XspI CTAG 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.