Rw1G001540

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0022987
Physical Location & Seq
Forward (+)
2708827 .. 2712421
3595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G001540.1

Sequence Viewer

Length: 1365 bp
ATGATGGAATCAAAAGATGGCGAAACTAGTAAGAGGGCAAATGTGATCAAGGAGCTGAAGTATAGGATCTCAGAGGTGGAAAGATGGTCAGAGAACTTGAGCATATATAGAGTTCGAAATAATGTTCGAAAAGTGAAGGAGGATGCCTATAACCCTCGCCTCGTCTCAATCGGACTTTATCACGAAGACAAACATGAACTTGAAGGTATTAGAGAGCTTAAAGAGCATTATATGCAATGTTTCTTTCGGCGAGCAAAGGATAGTCAGGAATGCTTGGAAGAGTGCGCTCTTGCTATTAATGGTTTACAGAAAGATATTCGTGCCTGCTACGATGGAAGCATCAAGGACATGTCAAACAATGCGGTTGATGAAAAATTGCTCCTTGATGGTTGTTTCTTATTGGAGCTTTTCCTTAGGTATGACCAACACAGCAACAATAACAATATGAGTCCAGGTGATCATGATCCAATTTTTAGTAGTGCTTGGATCATCCCAGCTCTTCGGCGTGATCTTGCATTACTCGAAAACCAGATCCCCTTTTTTGTTCTTATTAAGCTGCATGAAATATATTTGACACTGAGGGACATGCATCGTGACTCTATAACACCAAAATCAACCATCGGTCACCATGCACTTGTTTTCTTTCTACCTATGCACACAAAAGCAATACATGCAGCATATGCAGCGCGGCTGAGTGAAAATGAGTGCAAGCATTTGCTTGATCTCTTGCACAAAATCTATCTCCCAGATTTCTTCTCTCCCAGCCCAACCCCCATCTCTGTAGAGTCGACTACCAAAGGTGGTTATAGTCTAAGCAATAGAAACTGGGGATTCAATTTTTCCACAGCAGAACTTTTAGAGGCTGAAATCGAGTTTCAACTACAGCAGGAGTACTTGCCTTACATGAGTTTTGAGAAGGGGGTGATTAGAATCCCTCCGTTGGTGATGGATGAGACAACAAATTCAATGATGAGGAATTTAATTGCTTATGAGCAATGTGGTTTCGTAAAAAATTACTTTACTTCATTTGCCATCGTCCTGAAAAGTTTGATACGATCGTTGGAGGATATTAAACTACTTCGGCAAAGAAAGATCATAAGTTCCACTTGGACTGCTGATGAAGAATGTTTGATTCATTTCAACACTATGATGGATGAAGTTGTTGTGAAGGAATTTTACTTTTTCCACTTCTTGTGCACAAAAGTGAATGCGAAAGCCGAAGAGTTTGGGTTTCGTAGATTCATGAAGAACCTTTATCGCACTTATTTCTCAACAGCATGGAGTCTGATATCACTCATTGCTGCATCTTGCCTTTTCGTTCTCACTGTCATTCAAACATACTTCTCCATTCGCCAAAGTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

454

Amino Acids

53.08

Weight (kDa)

6.58

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 35 - 441 4.7e-89 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022987)

Species Orthologous Gene IDs
rosa_laevigata RLG00000030593
rosa_multiflora Rmu_sc0003008.1_g000015
rosa_samantha Rh1DG018900
rosa_wichuraiana Rw1G001540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 788
AccII CGCG 1 cut(s) 688
AciI CCGC 2 cut(s) 362, 688
AclWI GGATC 4 cut(s) 74, 458, 494, 526
AcsI RAATTY 3 cut(s) 961, 976, 1172
AcuI CTGAAG 1 cut(s) 77
AfaI GTAC 1 cut(s) 893
AfiI CCNNNNNNNGG 1 cut(s) 940
AflIII ACRYGT 1 cut(s) 348
AgsI TTSAA 6 cut(s) 203, 835, 878, 966, 1141, 1334
AhlI ACTAGT 1 cut(s) 26
AjnI CCWGG 1 cut(s) 451
AleI CACNNNNGTG 1 cut(s) 1202
AluBI AGCT 5 cut(s) 55, 217, 406, 497, 556
AluI AGCT 5 cut(s) 55, 217, 406, 497, 556
Alw21I GWGCWC 1 cut(s) 1199
Alw26I GTCTC 2 cut(s) 169, 947
Alw44I GTGCAC 1 cut(s) 1195
AlwI GGATC 4 cut(s) 74, 458, 494, 526
ApaLI GTGCAC 1 cut(s) 1195
ApeKI GCWGC 4 cut(s) 556, 674, 683, 1301
ApoI RAATTY 3 cut(s) 961, 976, 1172
AseI ATTAAT 1 cut(s) 297
AspLEI GCGC 2 cut(s) 287, 688
AsuHPI GGTGA 4 cut(s) 467, 617, 934, 955
AsuII TTCGAA 2 cut(s) 115, 127
AxyI CCTNAGG 1 cut(s) 413
BaeGI GKGCMC 1 cut(s) 1199
BaeI ACNNNNGTAYC 2 cut(s) 1043, 1076
BarI GAAGNNNNNNTAC 2 cut(s) 1160, 1192
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 1199
BbvI GCAGC 4 cut(s) 543, 686, 695, 1288
BccI CCATC 9 cut(s) 11, 78, 326, 380, 626, 782, 940, 1040, 1144
BciT130I CCWGG 1 cut(s) 453
BclI TGATCA 2 cut(s) 45, 457
BcoDI GTCTC 2 cut(s) 169, 947
BcuI ACTAGT 1 cut(s) 26
BfaI CTAG 1 cut(s) 27
BfmI CTRYAG 2 cut(s) 780, 881
BisI GCNGC 5 cut(s) 557, 675, 684, 689, 1302
BlsI GCNGC 5 cut(s) 558, 676, 685, 690, 1303
BmcAI AGTACT 1 cut(s) 893
Bme1390I CCNGG 1 cut(s) 453
BmrFI CCNGG 1 cut(s) 453
BmrI ACTGGG 1 cut(s) 835
BmsI GCATC 4 cut(s) 133, 348, 598, 1313
BmuI ACTGGG 1 cut(s) 835
BpiI GAAGAC 1 cut(s) 192
Bpu14I TTCGAA 2 cut(s) 115, 127
BpuEI CTTGAG 1 cut(s) 118
BsaBI GATNNNNATC 2 cut(s) 462, 929
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 940
Bse1I ACTGG 1 cut(s) 830
Bse21I CCTNAGG 1 cut(s) 413
Bse3DI GCAATG 3 cut(s) 242, 1001, 1296
Bse8I GATNNNNATC 2 cut(s) 462, 929
BseBI CCWGG 1 cut(s) 453
BseGI GGATG 4 cut(s) 148, 489, 955, 1159
BseJI GATNNNNATC 2 cut(s) 462, 929
BseLI CCNNNNNNNGG 1 cut(s) 940
BseMI GCAATG 3 cut(s) 242, 1001, 1296
BseMII CTCAG 3 cut(s) 84, 569, 683
BseNI ACTGG 1 cut(s) 830
BseSI GKGCMC 1 cut(s) 1199
BseXI GCAGC 4 cut(s) 543, 686, 695, 1288
BseYI CCCAGC 2 cut(s) 493, 761
Bsh1236I CGCG 1 cut(s) 688
Bsh1285I CGRYCG 1 cut(s) 1058
BsiEI CGRYCG 1 cut(s) 1058
BsiHKAI GWGCWC 1 cut(s) 1199
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 1 cut(s) 940
BsmAI GTCTC 2 cut(s) 169, 947
BsmBI CGTCTC 1 cut(s) 169
BsmFI GGGAC 1 cut(s) 596
BsmI GAATGC 2 cut(s) 275, 1213
Bsp119I TTCGAA 2 cut(s) 115, 127
Bsp1286I GDGCHC 1 cut(s) 1199
BspACI CCGC 2 cut(s) 362, 688
BspCNI CTCAG 3 cut(s) 83, 570, 684
BspFNI CGCG 1 cut(s) 688
BspHI TCATGA 2 cut(s) 460, 1242
BspPI GGATC 4 cut(s) 74, 458, 494, 526
BspQI GCTCTTC 1 cut(s) 504
BspT104I TTCGAA 2 cut(s) 115, 127
BsrDI GCAATG 3 cut(s) 242, 1001, 1296
BsrI ACTGG 1 cut(s) 830
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 1 cut(s) 1327
Bst6I CTCTTC 3 cut(s) 273, 504, 1215
BstAPI GCANNNNNTGC 3 cut(s) 232, 671, 680
BstBI TTCGAA 2 cut(s) 115, 127
BstC8I GCNNGC 3 cut(s) 252, 325, 710
BstDEI CTNAG 5 cut(s) 70, 413, 578, 692, 812
BstEII GGTNACC 1 cut(s) 623
BstF5I GGATG 4 cut(s) 148, 489, 955, 1159
BstFNI CGCG 1 cut(s) 688
BstHHI GCGC 2 cut(s) 287, 688
BstMAI GTCTC 2 cut(s) 169, 947
BstMCI CGRYCG 1 cut(s) 1058
BstMWI GCNNNNNNNGC 6 cut(s) 223, 232, 671, 680, 683, 1359
BstNI CCWGG 1 cut(s) 453
BstNSI RCATGY 3 cut(s) 352, 589, 674
BstPI GGTNACC 1 cut(s) 623
BstSCI CCNGG 1 cut(s) 451
BstSFI CTRYAG 2 cut(s) 780, 881
BstSLI GKGCMC 1 cut(s) 1199
BstUI CGCG 1 cut(s) 688
BstV1I GCAGC 4 cut(s) 543, 686, 695, 1288
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 2 cut(s) 66, 531
BstYI RGATCY 2 cut(s) 66, 531
Bsu36I CCTNAGG 1 cut(s) 413
BtsCI GGATG 4 cut(s) 148, 489, 955, 1159
BtsIMutI CAGTG 2 cut(s) 575, 1323
Cac8I GCNNGC 3 cut(s) 252, 325, 710
CciI TCATGA 2 cut(s) 460, 1242
CfoI GCGC 2 cut(s) 287, 688
Csp6I GTAC 1 cut(s) 892
CviJI RGCY 9 cut(s) 55, 217, 406, 497, 556, 691, 765, 863, 1217
CviKI_1 RGCY 9 cut(s) 55, 217, 406, 497, 556, 691, 765, 863, 1217
CviQI GTAC 1 cut(s) 892
DdeI CTNAG 5 cut(s) 70, 413, 578, 692, 812
Eam1104I CTCTTC 3 cut(s) 273, 504, 1215
EarI CTCTTC 3 cut(s) 273, 504, 1215
Eco32I GATATC 1 cut(s) 1290
Eco57I CTGAAG 1 cut(s) 77
Eco81I CCTNAGG 1 cut(s) 413
Eco91I GGTNACC 1 cut(s) 623
EcoO65I GGTNACC 1 cut(s) 623
EcoRII CCWGG 1 cut(s) 451
EcoRV GATATC 1 cut(s) 1290
EcoT22I ATGCAT 1 cut(s) 591
Esp3I CGTCTC 1 cut(s) 169
FalI AAGNNNNNCTT 2 cut(s) 1090, 1122
FaqI GGGAC 1 cut(s) 596
FauNDI CATATG 1 cut(s) 679
FbaI TGATCA 2 cut(s) 45, 457
FblI GTMKAC 1 cut(s) 788
Fnu4HI GCNGC 5 cut(s) 557, 675, 684, 689, 1302
FokI GGATG 4 cut(s) 155, 476, 962, 1166
Fsp4HI GCNGC 5 cut(s) 557, 675, 684, 689, 1302
FspBI CTAG 1 cut(s) 27
GlaI GCGC 2 cut(s) 286, 687
GluI GCNGC 5 cut(s) 557, 675, 684, 689, 1302
GsaI CCCAGC 2 cut(s) 497, 765
HhaI GCGC 2 cut(s) 287, 688
Hin6I GCGC 2 cut(s) 285, 686
HinP1I GCGC 2 cut(s) 285, 686
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HinfI GANTC 9 cut(s) 8, 448, 596, 785, 831, 930, 1132, 1239, 1282
HphI GGTGA 4 cut(s) 467, 617, 934, 955
Hpy166II GTNNAC 3 cut(s) 305, 789, 1197
Hpy188I TCNGA 4 cut(s) 73, 91, 173, 1287
Hpy188III TCNNGA 6 cut(s) 182, 266, 461, 593, 1039, 1243
Hpy8I GTNNAC 3 cut(s) 305, 789, 1197
HpyAV CCTTC 4 cut(s) 130, 197, 910, 1162
HpyCH4III ACNGT 1 cut(s) 1327
HpyF10VI GCNNNNNNNGC 6 cut(s) 223, 232, 671, 680, 683, 1359
HpyF3I CTNAG 5 cut(s) 70, 413, 578, 692, 812
HspAI GCGC 2 cut(s) 285, 686
Ksp22I TGATCA 2 cut(s) 45, 457
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 3 cut(s) 52, 384, 403
Lsp1109I GCAGC 4 cut(s) 543, 686, 695, 1288
LweI GCATC 4 cut(s) 133, 348, 598, 1313
MaeI CTAG 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 593, 623
MboII GAAGA 7 cut(s) 197, 290, 491, 745, 1133, 1232, 1258
MflI RGATCY 2 cut(s) 66, 531
MhlI GDGCHC 1 cut(s) 1199
MluCI AATT 8 cut(s) 374, 468, 835, 961, 976, 981, 1012, 1172
MlyI GAGTC 4 cut(s) 457, 590, 794, 1291
MmeI TCCRAC 1 cut(s) 1041
Mph1103I ATGCAT 1 cut(s) 591
MseI TTAA 5 cut(s) 219, 297, 552, 980, 1071
MslI CAYNNNNRTG 2 cut(s) 1148, 1202
MspR9I CCNGG 1 cut(s) 453
Mva1269I GAATGC 2 cut(s) 275, 1213
MvaI CCWGG 1 cut(s) 453
MvnI CGCG 1 cut(s) 688
MwoI GCNNNNNNNGC 6 cut(s) 223, 232, 671, 680, 683, 1359
NdeI CATATG 1 cut(s) 679
NmuCI GTSAC 2 cut(s) 593, 623
NsiI ATGCAT 1 cut(s) 591
NspI RCATGY 3 cut(s) 352, 589, 674
NspV TTCGAA 2 cut(s) 115, 127
OliI CACNNNNGTG 1 cut(s) 1202
PagI TCATGA 2 cut(s) 460, 1242
PciI ACATGT 1 cut(s) 348
PciSI GCTCTTC 1 cut(s) 504
PctI GAATGC 2 cut(s) 275, 1213
PfeI GAWTC 5 cut(s) 8, 831, 930, 1132, 1239
PkrI GCNGC 5 cut(s) 558, 676, 685, 690, 1303
Ple19I CGATCG 1 cut(s) 1058
PleI GAGTC 4 cut(s) 456, 590, 793, 1290
PpsI GAGTC 4 cut(s) 456, 590, 793, 1290
PscI ACATGT 1 cut(s) 348
PshBI ATTAAT 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 451
PspEI GGTNACC 1 cut(s) 623
PspFI CCCAGC 2 cut(s) 493, 761
PspGI CCWGG 1 cut(s) 451
PsuI RGATCY 2 cut(s) 66, 531
PvuI CGATCG 1 cut(s) 1058
RsaI GTAC 1 cut(s) 893
RsaNI GTAC 1 cut(s) 892
RseI CAYNNNNRTG 2 cut(s) 1148, 1202
SalI GTCGAC 1 cut(s) 787
SapI GCTCTTC 1 cut(s) 504
SaqAI TTAA 5 cut(s) 219, 297, 552, 980, 1071
SatI GCNGC 5 cut(s) 557, 675, 684, 689, 1302
ScaI AGTACT 1 cut(s) 893
SchI GAGTC 4 cut(s) 457, 590, 794, 1291
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 1 cut(s) 1199
SfaNI GCATC 4 cut(s) 133, 348, 598, 1313
SfcI CTRYAG 2 cut(s) 780, 881
SfuI TTCGAA 2 cut(s) 115, 127
SmiMI CAYNNNNRTG 2 cut(s) 1148, 1202
SmlI CTYRAG 1 cut(s) 97
SmoI CTYRAG 1 cut(s) 97
SpeI ACTAGT 1 cut(s) 26
Sse9I AATT 8 cut(s) 374, 468, 835, 961, 976, 981, 1012, 1172
SsiI CCGC 2 cut(s) 362, 688
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 1 cut(s) 451
TaaI ACNGT 1 cut(s) 1327
TaqI TCGA 5 cut(s) 115, 127, 522, 788, 870
TaqII GACCGA 1 cut(s) 611
TasI AATT 8 cut(s) 374, 468, 835, 961, 976, 981, 1012, 1172
TatI WGTACW 1 cut(s) 891
TauI GCSGC 1 cut(s) 691
TfiI GAWTC 5 cut(s) 8, 831, 930, 1132, 1239
Tru1I TTAA 5 cut(s) 219, 297, 552, 980, 1071
Tru9I TTAA 5 cut(s) 219, 297, 552, 980, 1071
TscAI CASTG 2 cut(s) 582, 1330
TseFI GTSAC 2 cut(s) 593, 623
TseI GCWGC 4 cut(s) 556, 674, 683, 1301
Tsp45I GTSAC 2 cut(s) 593, 623
TspDTI ATGAA 9 cut(s) 210, 384, 576, 1014, 1124, 1134, 1170, 1231, 1259
TspGWI ACGGA 1 cut(s) 927
TspRI CASTG 2 cut(s) 582, 1330
VneI GTGCAC 1 cut(s) 1195
VspI ATTAAT 1 cut(s) 297
XapI RAATTY 3 cut(s) 961, 976, 1172
XceI RCATGY 3 cut(s) 352, 589, 674
XmiI GTMKAC 1 cut(s) 788
XspI CTAG 1 cut(s) 27
ZrmI AGTACT 1 cut(s) 893
Zsp2I ATGCAT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.