RLG00000031227
MADS Family

MADS-box transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017997
Physical Location & Seq
Forward (+)
3172325 .. 3172633
309 bp
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UTR
Exon/CDS
Intron
RLM00000031227

Sequence Viewer

Length: 309 bp
ATGGGTACCGGAAAGAAGAAAATTGACATAGAGCCGATCACCGACTACCAATCTCGCATGGTGGCCTTCACCAGGAGACGAACAGGTCTCTTCAAAAAAGCCCAGAAGCTCGAAGAAATGTCTGGTTCATCCATCGCCATTGCTGTGATCTCCGAGACCGGTCAACCTTACCTGCATGGCTCTCCTTCCTTGCTTGAACGAGTTCTTGCCTCCAAAGCCTCTTCCTCCACAGTTACTGGCTCTTCCACCACCACAGCTGACACTGGCTCTTCCACCACCACCGCCACTACAGGATCTTTAGAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04111 map04011 map04111
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

103

Amino Acids

10.76

Weight (kDa)

9.69

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 12 - 57 9.2e-14 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017997)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g02880
rosa_chinensis RchiOBHm_Chr5g0004451
rosa_laevigata RLG00000031227
rosa_multiflora Rmu_sc0000433.1_g000010 Rmu_sc0002453.1_g000004
rosa_roxburghii Rroxscaffold_1G00071100
rosa_rugosa Rorug04G0411700
rosa_samantha Rh5AG039100 Rh5BG038100 Rh5CG042300 Rh5DG037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 84
Acc36I ACCTGC 1 cut(s) 180
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 5
AciI CCGC 1 cut(s) 282
AclWI GGATC 1 cut(s) 301
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 72
AgeI ACCGGT 1 cut(s) 158
AgsI TTSAA 2 cut(s) 94, 197
AjnI CCWGG 1 cut(s) 71
AluBI AGCT 2 cut(s) 109, 257
AluI AGCT 2 cut(s) 109, 257
Alw26I GTCTC 3 cut(s) 70, 92, 149
AlwI GGATC 1 cut(s) 301
AlwNI CAGNNNCTG 1 cut(s) 236
AoxI GGCC 1 cut(s) 63
AsiGI ACCGGT 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 201
Asp718I GGTACC 1 cut(s) 5
AsuHPI GGTGA 2 cut(s) 31, 61
BanI GGYRCC 1 cut(s) 5
BccI CCATC 1 cut(s) 140
BciT130I CCWGG 1 cut(s) 73
BcoDI GTCTC 3 cut(s) 70, 92, 149
BfmI CTRYAG 1 cut(s) 288
BfuAI ACCTGC 1 cut(s) 180
Bme1390I CCNGG 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 7
BmrFI CCNGG 1 cut(s) 73
BsaI GGTCTC 2 cut(s) 92, 149
BsaWI WCCGGW 2 cut(s) 8, 158
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 2 cut(s) 241, 268
Bse3DI GCAATG 1 cut(s) 138
BseBI CCWGG 1 cut(s) 73
BseGI GGATG 1 cut(s) 128
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMI GCAATG 1 cut(s) 138
BseNI ACTGG 2 cut(s) 241, 268
BshFI GGCC 1 cut(s) 65
BshNI GGYRCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 158
BsiSI CCGG 2 cut(s) 9, 159
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 3 cut(s) 70, 92, 149
BsmBI CGTCTC 1 cut(s) 70
BsnI GGCC 1 cut(s) 65
Bso31I GGTCTC 2 cut(s) 92, 149
Bsp143I GATC 3 cut(s) 36, 147, 293
BspACI CCGC 1 cut(s) 282
BspANI GGCC 1 cut(s) 65
BspLI GGNNCC 1 cut(s) 7
BspMI ACCTGC 1 cut(s) 180
BspPI GGATC 1 cut(s) 301
BspQI GCTCTTC 2 cut(s) 247, 274
BspT107I GGYRCC 1 cut(s) 5
BspTNI GGTCTC 2 cut(s) 92, 149
BsrDI GCAATG 1 cut(s) 138
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 2 cut(s) 241, 268
BssAI RCCGGY 1 cut(s) 158
BssMI GATC 3 cut(s) 36, 147, 293
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 4 cut(s) 95, 226, 247, 274
BstENI CCTNNNNNAGG 1 cut(s) 70
BstF5I GGATG 1 cut(s) 128
BstKTI GATC 3 cut(s) 39, 150, 296
BstMAI GTCTC 3 cut(s) 70, 92, 149
BstMBI GATC 3 cut(s) 36, 147, 293
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstNI CCWGG 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 71
BstSFI CTRYAG 1 cut(s) 288
BstX2I RGATCY 1 cut(s) 293
BstYI RGATCY 1 cut(s) 293
BsuRI GGCC 1 cut(s) 65
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 261
BveI ACCTGC 1 cut(s) 180
CaiI CAGNNNCTG 1 cut(s) 236
Cfr10I RCCGGY 1 cut(s) 158
Csp6I GTAC 1 cut(s) 6
CspAI ACCGGT 1 cut(s) 158
CviAII CATG 2 cut(s) 58, 176
CviJI RGCY 9 cut(s) 34, 65, 101, 109, 180, 218, 240, 257, 267
CviKI_1 RGCY 9 cut(s) 34, 65, 101, 109, 180, 218, 240, 257, 267
CviQI GTAC 1 cut(s) 6
DpnI GATC 3 cut(s) 38, 149, 295
DpnII GATC 3 cut(s) 36, 147, 293
DrdI GACNNNNNNGTC 1 cut(s) 84
DseDI GACNNNNNNGTC 1 cut(s) 84
Eam1104I CTCTTC 4 cut(s) 95, 226, 247, 274
EarI CTCTTC 4 cut(s) 95, 226, 247, 274
Eco31I GGTCTC 2 cut(s) 92, 149
EcoNI CCTNNNNNAGG 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 71
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 2 cut(s) 61, 179
FaiI YATR 3 cut(s) 29, 59, 177
FatI CATG 2 cut(s) 57, 175
FokI GGATG 1 cut(s) 115
HaeIII GGCC 1 cut(s) 65
HapII CCGG 2 cut(s) 9, 159
Hin1II CATG 2 cut(s) 61, 179
HincII GTYRAC 1 cut(s) 164
HindII GTYRAC 1 cut(s) 164
HpaII CCGG 2 cut(s) 9, 159
HphI GGTGA 2 cut(s) 31, 61
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 1 cut(s) 154
Hpy8I GTNNAC 1 cut(s) 164
HpyAV CCTTC 2 cut(s) 76, 195
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4V TGCA 1 cut(s) 175
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
Hsp92II CATG 2 cut(s) 61, 179
KpnI GGTACC 1 cut(s) 9
Kzo9I GATC 3 cut(s) 36, 147, 293
LguI GCTCTTC 2 cut(s) 247, 274
MaeIII GTNAC 1 cut(s) 232
MalI GATC 3 cut(s) 38, 149, 295
MboI GATC 3 cut(s) 36, 147, 293
MboII GAAGA 6 cut(s) 28, 82, 125, 213, 234, 261
MflI RGATCY 1 cut(s) 293
MluCI AATT 1 cut(s) 21
MnlI CCTC 3 cut(s) 220, 229, 235
MroXI GAANNNNTTC 1 cut(s) 201
MslI CAYNNNNRTG 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 2 cut(s) 9, 159
MspR9I CCNGG 1 cut(s) 73
MvaI CCWGG 1 cut(s) 73
MwoI GCNNNNNNNGC 1 cut(s) 215
NdeII GATC 3 cut(s) 36, 147, 293
NlaIII CATG 2 cut(s) 61, 179
NlaIV GGNNCC 1 cut(s) 7
PciSI GCTCTTC 2 cut(s) 247, 274
PdmI GAANNNNTTC 1 cut(s) 201
PinAI ACCGGT 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 7
PstNI CAGNNNCTG 1 cut(s) 236
PsuI RGATCY 1 cut(s) 293
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 143
SapI GCTCTTC 2 cut(s) 247, 274
Sau3AI GATC 3 cut(s) 36, 147, 293
ScrFI CCNGG 1 cut(s) 73
SetI ASST 5 cut(s) 88, 111, 169, 174, 259
SfcI CTRYAG 1 cut(s) 288
SmiMI CAYNNNNRTG 1 cut(s) 143
Sse9I AATT 1 cut(s) 21
SsiI CCGC 1 cut(s) 282
StyD4I CCNGG 1 cut(s) 71
TaaI ACNGT 1 cut(s) 232
TaqI TCGA 1 cut(s) 111
TasI AATT 1 cut(s) 21
TscAI CASTG 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 117
TspRI CASTG 1 cut(s) 268
XagI CCTNNNNNAGG 1 cut(s) 70
XmnI GAANNNNTTC 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.