RLG00000032252

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0030941
Physical Location & Seq
Forward (+)
12614347 .. 12615760
1414 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032252

Sequence Viewer

Length: 849 bp
ATGGCAAACCAAATTTTGGTATTGACAGTCCTAGCCATGACATGTACTCTGGTCATTTCCTTTGAGCCTAGTCCACTGCAAGATTTCTGCGTAGCTGACACTAATAGCTCAGTACCAAGAGTAAATGGCCTACCTTGCTTAGACTCCAAGTTAGCAAAAGCTGACCATTTCTTCTTCGGAGGGCTTCACCTTGCCGGAAACACCTCAAACCCTCTTGGAGTTAAAATCACTTCAGTCAATGTGGCTCAAATTCCAGGACTCAACACTCTTGGCATCTCACTTGCTCGTATTGACTATGCACCGTGGGGCGTTACCCCTCCTCACACTCATCCTCGTGCCACTGAAATTCTAACAGTCTTGGAAGGCAGTGTTGCTGTTGGGTTTGTGACATCTAACCCCGACAACAAGCTCATCTCTAAGGTGCTCAACAAGGGTGATGTGTTTGTTTTCCCTGTTGGACTAGTTCACTATCAGCAAAATGTGGGTAATGGAACTGCCGTTTCACTCTCTTCTCTGAGCAGCCAAAACCCTGGAGTCATCACTATTGCTAATTCAGTTTTTGGAGCGAATCCCTCAATTTCGGATGATGTTCTAGCCAAGGCTTTTCAGCCGGACTGCCAGATGCCGAGCCACGACGCCTTCAGCACCATCTTCTCCGAGATCGGTGCCGGCAAGCATGTCCCAAGCGTAATCTTCGCCGAACTCGAGCCCACCGTCAAAGCCCACCATCTTCTTCTCCGAGATCGGTGCCGGCAAGCATATCCCAGGCGTAATCTTCGCCGACCTCGAGCCCACCGTCAAAGCCCACCATCTTATTCTCCGAGATCGGTGCCGGCAAGCATGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

283

Amino Acids

30.18

Weight (kDa)

8.87

Isoelectric Point (pI)

43.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 65 - 203 1.3e-47 Cupin
Cupin_2 PF07883 97 - 168 5.4e-09 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0030941)

Species Orthologous Gene IDs
rosa_laevigata RLG00000032252
rosa_multiflora Rmu_sc0000469.1_g000004

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 665, 747, 829
AccB7I CCANNNNNTGG 1 cut(s) 16
AcsI RAATTY 3 cut(s) 12, 249, 345
AcuI CTGAAG 2 cut(s) 216, 625
AcyI GRCGYC 1 cut(s) 636
AfaI GTAC 2 cut(s) 46, 114
AfiI CCNNNNNNNGG 1 cut(s) 16
AflIII ACRYGT 1 cut(s) 41
AhlI ACTAGT 1 cut(s) 460
AjnI CCWGG 3 cut(s) 253, 529, 764
AluBI AGCT 4 cut(s) 95, 108, 161, 409
AluI AGCT 4 cut(s) 95, 108, 161, 409
Alw21I GWGCWC 1 cut(s) 426
Ama87I CYCGRG 2 cut(s) 704, 786
AoxI GGCC 1 cut(s) 127
ApeKI GCWGC 1 cut(s) 519
ApoI RAATTY 3 cut(s) 12, 249, 345
AsuHPI GGTGA 2 cut(s) 179, 446
AvaI CYCGRG 2 cut(s) 704, 786
BanI GGYRCC 3 cut(s) 665, 747, 829
BanII GRGCYC 2 cut(s) 711, 793
BauI CACGAG 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 1 cut(s) 531
BccI CCATC 3 cut(s) 656, 735, 817
BceAI ACGGC 1 cut(s) 482
BcgI CGANNNNNNTGC 6 cut(s) 647, 681, 729, 763, 811, 845
BciT130I CCWGG 3 cut(s) 255, 531, 766
BcuI ACTAGT 1 cut(s) 460
BfaI CTAG 5 cut(s) 32, 69, 461, 593, 847
BisI GCNGC 1 cut(s) 520
BlsI GCNGC 1 cut(s) 521
Bme1390I CCNGG 3 cut(s) 255, 531, 766
BmeT110I CYCGRG 2 cut(s) 704, 786
BmiI GGNNCC 3 cut(s) 667, 749, 831
BmrFI CCNGG 3 cut(s) 255, 531, 766
BmsI GCATC 2 cut(s) 282, 612
BpmI CTGGAG 1 cut(s) 552
BsaHI GRCGYC 1 cut(s) 636
BsaJI CCNNGG 4 cut(s) 302, 529, 597, 764
Bsc4I CCNNNNNNNGG 1 cut(s) 16
Bse118I RCCGGY 3 cut(s) 668, 750, 832
BseBI CCWGG 3 cut(s) 255, 531, 766
BseDI CCNNGG 4 cut(s) 302, 529, 597, 764
BseGI GGATG 2 cut(s) 328, 589
BseLI CCNNNNNNNGG 1 cut(s) 16
BseMII CTCAG 2 cut(s) 123, 506
BseRI GAGGAG 1 cut(s) 309
BseXI GCAGC 1 cut(s) 531
BshFI GGCC 1 cut(s) 129
BshNI GGYRCC 3 cut(s) 665, 747, 829
BsiHKAI GWGCWC 1 cut(s) 426
BsiHKCI CYCGRG 2 cut(s) 704, 786
BsiSI CCGG 5 cut(s) 195, 611, 669, 751, 833
BslFI GGGAC 1 cut(s) 665
BslI CCNNNNNNNGG 1 cut(s) 16
BsmFI GGGAC 1 cut(s) 665
BsnI GGCC 1 cut(s) 129
BsoBI CYCGRG 2 cut(s) 704, 786
Bsp1286I GDGCHC 3 cut(s) 426, 711, 793
Bsp143I GATC 3 cut(s) 660, 742, 824
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 2 cut(s) 122, 507
BspLI GGNNCC 3 cut(s) 667, 749, 831
BspT107I GGYRCC 3 cut(s) 665, 747, 829
BsrFI RCCGGY 3 cut(s) 668, 750, 832
BssAI RCCGGY 3 cut(s) 668, 750, 832
BssECI CCNNGG 4 cut(s) 302, 529, 597, 764
BssMI GATC 3 cut(s) 660, 742, 824
BssNI GRCGYC 1 cut(s) 636
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 597
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 3 cut(s) 255, 531, 766
Bst4CI ACNGT 5 cut(s) 28, 303, 355, 715, 797
Bst6I CTCTTC 1 cut(s) 514
BstACI GRCGYC 1 cut(s) 636
BstC8I GCNNGC 6 cut(s) 670, 674, 752, 756, 834, 838
BstDEI CTNAG 4 cut(s) 109, 139, 417, 515
BstDSI CCRYGG 1 cut(s) 302
BstF5I GGATG 2 cut(s) 328, 589
BstKTI GATC 3 cut(s) 663, 745, 827
BstMBI GATC 3 cut(s) 660, 742, 824
BstMWI GCNNNNNNNGC 1 cut(s) 135
BstNI CCWGG 3 cut(s) 255, 531, 766
BstNSI RCATGY 3 cut(s) 45, 680, 844
BstSCI CCNGG 3 cut(s) 253, 529, 764
BstV1I GCAGC 1 cut(s) 531
BstXI CCANNNNNNTGG 1 cut(s) 530
BsuRI GGCC 1 cut(s) 129
BtgI CCRYGG 1 cut(s) 302
BtsCI GGATG 2 cut(s) 328, 589
BtsI GCAGTG 2 cut(s) 74, 373
BtsIMutI CAGTG 3 cut(s) 74, 339, 373
Cac8I GCNNGC 6 cut(s) 670, 674, 752, 756, 834, 838
Cfr10I RCCGGY 3 cut(s) 668, 750, 832
CseI GACGC 1 cut(s) 644
Csp6I GTAC 2 cut(s) 45, 113
CviAII CATG 4 cut(s) 37, 42, 677, 841
CviQI GTAC 2 cut(s) 45, 113
DdeI CTNAG 4 cut(s) 109, 139, 417, 515
DpnI GATC 3 cut(s) 662, 744, 826
DpnII GATC 3 cut(s) 660, 742, 824
Eam1104I CTCTTC 1 cut(s) 514
EarI CTCTTC 1 cut(s) 514
Eco130I CCWWGG 1 cut(s) 597
Eco24I GRGCYC 2 cut(s) 711, 793
Eco57I CTGAAG 2 cut(s) 216, 625
Eco88I CYCGRG 2 cut(s) 704, 786
EcoRII CCWGG 3 cut(s) 253, 529, 764
EcoT14I CCWWGG 1 cut(s) 597
EcoT38I GRGCYC 2 cut(s) 711, 793
ErhI CCWWGG 1 cut(s) 597
FaeI CATG 4 cut(s) 40, 45, 680, 844
FaiI YATR 6 cut(s) 38, 43, 297, 678, 760, 842
FaqI GGGAC 1 cut(s) 665
FatI CATG 4 cut(s) 36, 41, 676, 840
Fnu4HI GCNGC 1 cut(s) 520
FokI GGATG 2 cut(s) 315, 596
FriOI GRGCYC 2 cut(s) 711, 793
Fsp4HI GCNGC 1 cut(s) 520
FspBI CTAG 5 cut(s) 32, 69, 461, 593, 847
GluI GCNGC 1 cut(s) 520
GsuI CTGGAG 1 cut(s) 552
HaeIII GGCC 1 cut(s) 129
HapII CCGG 5 cut(s) 195, 611, 669, 751, 833
HgaI GACGC 1 cut(s) 644
Hin1I GRCGYC 1 cut(s) 636
Hin1II CATG 4 cut(s) 40, 45, 680, 844
HinfI GANTC 4 cut(s) 143, 258, 534, 568
HpaII CCGG 5 cut(s) 195, 611, 669, 751, 833
HphI GGTGA 2 cut(s) 179, 446
Hpy166II GTNNAC 2 cut(s) 74, 466
Hpy188I TCNGA 6 cut(s) 179, 516, 583, 658, 740, 822
Hpy8I GTNNAC 2 cut(s) 74, 466
Hpy99I CGWCG 1 cut(s) 638
HpyAV CCTTC 2 cut(s) 356, 649
HpyCH4III ACNGT 5 cut(s) 28, 303, 355, 715, 797
HpyCH4V TGCA 2 cut(s) 79, 299
HpyF10VI GCNNNNNNNGC 1 cut(s) 135
HpyF3I CTNAG 4 cut(s) 109, 139, 417, 515
Hsp92I GRCGYC 1 cut(s) 636
Hsp92II CATG 4 cut(s) 40, 45, 680, 844
KroI GCCGGC 3 cut(s) 668, 750, 832
KroNI GCCGGC 3 cut(s) 670, 752, 834
Kzo9I GATC 3 cut(s) 660, 742, 824
LmnI GCTCC 1 cut(s) 563
Lsp1109I GCAGC 1 cut(s) 531
LweI GCATC 2 cut(s) 282, 612
MaeI CTAG 5 cut(s) 32, 69, 461, 593, 847
MaeIII GTNAC 2 cut(s) 310, 385
MalI GATC 3 cut(s) 662, 744, 826
MboI GATC 3 cut(s) 660, 742, 824
MboII GAAGA 8 cut(s) 163, 166, 501, 643, 685, 722, 725, 767
MhlI GDGCHC 3 cut(s) 426, 711, 793
MluCI AATT 5 cut(s) 12, 249, 345, 550, 576
MlyI GAGTC 3 cut(s) 137, 252, 543
MmeI TCCRAC 1 cut(s) 436
MnlI CCTC 8 cut(s) 173, 214, 222, 327, 330, 342, 583, 795
MroNI GCCGGC 3 cut(s) 668, 750, 832
MseI TTAA 1 cut(s) 222
MslI CAYNNNNRTG 1 cut(s) 333
MspI CCGG 5 cut(s) 195, 611, 669, 751, 833
MspR9I CCNGG 3 cut(s) 255, 531, 766
MvaI CCWGG 3 cut(s) 255, 531, 766
MwoI GCNNNNNNNGC 1 cut(s) 135
NaeI GCCGGC 3 cut(s) 670, 752, 834
NdeII GATC 3 cut(s) 660, 742, 824
NgoMIV GCCGGC 3 cut(s) 668, 750, 832
NlaIII CATG 4 cut(s) 40, 45, 680, 844
NlaIV GGNNCC 3 cut(s) 667, 749, 831
NmeAIII GCCGAG 1 cut(s) 651
NmuCI GTSAC 1 cut(s) 385
NspI RCATGY 3 cut(s) 45, 680, 844
PaeR7I CTCGAG 2 cut(s) 704, 786
PciI ACATGT 1 cut(s) 41
PcsI WCGNNNNNNNCGW 4 cut(s) 702, 711, 784, 793
PdiI GCCGGC 3 cut(s) 670, 752, 834
PfeI GAWTC 1 cut(s) 568
PflMI CCANNNNNTGG 1 cut(s) 16
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 1 cut(s) 521
PleI GAGTC 3 cut(s) 137, 252, 542
PpsI GAGTC 3 cut(s) 137, 252, 542
PscI ACATGT 1 cut(s) 41
Psp6I CCWGG 3 cut(s) 253, 529, 764
PspGI CCWGG 3 cut(s) 253, 529, 764
PspN4I GGNNCC 3 cut(s) 667, 749, 831
PspXI VCTCGAGB 2 cut(s) 704, 786
RsaI GTAC 2 cut(s) 46, 114
RsaNI GTAC 2 cut(s) 45, 113
RseI CAYNNNNRTG 1 cut(s) 333
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 1 cut(s) 520
Sau3AI GATC 3 cut(s) 660, 742, 824
SchI GAGTC 3 cut(s) 137, 252, 543
ScrFI CCNGG 3 cut(s) 255, 531, 766
SduI GDGCHC 3 cut(s) 426, 711, 793
SetI ASST 9 cut(s) 97, 110, 136, 163, 192, 206, 411, 423, 787
SfaNI GCATC 2 cut(s) 282, 612
Sfr274I CTCGAG 2 cut(s) 704, 786
SlaI CTCGAG 2 cut(s) 704, 786
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 2 cut(s) 704, 786
SmoI CTYRAG 2 cut(s) 704, 786
SpeI ACTAGT 1 cut(s) 460
Sse9I AATT 5 cut(s) 12, 249, 345, 550, 576
SspMI CTAG 5 cut(s) 32, 69, 461, 593, 847
StyD4I CCNGG 3 cut(s) 253, 529, 764
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 5 cut(s) 28, 303, 355, 715, 797
TaqI TCGA 2 cut(s) 705, 787
TasI AATT 5 cut(s) 12, 249, 345, 550, 576
TatI WGTACW 1 cut(s) 44
TfiI GAWTC 1 cut(s) 568
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 3 cut(s) 81, 346, 373
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 1 cut(s) 519
Tsp45I GTSAC 1 cut(s) 385
TspRI CASTG 3 cut(s) 81, 346, 373
Van91I CCANNNNNTGG 1 cut(s) 16
XapI RAATTY 3 cut(s) 12, 249, 345
XceI RCATGY 3 cut(s) 45, 680, 844
XhoI CTCGAG 2 cut(s) 704, 786
XspI CTAG 5 cut(s) 32, 69, 461, 593, 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.