RLG00000032760

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0025438
Physical Location & Seq
Forward (+)
18731717 .. 18732611
895 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032760

Sequence Viewer

Length: 750 bp
ATGGCAGCTGCTAAATCCACAAGGCGTATATGGGTCCCGGAGCCAGTGATAGTGGGAGCAGGTCCTTCCGGCTTAGCTGCCGTGGCTTGTCTCAAGGAAAAAGGTATTCCGAGCTTAATCCTAGAAAGGACCAACTGCCTAGCTTTGTTGTGGCAGCTTAAGACCTACGACCGTCTCTGTCTTCATCTCCCAAAACAGTTCTGCGAGCTCATACTCATGCCATTCCCTGCAGATTTCCCAACCTACCCAACCAAGCATCAGTTCGTGAGTTACTTAAACGCTTATGCCGAGAGCTTTGATCTAAAGCCGGCTTTCAACACGACGGTGGTGACTGGTGAGTGCCAAACTTTGGGGTTGAAAAATGAGGAGGCGATTGAGTATGTTTGTCAGTGGCTGATCGTCGCCACCGGAGAAAATGTTGAGGAGGTTGTGCCGGAGTTTGAAGGAGTGAGGGACTTTGGTGCCCATTGTCCATACCAGCTCTTACAAGAGCGGCGAGCGCTTCGGGGGGAAGAAGGTCCTAGTGGTCGGTGTGGGAATTCAGTCATGGAGGTTTGCTTGGATCTCTGCGATTATAATGCAAAGCCTTCCCTTGTGGATAAAAATTCGCTCCAGATCAGAGCACTTATTCGTATTCATGACGAGTATATTAGATGTAAACTGACAATGGGCCCACCAAAGTCCCTCACTCCTCCAAACTCTGGCACAACCTCCTCAGCATTTTCTCCGGTGGCGACGATCAGCCACTGA

Protein Analysis

250

Amino Acids

27.55

Weight (kDa)

5.99

Isoelectric Point (pI)

47.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_3 PF13738 16 - 195 2.3e-11 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 17 - 111 3e-08 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0025438)

Species Orthologous Gene IDs
rosa_laevigata RLG00000032760
rosa_multiflora Rmu_sc0004797.1_g000005 Rmu_sc0004797.1_g000006

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 576
Acc36I ACCTGC 1 cut(s) 50
AccB1I GGYRCC 1 cut(s) 461
AccB7I CCANNNNNTGG 2 cut(s) 349, 701
AccBSI CCGCTC 1 cut(s) 493
AciI CCGC 1 cut(s) 493
AclWI GGATC 1 cut(s) 570
AcsI RAATTY 2 cut(s) 538, 604
AfeI AGCGCT 1 cut(s) 501
AfiI CCNNNNNNNGG 2 cut(s) 349, 701
AflII CTTAAG 1 cut(s) 158
AgsI TTSAA 3 cut(s) 316, 358, 443
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 8 cut(s) 8, 77, 114, 143, 157, 208, 294, 481
AluI AGCT 8 cut(s) 8, 77, 114, 143, 157, 208, 294, 481
Alw21I GWGCWC 2 cut(s) 210, 625
Alw26I GTCTC 2 cut(s) 95, 179
AlwI GGATC 1 cut(s) 570
AlwNI CAGNNNCTG 2 cut(s) 394, 747
Aor51HI AGCGCT 1 cut(s) 501
AoxI GGCC 1 cut(s) 670
ApaI GGGCCC 1 cut(s) 674
ApeKI GCWGC 4 cut(s) 5, 8, 77, 154
ApoI RAATTY 2 cut(s) 538, 604
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 6 cut(s) 34, 62, 129, 518, 670, 671
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 2 cut(s) 340, 347
AvaII GGWCC 4 cut(s) 34, 62, 129, 518
BaeGI GKGCMC 2 cut(s) 466, 674
BanI GGYRCC 1 cut(s) 461
BanII GRGCYC 2 cut(s) 210, 674
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 2 cut(s) 210, 625
BbvCI CCTCAGC 1 cut(s) 715
BbvI GCAGC 3 cut(s) 17, 64, 166
BceAI ACGGC 1 cut(s) 65
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 2 cut(s) 95, 179
BfaI CTAG 3 cut(s) 122, 140, 522
BfmI CTRYAG 1 cut(s) 228
BfoI RGCGCY 1 cut(s) 503
BfrI CTTAAG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 50
BisI GCNGC 5 cut(s) 6, 9, 78, 155, 494
BlpI GCTNAGC 1 cut(s) 73
BlsI GCNGC 5 cut(s) 7, 10, 79, 156, 495
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 4 cut(s) 34, 62, 129, 518
BmgT120I GGNCC 6 cut(s) 34, 62, 129, 518, 670, 671
BmiI GGNNCC 5 cut(s) 35, 36, 42, 463, 672
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 1 cut(s) 265
BpiI GAAGAC 1 cut(s) 173
BpmI CTGGAG 1 cut(s) 596
Bpu10I CCTNAGC 1 cut(s) 715
Bpu1102I GCTNAGC 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 81
BsaWI WCCGGW 2 cut(s) 407, 727
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 701
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 2 cut(s) 44, 337
BseDI CCNNGG 1 cut(s) 81
BseLI CCNNNNNNNGG 2 cut(s) 349, 701
BseMII CTCAG 1 cut(s) 729
BseNI ACTGG 2 cut(s) 44, 337
BseRI GAGGAG 4 cut(s) 380, 437, 681, 703
BseSI GKGCMC 2 cut(s) 466, 674
BseXI GCAGC 3 cut(s) 17, 64, 166
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 1 cut(s) 672
BshNI GGYRCC 1 cut(s) 461
BsiEI CGRYCG 1 cut(s) 172
BsiHKAI GWGCWC 2 cut(s) 210, 625
BsiSI CCGG 6 cut(s) 38, 69, 308, 408, 434, 728
BslFI GGGAC 3 cut(s) 20, 467, 667
BslI CCNNNNNNNGG 2 cut(s) 349, 701
BsmAI GTCTC 2 cut(s) 95, 179
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 3 cut(s) 20, 467, 667
BsnI GGCC 1 cut(s) 672
Bsp120I GGGCCC 1 cut(s) 670
Bsp1286I GDGCHC 4 cut(s) 210, 466, 625, 674
Bsp143I GATC 5 cut(s) 298, 396, 562, 615, 738
Bsp1720I GCTNAGC 1 cut(s) 73
BspACI CCGC 1 cut(s) 493
BspANI GGCC 1 cut(s) 672
BspCNI CTCAG 1 cut(s) 728
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 5 cut(s) 35, 36, 42, 463, 672
BspMAI CTGCAG 1 cut(s) 232
BspMI ACCTGC 1 cut(s) 50
BspPI GGATC 1 cut(s) 570
BspT107I GGYRCC 1 cut(s) 461
BspTI CTTAAG 1 cut(s) 158
BsrBI CCGCTC 1 cut(s) 493
BsrFI RCCGGY 1 cut(s) 307
BsrI ACTGG 2 cut(s) 44, 337
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 5 cut(s) 298, 396, 562, 615, 738
Bst4CI ACNGT 3 cut(s) 173, 198, 325
BstAFI CTTAAG 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 206, 309, 498
BstDEI CTNAG 2 cut(s) 73, 715
BstDSI CCRYGG 1 cut(s) 81
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 5 cut(s) 301, 399, 565, 618, 741
BstMAI GTCTC 2 cut(s) 95, 179
BstMBI GATC 5 cut(s) 298, 396, 562, 615, 738
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 2 cut(s) 83, 499
BstSCI CCNGG 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 228
BstSLI GKGCMC 2 cut(s) 466, 674
BstV1I GCAGC 3 cut(s) 17, 64, 166
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 1 cut(s) 562
BstYI RGATCY 1 cut(s) 562
BsuRI GGCC 1 cut(s) 672
BtgI CCRYGG 1 cut(s) 81
BtsIMutI CAGTG 3 cut(s) 51, 395, 745
BveI ACCTGC 1 cut(s) 50
Cac8I GCNNGC 3 cut(s) 206, 309, 498
CaiI CAGNNNCTG 2 cut(s) 394, 747
CciI TCATGA 1 cut(s) 637
CfoI GCGC 1 cut(s) 502
Cfr10I RCCGGY 1 cut(s) 307
Cfr13I GGNCC 6 cut(s) 34, 62, 129, 518, 670, 671
CviAII CATG 3 cut(s) 217, 547, 638
DdeI CTNAG 2 cut(s) 73, 715
DpnI GATC 5 cut(s) 300, 398, 564, 617, 740
DpnII GATC 5 cut(s) 298, 396, 562, 615, 738
Ecl136II GAGCTC 1 cut(s) 208
Eco24I GRGCYC 2 cut(s) 210, 674
Eco47I GGWCC 4 cut(s) 34, 62, 129, 518
Eco47III AGCGCT 1 cut(s) 501
Eco53kI GAGCTC 1 cut(s) 208
EcoICRI GAGCTC 1 cut(s) 208
EcoO109I RGGNCCY 3 cut(s) 34, 62, 518
EcoRI GAATTC 1 cut(s) 538
EcoT38I GRGCYC 2 cut(s) 210, 674
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 3 cut(s) 220, 550, 641
FaqI GGGAC 3 cut(s) 20, 467, 667
FatI CATG 3 cut(s) 216, 546, 637
Fnu4HI GCNGC 5 cut(s) 6, 9, 78, 155, 494
FriOI GRGCYC 2 cut(s) 210, 674
Fsp4HI GCNGC 5 cut(s) 6, 9, 78, 155, 494
FspBI CTAG 3 cut(s) 122, 140, 522
GlaI GCGC 1 cut(s) 501
GluI GCNGC 5 cut(s) 6, 9, 78, 155, 494
GsuI CTGGAG 1 cut(s) 596
HaeII RGCGCY 1 cut(s) 503
HaeIII GGCC 1 cut(s) 672
HapII CCGG 6 cut(s) 38, 69, 308, 408, 434, 728
HhaI GCGC 1 cut(s) 502
Hin1II CATG 3 cut(s) 220, 550, 641
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HpaII CCGG 6 cut(s) 38, 69, 308, 408, 434, 728
HphI GGTGA 2 cut(s) 340, 347
Hpy166II GTNNAC 1 cut(s) 659
Hpy188I TCNGA 2 cut(s) 111, 620
Hpy188III TCNNGA 3 cut(s) 265, 613, 638
Hpy8I GTNNAC 1 cut(s) 659
Hpy99I CGWCG 3 cut(s) 325, 404, 739
HpyAV CCTTC 4 cut(s) 75, 437, 509, 597
HpyCH4III ACNGT 3 cut(s) 173, 198, 325
HpyCH4V TGCA 2 cut(s) 230, 581
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 499
HpyF3I CTNAG 2 cut(s) 73, 715
Hsp92II CATG 3 cut(s) 220, 550, 641
HspAI GCGC 1 cut(s) 500
KflI GGGWCCC 1 cut(s) 34
KroI GCCGGC 1 cut(s) 307
KroNI GCCGGC 1 cut(s) 309
Kzo9I GATC 5 cut(s) 298, 396, 562, 615, 738
LmnI GCTCC 3 cut(s) 40, 56, 615
Lsp1109I GCAGC 3 cut(s) 17, 64, 166
LweI GCATC 1 cut(s) 265
MaeI CTAG 3 cut(s) 122, 140, 522
MaeIII GTNAC 2 cut(s) 269, 328
MalI GATC 5 cut(s) 300, 398, 564, 617, 740
MbiI CCGCTC 1 cut(s) 493
MboI GATC 5 cut(s) 298, 396, 562, 615, 738
MboII GAAGA 2 cut(s) 173, 524
MflI RGATCY 1 cut(s) 562
MhlI GDGCHC 4 cut(s) 210, 466, 625, 674
MluCI AATT 2 cut(s) 538, 604
MroNI GCCGGC 1 cut(s) 307
MseI TTAA 3 cut(s) 116, 159, 275
MslI CAYNNNNRTG 2 cut(s) 215, 323
MspA1I CMGCKG 1 cut(s) 8
MspCI CTTAAG 1 cut(s) 158
MspI CCGG 6 cut(s) 38, 69, 308, 408, 434, 728
MspR9I CCNGG 1 cut(s) 38
MwoI GCNNNNNNNGC 2 cut(s) 83, 499
NaeI GCCGGC 1 cut(s) 309
NciI CCSGG 1 cut(s) 38
NdeII GATC 5 cut(s) 298, 396, 562, 615, 738
NgoMIV GCCGGC 1 cut(s) 307
NlaIII CATG 3 cut(s) 220, 550, 641
NlaIV GGNNCC 5 cut(s) 35, 36, 42, 463, 672
NmeAIII GCCGAG 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 328
OliI CACNNNNGTG 1 cut(s) 323
PagI TCATGA 1 cut(s) 637
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PdiI GCCGGC 1 cut(s) 309
PflMI CCANNNNNTGG 2 cut(s) 349, 701
PfoI TCCNGGA 1 cut(s) 36
PkrI GCNGC 5 cut(s) 7, 10, 79, 156, 495
PpuMI RGGWCCY 3 cut(s) 34, 62, 518
PsiI TTATAA 1 cut(s) 576
Psp124BI GAGCTC 1 cut(s) 210
Psp5II RGGWCCY 3 cut(s) 34, 62, 518
PspN4I GGNNCC 5 cut(s) 35, 36, 42, 463, 672
PspOMI GGGCCC 1 cut(s) 670
PspPI GGNCC 6 cut(s) 34, 62, 129, 518, 670, 671
PspPPI RGGWCCY 3 cut(s) 34, 62, 518
PstI CTGCAG 1 cut(s) 232
PstNI CAGNNNCTG 2 cut(s) 394, 747
PsuI RGATCY 1 cut(s) 562
PvuII CAGCTG 1 cut(s) 8
RseI CAYNNNNRTG 2 cut(s) 215, 323
SacI GAGCTC 1 cut(s) 210
SaqAI TTAA 3 cut(s) 116, 159, 275
SatI GCNGC 5 cut(s) 6, 9, 78, 155, 494
Sau3AI GATC 5 cut(s) 298, 396, 562, 615, 738
Sau96I GGNCC 6 cut(s) 34, 62, 129, 518, 670, 671
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 4 cut(s) 210, 466, 625, 674
SfaNI GCATC 1 cut(s) 265
SfcI CTRYAG 1 cut(s) 228
SinI GGWCC 4 cut(s) 34, 62, 129, 518
SmiMI CAYNNNNRTG 2 cut(s) 215, 323
SmlI CTYRAG 2 cut(s) 92, 158
SmoI CTYRAG 2 cut(s) 92, 158
Sse9I AATT 2 cut(s) 538, 604
SsiI CCGC 1 cut(s) 493
SspMI CTAG 3 cut(s) 122, 140, 522
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 36
TaaI ACNGT 3 cut(s) 173, 198, 325
TasI AATT 2 cut(s) 538, 604
TauI GCSGC 1 cut(s) 496
Tru1I TTAA 3 cut(s) 116, 159, 275
Tru9I TTAA 3 cut(s) 116, 159, 275
TscAI CASTG 2 cut(s) 51, 395
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 4 cut(s) 5, 8, 77, 154
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 2 cut(s) 173, 626
TspRI CASTG 2 cut(s) 51, 395
Van91I CCANNNNNTGG 2 cut(s) 349, 701
Vha464I CTTAAG 1 cut(s) 158
VpaK11BI GGWCC 4 cut(s) 34, 62, 129, 518
XapI RAATTY 2 cut(s) 538, 604
XspI CTAG 3 cut(s) 122, 140, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.