RLG00000035282

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0030993
Physical Location & Seq
Forward (+)
64180207 .. 64182725
2519 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035282

Sequence Viewer

Length: 378 bp
ATGATCCGTACAAGCTGGCTCCATAAAAAGGGATTCGTATTGCTCTACTATTGCAGCTCTTCTTTGGTTGATGAGTCGTTTTCAGAGTACAGGCTTGGGTTTGGTTGCTCTGTGCTATACTCTGAAGGACCCTTAATTAGGACGTCAACAGTTCTGGGCCTGACAAAGGATGATCCACAGTTTAGAAATGCGCTTGAGAAGTGTGGAAAAGAAGCGGTGATGGTTAAAGGGGTATATGCAAATGTTGACAACGCCGCGGGAATAAGAGAGGGTCTGAGAGCAGGTTTTGTGGTAGCACTCCTTGCAATCTCACCAGCACATCGAGAAGTGAAGGAATTAGAACTGGCCCGTGCCAAAGTCATGCATCGAATTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

126

Amino Acids

13.87

Weight (kDa)

9.07

Isoelectric Point (pI)

27.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030993)

Species Orthologous Gene IDs
rosa_laevigata RLG00000035282
rosa_samantha Rh5AG386300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 272
AccII CGCG 1 cut(s) 257
AciI CCGC 3 cut(s) 215, 255, 257
AclWI GGATC 1 cut(s) 167
AcuI CTGAAG 1 cut(s) 144
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 2 cut(s) 10, 89
AfiI CCNNNNNNNGG 3 cut(s) 28, 138, 166
AluBI AGCT 2 cut(s) 15, 57
AluI AGCT 2 cut(s) 15, 57
AlwI GGATC 1 cut(s) 167
AoxI GGCC 2 cut(s) 157, 345
ApeKI GCWGC 1 cut(s) 54
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 3 cut(s) 128, 157, 346
AsuHPI GGTGA 2 cut(s) 229, 303
AvaII GGWCC 1 cut(s) 128
BbvI GCAGC 1 cut(s) 66
BccI CCATC 1 cut(s) 214
BfuAI ACCTGC 1 cut(s) 272
BisI GCNGC 2 cut(s) 55, 255
BlsI GCNGC 2 cut(s) 56, 256
Bme18I GGWCC 1 cut(s) 128
BmgT120I GGNCC 3 cut(s) 128, 157, 346
BmiI GGNNCC 2 cut(s) 20, 130
BmsI GCATC 1 cut(s) 373
BpuEI CTTGAG 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 255
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 138, 166
Bse1I ACTGG 1 cut(s) 348
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 3 cut(s) 28, 138, 166
BseMII CTCAG 1 cut(s) 266
BseNI ACTGG 1 cut(s) 348
BseXI GCAGC 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 257
BshFI GGCC 2 cut(s) 159, 347
BslI CCNNNNNNNGG 3 cut(s) 28, 138, 166
BsnI GGCC 2 cut(s) 159, 347
Bsp143I GATC 2 cut(s) 3, 172
BspACI CCGC 3 cut(s) 215, 255, 257
BspANI GGCC 2 cut(s) 159, 347
BspCNI CTCAG 1 cut(s) 267
BspFNI CGCG 1 cut(s) 257
BspLI GGNNCC 2 cut(s) 20, 130
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 1 cut(s) 167
BspQI GCTCTTC 1 cut(s) 64
BsrI ACTGG 1 cut(s) 348
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 2 cut(s) 3, 172
BssNI GRCGYC 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 151, 180
Bst6I CTCTTC 1 cut(s) 64
BstACI GRCGYC 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 17
BstDEI CTNAG 2 cut(s) 275, 375
BstDSI CCRYGG 1 cut(s) 255
BstENI CCTNNNNNAGG 2 cut(s) 136, 164
BstF5I GGATG 1 cut(s) 175
BstFNI CGCG 1 cut(s) 257
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 2 cut(s) 6, 175
BstMBI GATC 2 cut(s) 3, 172
BstMWI GCNNNNNNNGC 1 cut(s) 302
BstUI CGCG 1 cut(s) 257
BstV1I GCAGC 1 cut(s) 66
BsuRI GGCC 2 cut(s) 159, 347
BtgI CCRYGG 1 cut(s) 255
BtsCI GGATG 1 cut(s) 175
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 1 cut(s) 17
CfoI GCGC 1 cut(s) 193
Cfr13I GGNCC 3 cut(s) 128, 157, 346
Cfr42I CCGCGG 1 cut(s) 258
Csp6I GTAC 2 cut(s) 9, 88
CviAII CATG 1 cut(s) 361
CviJI RGCY 6 cut(s) 15, 19, 57, 94, 159, 347
CviKI_1 RGCY 6 cut(s) 15, 19, 57, 94, 159, 347
CviQI GTAC 2 cut(s) 9, 88
DdeI CTNAG 2 cut(s) 275, 375
DpnI GATC 2 cut(s) 5, 174
DpnII GATC 2 cut(s) 3, 172
Eam1104I CTCTTC 1 cut(s) 64
EarI CTCTTC 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 144
EcoNI CCTNNNNNAGG 2 cut(s) 136, 164
EcoO109I RGGNCCY 1 cut(s) 128
EcoT22I ATGCAT 1 cut(s) 366
FaeI CATG 1 cut(s) 364
FaiI YATR 5 cut(s) 24, 118, 235, 237, 362
FatI CATG 1 cut(s) 360
FauI CCCGC 1 cut(s) 250
Fnu4HI GCNGC 2 cut(s) 55, 255
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 2 cut(s) 55, 255
GlaI GCGC 1 cut(s) 192
GluI GCNGC 2 cut(s) 55, 255
HaeIII GGCC 2 cut(s) 159, 347
HhaI GCGC 1 cut(s) 193
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 1 cut(s) 364
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HincII GTYRAC 2 cut(s) 147, 247
HindII GTYRAC 2 cut(s) 147, 247
HinfI GANTC 2 cut(s) 33, 74
HphI GGTGA 2 cut(s) 229, 303
Hpy166II GTNNAC 2 cut(s) 147, 247
Hpy188I TCNGA 3 cut(s) 85, 124, 276
Hpy188III TCNNGA 1 cut(s) 323
Hpy8I GTNNAC 2 cut(s) 147, 247
HpyAV CCTTC 2 cut(s) 119, 325
HpyCH4III ACNGT 2 cut(s) 151, 180
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 4 cut(s) 54, 239, 305, 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 302
HpyF3I CTNAG 2 cut(s) 275, 375
HpySE526I ACGT 1 cut(s) 143
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 1 cut(s) 364
HspAI GCGC 1 cut(s) 191
KspI CCGCGG 1 cut(s) 258
Kzo9I GATC 2 cut(s) 3, 172
LguI GCTCTTC 1 cut(s) 64
LmnI GCTCC 1 cut(s) 24
LpnPI CCDG 6 cut(s) 76, 140, 173, 267, 327, 329
Lsp1109I GCAGC 1 cut(s) 66
LweI GCATC 1 cut(s) 373
MaeII ACGT 1 cut(s) 143
MalI GATC 2 cut(s) 5, 174
MboI GATC 2 cut(s) 3, 172
MboII GAAGA 1 cut(s) 51
MluCI AATT 3 cut(s) 135, 335, 369
MlyI GAGTC 1 cut(s) 83
MnlI CCTC 1 cut(s) 262
Mph1103I ATGCAT 1 cut(s) 366
MseI TTAA 2 cut(s) 134, 225
MspA1I CMGCKG 1 cut(s) 257
MvnI CGCG 1 cut(s) 257
MwoI GCNNNNNNNGC 1 cut(s) 302
NdeII GATC 2 cut(s) 3, 172
NlaIII CATG 1 cut(s) 364
NlaIV GGNNCC 2 cut(s) 20, 130
NsiI ATGCAT 1 cut(s) 366
PciSI GCTCTTC 1 cut(s) 64
PfeI GAWTC 1 cut(s) 33
PkrI GCNGC 2 cut(s) 56, 256
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
PpuMI RGGWCCY 1 cut(s) 128
Psp5II RGGWCCY 1 cut(s) 128
PspN4I GGNNCC 2 cut(s) 20, 130
PspPI GGNCC 3 cut(s) 128, 157, 346
PspPPI RGGWCCY 1 cut(s) 128
RsaI GTAC 2 cut(s) 10, 89
RsaNI GTAC 2 cut(s) 9, 88
SacII CCGCGG 1 cut(s) 258
SapI GCTCTTC 1 cut(s) 64
SaqAI TTAA 2 cut(s) 134, 225
SatI GCNGC 2 cut(s) 55, 255
Sau3AI GATC 2 cut(s) 3, 172
Sau96I GGNCC 3 cut(s) 128, 157, 346
SchI GAGTC 1 cut(s) 83
SetI ASST 4 cut(s) 17, 59, 146, 286
SfaNI GCATC 1 cut(s) 373
Sfr303I CCGCGG 1 cut(s) 258
SgrBI CCGCGG 1 cut(s) 258
SinI GGWCC 1 cut(s) 128
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 3 cut(s) 135, 335, 369
SsiI CCGC 3 cut(s) 215, 255, 257
TaaI ACNGT 2 cut(s) 151, 180
TaiI ACGT 1 cut(s) 146
TaqI TCGA 2 cut(s) 322, 367
TasI AATT 3 cut(s) 135, 335, 369
TatI WGTACW 1 cut(s) 87
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 1 cut(s) 33
Tru1I TTAA 2 cut(s) 134, 225
Tru9I TTAA 2 cut(s) 134, 225
TseI GCWGC 1 cut(s) 54
VpaK11BI GGWCC 1 cut(s) 128
XagI CCTNNNNNAGG 2 cut(s) 136, 164
ZraI GACGTC 1 cut(s) 144
Zsp2I ATGCAT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.