RLG00000035564

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0030148
Physical Location & Seq
Reverse (-)
68613558 .. 68614795
1238 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035564

Sequence Viewer

Length: 786 bp
ATGACATACAATGCTTTCTTTGCATTTGCACCTTATGCACATGACCGGAAGTCCACCAGGAAGTTGACCATGAATGAGCTCCTAGGCGGCCGTAGCAAGTACGTGAGGCTTCTGAGGCTGTTAGCCAAGAAAGCTGAGACTTGCGAATCAGTCAACCTCAGTGAGGATTGTCGAGGTGCTTCGTTGGCGGAAGAGACGAAGTTGGTGGTCTGGGAGGCGGCCAAGATTTTCAGCGAGTTCAATTTCAGTGAAACTAATAAGACCATACATAAGAAGTTCGATGAATTGGTGGAGAAGGTGATCGCTGAACGTGAAGAGTTGAGGAAGATGCAGAAGAAAGGCAACACTGGAGAAGAAAAGGATGTCAAGAGCTTTCTTGATATTTTGCTTGATATTTTGGAGGGTGAGAGTAGTGAGAATTTGGAGGTTGAATTTTCAAGAAATCATGTTAAGGGTCTAATCACGGATTTGTTCGCGGCTAGCACAGACACAACCTCCATTTCAATGGAATGGGCGCTGGCAGAGCTTATCAATCACCCAGGGATGCTTACAAAAGAAAGGGATGAGATAGATCGAGTCGTTGAAAATGGACGATTAGTCGTCGGAGAATTGGATGTTCCCAATCTTTCCTCCATCCAAGCCATCATAAAAGAATCATTTAGGCTACACCCACCATTGACTTTAGTCGCAAGAAAATGTGTAGAACAATATAAGGTTGGCAAACATGTCATTGCCAAGACATTTGGGCCATCGGAAGAGATCCTAAGAATTGGGAAAAGCCATTAG

Protein Analysis

262

Amino Acids

29.6

Weight (kDa)

6.22

Isoelectric Point (pI)

29.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 81 - 246 1.4e-29 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030148)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0062531
rosa_laevigata RLG00000035564

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 476
AciI CCGC 4 cut(s) 87, 188, 218, 476
AclWI GGATC 1 cut(s) 754
AcoI YGGCCR 2 cut(s) 88, 219
AcsI RAATTY 2 cut(s) 418, 431
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 163
AflIII ACRYGT 1 cut(s) 724
AgsI TTSAA 5 cut(s) 241, 431, 438, 504, 584
AhdI GACNNNNNGTC 2 cut(s) 49, 596
AjnI CCWGG 2 cut(s) 56, 538
AluBI AGCT 4 cut(s) 79, 134, 372, 526
AluI AGCT 4 cut(s) 79, 134, 372, 526
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 2 cut(s) 131, 188
AlwI GGATC 1 cut(s) 754
AoxI GGCC 3 cut(s) 88, 219, 746
ApoI RAATTY 2 cut(s) 418, 431
AspA2I CCTAGG 1 cut(s) 82
AspLEI GCGC 1 cut(s) 517
AspS9I GGNCC 1 cut(s) 746
AsuHPI GGTGA 3 cut(s) 310, 416, 527
AsuNHI GCTAGC 1 cut(s) 479
AvrII CCTAGG 1 cut(s) 82
BanII GRGCYC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 81
BccI CCATC 3 cut(s) 641, 650, 757
BceAI ACGGC 1 cut(s) 75
BciT130I CCWGG 2 cut(s) 58, 540
BcoDI GTCTC 2 cut(s) 131, 188
BfaI CTAG 2 cut(s) 83, 480
BfoI RGCGCY 1 cut(s) 518
BisI GCNGC 3 cut(s) 88, 219, 477
BlnI CCTAGG 1 cut(s) 82
BlsI GCNGC 3 cut(s) 89, 220, 478
Bme1390I CCNGG 2 cut(s) 58, 540
BmeRI GACNNNNNGTC 2 cut(s) 49, 596
BmgT120I GGNCC 1 cut(s) 746
BmrFI CCNGG 2 cut(s) 58, 540
BmsI GCATC 2 cut(s) 318, 534
BmtI GCTAGC 1 cut(s) 483
BoxI GACNNNNGTC 1 cut(s) 683
BpmI CTGGAG 1 cut(s) 369
BsaAI YACGTR 1 cut(s) 103
BsaJI CCNNGG 3 cut(s) 82, 538, 539
BsaWI WCCGGW 1 cut(s) 45
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bsc4I CCNNNNNNNGG 1 cut(s) 163
Bse1I ACTGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 729
BseBI CCWGG 2 cut(s) 58, 540
BseDI CCNNGG 3 cut(s) 82, 538, 539
BseGI GGATG 5 cut(s) 367, 549, 568, 619, 633
BseLI CCNNNNNNNGG 1 cut(s) 163
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 3 cut(s) 104, 126, 172
BseNI ACTGG 1 cut(s) 352
BseX3I CGGCCG 1 cut(s) 88
Bsh1236I CGCG 1 cut(s) 476
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 3 cut(s) 90, 221, 748
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 1 cut(s) 46
BslI CCNNNNNNNGG 1 cut(s) 163
BsmAI GTCTC 2 cut(s) 131, 188
BsmBI CGTCTC 1 cut(s) 188
BsnI GGCC 3 cut(s) 90, 221, 748
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 3 cut(s) 300, 571, 759
BspACI CCGC 4 cut(s) 87, 188, 218, 476
BspANI GGCC 3 cut(s) 90, 221, 748
BspCNI CTCAG 3 cut(s) 105, 127, 171
BspFNI CGCG 1 cut(s) 476
BspOI GCTAGC 1 cut(s) 483
BspPI GGATC 1 cut(s) 754
BsrDI GCAATG 1 cut(s) 729
BsrI ACTGG 1 cut(s) 352
BssECI CCNNGG 3 cut(s) 82, 538, 539
BssMI GATC 3 cut(s) 300, 571, 759
BssT1I CCWWGG 1 cut(s) 82
Bst2UI CCWGG 2 cut(s) 58, 540
Bst6I CTCTTC 3 cut(s) 186, 309, 750
BstAPI GCANNNNNTGC 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 103
BstC8I GCNNGC 2 cut(s) 481, 519
BstDEI CTNAG 4 cut(s) 113, 135, 158, 764
BstENI CCTNNNNNAGG 1 cut(s) 161
BstF5I GGATG 5 cut(s) 367, 549, 568, 619, 633
BstFNI CGCG 1 cut(s) 476
BstH2I RGCGCY 1 cut(s) 518
BstHHI GCGC 1 cut(s) 517
BstKTI GATC 3 cut(s) 303, 574, 762
BstMAI GTCTC 2 cut(s) 131, 188
BstMBI GATC 3 cut(s) 300, 571, 759
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 7 cut(s) 20, 35, 93, 115, 131, 185, 523
BstNI CCWGG 2 cut(s) 58, 540
BstNSI RCATGY 1 cut(s) 728
BstPAI GACNNNNGTC 1 cut(s) 683
BstSCI CCNGG 2 cut(s) 56, 538
BstUI CGCG 1 cut(s) 476
BstX2I RGATCY 1 cut(s) 759
BstXI CCANNNNNNTGG 1 cut(s) 505
BstYI RGATCY 1 cut(s) 759
BstZI CGGCCG 1 cut(s) 88
BsuRI GGCC 3 cut(s) 90, 221, 748
BtsCI GGATG 5 cut(s) 367, 549, 568, 619, 633
BtsIMutI CAGTG 3 cut(s) 166, 253, 345
Cac8I GCNNGC 2 cut(s) 481, 519
CfoI GCGC 1 cut(s) 517
Cfr13I GGNCC 1 cut(s) 746
Csp6I GTAC 1 cut(s) 100
CviAII CATG 4 cut(s) 41, 70, 446, 725
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 4 cut(s) 113, 135, 158, 764
DpnI GATC 3 cut(s) 302, 573, 761
DpnII GATC 3 cut(s) 300, 571, 759
DriI GACNNNNNGTC 2 cut(s) 49, 596
EaeI YGGCCR 2 cut(s) 88, 219
EagI CGGCCG 1 cut(s) 88
Eam1104I CTCTTC 3 cut(s) 186, 309, 750
Eam1105I GACNNNNNGTC 2 cut(s) 49, 596
EarI CTCTTC 3 cut(s) 186, 309, 750
EciI GGCGGA 1 cut(s) 203
Ecl136II GAGCTC 1 cut(s) 79
EclXI CGGCCG 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 81
Eco52I CGGCCG 1 cut(s) 88
Eco53kI GAGCTC 1 cut(s) 79
EcoICRI GAGCTC 1 cut(s) 79
EcoNI CCTNNNNNAGG 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 56, 538
EcoT14I CCWWGG 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 82
Esp3I CGTCTC 1 cut(s) 188
FaeI CATG 4 cut(s) 44, 73, 449, 728
FatI CATG 4 cut(s) 40, 69, 445, 724
Fnu4HI GCNGC 3 cut(s) 88, 219, 477
FokI GGATG 5 cut(s) 374, 556, 575, 620, 626
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 3 cut(s) 88, 219, 477
FspBI CTAG 2 cut(s) 83, 480
GlaI GCGC 1 cut(s) 516
GluI GCNGC 3 cut(s) 88, 219, 477
GsuI CTGGAG 1 cut(s) 369
HaeII RGCGCY 1 cut(s) 518
HaeIII GGCC 3 cut(s) 90, 221, 748
HapII CCGG 1 cut(s) 46
HhaI GCGC 1 cut(s) 517
Hin1II CATG 4 cut(s) 44, 73, 449, 728
Hin6I GCGC 1 cut(s) 515
HinP1I GCGC 1 cut(s) 515
HincII GTYRAC 2 cut(s) 66, 154
HindII GTYRAC 2 cut(s) 66, 154
HinfI GANTC 3 cut(s) 146, 576, 653
HpaII CCGG 1 cut(s) 46
HphI GGTGA 3 cut(s) 310, 416, 527
Hpy166II GTNNAC 3 cut(s) 54, 66, 154
Hpy188I TCNGA 3 cut(s) 114, 605, 754
Hpy188III TCNNGA 3 cut(s) 367, 377, 438
Hpy8I GTNNAC 3 cut(s) 54, 66, 154
Hpy99I CGWCG 1 cut(s) 605
HpyAV CCTTC 1 cut(s) 289
HpyCH4IV ACGT 2 cut(s) 102, 310
HpyCH4V TGCA 4 cut(s) 23, 29, 38, 331
HpyF10VI GCNNNNNNNGC 7 cut(s) 20, 35, 93, 115, 131, 185, 523
HpyF3I CTNAG 4 cut(s) 113, 135, 158, 764
HpySE526I ACGT 2 cut(s) 102, 310
Hsp92II CATG 4 cut(s) 44, 73, 449, 728
HspAI GCGC 1 cut(s) 515
Kzo9I GATC 3 cut(s) 300, 571, 759
LmnI GCTCC 1 cut(s) 84
LpnPI CCDG 8 cut(s) 43, 59, 70, 196, 333, 503, 525, 552
LweI GCATC 2 cut(s) 318, 534
MaeI CTAG 2 cut(s) 83, 480
MaeII ACGT 2 cut(s) 102, 310
MalI GATC 3 cut(s) 302, 573, 761
MboI GATC 3 cut(s) 300, 571, 759
MboII GAAGA 6 cut(s) 203, 326, 337, 346, 365, 767
MflI RGATCY 1 cut(s) 759
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 6 cut(s) 241, 284, 418, 431, 608, 768
MlyI GAGTC 1 cut(s) 585
MmeI TCCRAC 1 cut(s) 583
MseI TTAA 1 cut(s) 450
MslI CAYNNNNRTG 1 cut(s) 503
MspI CCGG 1 cut(s) 46
MspR9I CCNGG 2 cut(s) 58, 540
MvaI CCWGG 2 cut(s) 58, 540
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 7 cut(s) 20, 35, 93, 115, 131, 185, 523
NdeII GATC 3 cut(s) 300, 571, 759
NheI GCTAGC 1 cut(s) 479
NlaIII CATG 4 cut(s) 44, 73, 449, 728
NspI RCATGY 1 cut(s) 728
PasI CCCWGGG 1 cut(s) 539
PciI ACATGT 1 cut(s) 724
PfeI GAWTC 2 cut(s) 146, 653
PkrI GCNGC 3 cut(s) 89, 220, 478
PleI GAGTC 1 cut(s) 584
PpsI GAGTC 1 cut(s) 584
Ppu21I YACGTR 1 cut(s) 103
PscI ACATGT 1 cut(s) 724
PshAI GACNNNNGTC 1 cut(s) 683
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 2 cut(s) 56, 538
PspGI CCWGG 2 cut(s) 56, 538
PspPI GGNCC 1 cut(s) 746
PsuI RGATCY 1 cut(s) 759
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 503
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 3 cut(s) 88, 219, 477
Sau3AI GATC 3 cut(s) 300, 571, 759
Sau96I GGNCC 1 cut(s) 746
SchI GAGTC 1 cut(s) 585
ScrFI CCNGG 2 cut(s) 58, 540
SduI GDGCHC 1 cut(s) 81
SfaNI GCATC 2 cut(s) 318, 534
SmiMI CAYNNNNRTG 1 cut(s) 503
Sse9I AATT 6 cut(s) 241, 284, 418, 431, 608, 768
SsiI CCGC 4 cut(s) 87, 188, 218, 476
SspMI CTAG 2 cut(s) 83, 480
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 2 cut(s) 56, 538
StyI CCWWGG 1 cut(s) 82
TaiI ACGT 2 cut(s) 105, 313
TaqI TCGA 3 cut(s) 172, 279, 574
TasI AATT 6 cut(s) 241, 284, 418, 431, 608, 768
TauI GCSGC 3 cut(s) 90, 221, 479
TfiI GAWTC 2 cut(s) 146, 653
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TscAI CASTG 3 cut(s) 166, 253, 352
TspDTI ATGAA 2 cut(s) 86, 297
TspGWI ACGGA 1 cut(s) 479
TspRI CASTG 3 cut(s) 166, 253, 352
XagI CCTNNNNNAGG 1 cut(s) 161
XapI RAATTY 2 cut(s) 418, 431
XceI RCATGY 1 cut(s) 728
XmaJI CCTAGG 1 cut(s) 82
XspI CTAG 2 cut(s) 83, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.