RLG00000035704

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0030912
Physical Location & Seq
Forward (+)
70548250 .. 70548636
387 bp
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UTR
Exon/CDS
Intron
RLM00000035704

Sequence Viewer

Length: 387 bp
ATGGCCTTGAGCACCCAAATAGCCTCTGCATCTTGTTCTTCATCAGCTGAATCATTTGCTTCCATGAGGAAGTACGATGTTTTTCTGAGTTTCAGGGGTGTAGATATTCGCAAGACTATTGTCCCCTCTTTATACCATGAATTGCAGAACAGAGAAATTAAAACGTTCATGGATGATACAGGGCTCGAAACTGGGAAGCCTATTTCTCCTAGTCTGCTAAGAGCAATCAAAGAATCCTGGTCAGCGATCGTTGTCCTGTCACCAAACTATGCTTCTTCACCATGGTGTTTGCATGAACTGACAATGATTGTTGAATGCATGGAAGACAAGAACTTGATAGTTCTCCCTAAGTCCCTTTTTTTTACGGTGTGGATCCCTCTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

129

Amino Acids

14.41

Weight (kDa)

6.08

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 116 1.1e-30 TIR domain
TIR_2 PF13676 27 - 115 5e-14 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030912)

Species Orthologous Gene IDs
rosa_laevigata RLG00000035704 RLG00000035710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 164
AclWI GGATC 2 cut(s) 367, 380
AfaI GTAC 1 cut(s) 74
AgsI TTSAA 1 cut(s) 314
AjnI CCWGG 1 cut(s) 236
AjuI GAANNNNNNNTTGG 2 cut(s) 256, 288
AleI CACNNNNGTG 1 cut(s) 283
AluBI AGCT 1 cut(s) 47
AluI AGCT 1 cut(s) 47
Alw21I GWGCWC 1 cut(s) 14
AlwI GGATC 2 cut(s) 367, 380
AoxI GGCC 1 cut(s) 3
AsuHPI GGTGA 2 cut(s) 252, 270
BamHI GGATCC 1 cut(s) 372
BanII GRGCYC 1 cut(s) 186
BbsI GAAGAC 1 cut(s) 330
Bbv12I GWGCWC 1 cut(s) 14
BciT130I CCWGG 1 cut(s) 238
BfaI CTAG 1 cut(s) 210
Bme1390I CCNGG 1 cut(s) 238
BmiI GGNNCC 1 cut(s) 374
BmrFI CCNGG 1 cut(s) 238
BmrI ACTGGG 1 cut(s) 201
BmsI GCATC 1 cut(s) 38
BmuI ACTGGG 1 cut(s) 201
BoxI GACNNNNGTC 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 28
BsaJI CCNNGG 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 196
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 1 cut(s) 281
BseGI GGATG 1 cut(s) 178
BseMII CTCAG 1 cut(s) 77
BseNI ACTGG 1 cut(s) 196
Bsh1285I CGRYCG 1 cut(s) 249
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 249
BsiHKAI GWGCWC 1 cut(s) 14
BslFI GGGAC 2 cut(s) 107, 337
BsmFI GGGAC 2 cut(s) 107, 337
BsmI GAATGC 1 cut(s) 320
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 14, 186
Bsp143I GATC 2 cut(s) 246, 372
Bsp19I CCATGG 1 cut(s) 281
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 78
BspLI GGNNCC 1 cut(s) 374
BspPI GGATC 2 cut(s) 367, 380
BsrI ACTGG 1 cut(s) 196
BssECI CCNNGG 1 cut(s) 281
BssMI GATC 2 cut(s) 246, 372
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 1 cut(s) 367
BstDEI CTNAG 3 cut(s) 86, 218, 348
BstDSI CCRYGG 1 cut(s) 281
BstF5I GGATG 1 cut(s) 178
BstKTI GATC 2 cut(s) 249, 375
BstMBI GATC 2 cut(s) 246, 372
BstMCI CGRYCG 1 cut(s) 249
BstNI CCWGG 1 cut(s) 238
BstPAI GACNNNNGTC 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 236
BstV2I GAAGAC 1 cut(s) 330
BstX2I RGATCY 1 cut(s) 372
BstYI RGATCY 1 cut(s) 372
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 281
BtsCI GGATG 1 cut(s) 178
Csp6I GTAC 1 cut(s) 73
CviAII CATG 6 cut(s) 64, 137, 169, 282, 293, 319
CviJI RGCY 5 cut(s) 5, 23, 47, 184, 199
CviKI_1 RGCY 5 cut(s) 5, 23, 47, 184, 199
CviQI GTAC 1 cut(s) 73
DdeI CTNAG 3 cut(s) 86, 218, 348
DpnI GATC 2 cut(s) 248, 374
DpnII GATC 2 cut(s) 246, 372
Eco130I CCWWGG 1 cut(s) 281
Eco24I GRGCYC 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 281
EcoT22I ATGCAT 1 cut(s) 320
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 281
FaeI CATG 6 cut(s) 67, 140, 172, 285, 296, 322
FaiI YATR 8 cut(s) 65, 133, 138, 170, 270, 283, 294, 320
FaqI GGGAC 2 cut(s) 107, 337
FatI CATG 6 cut(s) 63, 136, 168, 281, 292, 318
FokI GGATG 1 cut(s) 185
FriOI GRGCYC 1 cut(s) 186
FspBI CTAG 1 cut(s) 210
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 6 cut(s) 67, 140, 172, 285, 296, 322
HinfI GANTC 2 cut(s) 50, 233
HphI GGTGA 2 cut(s) 252, 270
Hpy188I TCNGA 2 cut(s) 87, 381
HpyCH4III ACNGT 1 cut(s) 367
HpyCH4IV ACGT 1 cut(s) 164
HpyCH4V TGCA 4 cut(s) 29, 145, 292, 318
HpyF3I CTNAG 3 cut(s) 86, 218, 348
HpySE526I ACGT 1 cut(s) 164
Hsp92II CATG 6 cut(s) 67, 140, 172, 285, 296, 322
Kzo9I GATC 2 cut(s) 246, 372
LpnPI CCDG 6 cut(s) 79, 165, 177, 223, 250, 269
LweI GCATC 1 cut(s) 38
MaeI CTAG 1 cut(s) 210
MaeII ACGT 1 cut(s) 164
MaeIII GTNAC 1 cut(s) 258
MalI GATC 2 cut(s) 248, 374
MboI GATC 2 cut(s) 246, 372
MboII GAAGA 3 cut(s) 30, 267, 335
MflI RGATCY 1 cut(s) 372
MhlI GDGCHC 2 cut(s) 14, 186
MluCI AATT 2 cut(s) 140, 156
MnlI CCTC 4 cut(s) 34, 60, 136, 387
Mph1103I ATGCAT 1 cut(s) 320
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 283
MspA1I CMGCKG 1 cut(s) 47
MspR9I CCNGG 1 cut(s) 238
Mva1269I GAATGC 1 cut(s) 320
MvaI CCWGG 1 cut(s) 238
NcoI CCATGG 1 cut(s) 281
NdeII GATC 2 cut(s) 246, 372
NlaIII CATG 6 cut(s) 67, 140, 172, 285, 296, 322
NlaIV GGNNCC 1 cut(s) 374
NmuCI GTSAC 1 cut(s) 258
NsiI ATGCAT 1 cut(s) 320
OliI CACNNNNGTG 1 cut(s) 283
PctI GAATGC 1 cut(s) 320
PfeI GAWTC 2 cut(s) 50, 233
Ple19I CGATCG 1 cut(s) 249
PshAI GACNNNNGTC 1 cut(s) 119
Psp1406I AACGTT 1 cut(s) 164
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 1 cut(s) 374
PsuI RGATCY 1 cut(s) 372
PvuI CGATCG 1 cut(s) 249
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
RseI CAYNNNNRTG 1 cut(s) 283
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 2 cut(s) 246, 372
ScrFI CCNGG 1 cut(s) 238
SduI GDGCHC 2 cut(s) 14, 186
SetI ASST 2 cut(s) 49, 167
SfaNI GCATC 1 cut(s) 38
SmiMI CAYNNNNRTG 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
Sse9I AATT 2 cut(s) 140, 156
SspMI CTAG 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 236
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 1 cut(s) 367
TaiI ACGT 1 cut(s) 167
TaqI TCGA 1 cut(s) 186
TasI AATT 2 cut(s) 140, 156
TfiI GAWTC 2 cut(s) 50, 233
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TseFI GTSAC 1 cut(s) 258
Tsp45I GTSAC 1 cut(s) 258
TspDTI ATGAA 4 cut(s) 30, 153, 157, 309
XspI CTAG 1 cut(s) 210
Zsp2I ATGCAT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.