RLG00000035710

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0030912
Physical Location & Seq
Forward (+)
70662936 .. 70663343
408 bp
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UTR
Exon/CDS
Intron
RLM00000035710

Sequence Viewer

Length: 408 bp
ATGGCCTTGAGCACCGAAAGACCATCTGGATCTCGTCCTTCATCATCTGCTCCTCCGGGGATGTATGATGTTTTTCTGAGTTTCAGGGGTGTAGACACTCGCAGGACTATTGCCTCCTCTATCTATCATGAACTGCAAAAAAGAGACATTAAAACGTTCATGGATGATACAGGGCTTAAAACAGGGAAGCCGATTTCTCCTAGTCTGCGAATAGCAATCAAAGAATCCTGGTCAGCGATCGTTGTCCTGTCACCAAACTATGCTTCTTCATCATGGTGTTTGCATGAGCTGGCGATGATTATTGAATGCATGGAAGACAAGAACATGATAGTTCTCCCAATTTTTTACGGTGTGGATCCCTCTGATGTGAGATATATAGAAAACGAGTTTTGGAGAAGCACTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

136

Amino Acids

15.26

Weight (kDa)

5.68

Isoelectric Point (pI)

67.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 126 5.1e-37 TIR domain
TIR_2 PF13676 24 - 124 1.1e-15 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030912)

Species Orthologous Gene IDs
rosa_laevigata RLG00000035704 RLG00000035710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 93
AclI AACGTT 1 cut(s) 155
AclWI GGATC 3 cut(s) 37, 350, 363
AgsI TTSAA 1 cut(s) 305
AjnI CCWGG 1 cut(s) 227
AjuI GAANNNNNNNTTGG 2 cut(s) 247, 279
AluBI AGCT 1 cut(s) 289
AluI AGCT 1 cut(s) 289
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 1 cut(s) 138
AlwI GGATC 3 cut(s) 37, 350, 363
AoxI GGCC 1 cut(s) 3
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 243
BamHI GGATCC 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 14
BccI CCATC 1 cut(s) 31
BciT130I CCWGG 1 cut(s) 229
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 2 cut(s) 201, 406
Bme1390I CCNGG 2 cut(s) 57, 229
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 2 cut(s) 57, 229
BpiI GAAGAC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 56
BseBI CCWGG 1 cut(s) 229
BseDI CCNNGG 1 cut(s) 56
BseGI GGATG 2 cut(s) 66, 169
BseMII CTCAG 1 cut(s) 68
BseRI GAGGAG 2 cut(s) 42, 106
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 14
BsiSI CCGG 1 cut(s) 56
BsmAI GTCTC 1 cut(s) 138
BsmI GAATGC 1 cut(s) 311
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 14
Bsp143I GATC 3 cut(s) 29, 237, 355
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 69
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 1 cut(s) 357
BspPI GGATC 3 cut(s) 37, 350, 363
BssECI CCNNGG 1 cut(s) 56
BssMI GATC 3 cut(s) 29, 237, 355
Bst2UI CCWGG 1 cut(s) 229
Bst4CI ACNGT 1 cut(s) 350
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 1 cut(s) 77
BstF5I GGATG 2 cut(s) 66, 169
BstKTI GATC 3 cut(s) 32, 240, 358
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 3 cut(s) 29, 237, 355
BstMCI CGRYCG 1 cut(s) 240
BstNI CCWGG 1 cut(s) 229
BstSCI CCNGG 2 cut(s) 55, 227
BstV2I GAAGAC 1 cut(s) 321
BstX2I RGATCY 2 cut(s) 29, 355
BstYI RGATCY 2 cut(s) 29, 355
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 308
BtsCI GGATG 2 cut(s) 66, 169
BtsIMutI CAGTG 1 cut(s) 399
Cac8I GCNNGC 1 cut(s) 291
CciI TCATGA 1 cut(s) 127
CviAII CATG 6 cut(s) 128, 160, 273, 284, 310, 325
CviJI RGCY 4 cut(s) 5, 175, 190, 289
CviKI_1 RGCY 4 cut(s) 5, 175, 190, 289
DdeI CTNAG 1 cut(s) 77
DpnI GATC 3 cut(s) 31, 239, 357
DpnII GATC 3 cut(s) 29, 237, 355
EcoRII CCWGG 1 cut(s) 227
EcoT22I ATGCAT 1 cut(s) 311
FaeI CATG 6 cut(s) 131, 163, 276, 287, 313, 328
FatI CATG 6 cut(s) 127, 159, 272, 283, 309, 324
FblI GTMKAC 1 cut(s) 93
FokI GGATG 2 cut(s) 73, 176
FspBI CTAG 2 cut(s) 201, 406
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 56
Hin1II CATG 6 cut(s) 131, 163, 276, 287, 313, 328
HinfI GANTC 1 cut(s) 224
HpaII CCGG 1 cut(s) 56
HphI GGTGA 1 cut(s) 243
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 2 cut(s) 78, 364
Hpy188III TCNNGA 2 cut(s) 27, 128
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 1 cut(s) 48
HpyCH4III ACNGT 1 cut(s) 350
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 3 cut(s) 136, 283, 309
HpyF3I CTNAG 1 cut(s) 77
HpySE526I ACGT 1 cut(s) 155
Hsp92II CATG 6 cut(s) 131, 163, 276, 287, 313, 328
Kzo9I GATC 3 cut(s) 29, 237, 355
LmnI GCTCC 1 cut(s) 55
MaeI CTAG 2 cut(s) 201, 406
MaeII ACGT 1 cut(s) 155
MaeIII GTNAC 1 cut(s) 249
MalI GATC 3 cut(s) 31, 239, 357
MboI GATC 3 cut(s) 29, 237, 355
MboII GAAGA 2 cut(s) 258, 326
MflI RGATCY 2 cut(s) 29, 355
MhlI GDGCHC 1 cut(s) 14
MluCI AATT 1 cut(s) 339
MnlI CCTC 4 cut(s) 63, 124, 127, 370
Mph1103I ATGCAT 1 cut(s) 311
MseI TTAA 2 cut(s) 150, 177
MslI CAYNNNNRTG 1 cut(s) 274
MspI CCGG 1 cut(s) 56
MspR9I CCNGG 2 cut(s) 57, 229
Mva1269I GAATGC 1 cut(s) 311
MvaI CCWGG 1 cut(s) 229
NciI CCSGG 1 cut(s) 57
NdeII GATC 3 cut(s) 29, 237, 355
NlaIII CATG 6 cut(s) 131, 163, 276, 287, 313, 328
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 1 cut(s) 249
NsiI ATGCAT 1 cut(s) 311
PagI TCATGA 1 cut(s) 127
PctI GAATGC 1 cut(s) 311
PfeI GAWTC 1 cut(s) 224
Ple19I CGATCG 1 cut(s) 240
Psp1406I AACGTT 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 227
PspGI CCWGG 1 cut(s) 227
PspN4I GGNNCC 1 cut(s) 357
PsuI RGATCY 2 cut(s) 29, 355
PvuI CGATCG 1 cut(s) 240
RseI CAYNNNNRTG 1 cut(s) 274
SaqAI TTAA 2 cut(s) 150, 177
Sau3AI GATC 3 cut(s) 29, 237, 355
ScrFI CCNGG 2 cut(s) 57, 229
SduI GDGCHC 1 cut(s) 14
SetI ASST 2 cut(s) 158, 291
SmiMI CAYNNNNRTG 1 cut(s) 274
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
Sse9I AATT 1 cut(s) 339
SspMI CTAG 2 cut(s) 201, 406
StyD4I CCNGG 2 cut(s) 55, 227
TaaI ACNGT 1 cut(s) 350
TaiI ACGT 1 cut(s) 158
TasI AATT 1 cut(s) 339
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 2 cut(s) 150, 177
Tru9I TTAA 2 cut(s) 150, 177
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 1 cut(s) 249
Tsp45I GTSAC 1 cut(s) 249
TspDTI ATGAA 4 cut(s) 30, 144, 148, 258
TspRI CASTG 1 cut(s) 406
XmiI GTMKAC 1 cut(s) 93
XspI CTAG 2 cut(s) 201, 406
Zsp2I ATGCAT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.