Rmu_co8338829.1_g000001

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8338829.1
OG0031206
Physical Location & Seq
Reverse (-)
1 .. 812
812 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8338829.1_g000001.1.cds

Sequence Viewer

Length: 686 bp
atgatatccttgttcgagttatcgaatgcagccacaacttataatgtgatgaaatatggtgcaaaagcagatgggaaaacagactcaacccaagcatttgtcaagacatgggcagcagcgtgcagctctgctcagacagccaccatgtacgttcccaaaggaaggttcttgctaaaagctgcagtgttcagaggcccttgcaagagtagaattaacgttcagatatacggaaccctgattgcccctactgattattgggctctgggaaactccggctattggatcttattcattaaggtcaatagacttgcagttttcggtggtacactcgacgccaagggagccggcttttgggcttgcaggaaatccggaaagagctgccctgttggagctaggtcgataacgttcaattgggcaaatgacgttgtgattagcggtttagtatccatcaacagtcagcaaactcatcttgtgatcaacagctgcaacaatgtagcggtcagaaacgttaagctggttgctccggatgacagccccaacactgatggcattcatgtgcagagctcaactggtgttactatcactggcgcaaccttgcaaaccggagacgattgtgtatcaattggtccgggcaccaaaaacctctacatgagtaacatcaagtgcggcccaggacatggcgtaag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

228

Amino Acids

24.15

Weight (kDa)

9.38

Isoelectric Point (pI)

26.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031206)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8338829.1_g000001 Rmu_sc0002352.1_g000019

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 42
AccB1I GGYRCC 1 cut(s) 632
AccB7I CCANNNNNTGG 1 cut(s) 677
AccIII TCCGGA 2 cut(s) 368, 523
AciI CCGC 3 cut(s) 435, 497, 666
AclI AACGTT 3 cut(s) 216, 404, 507
AclWI GGATC 1 cut(s) 290
AcyI GRCGYC 1 cut(s) 333
AfaI GTAC 2 cut(s) 149, 325
AfiI CCNNNNNNNGG 4 cut(s) 162, 279, 351, 677
AgsI TTSAA 1 cut(s) 409
AjnI CCWGG 1 cut(s) 670
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 7 cut(s) 126, 179, 378, 392, 483, 514, 564
AluI AGCT 7 cut(s) 126, 179, 378, 392, 483, 514, 564
Alw21I GWGCWC 1 cut(s) 566
Alw26I GTCTC 1 cut(s) 600
AlwI GGATC 1 cut(s) 290
Aor13HI TCCGGA 2 cut(s) 368, 523
AoxI GGCC 2 cut(s) 193, 667
ApeKI GCWGC 7 cut(s) 29, 113, 116, 123, 179, 378, 483
AspLEI GCGC 1 cut(s) 590
AspS9I GGNCC 3 cut(s) 194, 626, 668
AsuC2I CCSGG 1 cut(s) 630
AvaII GGWCC 1 cut(s) 626
BaeGI GKGCMC 1 cut(s) 635
BanI GGYRCC 1 cut(s) 632
BanII GRGCYC 2 cut(s) 262, 566
Bbv12I GWGCWC 1 cut(s) 566
BbvI GCAGC 7 cut(s) 41, 125, 128, 135, 166, 365, 470
BccI CCATC 3 cut(s) 65, 455, 539
BciT130I CCWGG 1 cut(s) 672
BciVI GTATCC 1 cut(s) 454
BclI TGATCA 1 cut(s) 474
BcnI CCSGG 1 cut(s) 630
BcoDI GTCTC 1 cut(s) 600
BfaI CTAG 1 cut(s) 393
BfmI CTRYAG 1 cut(s) 180
BfuI GTATCC 1 cut(s) 454
BisI GCNGC 8 cut(s) 30, 114, 117, 124, 180, 379, 484, 667
BlsI GCNGC 8 cut(s) 31, 115, 118, 125, 181, 380, 485, 668
Bme1390I CCNGG 2 cut(s) 630, 672
Bme18I GGWCC 1 cut(s) 626
BmgT120I GGNCC 3 cut(s) 194, 626, 668
BmiI GGNNCC 3 cut(s) 232, 343, 634
BmrFI CCNGG 2 cut(s) 630, 672
BpuMI CCSGG 1 cut(s) 630
BsaHI GRCGYC 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 336, 670
BsaWI WCCGGW 3 cut(s) 368, 523, 602
Bsc4I CCNNNNNNNGG 4 cut(s) 162, 279, 351, 677
Bse118I RCCGGY 1 cut(s) 344
Bse1I ACTGG 2 cut(s) 574, 589
BseAI TCCGGA 2 cut(s) 368, 523
BseBI CCWGG 1 cut(s) 672
BseDI CCNNGG 2 cut(s) 336, 670
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 4 cut(s) 162, 279, 351, 677
BseMII CTCAG 1 cut(s) 146
BseNI ACTGG 2 cut(s) 574, 589
BseSI GKGCMC 1 cut(s) 635
BseXI GCAGC 7 cut(s) 41, 125, 128, 135, 166, 365, 470
BsgI GTGCAG 2 cut(s) 142, 578
BshFI GGCC 2 cut(s) 195, 669
BshNI GGYRCC 1 cut(s) 632
BsiHKAI GWGCWC 1 cut(s) 566
BsiSI CCGG 6 cut(s) 273, 345, 369, 524, 603, 629
BslI CCNNNNNNNGG 4 cut(s) 162, 279, 351, 677
BsmAI GTCTC 1 cut(s) 600
BsmBI CGTCTC 1 cut(s) 600
BsmI GAATGC 2 cut(s) 31, 549
BsnI GGCC 2 cut(s) 195, 669
Bsp1286I GDGCHC 3 cut(s) 262, 566, 635
Bsp13I TCCGGA 2 cut(s) 368, 523
Bsp143I GATC 2 cut(s) 282, 474
BspACI CCGC 3 cut(s) 435, 497, 666
BspANI GGCC 2 cut(s) 195, 669
BspCNI CTCAG 1 cut(s) 145
BspEI TCCGGA 2 cut(s) 368, 523
BspLI GGNNCC 3 cut(s) 232, 343, 634
BspMAI CTGCAG 1 cut(s) 184
BspPI GGATC 1 cut(s) 290
BspT107I GGYRCC 1 cut(s) 632
BsrFI RCCGGY 1 cut(s) 344
BsrI ACTGG 2 cut(s) 574, 589
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 2 cut(s) 336, 670
BssMI GATC 2 cut(s) 282, 474
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 1 cut(s) 455
BstACI GRCGYC 1 cut(s) 333
BstC8I GCNNGC 3 cut(s) 121, 346, 358
BstDEI CTNAG 1 cut(s) 132
BstF5I GGATG 1 cut(s) 532
BstHHI GCGC 1 cut(s) 590
BstKTI GATC 2 cut(s) 285, 477
BstMAI GTCTC 1 cut(s) 600
BstMBI GATC 2 cut(s) 282, 474
BstMWI GCNNNNNNNGC 2 cut(s) 137, 341
BstNI CCWGG 1 cut(s) 672
BstSCI CCNGG 2 cut(s) 628, 670
BstSFI CTRYAG 1 cut(s) 180
BstSLI GKGCMC 1 cut(s) 635
BstV1I GCAGC 7 cut(s) 41, 125, 128, 135, 166, 365, 470
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
BsuI GTATCC 1 cut(s) 454
BsuRI GGCC 2 cut(s) 195, 669
BtsCI GGATG 1 cut(s) 532
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 3 cut(s) 189, 540, 582
Cac8I GCNNGC 3 cut(s) 121, 346, 358
CfoI GCGC 1 cut(s) 590
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 3 cut(s) 194, 626, 668
CseI GACGC 1 cut(s) 341
Csp6I GTAC 2 cut(s) 148, 324
CviAII CATG 5 cut(s) 108, 145, 554, 649, 677
CviQI GTAC 2 cut(s) 148, 324
DdeI CTNAG 1 cut(s) 132
DpnI GATC 2 cut(s) 284, 476
DpnII GATC 2 cut(s) 282, 474
Ecl136II GAGCTC 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 2 cut(s) 262, 566
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 1 cut(s) 626
Eco53kI GAGCTC 1 cut(s) 564
EcoICRI GAGCTC 1 cut(s) 564
EcoO109I RGGNCCY 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 670
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 336
EcoT38I GRGCYC 2 cut(s) 262, 566
ErhI CCWWGG 1 cut(s) 336
Esp3I CGTCTC 1 cut(s) 600
FaeI CATG 5 cut(s) 111, 148, 557, 652, 680
FaiI YATR 8 cut(s) 42, 57, 109, 146, 226, 555, 650, 678
FatI CATG 5 cut(s) 107, 144, 553, 648, 676
FbaI TGATCA 1 cut(s) 474
Fnu4HI GCNGC 8 cut(s) 30, 114, 117, 124, 180, 379, 484, 667
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 2 cut(s) 262, 566
Fsp4HI GCNGC 8 cut(s) 30, 114, 117, 124, 180, 379, 484, 667
FspBI CTAG 1 cut(s) 393
GlaI GCGC 1 cut(s) 589
GluI GCNGC 8 cut(s) 30, 114, 117, 124, 180, 379, 484, 667
HaeIII GGCC 2 cut(s) 195, 669
HapII CCGG 6 cut(s) 273, 345, 369, 524, 603, 629
HgaI GACGC 1 cut(s) 341
HhaI GCGC 1 cut(s) 590
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 5 cut(s) 111, 148, 557, 652, 680
Hin6I GCGC 1 cut(s) 588
HinP1I GCGC 1 cut(s) 588
HinfI GANTC 1 cut(s) 83
HpaII CCGG 6 cut(s) 273, 345, 369, 524, 603, 629
Hpy166II GTNNAC 1 cut(s) 326
Hpy188I TCNGA 4 cut(s) 135, 191, 222, 503
Hpy188III TCNNGA 3 cut(s) 103, 369, 524
Hpy8I GTNNAC 1 cut(s) 326
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 156
HpyCH4III ACNGT 1 cut(s) 455
HpyCH4IV ACGT 5 cut(s) 150, 216, 404, 423, 507
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 341
HpyF3I CTNAG 1 cut(s) 132
HpySE526I ACGT 5 cut(s) 150, 216, 404, 423, 507
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 5 cut(s) 111, 148, 557, 652, 680
HspAI GCGC 1 cut(s) 588
Kpn2I TCCGGA 2 cut(s) 368, 523
KroI GCCGGC 1 cut(s) 344
KroNI GCCGGC 1 cut(s) 346
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 2 cut(s) 282, 474
LmnI GCTCC 3 cut(s) 341, 389, 526
Lsp1109I GCAGC 7 cut(s) 41, 125, 128, 135, 166, 365, 470
MaeI CTAG 1 cut(s) 393
MaeII ACGT 5 cut(s) 150, 216, 404, 423, 507
MaeIII GTNAC 2 cut(s) 574, 653
MalI GATC 2 cut(s) 284, 476
MboI GATC 2 cut(s) 282, 474
MfeI CAATTG 2 cut(s) 409, 621
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 3 cut(s) 262, 566, 635
MluCI AATT 3 cut(s) 210, 409, 621
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 367
MnlI CCTC 2 cut(s) 185, 653
MroI TCCGGA 2 cut(s) 368, 523
MroNI GCCGGC 1 cut(s) 344
MseI TTAA 3 cut(s) 213, 294, 510
MslI CAYNNNNRTG 1 cut(s) 554
MspA1I CMGCKG 1 cut(s) 483
MspI CCGG 6 cut(s) 273, 345, 369, 524, 603, 629
MspR9I CCNGG 2 cut(s) 630, 672
MunI CAATTG 2 cut(s) 409, 621
Mva1269I GAATGC 2 cut(s) 31, 549
MvaI CCWGG 1 cut(s) 672
MwoI GCNNNNNNNGC 2 cut(s) 137, 341
NaeI GCCGGC 1 cut(s) 346
NciI CCSGG 1 cut(s) 630
NdeII GATC 2 cut(s) 282, 474
NgoMIV GCCGGC 1 cut(s) 344
NlaIII CATG 5 cut(s) 111, 148, 557, 652, 680
NlaIV GGNNCC 3 cut(s) 232, 343, 634
PctI GAATGC 2 cut(s) 31, 549
PdiI GCCGGC 1 cut(s) 346
PflMI CCANNNNNTGG 1 cut(s) 677
PkrI GCNGC 8 cut(s) 31, 115, 118, 125, 181, 380, 485, 668
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
PsiI TTATAA 1 cut(s) 42
Psp124BI GAGCTC 1 cut(s) 566
Psp1406I AACGTT 3 cut(s) 216, 404, 507
Psp6I CCWGG 1 cut(s) 670
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 3 cut(s) 232, 343, 634
PspPI GGNCC 3 cut(s) 194, 626, 668
PstI CTGCAG 1 cut(s) 184
PsuI RGATCY 1 cut(s) 282
PvuII CAGCTG 1 cut(s) 483
RsaI GTAC 2 cut(s) 149, 325
RsaNI GTAC 2 cut(s) 148, 324
RseI CAYNNNNRTG 1 cut(s) 554
SacI GAGCTC 1 cut(s) 566
SaqAI TTAA 3 cut(s) 213, 294, 510
SatI GCNGC 8 cut(s) 30, 114, 117, 124, 180, 379, 484, 667
Sau3AI GATC 2 cut(s) 282, 474
Sau96I GGNCC 3 cut(s) 194, 626, 668
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 630, 672
SduI GDGCHC 3 cut(s) 262, 566, 635
SfcI CTRYAG 1 cut(s) 180
SinI GGWCC 1 cut(s) 626
SmiMI CAYNNNNRTG 1 cut(s) 554
Sse9I AATT 3 cut(s) 210, 409, 621
SsiI CCGC 3 cut(s) 435, 497, 666
SspMI CTAG 1 cut(s) 393
SstI GAGCTC 1 cut(s) 566
StyD4I CCNGG 2 cut(s) 628, 670
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 1 cut(s) 455
TaiI ACGT 5 cut(s) 153, 219, 407, 426, 510
TaqI TCGA 4 cut(s) 15, 23, 330, 398
TasI AATT 3 cut(s) 210, 409, 621
TauI GCSGC 1 cut(s) 669
Tru1I TTAA 3 cut(s) 213, 294, 510
Tru9I TTAA 3 cut(s) 213, 294, 510
TscAI CASTG 3 cut(s) 189, 547, 589
TseI GCWGC 7 cut(s) 29, 113, 116, 123, 179, 378, 483
TspDTI ATGAA 3 cut(s) 65, 280, 542
TspGWI ACGGA 1 cut(s) 243
TspRI CASTG 3 cut(s) 189, 547, 589
Van91I CCANNNNNTGG 1 cut(s) 677
VpaK11BI GGWCC 1 cut(s) 626
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.