Rmu_sc0000083.1_g000035

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000083.1
OG0024991
Physical Location & Seq
Forward (+)
156703 .. 158906
2204 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000083.1_g000035.1.cds

Sequence Viewer

Length: 621 bp
atgttgccggcactgtccatggtgattggtggcctgcgttaccctcctccccttcatcaaaacaatgatgatgttttgataggttttgctgatgcaaggtacctctctgactctcacaaagcaaaagctcaatccccgccgcctcaggccaaaccgaattgcccagagcgctgcggtgacgtcccaattccatatccatttggcattggtgccgactgttacctccgaccagagttcaacatcacttgtgaccagtacaccaccatcactaaagttctccaacagtaccttcacctttcccgaattcccaccgattcttgcaatggtttcgggtgtggccaaaatgcaatccctactggactgcaaaacatgtcggtggatttgtatcaacttgacagctacatcagagattggaaccactggaacatagaaccagactacctgtgcagctatggcttcattgtggaagatggcgacttcacattctatgggaacacaagttttgaagaactgaacaccacaaggaggcagcttccggtggttgttaattgggcaattggggatgagacatgtgacgtagcaaagaagtcacagacttattcatgcaagcaaggaacatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

206

Amino Acids

23.12

Weight (kDa)

4.84

Isoelectric Point (pI)

55.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024991)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0016791
rosa_multiflora Rmu_sc0000083.1_g000035
rosa_roxburghii Rroxscaffold_3G00236260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 183
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 2 cut(s) 99, 209
AciI CCGC 3 cut(s) 137, 140, 174
AcoI YGGCCR 1 cut(s) 337
AcsI RAATTY 1 cut(s) 303
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 3 cut(s) 101, 257, 287
AfeI AGCGCT 1 cut(s) 170
AfiI CCNNNNNNNGG 1 cut(s) 525
AflIII ACRYGT 2 cut(s) 369, 569
AgsI TTSAA 2 cut(s) 238, 506
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 4 cut(s) 128, 399, 450, 532
AluI AGCT 4 cut(s) 128, 399, 450, 532
Alw26I GTCTC 1 cut(s) 560
Aor51HI AGCGCT 1 cut(s) 170
AoxI GGCC 3 cut(s) 31, 147, 337
ApeKI GCWGC 3 cut(s) 171, 447, 529
ApoI RAATTY 1 cut(s) 303
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 171
AsuHPI GGTGA 3 cut(s) 34, 188, 284
AxyI CCTNAGG 1 cut(s) 144
BalI TGGCCA 1 cut(s) 339
BanI GGYRCC 2 cut(s) 99, 209
BbvI GCAGC 3 cut(s) 158, 459, 541
BccI CCATC 2 cut(s) 272, 464
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BcoDI GTCTC 1 cut(s) 560
BfoI RGCGCY 1 cut(s) 172
BisI GCNGC 4 cut(s) 140, 172, 448, 530
BlsI GCNGC 4 cut(s) 141, 173, 449, 531
BmiI GGNNCC 3 cut(s) 101, 211, 416
BmsI GCATC 1 cut(s) 82
BsaBI GATNNNNATC 1 cut(s) 384
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 18
BsaWI WCCGGW 1 cut(s) 535
Bsc4I CCNNNNNNNGG 1 cut(s) 525
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 3 cut(s) 253, 361, 425
Bse21I CCTNAGG 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 328
Bse8I GATNNNNATC 1 cut(s) 384
BseDI CCNNGG 1 cut(s) 18
BseGI GGATG 1 cut(s) 568
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 1 cut(s) 525
BseMI GCAATG 1 cut(s) 328
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 3 cut(s) 253, 361, 425
BseRI GAGGAG 1 cut(s) 36
BseXI GCAGC 3 cut(s) 158, 459, 541
BsgI GTGCAG 1 cut(s) 466
BshFI GGCC 3 cut(s) 33, 149, 339
BshNI GGYRCC 2 cut(s) 99, 209
BsiSI CCGG 2 cut(s) 8, 536
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 1 cut(s) 525
BsmAI GTCTC 1 cut(s) 560
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 3 cut(s) 33, 149, 339
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 3 cut(s) 137, 140, 174
BspANI GGCC 3 cut(s) 33, 149, 339
BspCNI CTCAG 1 cut(s) 157
BspLI GGNNCC 3 cut(s) 101, 211, 416
BspT107I GGYRCC 2 cut(s) 99, 209
BsrDI GCAATG 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 3 cut(s) 253, 361, 425
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 1 cut(s) 18
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 18
Bst4CI ACNGT 3 cut(s) 15, 218, 285
BstACI GRCGYC 1 cut(s) 180
BstC8I GCNNGC 3 cut(s) 9, 35, 608
BstDEI CTNAG 1 cut(s) 144
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 568
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 1 cut(s) 171
BstMAI GTCTC 1 cut(s) 560
BstMWI GCNNNNNNNGC 2 cut(s) 168, 453
BstNSI RCATGY 2 cut(s) 373, 573
BstV1I GCAGC 3 cut(s) 158, 459, 541
Bsu36I CCTNAGG 1 cut(s) 144
BsuRI GGCC 3 cut(s) 33, 149, 339
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 1 cut(s) 568
BtsIMutI CAGTG 2 cut(s) 11, 418
Cac8I GCNNGC 3 cut(s) 9, 35, 608
CfoI GCGC 1 cut(s) 171
Cfr10I RCCGGY 1 cut(s) 7
Csp6I GTAC 3 cut(s) 100, 256, 286
CviAII CATG 4 cut(s) 19, 370, 570, 603
CviJI RGCY 8 cut(s) 33, 128, 149, 339, 399, 450, 456, 532
CviKI_1 RGCY 8 cut(s) 33, 128, 149, 339, 399, 450, 456, 532
CviQI GTAC 3 cut(s) 100, 256, 286
DdeI CTNAG 1 cut(s) 144
EaeI YGGCCR 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 18
Eco47III AGCGCT 1 cut(s) 170
Eco81I CCTNAGG 1 cut(s) 144
EcoRI GAATTC 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 4 cut(s) 22, 373, 573, 606
FaiI YATR 9 cut(s) 20, 193, 371, 428, 453, 489, 571, 604, 619
FaqI GGGAC 1 cut(s) 167
FatI CATG 4 cut(s) 18, 369, 569, 602
FauI CCCGC 1 cut(s) 144
Fnu4HI GCNGC 4 cut(s) 140, 172, 448, 530
FokI GGATG 1 cut(s) 575
Fsp4HI GCNGC 4 cut(s) 140, 172, 448, 530
GlaI GCGC 1 cut(s) 170
GluI GCNGC 4 cut(s) 140, 172, 448, 530
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 3 cut(s) 33, 149, 339
HapII CCGG 2 cut(s) 8, 536
HhaI GCGC 1 cut(s) 171
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 4 cut(s) 22, 373, 573, 606
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HinfI GANTC 2 cut(s) 110, 314
HpaII CCGG 2 cut(s) 8, 536
HphI GGTGA 3 cut(s) 34, 188, 284
Hpy166II GTNNAC 1 cut(s) 258
Hpy188I TCNGA 3 cut(s) 109, 227, 407
Hpy188III TCNNGA 1 cut(s) 300
Hpy8I GTNNAC 1 cut(s) 258
HpyAV CCTTC 2 cut(s) 62, 299
HpyCH4III ACNGT 3 cut(s) 15, 218, 285
HpyCH4IV ACGT 2 cut(s) 180, 576
HpyCH4V TGCA 6 cut(s) 95, 321, 347, 364, 447, 606
HpyF10VI GCNNNNNNNGC 2 cut(s) 168, 453
HpyF3I CTNAG 1 cut(s) 144
HpySE526I ACGT 2 cut(s) 180, 576
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 4 cut(s) 22, 373, 573, 606
HspAI GCGC 1 cut(s) 169
KpnI GGTACC 1 cut(s) 103
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
Lsp1109I GCAGC 3 cut(s) 158, 459, 541
LweI GCATC 1 cut(s) 82
MaeII ACGT 2 cut(s) 180, 576
MaeIII GTNAC 6 cut(s) 38, 176, 218, 248, 572, 588
MboII GAAGA 2 cut(s) 479, 518
MfeI CAATTG 1 cut(s) 555
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 5 cut(s) 157, 186, 303, 547, 555
MluNI TGGCCA 1 cut(s) 339
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 2 cut(s) 250, 304
MnlI CCTC 6 cut(s) 54, 57, 113, 153, 233, 519
Mox20I TGGCCA 1 cut(s) 339
MroNI GCCGGC 1 cut(s) 7
MscI TGGCCA 1 cut(s) 339
MseI TTAA 1 cut(s) 546
MslI CAYNNNNRTG 1 cut(s) 374
Msp20I TGGCCA 1 cut(s) 339
MspI CCGG 2 cut(s) 8, 536
MunI CAATTG 1 cut(s) 555
MwoI GCNNNNNNNGC 2 cut(s) 168, 453
NaeI GCCGGC 1 cut(s) 9
NcoI CCATGG 1 cut(s) 18
NgoMIV GCCGGC 1 cut(s) 7
NlaIII CATG 4 cut(s) 22, 373, 573, 606
NlaIV GGNNCC 3 cut(s) 101, 211, 416
NmuCI GTSAC 4 cut(s) 176, 248, 572, 588
NspI RCATGY 2 cut(s) 373, 573
PciI ACATGT 2 cut(s) 369, 569
PdiI GCCGGC 1 cut(s) 9
PfeI GAWTC 1 cut(s) 314
PkrI GCNGC 4 cut(s) 141, 173, 449, 531
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PscI ACATGT 2 cut(s) 369, 569
PspN4I GGNNCC 3 cut(s) 101, 211, 416
RsaI GTAC 3 cut(s) 101, 257, 287
RsaNI GTAC 3 cut(s) 100, 256, 286
RseI CAYNNNNRTG 1 cut(s) 374
SaqAI TTAA 1 cut(s) 546
SatI GCNGC 4 cut(s) 140, 172, 448, 530
SchI GAGTC 1 cut(s) 104
SfaNI GCATC 1 cut(s) 82
SmiMI CAYNNNNRTG 1 cut(s) 374
Sse9I AATT 5 cut(s) 157, 186, 303, 547, 555
SsiI CCGC 3 cut(s) 137, 140, 174
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 3 cut(s) 15, 218, 285
TaiI ACGT 2 cut(s) 183, 579
TasI AATT 5 cut(s) 157, 186, 303, 547, 555
TatI WGTACW 1 cut(s) 255
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 1 cut(s) 314
Tru1I TTAA 1 cut(s) 546
Tru9I TTAA 1 cut(s) 546
TscAI CASTG 2 cut(s) 18, 425
TseFI GTSAC 4 cut(s) 176, 248, 572, 588
TseI GCWGC 3 cut(s) 171, 447, 529
Tsp45I GTSAC 4 cut(s) 176, 248, 572, 588
TspDTI ATGAA 3 cut(s) 44, 448, 591
TspRI CASTG 2 cut(s) 18, 425
XapI RAATTY 1 cut(s) 303
XceI RCATGY 2 cut(s) 373, 573
ZraI GACGTC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.