Rroxscaffold_3G00236260

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0024991
Physical Location & Seq
Forward (+)
23450121 .. 23452404
2284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00236260.1

Sequence Viewer

Length: 522 bp
ATGTCGGTGGATTTGTATCAACTTGACAGCTACATTAGAGATTGGAACCACTGGAACATAGAGCCAGACTACCTGTGCAGCTATGGCTTCATTGTGGAAGATGGCGACTTCACATTCTCTGGGAACACAAGTTTTGAAGAACTGAACACCACAAGGAGGCAGCTTCCGGTGGTTGTTAATTGGGCAATTGGGGATGAGACATGTGACGTAGCAAAGAAGTCACAGACTTATTCATGCAAGCTAGGAACATGGCGACTTTGTGGATCGTACCATCAATGGTACTTCATCTGGAGTTTACACTTGCCGGTGCATGACAGGCTACGATGGGAACCCATACCTCTCAGATGGTTGCCAAGGGGTGGAAAGCCTGATTCTTCTCCTAAAGAGACTGACTACTTGCTTGCATCACCTTCAAACGCTTACGTTGTCGATGTTAAGGGTGAAGGCGATGGTGGTTCCATTACAACCAGTATTGAGTACGACCAGAGCATGCAAAATCAAGCCGTAGTGTTGAGGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

173

Amino Acids

19.95

Weight (kDa)

4.88

Isoelectric Point (pI)

54.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024991)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0016791
rosa_multiflora Rmu_sc0000083.1_g000035
rosa_roxburghii Rroxscaffold_3G00236260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 359
AclWI GGATC 1 cut(s) 271
AfaI GTAC 3 cut(s) 269, 281, 479
AfiI CCNNNNNNNGG 2 cut(s) 156, 359
AflIII ACRYGT 1 cut(s) 200
AgsI TTSAA 2 cut(s) 137, 414
AluBI AGCT 4 cut(s) 30, 81, 163, 241
AluI AGCT 4 cut(s) 30, 81, 163, 241
Alw26I GTCTC 2 cut(s) 191, 380
AlwI GGATC 1 cut(s) 271
ApeKI GCWGC 2 cut(s) 78, 160
AspS9I GGNCC 1 cut(s) 516
AsuHPI GGTGA 2 cut(s) 399, 452
AvaII GGWCC 1 cut(s) 516
BbvI GCAGC 2 cut(s) 90, 172
BccI CCATC 5 cut(s) 95, 279, 318, 339, 443
BceAI ACGGC 1 cut(s) 488
BcoDI GTCTC 2 cut(s) 191, 380
BfaI CTAG 1 cut(s) 242
BisI GCNGC 2 cut(s) 79, 161
BlsI GCNGC 2 cut(s) 80, 162
Bme18I GGWCC 1 cut(s) 516
BmgT120I GGNCC 1 cut(s) 516
BmiI GGNNCC 3 cut(s) 47, 330, 457
BmsI GCATC 1 cut(s) 413
BpmI CTGGAG 1 cut(s) 310
BsaBI GATNNNNATC 1 cut(s) 15
BsaJI CCNNGG 1 cut(s) 353
BsaWI WCCGGW 1 cut(s) 166
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 359
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 2 cut(s) 56, 468
Bse8I GATNNNNATC 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 353
BseGI GGATG 1 cut(s) 199
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 2 cut(s) 156, 359
BseMII CTCAG 1 cut(s) 355
BseNI ACTGG 2 cut(s) 56, 468
BseXI GCAGC 2 cut(s) 90, 172
BsgI GTGCAG 1 cut(s) 97
BsiSI CCGG 2 cut(s) 167, 305
BslI CCNNNNNNNGG 2 cut(s) 156, 359
BsmAI GTCTC 2 cut(s) 191, 380
Bsp143I GATC 1 cut(s) 263
BspCNI CTCAG 1 cut(s) 354
BspLI GGNNCC 3 cut(s) 47, 330, 457
BspPI GGATC 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 2 cut(s) 56, 468
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 353
BssMI GATC 1 cut(s) 263
BssT1I CCWWGG 1 cut(s) 353
BstC8I GCNNGC 3 cut(s) 239, 402, 491
BstDEI CTNAG 1 cut(s) 341
BstF5I GGATG 1 cut(s) 199
BstKTI GATC 1 cut(s) 266
BstMAI GTCTC 2 cut(s) 191, 380
BstMBI GATC 1 cut(s) 263
BstMWI GCNNNNNNNGC 2 cut(s) 84, 316
BstNSI RCATGY 2 cut(s) 204, 493
BstV1I GCAGC 2 cut(s) 90, 172
BtgZI GCGATG 1 cut(s) 462
BtsCI GGATG 1 cut(s) 199
BtsIMutI CAGTG 1 cut(s) 49
Cac8I GCNNGC 3 cut(s) 239, 402, 491
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 516
Csp6I GTAC 3 cut(s) 268, 280, 478
CviAII CATG 5 cut(s) 201, 234, 249, 311, 490
CviJI RGCY 9 cut(s) 30, 64, 81, 87, 163, 241, 319, 367, 503
CviKI_1 RGCY 9 cut(s) 30, 64, 81, 87, 163, 241, 319, 367, 503
CviQI GTAC 3 cut(s) 268, 280, 478
DdeI CTNAG 1 cut(s) 341
DpnI GATC 1 cut(s) 265
DpnII GATC 1 cut(s) 263
Eco130I CCWWGG 1 cut(s) 353
Eco47I GGWCC 1 cut(s) 516
EcoO109I RGGNCCY 1 cut(s) 516
EcoT14I CCWWGG 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 353
FaeI CATG 5 cut(s) 204, 237, 252, 314, 493
FaiI YATR 8 cut(s) 59, 84, 202, 235, 250, 312, 335, 491
FatI CATG 5 cut(s) 200, 233, 248, 310, 489
Fnu4HI GCNGC 2 cut(s) 79, 161
FokI GGATG 1 cut(s) 206
Fsp4HI GCNGC 2 cut(s) 79, 161
FspBI CTAG 1 cut(s) 242
GluI GCNGC 2 cut(s) 79, 161
GsuI CTGGAG 1 cut(s) 310
HapII CCGG 2 cut(s) 167, 305
Hin1II CATG 5 cut(s) 204, 237, 252, 314, 493
HinfI GANTC 1 cut(s) 371
HpaII CCGG 2 cut(s) 167, 305
HphI GGTGA 2 cut(s) 399, 452
Hpy166II GTNNAC 1 cut(s) 296
Hpy188I TCNGA 1 cut(s) 344
Hpy188III TCNNGA 1 cut(s) 289
Hpy8I GTNNAC 1 cut(s) 296
HpyAV CCTTC 2 cut(s) 420, 437
HpyCH4IV ACGT 2 cut(s) 207, 423
HpyCH4V TGCA 5 cut(s) 78, 237, 310, 404, 493
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 316
HpyF3I CTNAG 1 cut(s) 341
HpySE526I ACGT 2 cut(s) 207, 423
Hsp92II CATG 5 cut(s) 204, 237, 252, 314, 493
Kzo9I GATC 1 cut(s) 263
Lsp1109I GCAGC 2 cut(s) 90, 172
LweI GCATC 1 cut(s) 413
MaeI CTAG 1 cut(s) 242
MaeII ACGT 2 cut(s) 207, 423
MaeIII GTNAC 2 cut(s) 203, 219
MalI GATC 1 cut(s) 265
MboI GATC 1 cut(s) 263
MboII GAAGA 3 cut(s) 110, 149, 366
MfeI CAATTG 1 cut(s) 186
MluCI AATT 2 cut(s) 178, 186
MnlI CCTC 3 cut(s) 150, 348, 507
MseI TTAA 2 cut(s) 177, 435
MspI CCGG 2 cut(s) 167, 305
MunI CAATTG 1 cut(s) 186
MwoI GCNNNNNNNGC 2 cut(s) 84, 316
NdeII GATC 1 cut(s) 263
NlaIII CATG 5 cut(s) 204, 237, 252, 314, 493
NlaIV GGNNCC 3 cut(s) 47, 330, 457
NmuCI GTSAC 2 cut(s) 203, 219
NspI RCATGY 2 cut(s) 204, 493
PaeI GCATGC 1 cut(s) 493
PciI ACATGT 1 cut(s) 200
PfeI GAWTC 1 cut(s) 371
PflMI CCANNNNNTGG 1 cut(s) 359
PkrI GCNGC 2 cut(s) 80, 162
PpuMI RGGWCCY 1 cut(s) 516
PscI ACATGT 1 cut(s) 200
Psp5II RGGWCCY 1 cut(s) 516
PspN4I GGNNCC 3 cut(s) 47, 330, 457
PspPI GGNCC 1 cut(s) 516
PspPPI RGGWCCY 1 cut(s) 516
RsaI GTAC 3 cut(s) 269, 281, 479
RsaNI GTAC 3 cut(s) 268, 280, 478
SaqAI TTAA 2 cut(s) 177, 435
SatI GCNGC 2 cut(s) 79, 161
Sau3AI GATC 1 cut(s) 263
Sau96I GGNCC 1 cut(s) 516
SfaNI GCATC 1 cut(s) 413
SinI GGWCC 1 cut(s) 516
SphI GCATGC 1 cut(s) 493
Sse9I AATT 2 cut(s) 178, 186
SspMI CTAG 1 cut(s) 242
StyI CCWWGG 1 cut(s) 353
TaiI ACGT 2 cut(s) 210, 426
TaqI TCGA 1 cut(s) 429
TasI AATT 2 cut(s) 178, 186
TfiI GAWTC 1 cut(s) 371
Tru1I TTAA 2 cut(s) 177, 435
Tru9I TTAA 2 cut(s) 177, 435
TscAI CASTG 1 cut(s) 56
TseFI GTSAC 2 cut(s) 203, 219
TseI GCWGC 2 cut(s) 78, 160
Tsp45I GTSAC 2 cut(s) 203, 219
TspDTI ATGAA 3 cut(s) 79, 222, 274
TspRI CASTG 1 cut(s) 56
Van91I CCANNNNNTGG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 516
XceI RCATGY 2 cut(s) 204, 493
XspI CTAG 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.