Rmu_sc0000083.1_g000035

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000083.1
OG0024991
Physical Location & Seq
Forward (+)
156703 .. 158906
2204 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 621 bp
atgttgccggcactgtccatggtgattggtggcctgcgttaccctcctccccttcatcaaaacaatgatgatgttttgataggttttgctgatgcaaggtacctctctgactctcacaaagcaaaagctcaatccccgccgcctcaggccaaaccgaattgcccagagcgctgcggtgacgtcccaattccatatccatttggcattggtgccgactgttacctccgaccagagttcaacatcacttgtgaccagtacaccaccatcactaaagttctccaacagtaccttcacctttcccgaattcccaccgattcttgcaatggtttcgggtgtggccaaaatgcaatccctactggactgcaaaacatgtcggtggatttgtatcaacttgacagctacatcagagattggaaccactggaacatagaaccagactacctgtgcagctatggcttcattgtggaagatggcgacttcacattctatgggaacacaagttttgaagaactgaacaccacaaggaggcagcttccggtggttgttaattgggcaattggggatgagacatgtgacgtagcaaagaagtcacagacttattcatgcaagcaaggaacatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

206

Amino Acids

23.12

Weight (kDa)

4.84

Isoelectric Point (pI)

55.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 54 - 90 7.5e-10 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024991)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0016791
rosa_multiflora Rmu_sc0000083.1_g000035
rosa_roxburghii Rroxscaffold_3G00236260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 183
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 2 cut(s) 99, 209
AciI CCGC 3 cut(s) 137, 140, 174
AcoI YGGCCR 1 cut(s) 337
AcsI RAATTY 1 cut(s) 303
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 3 cut(s) 101, 257, 287
AfeI AGCGCT 1 cut(s) 170
AfiI CCNNNNNNNGG 1 cut(s) 525
AflIII ACRYGT 2 cut(s) 369, 569
AgsI TTSAA 2 cut(s) 238, 506
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 4 cut(s) 128, 399, 450, 532
AluI AGCT 4 cut(s) 128, 399, 450, 532
Alw26I GTCTC 1 cut(s) 560
Aor51HI AGCGCT 1 cut(s) 170
AoxI GGCC 3 cut(s) 31, 147, 337
ApeKI GCWGC 3 cut(s) 171, 447, 529
ApoI RAATTY 1 cut(s) 303
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 171
AsuHPI GGTGA 3 cut(s) 34, 188, 284
AxyI CCTNAGG 1 cut(s) 144
BalI TGGCCA 1 cut(s) 339
BanI GGYRCC 2 cut(s) 99, 209
BbvI GCAGC 3 cut(s) 158, 459, 541
BccI CCATC 2 cut(s) 272, 464
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BcoDI GTCTC 1 cut(s) 560
BfoI RGCGCY 1 cut(s) 172
BisI GCNGC 4 cut(s) 140, 172, 448, 530
BlsI GCNGC 4 cut(s) 141, 173, 449, 531
BmiI GGNNCC 3 cut(s) 101, 211, 416
BmsI GCATC 1 cut(s) 82
BsaBI GATNNNNATC 1 cut(s) 384
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 18
BsaWI WCCGGW 1 cut(s) 535
Bsc4I CCNNNNNNNGG 1 cut(s) 525
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 3 cut(s) 253, 361, 425
Bse21I CCTNAGG 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 328
Bse8I GATNNNNATC 1 cut(s) 384
BseDI CCNNGG 1 cut(s) 18
BseGI GGATG 1 cut(s) 568
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 1 cut(s) 525
BseMI GCAATG 1 cut(s) 328
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 3 cut(s) 253, 361, 425
BseRI GAGGAG 1 cut(s) 36
BseXI GCAGC 3 cut(s) 158, 459, 541
BsgI GTGCAG 1 cut(s) 466
BshFI GGCC 3 cut(s) 33, 149, 339
BshNI GGYRCC 2 cut(s) 99, 209
BsiSI CCGG 2 cut(s) 8, 536
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 1 cut(s) 525
BsmAI GTCTC 1 cut(s) 560
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 3 cut(s) 33, 149, 339
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 3 cut(s) 137, 140, 174
BspANI GGCC 3 cut(s) 33, 149, 339
BspCNI CTCAG 1 cut(s) 157
BspLI GGNNCC 3 cut(s) 101, 211, 416
BspT107I GGYRCC 2 cut(s) 99, 209
BsrDI GCAATG 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 3 cut(s) 253, 361, 425
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 1 cut(s) 18
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 18
Bst4CI ACNGT 3 cut(s) 15, 218, 285
BstACI GRCGYC 1 cut(s) 180
BstC8I GCNNGC 3 cut(s) 9, 35, 608
BstDEI CTNAG 1 cut(s) 144
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 568
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 1 cut(s) 171
BstMAI GTCTC 1 cut(s) 560
BstMWI GCNNNNNNNGC 2 cut(s) 168, 453
BstNSI RCATGY 2 cut(s) 373, 573
BstV1I GCAGC 3 cut(s) 158, 459, 541
Bsu36I CCTNAGG 1 cut(s) 144
BsuRI GGCC 3 cut(s) 33, 149, 339
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 1 cut(s) 568
BtsIMutI CAGTG 2 cut(s) 11, 418
Cac8I GCNNGC 3 cut(s) 9, 35, 608
CfoI GCGC 1 cut(s) 171
Cfr10I RCCGGY 1 cut(s) 7
Csp6I GTAC 3 cut(s) 100, 256, 286
CviAII CATG 4 cut(s) 19, 370, 570, 603
CviJI RGCY 8 cut(s) 33, 128, 149, 339, 399, 450, 456, 532
CviKI_1 RGCY 8 cut(s) 33, 128, 149, 339, 399, 450, 456, 532
CviQI GTAC 3 cut(s) 100, 256, 286
DdeI CTNAG 1 cut(s) 144
EaeI YGGCCR 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 18
Eco47III AGCGCT 1 cut(s) 170
Eco81I CCTNAGG 1 cut(s) 144
EcoRI GAATTC 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 4 cut(s) 22, 373, 573, 606
FaiI YATR 9 cut(s) 20, 193, 371, 428, 453, 489, 571, 604, 619
FaqI GGGAC 1 cut(s) 167
FatI CATG 4 cut(s) 18, 369, 569, 602
FauI CCCGC 1 cut(s) 144
Fnu4HI GCNGC 4 cut(s) 140, 172, 448, 530
FokI GGATG 1 cut(s) 575
Fsp4HI GCNGC 4 cut(s) 140, 172, 448, 530
GlaI GCGC 1 cut(s) 170
GluI GCNGC 4 cut(s) 140, 172, 448, 530
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 3 cut(s) 33, 149, 339
HapII CCGG 2 cut(s) 8, 536
HhaI GCGC 1 cut(s) 171
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 4 cut(s) 22, 373, 573, 606
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HinfI GANTC 2 cut(s) 110, 314
HpaII CCGG 2 cut(s) 8, 536
HphI GGTGA 3 cut(s) 34, 188, 284
Hpy166II GTNNAC 1 cut(s) 258
Hpy188I TCNGA 3 cut(s) 109, 227, 407
Hpy188III TCNNGA 1 cut(s) 300
Hpy8I GTNNAC 1 cut(s) 258
HpyAV CCTTC 2 cut(s) 62, 299
HpyCH4III ACNGT 3 cut(s) 15, 218, 285
HpyCH4IV ACGT 2 cut(s) 180, 576
HpyCH4V TGCA 6 cut(s) 95, 321, 347, 364, 447, 606
HpyF10VI GCNNNNNNNGC 2 cut(s) 168, 453
HpyF3I CTNAG 1 cut(s) 144
HpySE526I ACGT 2 cut(s) 180, 576
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 4 cut(s) 22, 373, 573, 606
HspAI GCGC 1 cut(s) 169
KpnI GGTACC 1 cut(s) 103
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
Lsp1109I GCAGC 3 cut(s) 158, 459, 541
LweI GCATC 1 cut(s) 82
MaeII ACGT 2 cut(s) 180, 576
MaeIII GTNAC 6 cut(s) 38, 176, 218, 248, 572, 588
MboII GAAGA 2 cut(s) 479, 518
MfeI CAATTG 1 cut(s) 555
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 5 cut(s) 157, 186, 303, 547, 555
MluNI TGGCCA 1 cut(s) 339
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 2 cut(s) 250, 304
MnlI CCTC 6 cut(s) 54, 57, 113, 153, 233, 519
Mox20I TGGCCA 1 cut(s) 339
MroNI GCCGGC 1 cut(s) 7
MscI TGGCCA 1 cut(s) 339
MseI TTAA 1 cut(s) 546
MslI CAYNNNNRTG 1 cut(s) 374
Msp20I TGGCCA 1 cut(s) 339
MspI CCGG 2 cut(s) 8, 536
MunI CAATTG 1 cut(s) 555
MwoI GCNNNNNNNGC 2 cut(s) 168, 453
NaeI GCCGGC 1 cut(s) 9
NcoI CCATGG 1 cut(s) 18
NgoMIV GCCGGC 1 cut(s) 7
NlaIII CATG 4 cut(s) 22, 373, 573, 606
NlaIV GGNNCC 3 cut(s) 101, 211, 416
NmuCI GTSAC 4 cut(s) 176, 248, 572, 588
NspI RCATGY 2 cut(s) 373, 573
PciI ACATGT 2 cut(s) 369, 569
PdiI GCCGGC 1 cut(s) 9
PfeI GAWTC 1 cut(s) 314
PkrI GCNGC 4 cut(s) 141, 173, 449, 531
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PscI ACATGT 2 cut(s) 369, 569
PspN4I GGNNCC 3 cut(s) 101, 211, 416
RsaI GTAC 3 cut(s) 101, 257, 287
RsaNI GTAC 3 cut(s) 100, 256, 286
RseI CAYNNNNRTG 1 cut(s) 374
SaqAI TTAA 1 cut(s) 546
SatI GCNGC 4 cut(s) 140, 172, 448, 530
SchI GAGTC 1 cut(s) 104
SfaNI GCATC 1 cut(s) 82
SmiMI CAYNNNNRTG 1 cut(s) 374
Sse9I AATT 5 cut(s) 157, 186, 303, 547, 555
SsiI CCGC 3 cut(s) 137, 140, 174
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 3 cut(s) 15, 218, 285
TaiI ACGT 2 cut(s) 183, 579
TasI AATT 5 cut(s) 157, 186, 303, 547, 555
TatI WGTACW 1 cut(s) 255
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 1 cut(s) 314
Tru1I TTAA 1 cut(s) 546
Tru9I TTAA 1 cut(s) 546
TscAI CASTG 2 cut(s) 18, 425
TseFI GTSAC 4 cut(s) 176, 248, 572, 588
TseI GCWGC 3 cut(s) 171, 447, 529
Tsp45I GTSAC 4 cut(s) 176, 248, 572, 588
TspDTI ATGAA 3 cut(s) 44, 448, 591
TspRI CASTG 2 cut(s) 18, 425
XapI RAATTY 1 cut(s) 303
XceI RCATGY 2 cut(s) 373, 573
ZraI GACGTC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.