Rmu_sc0000149.1_g000050

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000149.1
OG0031045
Physical Location & Seq
Forward (+)
190270 .. 190980
711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000149.1_g000050.1.cds

Sequence Viewer

Length: 711 bp
atgaaactcgacccgagaacaattgatcaatctgttggtctgtatgctagggttctagttgacattgacctttcaaagcctttggttcagcagcttcgagtgacccgtgtaaatggagaccaggtttcagttggcgttgagtacgaagcccttccaagcatatgttccaagtgcggccttgttgggcacttagaggtcaaatgcagagtaatggcccgagctcctatagatggggtgcatatctccggtcgtggtcactctaccactcatagatcgaggaggaagtccaaggacgtttctggtcctcccactcgcagtggagctgctccgccatgcaatgtggatgcaattaatgttagtgagagtaatggtttggtggataagaacagttcaacaagccagatggatgttaatctaatggcaagtgttgtgaagtctggtgccatccttaagacaattgtggtgaatgatggtgcaaaaaatggcttggttatgcagcaaacttctaaccaggcttcttcctttgagagtgtcccacctggctttcagaggctacccacgggaagtttgaaggaggctaagacagttagtattcatcagatagtgttactaagtcgccaattagtgacttgggaagaggggcagtttcaatccctcttaagaatgttgcggactcggaggaccagatggaggatggtgagttcacactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

236

Amino Acids

25.74

Weight (kDa)

10.02

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031045)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000149.1_g000050
rosa_samantha Rh5DG258800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 440
AciI CCGC 3 cut(s) 174, 329, 670
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 2 cut(s) 230, 690
AflII CTTAAG 2 cut(s) 449, 658
AgsI TTSAA 4 cut(s) 75, 393, 571, 650
AjnI CCWGG 3 cut(s) 120, 510, 538
AjuI GAANNNNNNNTTGG 2 cut(s) 148, 180
AloI GAACNNNNNNTCC 1 cut(s) 685
AluBI AGCT 3 cut(s) 94, 221, 323
AluI AGCT 3 cut(s) 94, 221, 323
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 111
Ama87I CYCGRG 2 cut(s) 13, 216
AoxI GGCC 2 cut(s) 175, 213
ApeKI GCWGC 3 cut(s) 91, 323, 496
AseI ATTAAT 1 cut(s) 351
Asp700I GAANNNNTTC 1 cut(s) 150
AspS9I GGNCC 3 cut(s) 214, 302, 681
AsuHPI GGTGA 2 cut(s) 475, 709
AvaI CYCGRG 2 cut(s) 13, 216
AvaII GGWCC 2 cut(s) 302, 681
BaeGI GKGCMC 1 cut(s) 189
BanI GGYRCC 1 cut(s) 440
BanII GRGCYC 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 3 cut(s) 103, 310, 508
BccI CCATC 6 cut(s) 224, 397, 452, 464, 681, 688
BciT130I CCWGG 3 cut(s) 122, 512, 540
BclI TGATCA 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 3 cut(s) 48, 56, 709
BfmI CTRYAG 1 cut(s) 225
BfrI CTTAAG 2 cut(s) 449, 658
BisI GCNGC 4 cut(s) 92, 175, 324, 497
BlsI GCNGC 4 cut(s) 93, 176, 325, 498
Bme1390I CCNGG 3 cut(s) 122, 512, 540
Bme18I GGWCC 2 cut(s) 302, 681
BmeT110I CYCGRG 2 cut(s) 13, 216
BmgT120I GGNCC 3 cut(s) 214, 302, 681
BmiI GGNNCC 1 cut(s) 442
BmrFI CCNGG 3 cut(s) 122, 512, 540
BmsI GCATC 1 cut(s) 334
BsaBI GATNNNNATC 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 111
BsaJI CCNNGG 2 cut(s) 288, 558
BsaWI WCCGGW 1 cut(s) 245
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 690
Bse3DI GCAATG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 3 cut(s) 122, 512, 540
BseDI CCNNGG 2 cut(s) 288, 558
BseGI GGATG 4 cut(s) 349, 412, 444, 699
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 2 cut(s) 230, 690
BseMI GCAATG 1 cut(s) 343
BseRI GAGGAG 1 cut(s) 292
BseSI GKGCMC 1 cut(s) 189
BseXI GCAGC 3 cut(s) 103, 310, 508
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 2 cut(s) 177, 215
BshNI GGYRCC 1 cut(s) 440
BsiEI CGRYCG 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 223
BsiHKCI CYCGRG 2 cut(s) 13, 216
BsiSI CCGG 1 cut(s) 246
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 2 cut(s) 230, 690
BsmAI GTCTC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 2 cut(s) 177, 215
Bso31I GGTCTC 1 cut(s) 111
BsoBI CYCGRG 2 cut(s) 13, 216
Bsp1286I GDGCHC 2 cut(s) 189, 223
Bsp143I GATC 2 cut(s) 25, 272
BspACI CCGC 3 cut(s) 174, 329, 670
BspANI GGCC 2 cut(s) 177, 215
BspLI GGNNCC 1 cut(s) 442
BspT107I GGYRCC 1 cut(s) 440
BspTI CTTAAG 2 cut(s) 449, 658
BspTNI GGTCTC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 343
BssECI CCNNGG 2 cut(s) 288, 558
BssMI GATC 2 cut(s) 25, 272
BssT1I CCWWGG 1 cut(s) 288
Bst2UI CCWGG 3 cut(s) 122, 512, 540
Bst4CI ACNGT 2 cut(s) 389, 586
Bst6I CTCTTC 1 cut(s) 630
BstAFI CTTAAG 2 cut(s) 449, 658
BstDEI CTNAG 3 cut(s) 190, 579, 611
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 4 cut(s) 349, 412, 444, 699
BstKTI GATC 2 cut(s) 28, 275
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 2 cut(s) 25, 272
BstMCI CGRYCG 1 cut(s) 250
BstNI CCWGG 3 cut(s) 122, 512, 540
BstSCI CCNGG 3 cut(s) 120, 510, 538
BstSFI CTRYAG 1 cut(s) 225
BstSLI GKGCMC 1 cut(s) 189
BstV1I GCAGC 3 cut(s) 103, 310, 508
BsuRI GGCC 2 cut(s) 177, 215
BtgI CCRYGG 1 cut(s) 558
BtsCI GGATG 4 cut(s) 349, 412, 444, 699
BtsI GCAGTG 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 322
Cfr13I GGNCC 3 cut(s) 214, 302, 681
CsiI ACCWGGT 1 cut(s) 120
Csp6I GTAC 1 cut(s) 142
CviAII CATG 1 cut(s) 333
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 3 cut(s) 190, 579, 611
DpnI GATC 2 cut(s) 27, 274
DpnII GATC 2 cut(s) 25, 272
Eam1104I CTCTTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 630
EciI GGCGGA 1 cut(s) 318
Ecl136II GAGCTC 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 223
Eco31I GGTCTC 1 cut(s) 111
Eco47I GGWCC 2 cut(s) 302, 681
Eco53kI GAGCTC 1 cut(s) 221
Eco88I CYCGRG 2 cut(s) 13, 216
EcoICRI GAGCTC 1 cut(s) 221
EcoRII CCWGG 3 cut(s) 120, 510, 538
EcoT14I CCWWGG 1 cut(s) 288
EcoT38I GRGCYC 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 1 cut(s) 336
FaiI YATR 8 cut(s) 45, 161, 163, 227, 240, 270, 334, 494
FaqI GGGAC 1 cut(s) 518
FatI CATG 1 cut(s) 332
FauNDI CATATG 1 cut(s) 161
FbaI TGATCA 1 cut(s) 25
Fnu4HI GCNGC 4 cut(s) 92, 175, 324, 497
FokI GGATG 4 cut(s) 356, 419, 431, 706
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 4 cut(s) 92, 175, 324, 497
FspBI CTAG 3 cut(s) 48, 56, 709
GluI GCNGC 4 cut(s) 92, 175, 324, 497
HaeIII GGCC 2 cut(s) 177, 215
HapII CCGG 1 cut(s) 246
Hin1II CATG 1 cut(s) 336
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 1 cut(s) 673
HpaII CCGG 1 cut(s) 246
HphI GGTGA 2 cut(s) 475, 709
Hpy166II GTNNAC 2 cut(s) 61, 704
Hpy188I TCNGA 3 cut(s) 549, 600, 678
Hpy8I GTNNAC 2 cut(s) 61, 704
HpyAV CCTTC 2 cut(s) 161, 565
HpyCH4III ACNGT 2 cut(s) 389, 586
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 6 cut(s) 204, 238, 336, 347, 476, 496
HpyF3I CTNAG 3 cut(s) 190, 579, 611
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 1 cut(s) 336
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 2 cut(s) 25, 272
LmnI GCTCC 3 cut(s) 226, 320, 331
Lsp1109I GCAGC 3 cut(s) 103, 310, 508
LweI GCATC 1 cut(s) 334
MabI ACCWGGT 1 cut(s) 120
MaeI CTAG 3 cut(s) 48, 56, 709
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 4 cut(s) 100, 254, 606, 625
MalI GATC 2 cut(s) 27, 274
MboI GATC 2 cut(s) 25, 272
MboII GAAGA 2 cut(s) 510, 647
MfeI CAATTG 2 cut(s) 21, 456
MhlI GDGCHC 2 cut(s) 189, 223
MluCI AATT 4 cut(s) 21, 348, 456, 620
MlyI GAGTC 1 cut(s) 667
MroXI GAANNNNTTC 1 cut(s) 150
MseI TTAA 4 cut(s) 351, 411, 450, 659
MspCI CTTAAG 2 cut(s) 449, 658
MspI CCGG 1 cut(s) 246
MspR9I CCNGG 3 cut(s) 122, 512, 540
MunI CAATTG 2 cut(s) 21, 456
MvaI CCWGG 3 cut(s) 122, 512, 540
NdeI CATATG 1 cut(s) 161
NdeII GATC 2 cut(s) 25, 272
NlaIII CATG 1 cut(s) 336
NlaIV GGNNCC 1 cut(s) 442
NmuCI GTSAC 3 cut(s) 100, 254, 625
PcsI WCGNNNNNNNCGW 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 150
PkrI GCNGC 4 cut(s) 93, 176, 325, 498
PleI GAGTC 1 cut(s) 667
PpsI GAGTC 1 cut(s) 667
PshBI ATTAAT 1 cut(s) 351
Psp124BI GAGCTC 1 cut(s) 223
Psp6I CCWGG 3 cut(s) 120, 510, 538
PspGI CCWGG 3 cut(s) 120, 510, 538
PspN4I GGNNCC 1 cut(s) 442
PspPI GGNCC 3 cut(s) 214, 302, 681
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SacI GAGCTC 1 cut(s) 223
SaqAI TTAA 4 cut(s) 351, 411, 450, 659
SatI GCNGC 4 cut(s) 92, 175, 324, 497
Sau3AI GATC 2 cut(s) 25, 272
Sau96I GGNCC 3 cut(s) 214, 302, 681
SchI GAGTC 1 cut(s) 667
ScrFI CCNGG 3 cut(s) 122, 512, 540
SduI GDGCHC 2 cut(s) 189, 223
SetI ASST 8 cut(s) 72, 96, 126, 198, 223, 297, 325, 541
SexAI ACCWGGT 1 cut(s) 120
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 225
SinI GGWCC 2 cut(s) 302, 681
SmlI CTYRAG 2 cut(s) 449, 658
SmoI CTYRAG 2 cut(s) 449, 658
Sse9I AATT 4 cut(s) 21, 348, 456, 620
SsiI CCGC 3 cut(s) 174, 329, 670
SspMI CTAG 3 cut(s) 48, 56, 709
SstI GAGCTC 1 cut(s) 223
StyD4I CCNGG 3 cut(s) 120, 510, 538
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 2 cut(s) 389, 586
TaiI ACGT 1 cut(s) 297
TaqI TCGA 3 cut(s) 9, 97, 275
TasI AATT 4 cut(s) 21, 348, 456, 620
TauI GCSGC 1 cut(s) 177
Tru1I TTAA 4 cut(s) 351, 411, 450, 659
Tru9I TTAA 4 cut(s) 351, 411, 450, 659
TscAI CASTG 1 cut(s) 322
TseFI GTSAC 3 cut(s) 100, 254, 625
TseI GCWGC 3 cut(s) 91, 323, 496
Tsp45I GTSAC 3 cut(s) 100, 254, 625
TspDTI ATGAA 2 cut(s) 17, 584
TspRI CASTG 1 cut(s) 322
Vha464I CTTAAG 2 cut(s) 449, 658
VpaK11BI GGWCC 2 cut(s) 302, 681
VspI ATTAAT 1 cut(s) 351
XcmI CCANNNNNNNNNTGG 2 cut(s) 128, 691
XmnI GAANNNNTTC 1 cut(s) 150
XspI CTAG 3 cut(s) 48, 56, 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.