Rmu_sc0000317.1_g000035

Bacterial Ig-like domain 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000317.1
OG0030166
Physical Location & Seq
Forward (+)
195055 .. 195531
477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000317.1_g000035.1.cds

Sequence Viewer

Length: 477 bp
atgaaactaaattttgttcttagggaaggcctcaagctcgacaataagatggcagtgtgcttcaccgacagccaatcacgatcgcatctattaaagtggttcattctttgctctgagctttgtcattgtgagactaaactgttttgcaaggaaccattcgacggacttggtgaactgactcaaaactcattgtcgcagcttatttctacattaagggttagtccaaatcttcattcagtagttttcattcccgatgctaaggtaaatctgggaattactagttcaatctgttttctaaaagttgttgtaaatgatagtcaagtagtggaagtcattcaacgcccaacatatttacaatgctcccaactggtgttgtctcctaaaggcttggggactgcgcttgtgatagtttatgatattgggattgctcctccacttgcatcttctgatgtggtacaagttgcagagattgcctga

Protein Analysis

158

Amino Acids

17.35

Weight (kDa)

6.27

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0030166)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0056391
rosa_multiflora Rmu_sc0000317.1_g000035

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 10
AfaI GTAC 1 cut(s) 456
AfiI CCNNNNNNNGG 1 cut(s) 161
AgsI TTSAA 2 cut(s) 285, 338
AhlI ACTAGT 1 cut(s) 278
AjuI GAANNNNNNNTTGG 2 cut(s) 217, 249
AluBI AGCT 3 cut(s) 37, 118, 199
AluI AGCT 3 cut(s) 37, 118, 199
Alw26I GTCTC 2 cut(s) 125, 381
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 1 cut(s) 196
ApoI RAATTY 1 cut(s) 10
Asp700I GAANNNNTTC 1 cut(s) 333
AspLEI GCGC 1 cut(s) 400
AsuHPI GGTGA 2 cut(s) 55, 182
BbvI GCAGC 1 cut(s) 208
BccI CCATC 1 cut(s) 43
BcoDI GTCTC 2 cut(s) 125, 381
BcuI ACTAGT 1 cut(s) 278
BfaI CTAG 1 cut(s) 279
BisI GCNGC 1 cut(s) 197
BlsI GCNGC 1 cut(s) 198
BmiI GGNNCC 1 cut(s) 153
BmsI GCATC 3 cut(s) 94, 244, 449
Bpu10I CCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 17
Bsc4I CCNNNNNNNGG 1 cut(s) 161
Bse1I ACTGG 1 cut(s) 372
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 1 cut(s) 372
BseRI GAGGAG 1 cut(s) 420
BseXI GCAGC 1 cut(s) 208
Bsh1285I CGRYCG 1 cut(s) 83
BshFI GGCC 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 83
BslFI GGGAC 1 cut(s) 406
BslI CCNNNNNNNGG 1 cut(s) 161
BsmAI GTCTC 2 cut(s) 125, 381
BsmFI GGGAC 1 cut(s) 406
BsnI GGCC 1 cut(s) 30
Bsp143I GATC 1 cut(s) 80
BspANI GGCC 1 cut(s) 30
BspCNI CTCAG 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 153
BsrI ACTGG 1 cut(s) 372
BssMI GATC 1 cut(s) 80
Bst4CI ACNGT 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 470
BstDEI CTNAG 3 cut(s) 20, 114, 258
BstHHI GCGC 1 cut(s) 400
BstKTI GATC 1 cut(s) 83
BstMAI GTCTC 2 cut(s) 125, 381
BstMBI GATC 1 cut(s) 80
BstMCI CGRYCG 1 cut(s) 83
BstMWI GCNNNNNNNGC 1 cut(s) 470
BstV1I GCAGC 1 cut(s) 208
BsuRI GGCC 1 cut(s) 30
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 1 cut(s) 60
CfoI GCGC 1 cut(s) 400
Csp6I GTAC 1 cut(s) 455
CviJI RGCY 6 cut(s) 30, 37, 72, 118, 199, 387
CviKI_1 RGCY 6 cut(s) 30, 37, 72, 118, 199, 387
CviQI GTAC 1 cut(s) 455
DdeI CTNAG 3 cut(s) 20, 114, 258
DpnI GATC 1 cut(s) 82
DpnII GATC 1 cut(s) 80
Eco147I AGGCCT 1 cut(s) 30
FaiI YATR 2 cut(s) 349, 414
FaqI GGGAC 1 cut(s) 406
Fnu4HI GCNGC 1 cut(s) 197
Fsp4HI GCNGC 1 cut(s) 197
FspBI CTAG 1 cut(s) 279
GlaI GCGC 1 cut(s) 399
GluI GCNGC 1 cut(s) 197
HaeIII GGCC 1 cut(s) 30
HhaI GCGC 1 cut(s) 400
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HinfI GANTC 1 cut(s) 178
HphI GGTGA 2 cut(s) 55, 182
Hpy166II GTNNAC 1 cut(s) 173
Hpy188I TCNGA 2 cut(s) 115, 448
Hpy188III TCNNGA 2 cut(s) 78, 251
Hpy8I GTNNAC 1 cut(s) 173
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 20
HpyCH4III ACNGT 1 cut(s) 141
HpyCH4V TGCA 3 cut(s) 147, 440, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 470
HpyF3I CTNAG 3 cut(s) 20, 114, 258
HspAI GCGC 1 cut(s) 398
Kzo9I GATC 1 cut(s) 80
LmnI GCTCC 2 cut(s) 365, 433
LpnPI CCDG 2 cut(s) 254, 353
Lsp1109I GCAGC 1 cut(s) 208
LweI GCATC 3 cut(s) 94, 244, 449
MaeI CTAG 1 cut(s) 279
MalI GATC 1 cut(s) 82
MboI GATC 1 cut(s) 80
MboII GAAGA 2 cut(s) 221, 435
MluCI AATT 2 cut(s) 10, 273
MlyI GAGTC 1 cut(s) 172
MnlI CCTC 2 cut(s) 41, 441
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 2 cut(s) 92, 212
MwoI GCNNNNNNNGC 1 cut(s) 470
NdeII GATC 1 cut(s) 80
NlaIV GGNNCC 1 cut(s) 153
PceI AGGCCT 1 cut(s) 30
PdmI GAANNNNTTC 1 cut(s) 333
PkrI GCNGC 1 cut(s) 198
Ple19I CGATCG 1 cut(s) 83
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PspN4I GGNNCC 1 cut(s) 153
PvuI CGATCG 1 cut(s) 83
RsaI GTAC 1 cut(s) 456
RsaNI GTAC 1 cut(s) 455
SaqAI TTAA 2 cut(s) 92, 212
SatI GCNGC 1 cut(s) 197
Sau3AI GATC 1 cut(s) 80
SchI GAGTC 1 cut(s) 172
SetI ASST 4 cut(s) 39, 120, 201, 264
SfaNI GCATC 3 cut(s) 94, 244, 449
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
SpeI ACTAGT 1 cut(s) 278
Sse9I AATT 2 cut(s) 10, 273
SseBI AGGCCT 1 cut(s) 30
SspMI CTAG 1 cut(s) 279
StuI AGGCCT 1 cut(s) 30
TaaI ACNGT 1 cut(s) 141
TaqI TCGA 2 cut(s) 39, 159
TasI AATT 2 cut(s) 10, 273
Tru1I TTAA 2 cut(s) 92, 212
Tru9I TTAA 2 cut(s) 92, 212
TscAI CASTG 1 cut(s) 60
TseI GCWGC 1 cut(s) 196
TspDTI ATGAA 4 cut(s) 17, 91, 221, 235
TspGWI ACGGA 1 cut(s) 177
TspRI CASTG 1 cut(s) 60
XapI RAATTY 1 cut(s) 10
XmnI GAANNNNTTC 1 cut(s) 333
XspI CTAG 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.