Rmu_sc0000362.1_g000016

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000362.1
OG0020722
Physical Location & Seq
Reverse (-)
46520 .. 49236
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 819 bp
atgctcaccttagctctcatgttgccttcttattctggtacatatggaaggctagatgcaattgcgtttttaaacacccattctcgtgactcaaatggttccttcattggagttctgtcaaaaagtgattctgcttcctcttctcaagctgctgaaccctcagctcttgagcttcgtctcatcctagcctcttccatgtctctttcttcttttcagctggaatcagactgttttgctttgatttctacttttttaaatcctctgttggcggatgattggattgcttcgcaccttgtgttaacgattcgagctcgaggttctctcttcaacagtgtaaactggctttggaccagtagaaaagctaatgctgctgcagactacgcagcttctttggcttcgcacatggtgtacctgaatgattggatattttcatcattttactattcatcttatctgaaatcaaccagccaccactcgatcatctccatcctcttcttctcctccactgaaaccatcaccaacacctctcttagctatcaatgtttttcctccaccaactcgattcctttccaaccatgtgaatacattaatctttggaaagaactaccgaagctactagaccaagatggcgaagcggtgactcaacacgccggcgttgtagatttccgagaggctttggtctggttcttagccttgactgctactttgctcttcttcccccattgcggttgtctcactgcttctggagagaacccattaaccggctacgaggtgggtcgaattccggcgaggtgggtgcgaatattcctgccaatttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

272

Amino Acids

30.08

Weight (kDa)

5.58

Isoelectric Point (pI)

29.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 29 - 131 1e-07 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020722)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000362.1_g000016 Rmu_sc0006323.1_g000016
rosa_rugosa Rorug05G0547100
rosa_samantha Rh2BG003600 Rh2BG003700 Rh2BG003800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 269, 635, 726
AcsI RAATTY 1 cut(s) 780
AdeI CACNNNGTG 2 cut(s) 295, 406
AfaI GTAC 2 cut(s) 40, 410
AfiI CCNNNNNNNGG 2 cut(s) 725, 761
AgsI TTSAA 1 cut(s) 328
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 3 cut(s) 182, 204, 737
Ama87I CYCGRG 1 cut(s) 312
ApeKI GCWGC 4 cut(s) 149, 368, 371, 383
ApoI RAATTY 1 cut(s) 780
ArsI GACNNNNNNTTYG 2 cut(s) 688, 720
AseI ATTAAT 1 cut(s) 588
AspS9I GGNCC 1 cut(s) 348
AsuHPI GGTGA 2 cut(s) 508, 649
AvaI CYCGRG 1 cut(s) 312
AvaII GGWCC 1 cut(s) 348
BanII GRGCYC 1 cut(s) 313
BauI CACGAG 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 313
BbvCI CCTCAGC 1 cut(s) 160
BbvI GCAGC 4 cut(s) 136, 355, 358, 395
BccI CCATC 3 cut(s) 494, 521, 620
BcgI CGANNNNNNTGC 2 cut(s) 778, 812
BcoDI GTCTC 3 cut(s) 182, 204, 737
BfaI CTAG 3 cut(s) 53, 185, 617
BfmI CTRYAG 1 cut(s) 372
BisI GCNGC 4 cut(s) 150, 369, 372, 384
BlsI GCNGC 4 cut(s) 151, 370, 373, 385
Bme18I GGWCC 1 cut(s) 348
BmeT110I CYCGRG 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 1 cut(s) 100
BmsI GCATC 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 306, 338
BpmI CTGGAG 1 cut(s) 765
Bpu10I CCTNAGC 2 cut(s) 10, 160
BpuEI CTTGAG 2 cut(s) 129, 188
Bsc4I CCNNNNNNNGG 2 cut(s) 725, 761
Bse118I RCCGGY 2 cut(s) 650, 761
Bse1I ACTGG 2 cut(s) 344, 351
Bse3DI GCAATG 1 cut(s) 721
BseGI GGATG 3 cut(s) 180, 277, 486
BseLI CCNNNNNNNGG 2 cut(s) 725, 761
BseMI GCAATG 1 cut(s) 721
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 2 cut(s) 344, 351
BseRI GAGGAG 1 cut(s) 490
BseXI GCAGC 4 cut(s) 136, 355, 358, 395
BsiHKAI GWGCWC 1 cut(s) 313
BsiHKCI CYCGRG 1 cut(s) 312
BsiSI CCGG 3 cut(s) 651, 762, 785
BslI CCNNNNNNNGG 2 cut(s) 725, 761
BsmAI GTCTC 3 cut(s) 182, 204, 737
BsmBI CGTCTC 1 cut(s) 182
BsoBI CYCGRG 1 cut(s) 312
Bsp1286I GDGCHC 1 cut(s) 313
Bsp143I GATC 1 cut(s) 477
BspACI CCGC 3 cut(s) 269, 635, 726
BspCNI CTCAG 1 cut(s) 173
BspLI GGNNCC 1 cut(s) 100
BspMAI CTGCAG 1 cut(s) 376
BspQI GCTCTTC 1 cut(s) 716
BsrDI GCAATG 1 cut(s) 721
BsrFI RCCGGY 2 cut(s) 650, 761
BsrI ACTGG 2 cut(s) 344, 351
BssAI RCCGGY 2 cut(s) 650, 761
BssMI GATC 1 cut(s) 477
BssSI CACGAG 1 cut(s) 84
Bst2BI CACGAG 1 cut(s) 84
Bst4CI ACNGT 2 cut(s) 230, 332
Bst6I CTCTTC 5 cut(s) 145, 196, 329, 497, 716
BstC8I GCNNGC 1 cut(s) 652
BstDEI CTNAG 4 cut(s) 10, 160, 530, 688
BstF5I GGATG 3 cut(s) 180, 277, 486
BstKTI GATC 1 cut(s) 480
BstMAI GTCTC 3 cut(s) 182, 204, 737
BstMBI GATC 1 cut(s) 477
BstMWI GCNNNNNNNGC 4 cut(s) 368, 380, 392, 698
BstSFI CTRYAG 1 cut(s) 372
BstV1I GCAGC 4 cut(s) 136, 355, 358, 395
BtsCI GGATG 3 cut(s) 180, 277, 486
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 3 cut(s) 337, 504, 735
Cac8I GCNNGC 1 cut(s) 652
Cfr10I RCCGGY 2 cut(s) 650, 761
Cfr13I GGNCC 1 cut(s) 348
Csp6I GTAC 2 cut(s) 39, 409
CviAII CATG 4 cut(s) 19, 196, 403, 576
CviQI GTAC 2 cut(s) 39, 409
DdeI CTNAG 4 cut(s) 10, 160, 530, 688
DpnI GATC 1 cut(s) 479
DpnII GATC 1 cut(s) 477
DraI TTTAAA 2 cut(s) 72, 255
DraIII CACNNNGTG 2 cut(s) 295, 406
Eam1104I CTCTTC 5 cut(s) 145, 196, 329, 497, 716
EarI CTCTTC 5 cut(s) 145, 196, 329, 497, 716
EciI GGCGGA 1 cut(s) 284
Ecl136II GAGCTC 1 cut(s) 311
Eco24I GRGCYC 1 cut(s) 313
Eco47I GGWCC 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 311
Eco88I CYCGRG 1 cut(s) 312
EcoICRI GAGCTC 1 cut(s) 311
EcoRI GAATTC 1 cut(s) 780
EcoT38I GRGCYC 1 cut(s) 313
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 4 cut(s) 22, 199, 406, 579
FaiI YATR 6 cut(s) 20, 43, 45, 197, 404, 577
FatI CATG 4 cut(s) 18, 195, 402, 575
FauNDI CATATG 1 cut(s) 43
Fnu4HI GCNGC 4 cut(s) 150, 369, 372, 384
FokI GGATG 3 cut(s) 167, 284, 473
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 4 cut(s) 150, 369, 372, 384
FspBI CTAG 3 cut(s) 53, 185, 617
GluI GCNGC 4 cut(s) 150, 369, 372, 384
GsuI CTGGAG 1 cut(s) 765
HapII CCGG 3 cut(s) 651, 762, 785
Hin1II CATG 4 cut(s) 22, 199, 406, 579
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HinfI GANTC 6 cut(s) 89, 128, 221, 304, 562, 640
HpaI GTTAAC 1 cut(s) 300
HpaII CCGG 3 cut(s) 651, 762, 785
HphI GGTGA 2 cut(s) 508, 649
Hpy166II GTNNAC 3 cut(s) 300, 337, 409
Hpy188I TCNGA 3 cut(s) 226, 456, 668
Hpy188III TCNNGA 3 cut(s) 86, 167, 744
Hpy8I GTNNAC 3 cut(s) 300, 337, 409
HpyAV CCTTC 3 cut(s) 36, 42, 112
HpyCH4III ACNGT 2 cut(s) 230, 332
HpyCH4V TGCA 2 cut(s) 59, 374
HpyF10VI GCNNNNNNNGC 4 cut(s) 368, 380, 392, 698
HpyF3I CTNAG 4 cut(s) 10, 160, 530, 688
Hsp92II CATG 4 cut(s) 22, 199, 406, 579
KroI GCCGGC 1 cut(s) 650
KroNI GCCGGC 1 cut(s) 652
KspAI GTTAAC 1 cut(s) 300
Kzo9I GATC 1 cut(s) 477
LguI GCTCTTC 1 cut(s) 716
Lsp1109I GCAGC 4 cut(s) 136, 355, 358, 395
LweI GCATC 1 cut(s) 46
MaeI CTAG 3 cut(s) 53, 185, 617
MaeIII GTNAC 2 cut(s) 86, 637
MalI GATC 1 cut(s) 479
MboI GATC 1 cut(s) 477
MboII GAAGA 8 cut(s) 132, 183, 198, 316, 484, 487, 703, 706
MfeI CAATTG 1 cut(s) 60
MhlI GDGCHC 1 cut(s) 313
MluCI AATT 3 cut(s) 60, 780, 813
MlyI GAGTC 2 cut(s) 83, 634
MmeI TCCRAC 1 cut(s) 595
MreI CGCCGGCG 1 cut(s) 650
MroNI GCCGGC 1 cut(s) 650
MseI TTAA 5 cut(s) 71, 254, 299, 588, 758
MslI CAYNNNNRTG 1 cut(s) 84
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 3 cut(s) 651, 762, 785
MunI CAATTG 1 cut(s) 60
MwoI GCNNNNNNNGC 4 cut(s) 368, 380, 392, 698
NaeI GCCGGC 1 cut(s) 652
NdeI CATATG 1 cut(s) 43
NdeII GATC 1 cut(s) 477
NgoMIV GCCGGC 1 cut(s) 650
NlaIII CATG 4 cut(s) 22, 199, 406, 579
NlaIV GGNNCC 1 cut(s) 100
NmuCI GTSAC 2 cut(s) 86, 637
PaeR7I CTCGAG 1 cut(s) 312
PciSI GCTCTTC 1 cut(s) 716
PdiI GCCGGC 1 cut(s) 652
PfeI GAWTC 4 cut(s) 128, 221, 304, 562
PkrI GCNGC 4 cut(s) 151, 370, 373, 385
PleI GAGTC 2 cut(s) 83, 634
PpsI GAGTC 2 cut(s) 83, 634
PshBI ATTAAT 1 cut(s) 588
Psp124BI GAGCTC 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 100
PspPI GGNCC 1 cut(s) 348
PspXI VCTCGAGB 1 cut(s) 312
PstI CTGCAG 1 cut(s) 376
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 2 cut(s) 40, 410
RsaNI GTAC 2 cut(s) 39, 409
RseI CAYNNNNRTG 1 cut(s) 84
SacI GAGCTC 1 cut(s) 313
SapI GCTCTTC 1 cut(s) 716
SaqAI TTAA 5 cut(s) 71, 254, 299, 588, 758
SatI GCNGC 4 cut(s) 150, 369, 372, 384
Sau3AI GATC 1 cut(s) 477
Sau96I GGNCC 1 cut(s) 348
SchI GAGTC 2 cut(s) 83, 634
SduI GDGCHC 1 cut(s) 313
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 372
Sfr274I CTCGAG 1 cut(s) 312
SgrAI CRCCGGYG 1 cut(s) 650
SinI GGWCC 1 cut(s) 348
SlaI CTCGAG 1 cut(s) 312
SmiMI CAYNNNNRTG 1 cut(s) 84
SmlI CTYRAG 3 cut(s) 144, 167, 312
SmoI CTYRAG 3 cut(s) 144, 167, 312
Sse9I AATT 3 cut(s) 60, 780, 813
SsiI CCGC 3 cut(s) 269, 635, 726
SspI AATATT 1 cut(s) 804
SspMI CTAG 3 cut(s) 53, 185, 617
SstI GAGCTC 1 cut(s) 313
TaaI ACNGT 2 cut(s) 230, 332
TaqI TCGA 5 cut(s) 307, 313, 476, 560, 778
TasI AATT 3 cut(s) 60, 780, 813
TfiI GAWTC 4 cut(s) 128, 221, 304, 562
Tru1I TTAA 5 cut(s) 71, 254, 299, 588, 758
Tru9I TTAA 5 cut(s) 71, 254, 299, 588, 758
TscAI CASTG 3 cut(s) 337, 511, 742
TseFI GTSAC 2 cut(s) 86, 637
TseI GCWGC 4 cut(s) 149, 368, 371, 383
Tsp45I GTSAC 2 cut(s) 86, 637
TspDTI ATGAA 3 cut(s) 94, 420, 435
TspRI CASTG 3 cut(s) 337, 511, 742
VpaK11BI GGWCC 1 cut(s) 348
VspI ATTAAT 1 cut(s) 588
XapI RAATTY 1 cut(s) 780
XhoI CTCGAG 1 cut(s) 312
XspI CTAG 3 cut(s) 53, 185, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.