Rmu_sc0000542.1_g000014

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000542.1
OG0031079
Physical Location & Seq
Reverse (-)
71808 .. 74958
3151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000542.1_g000014.1.cds

Sequence Viewer

Length: 1263 bp
atgctgcctccacccatcgctgctgcctctcacctgcagatccctgcaacccagcaccgatcgcagcccgatcccatcgcagctgcctcccacatgcagatccttgcaacccagcaccgatcgcagcccgatcccaacactgcaacccagccccagcaccgatcgattcctgcaacccaccggacgtccgcgcctgctgcacttccagatcgatccggacaccagcccgcatcaccgcatcaatccaccgtcgcagcagctagattggactcgtccgctccccactgtcccagcacccacatcggcgacgacgtagcaagccccgcgcaccagacagccctgcccagtagcccactgccccgcgccaacagccaagcgccctccccgacacctgcagatcccagcccagcgcgctccagcctccgcgcacatcctcgatccctgcttggtgcgcccccagcagtacctgcaattctgcaaaccagaaggaaggaaagcaggaggaggaagaagaaaagtggaaaaaaagaggtcactcgggccgaaatggctgatccaactcgacctaaatttgacattttggacttagaagaacttgagtaccaccgttgggtttccaatgtagaaactgcctttgtggcaaaagactacactgccaccatccaaactcccactgaccccaaagacgatggactgtctaataagttgaagacacccaaagaactgtgggatgcccttaagggacgttttgggaacattcatgacaccttactcccaaaactgaccgttcaatggaatgaaatccgcttgcttgactacaaaaagagggaccttgggcttttcggcgacggcgaagttgagatcggagaggagatcgaagcagaggagcggctccggtggcttctgggcgtgaaagggtctgacgggtgttggtacgaagtgggtgtagaacgaggtcggagctggagtcattgtggagaacgagaagacgggctgggttctggaagcgaatcggttccgtcggcgatgatgacgtcatcggcgtcgaaagagagagatggtggtcgtggggtttggaattggattgaagttctgcatatgctattaagattgaactcttcgttgataattagattgaagttctgtttctttgtagtttttgctatggaaaatggatttgaattgtgttattatggaaatggtgaactgcaggagtcgatgggcaggcatgggagtggagaagaggaagaaggagaacagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

420

Amino Acids

46.25

Weight (kDa)

6.5

Isoelectric Point (pI)

65.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031079)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000542.1_g000014 Rmu_sc0001832.1_g000019

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 42, 400
AatII GACGTC 2 cut(s) 188, 1037
Acc36I ACCTGC 3 cut(s) 42, 400, 475
AccBSI CCGCTC 2 cut(s) 278, 889
AccII CGCG 5 cut(s) 191, 326, 363, 412, 426
AccIII TCCGGA 1 cut(s) 215
AciI CCGC 9 cut(s) 189, 228, 236, 276, 324, 361, 424, 805, 889
AclWI GGATC 8 cut(s) 34, 65, 94, 125, 207, 392, 432, 548
AcsI RAATTY 1 cut(s) 569
AcyI GRCGYC 3 cut(s) 185, 1034, 1043
AfaI GTAC 3 cut(s) 465, 602, 935
AfiI CCNNNNNNNGG 2 cut(s) 610, 792
AflII CTTAAG 1 cut(s) 737
AgsI TTSAA 6 cut(s) 709, 791, 1088, 1114, 1138, 1181
AluBI AGCT 3 cut(s) 83, 260, 963
AluI AGCT 3 cut(s) 83, 260, 963
AlwI GGATC 8 cut(s) 34, 65, 94, 125, 207, 392, 432, 548
AlwNI CAGNNNCTG 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 537
Aor13HI TCCGGA 1 cut(s) 215
AoxI GGCC 1 cut(s) 540
ApoI RAATTY 1 cut(s) 569
Asp700I GAANNNNTTC 1 cut(s) 1014
AspLEI GCGC 8 cut(s) 193, 328, 365, 379, 412, 414, 428, 454
AspS9I GGNCC 2 cut(s) 540, 829
AsuHPI GGTGA 3 cut(s) 23, 225, 1214
AvaI CYCGRG 1 cut(s) 537
AvaII GGWCC 1 cut(s) 829
BaeI ACNNNNGTAYC 2 cut(s) 584, 617
BbsI GAAGAC 2 cut(s) 716, 993
BbvI GCAGC 9 cut(s) 7, 10, 70, 76, 92, 136, 184, 266, 269
BccI CCATC 6 cut(s) 23, 83, 668, 683, 1052, 1213
BceAI ACGGC 1 cut(s) 865
BcgI CGANNNNNNTGC 2 cut(s) 283, 317
BfaI CTAG 1 cut(s) 261
BfmI CTRYAG 3 cut(s) 35, 393, 1208
BfoI RGCGCY 1 cut(s) 380
BfrI CTTAAG 1 cut(s) 737
BfuAI ACCTGC 3 cut(s) 42, 400, 475
BglI GCCNNNNNGGC 2 cut(s) 548, 638
Bme18I GGWCC 1 cut(s) 829
BmeT110I CYCGRG 1 cut(s) 537
BmgT120I GGNCC 2 cut(s) 540, 829
BmiI GGNNCC 3 cut(s) 830, 893, 1017
BmrI ACTGGG 1 cut(s) 339
BmsI GCATC 3 cut(s) 239, 247, 721
BmuI ACTGGG 1 cut(s) 339
BpiI GAAGAC 2 cut(s) 716, 993
BpmI CTGGAG 2 cut(s) 400, 985
BpuEI CTTGAG 1 cut(s) 617
Bsa29I ATCGAT 2 cut(s) 164, 211
BsaHI GRCGYC 3 cut(s) 185, 1034, 1043
BsaJI CCNNGG 1 cut(s) 832
BsaWI WCCGGW 3 cut(s) 180, 215, 894
BsaXI ACNNNNNCTCC 4 cut(s) 952, 958, 982, 988
Bsc4I CCNNNNNNNGG 2 cut(s) 610, 792
Bse1I ACTGG 1 cut(s) 345
BseAI TCCGGA 1 cut(s) 215
BseCI ATCGAT 2 cut(s) 164, 211
BseDI CCNNGG 1 cut(s) 832
BseGI GGATG 3 cut(s) 430, 660, 736
BseLI CCNNNNNNNGG 2 cut(s) 610, 792
BseNI ACTGG 1 cut(s) 345
BsePI GCGCGC 1 cut(s) 410
BseRI GAGGAG 3 cut(s) 517, 884, 899
BseXI GCAGC 9 cut(s) 7, 10, 70, 76, 92, 136, 184, 266, 269
BseYI CCCAGC 9 cut(s) 51, 111, 147, 153, 290, 401, 406, 457, 994
BsgI GTGCAG 1 cut(s) 183
Bsh1236I CGCG 5 cut(s) 191, 326, 363, 412, 426
Bsh1285I CGRYCG 3 cut(s) 62, 122, 164
BshFI GGCC 1 cut(s) 542
BshVI ATCGAT 2 cut(s) 164, 211
BsiEI CGRYCG 3 cut(s) 62, 122, 164
BsiHKCI CYCGRG 1 cut(s) 537
BsiSI CCGG 3 cut(s) 181, 216, 895
BslFI GGGAC 3 cut(s) 273, 756, 842
BslI CCNNNNNNNGG 2 cut(s) 610, 792
BsmFI GGGAC 3 cut(s) 273, 756, 842
BsnI GGCC 1 cut(s) 542
BsoBI CYCGRG 1 cut(s) 537
Bsp13I TCCGGA 1 cut(s) 215
BspACI CCGC 9 cut(s) 189, 228, 236, 276, 324, 361, 424, 805, 889
BspANI GGCC 1 cut(s) 542
BspDI ATCGAT 2 cut(s) 164, 211
BspEI TCCGGA 1 cut(s) 215
BspFNI CGCG 5 cut(s) 191, 326, 363, 412, 426
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 3 cut(s) 830, 893, 1017
BspMAI CTGCAG 3 cut(s) 39, 397, 1212
BspMI ACCTGC 3 cut(s) 42, 400, 475
BspPI GGATC 8 cut(s) 34, 65, 94, 125, 207, 392, 432, 548
BspTI CTTAAG 1 cut(s) 737
BsrBI CCGCTC 2 cut(s) 278, 889
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 832
BssHII GCGCGC 1 cut(s) 410
BssNI GRCGYC 3 cut(s) 185, 1034, 1043
BssT1I CCWWGG 1 cut(s) 832
Bst4CI ACNGT 7 cut(s) 250, 287, 608, 696, 726, 787, 1260
Bst6I CTCTTC 2 cut(s) 1123, 1236
BstACI GRCGYC 3 cut(s) 185, 1034, 1043
BstAFI CTTAAG 1 cut(s) 737
BstAPI GCANNNNNTGC 1 cut(s) 467
BstC8I GCNNGC 6 cut(s) 195, 228, 319, 412, 809, 1226
BstDEI CTNAG 1 cut(s) 586
BstF5I GGATG 3 cut(s) 430, 660, 736
BstFNI CGCG 5 cut(s) 191, 326, 363, 412, 426
BstH2I RGCGCY 1 cut(s) 380
BstHHI GCGC 8 cut(s) 193, 328, 365, 379, 412, 414, 428, 454
BstMCI CGRYCG 3 cut(s) 62, 122, 164
BstNSI RCATGY 1 cut(s) 97
BstSFI CTRYAG 3 cut(s) 35, 393, 1208
BstUI CGCG 5 cut(s) 191, 326, 363, 412, 426
BstV1I GCAGC 9 cut(s) 7, 10, 70, 76, 92, 136, 184, 266, 269
BstV2I GAAGAC 2 cut(s) 716, 993
BstX2I RGATCY 3 cut(s) 39, 99, 397
BstYI RGATCY 3 cut(s) 39, 99, 397
Bsu15I ATCGAT 2 cut(s) 164, 211
BsuRI GGCC 1 cut(s) 542
BsuTUI ATCGAT 2 cut(s) 164, 211
BtgZI GCGATG 2 cut(s) 61, 1040
BtsCI GGATG 3 cut(s) 430, 660, 736
BtsI GCAGTG 3 cut(s) 138, 353, 651
BtsIMutI CAGTG 5 cut(s) 138, 283, 353, 651, 672
BveI ACCTGC 3 cut(s) 42, 400, 475
Cac8I GCNNGC 6 cut(s) 195, 228, 319, 412, 809, 1226
CaiI CAGNNNCTG 1 cut(s) 467
CciI TCATGA 1 cut(s) 760
CfoI GCGC 8 cut(s) 193, 328, 365, 379, 412, 414, 428, 454
Cfr13I GGNCC 2 cut(s) 540, 829
ClaI ATCGAT 2 cut(s) 164, 211
CseI GACGC 1 cut(s) 1032
Csp6I GTAC 3 cut(s) 464, 601, 934
CviAII CATG 3 cut(s) 94, 761, 1229
CviQI GTAC 3 cut(s) 464, 601, 934
DdeI CTNAG 1 cut(s) 586
Eam1104I CTCTTC 2 cut(s) 1123, 1236
EarI CTCTTC 2 cut(s) 1123, 1236
Eco130I CCWWGG 1 cut(s) 832
Eco47I GGWCC 1 cut(s) 829
Eco88I CYCGRG 1 cut(s) 537
EcoO109I RGGNCCY 1 cut(s) 829
EcoT14I CCWWGG 1 cut(s) 832
ErhI CCWWGG 1 cut(s) 832
FaeI CATG 3 cut(s) 97, 764, 1232
FaiI YATR 7 cut(s) 95, 762, 1098, 1100, 1166, 1194, 1230
FaqI GGGAC 3 cut(s) 273, 756, 842
FatI CATG 3 cut(s) 93, 760, 1228
FauI CCCGC 3 cut(s) 235, 331, 368
FauNDI CATATG 1 cut(s) 1098
FokI GGATG 3 cut(s) 417, 647, 743
FspBI CTAG 1 cut(s) 261
GlaI GCGC 8 cut(s) 192, 327, 364, 378, 411, 413, 427, 453
GsaI CCCAGC 9 cut(s) 55, 115, 151, 157, 294, 405, 410, 461, 998
GsuI CTGGAG 2 cut(s) 400, 985
HaeII RGCGCY 1 cut(s) 380
HaeIII GGCC 1 cut(s) 542
HapII CCGG 3 cut(s) 181, 216, 895
HgaI GACGC 1 cut(s) 1032
HhaI GCGC 8 cut(s) 193, 328, 365, 379, 412, 414, 428, 454
Hin1I GRCGYC 3 cut(s) 185, 1034, 1043
Hin1II CATG 3 cut(s) 97, 764, 1232
Hin6I GCGC 8 cut(s) 191, 326, 363, 377, 410, 412, 426, 452
HinP1I GCGC 8 cut(s) 191, 326, 363, 377, 410, 412, 426, 452
HinfI GANTC 5 cut(s) 166, 269, 967, 1010, 1214
HpaII CCGG 3 cut(s) 181, 216, 895
HphI GGTGA 3 cut(s) 23, 225, 1214
Hpy166II GTNNAC 1 cut(s) 1205
Hpy188I TCNGA 3 cut(s) 866, 922, 960
Hpy188III TCNNGA 4 cut(s) 206, 216, 761, 1002
Hpy8I GTNNAC 1 cut(s) 1205
Hpy99I CGWCG 6 cut(s) 254, 311, 314, 851, 1024, 1048
HpyAV CCTTC 3 cut(s) 480, 484, 1244
HpyCH4III ACNGT 7 cut(s) 250, 287, 608, 696, 726, 787, 1260
HpyCH4IV ACGT 4 cut(s) 185, 312, 745, 1034
HpyF3I CTNAG 1 cut(s) 586
HpySE526I ACGT 4 cut(s) 185, 312, 745, 1034
Hsp92I GRCGYC 3 cut(s) 185, 1034, 1043
Hsp92II CATG 3 cut(s) 97, 764, 1232
HspAI GCGC 8 cut(s) 191, 326, 363, 377, 410, 412, 426, 452
Kpn2I TCCGGA 1 cut(s) 215
LmnI GCTCC 5 cut(s) 283, 419, 886, 897, 960
Lsp1109I GCAGC 9 cut(s) 7, 10, 70, 76, 92, 136, 184, 266, 269
LweI GCATC 3 cut(s) 239, 247, 721
MaeI CTAG 1 cut(s) 261
MaeII ACGT 4 cut(s) 185, 312, 745, 1034
MaeIII GTNAC 1 cut(s) 532
MbiI CCGCTC 2 cut(s) 278, 889
MboII GAAGA 8 cut(s) 520, 523, 602, 721, 998, 1110, 1253, 1259
MflI RGATCY 3 cut(s) 39, 99, 397
MluCI AATT 5 cut(s) 471, 569, 1078, 1128, 1181
MlyI GAGTC 3 cut(s) 263, 976, 1223
MmeI TCCRAC 2 cut(s) 581, 938
MroI TCCGGA 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 1014
MseI TTAA 2 cut(s) 738, 1106
MslI CAYNNNNRTG 1 cut(s) 1233
MspA1I CMGCKG 1 cut(s) 83
MspCI CTTAAG 1 cut(s) 737
MspI CCGG 3 cut(s) 181, 216, 895
MvnI CGCG 5 cut(s) 191, 326, 363, 412, 426
NdeI CATATG 1 cut(s) 1098
NlaIII CATG 3 cut(s) 97, 764, 1232
NlaIV GGNNCC 3 cut(s) 830, 893, 1017
NmuCI GTSAC 1 cut(s) 532
NspI RCATGY 1 cut(s) 97
PagI TCATGA 1 cut(s) 760
PaqCI CACCTGC 2 cut(s) 42, 400
PauI GCGCGC 1 cut(s) 410
PcsI WCGNNNNNNNCGW 3 cut(s) 309, 849, 1040
PdmI GAANNNNTTC 1 cut(s) 1014
PfeI GAWTC 2 cut(s) 166, 1010
Ple19I CGATCG 3 cut(s) 62, 122, 164
PleI GAGTC 3 cut(s) 263, 975, 1222
PpsI GAGTC 3 cut(s) 263, 975, 1222
PpuMI RGGWCCY 1 cut(s) 829
Psp5II RGGWCCY 1 cut(s) 829
PspFI CCCAGC 9 cut(s) 51, 111, 147, 153, 290, 401, 406, 457, 994
PspN4I GGNNCC 3 cut(s) 830, 893, 1017
PspPI GGNCC 2 cut(s) 540, 829
PspPPI RGGWCCY 1 cut(s) 829
PstI CTGCAG 3 cut(s) 39, 397, 1212
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 3 cut(s) 39, 99, 397
PteI GCGCGC 1 cut(s) 410
PvuI CGATCG 3 cut(s) 62, 122, 164
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 3 cut(s) 465, 602, 935
RsaNI GTAC 3 cut(s) 464, 601, 934
RseI CAYNNNNRTG 1 cut(s) 1233
SaqAI TTAA 2 cut(s) 738, 1106
Sau96I GGNCC 2 cut(s) 540, 829
SchI GAGTC 3 cut(s) 263, 976, 1223
SfaNI GCATC 3 cut(s) 239, 247, 721
SfcI CTRYAG 3 cut(s) 35, 393, 1208
SinI GGWCC 1 cut(s) 829
SmiMI CAYNNNNRTG 1 cut(s) 1233
SmlI CTYRAG 2 cut(s) 596, 737
SmoI CTYRAG 2 cut(s) 596, 737
Sse9I AATT 5 cut(s) 471, 569, 1078, 1128, 1181
SsiI CCGC 9 cut(s) 189, 228, 236, 276, 324, 361, 424, 805, 889
SspMI CTAG 1 cut(s) 261
StyI CCWWGG 1 cut(s) 832
TaaI ACNGT 7 cut(s) 250, 287, 608, 696, 726, 787, 1260
TaiI ACGT 4 cut(s) 188, 315, 748, 1037
TaqI TCGA 7 cut(s) 164, 211, 436, 562, 876, 1046, 1217
TasI AATT 5 cut(s) 471, 569, 1078, 1128, 1181
TauI GCSGC 1 cut(s) 892
TfiI GAWTC 2 cut(s) 166, 1010
Tru1I TTAA 2 cut(s) 738, 1106
Tru9I TTAA 2 cut(s) 738, 1106
TscAI CASTG 5 cut(s) 145, 290, 360, 658, 679
TseFI GTSAC 1 cut(s) 532
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 2 cut(s) 749, 813
TspGWI ACGGA 1 cut(s) 1008
TspRI CASTG 5 cut(s) 145, 290, 360, 658, 679
Vha464I CTTAAG 1 cut(s) 737
VpaK11BI GGWCC 1 cut(s) 829
XapI RAATTY 1 cut(s) 569
XceI RCATGY 1 cut(s) 97
XmnI GAANNNNTTC 1 cut(s) 1014
XspI CTAG 1 cut(s) 261
ZraI GACGTC 2 cut(s) 186, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.