Rmu_sc0000830.1_g000053

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000830.1
OG0025494
Physical Location & Seq
Reverse (-)
218673 .. 220557
1885 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 735 bp
atggagaacgaggctgaggttgacgagtccgggctgcagagggacgaggctgaggtcgacgggtccaggctgcagagggaccaaatggatgagattgagtcgatctttgggtgggtgcccaaagtaaatcgcaaatcgctgggtctggtgaaacgaaggcgatctggtctaggcggagaacgagaactgatgttagcgattgggattgaggagctcgacgacggagcagagcaaatagcagtcgatattgggtggccggacgggaactgggttgagtctgggtacgaagcagattggcgacaaactgaagagattgatctcttctctggtgtcggaggcagacaaaggcagctggataacgaggcaggggttataaccttggaagaactagcgtcggtgatccaatcactagatgaacgtccgacgaaagaagaagttcatgaaatgattagtgagattagtgctgatggaaatggaacaatagattttgaagagttcttgaatatcatggcaaggaagatgcaggaaaacgttgctgaggagctaaaagaagcattcaaagtatttgacagggatcaagacggttatatttcagcttctgagttgagacaggtgatgataaatttgggagaaagattgagcaatgaagaggctgagcaaatgatcagagaggctgatttggatggtgacggtctagttagctatgaagaatttgcaaggatgatgctcagctag

Protein Analysis

244

Amino Acids

27.61

Weight (kDa)

4.19

Isoelectric Point (pI)

47.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 121 - 170 1.7e-08 EF-hand domain pair
EF-hand_8 PF13833 123 - 172 2.3e-07 EF-hand domain pair
EF-hand_7 PF13499 180 - 242 5.1e-19 EF-hand domain pair
EF-hand_1 PF00036 181 - 208 6.9e-09 EF hand domain
EF-hand_6 PF13405 181 - 210 3.7e-09 EF-hand domain
EF-hand_5 PF13202 182 - 206 7.7e-08 EF hand
EF-hand_8 PF13833 202 - 242 5.3e-07 EF-hand domain pair
EF-hand_1 PF00036 217 - 242 2.6e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025494)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8302471.1_g000001 Rmu_sc0000830.1_g000053
rosa_samantha Rh2DG426300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 374
AccB1I GGYRCC 1 cut(s) 115
AccI GTMKAC 1 cut(s) 57
AciI CCGC 1 cut(s) 174
AclI AACGTT 1 cut(s) 531
AclWI GGATC 2 cut(s) 394, 582
AcoI YGGCCR 1 cut(s) 254
AcsI RAATTY 2 cut(s) 622, 710
AcuI CTGAAG 1 cut(s) 327
AfaI GTAC 1 cut(s) 284
AgsI TTSAA 3 cut(s) 491, 502, 559
AjnI CCWGG 1 cut(s) 65
AluBI AGCT 6 cut(s) 214, 352, 544, 596, 702, 732
AluI AGCT 6 cut(s) 214, 352, 544, 596, 702, 732
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 1 cut(s) 601
AlwI GGATC 2 cut(s) 394, 582
AlwNI CAGNNNCTG 1 cut(s) 599
AoxI GGCC 1 cut(s) 254
ApeKI GCWGC 3 cut(s) 34, 70, 349
ApoI RAATTY 2 cut(s) 622, 710
Asp700I GAANNNNTTC 1 cut(s) 435
AspS9I GGNCC 2 cut(s) 63, 79
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 4 cut(s) 160, 409, 625, 698
AvaII GGWCC 2 cut(s) 63, 79
BaeGI GKGCMC 1 cut(s) 120
BanI GGYRCC 1 cut(s) 115
BanII GRGCYC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 216
BbvCI CCTCAGC 3 cut(s) 15, 51, 537
BbvI GCAGC 3 cut(s) 21, 57, 361
BccI CCATC 2 cut(s) 461, 677
BciT130I CCWGG 1 cut(s) 67
BclI TGATCA 1 cut(s) 663
BcnI CCSGG 1 cut(s) 31
BcoDI GTCTC 1 cut(s) 601
BfaI CTAG 5 cut(s) 170, 389, 410, 695, 733
BfmI CTRYAG 2 cut(s) 35, 71
BisI GCNGC 3 cut(s) 35, 71, 350
BlpI GCTNAGC 2 cut(s) 654, 728
BlsI GCNGC 3 cut(s) 36, 72, 351
Bme1390I CCNGG 2 cut(s) 31, 67
Bme18I GGWCC 2 cut(s) 63, 79
BmgT120I GGNCC 2 cut(s) 63, 79
BmiI GGNNCC 3 cut(s) 64, 80, 117
BmrFI CCNGG 2 cut(s) 31, 67
BmrI ACTGGG 1 cut(s) 277
BmsI GCATC 2 cut(s) 510, 714
BmuI ACTGGG 1 cut(s) 277
Bpu10I CCTNAGC 3 cut(s) 15, 51, 537
Bpu1102I GCTNAGC 2 cut(s) 654, 728
BpuMI CCSGG 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 378
Bse1I ACTGG 1 cut(s) 272
Bse3DI GCAATG 1 cut(s) 649
BseBI CCWGG 1 cut(s) 67
BseDI CCNNGG 1 cut(s) 378
BseGI GGATG 3 cut(s) 94, 688, 726
BseMI GCAATG 1 cut(s) 649
BseMII CTCAG 5 cut(s) 6, 42, 528, 591, 645
BseNI ACTGG 1 cut(s) 272
BseRI GAGGAG 2 cut(s) 224, 554
BseSI GKGCMC 1 cut(s) 120
BseXI GCAGC 3 cut(s) 21, 57, 361
BseYI CCCAGC 1 cut(s) 139
BshFI GGCC 1 cut(s) 256
BshNI GGYRCC 1 cut(s) 115
BsiHKAI GWGCWC 1 cut(s) 216
BsiSI CCGG 2 cut(s) 30, 257
BslFI GGGAC 2 cut(s) 56, 92
BsmAI GTCTC 1 cut(s) 601
BsmFI GGGAC 2 cut(s) 56, 92
BsmI GAATGC 1 cut(s) 554
BsnI GGCC 1 cut(s) 256
Bsp1286I GDGCHC 2 cut(s) 120, 216
Bsp143I GATC 6 cut(s) 102, 161, 316, 399, 574, 663
Bsp1720I GCTNAGC 2 cut(s) 654, 728
BspACI CCGC 1 cut(s) 174
BspANI GGCC 1 cut(s) 256
BspCNI CTCAG 5 cut(s) 7, 43, 529, 592, 646
BspHI TCATGA 1 cut(s) 439
BspLI GGNNCC 3 cut(s) 64, 80, 117
BspMAI CTGCAG 2 cut(s) 39, 75
BspPI GGATC 2 cut(s) 394, 582
BspT107I GGYRCC 1 cut(s) 115
BsrDI GCAATG 1 cut(s) 649
BsrI ACTGG 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 378
BssMI GATC 6 cut(s) 102, 161, 316, 399, 574, 663
BssT1I CCWWGG 1 cut(s) 378
Bst2UI CCWGG 1 cut(s) 67
Bst4CI ACNGT 2 cut(s) 584, 692
Bst6I CTCTTC 4 cut(s) 303, 326, 486, 642
BstDEI CTNAG 6 cut(s) 15, 51, 537, 600, 654, 728
BstF5I GGATG 3 cut(s) 94, 688, 726
BstKTI GATC 6 cut(s) 105, 164, 319, 402, 577, 666
BstMAI GTCTC 1 cut(s) 601
BstMBI GATC 6 cut(s) 102, 161, 316, 399, 574, 663
BstNI CCWGG 1 cut(s) 67
BstSCI CCNGG 2 cut(s) 29, 65
BstSFI CTRYAG 2 cut(s) 35, 71
BstSLI GKGCMC 1 cut(s) 120
BstV1I GCAGC 3 cut(s) 21, 57, 361
BsuRI GGCC 1 cut(s) 256
BtsCI GGATG 3 cut(s) 94, 688, 726
CaiI CAGNNNCTG 1 cut(s) 599
CciI TCATGA 1 cut(s) 439
Cfr13I GGNCC 2 cut(s) 63, 79
CseI GACGC 1 cut(s) 381
Csp6I GTAC 1 cut(s) 283
CviAII CATG 2 cut(s) 440, 508
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 6 cut(s) 15, 51, 537, 600, 654, 728
DpnI GATC 6 cut(s) 104, 163, 318, 401, 576, 665
DpnII GATC 6 cut(s) 102, 161, 316, 399, 574, 663
EaeI YGGCCR 1 cut(s) 254
Eam1104I CTCTTC 4 cut(s) 303, 326, 486, 642
EarI CTCTTC 4 cut(s) 303, 326, 486, 642
EciI GGCGGA 1 cut(s) 189
Ecl136II GAGCTC 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 378
Eco24I GRGCYC 1 cut(s) 216
Eco47I GGWCC 2 cut(s) 63, 79
Eco53kI GAGCTC 1 cut(s) 214
Eco57I CTGAAG 1 cut(s) 327
EcoICRI GAGCTC 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 2 cut(s) 443, 511
FaiI YATR 5 cut(s) 374, 441, 509, 588, 705
FaqI GGGAC 2 cut(s) 56, 92
FatI CATG 2 cut(s) 439, 507
FbaI TGATCA 1 cut(s) 663
FblI GTMKAC 1 cut(s) 57
Fnu4HI GCNGC 3 cut(s) 35, 71, 350
FokI GGATG 2 cut(s) 101, 695
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 3 cut(s) 35, 71, 350
FspBI CTAG 5 cut(s) 170, 389, 410, 695, 733
GluI GCNGC 3 cut(s) 35, 71, 350
GsaI CCCAGC 1 cut(s) 143
HaeIII GGCC 1 cut(s) 256
HapII CCGG 2 cut(s) 30, 257
HgaI GACGC 1 cut(s) 381
Hin1II CATG 2 cut(s) 443, 511
HincII GTYRAC 2 cut(s) 22, 58
HindII GTYRAC 2 cut(s) 22, 58
HinfI GANTC 3 cut(s) 26, 98, 275
HpaII CCGG 2 cut(s) 30, 257
HphI GGTGA 4 cut(s) 160, 409, 625, 698
Hpy166II GTNNAC 2 cut(s) 22, 58
Hpy188I TCNGA 4 cut(s) 335, 423, 601, 668
Hpy188III TCNNGA 3 cut(s) 440, 499, 578
Hpy8I GTNNAC 2 cut(s) 22, 58
Hpy99I CGWCG 5 cut(s) 62, 221, 224, 397, 427
HpyAV CCTTC 1 cut(s) 150
HpyCH4III ACNGT 2 cut(s) 584, 692
HpyCH4IV ACGT 2 cut(s) 418, 531
HpyCH4V TGCA 4 cut(s) 37, 73, 523, 716
HpyF3I CTNAG 6 cut(s) 15, 51, 537, 600, 654, 728
HpySE526I ACGT 2 cut(s) 418, 531
Hsp92II CATG 2 cut(s) 443, 511
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 6 cut(s) 102, 161, 316, 399, 574, 663
LmnI GCTCC 3 cut(s) 211, 224, 541
Lsp1109I GCAGC 3 cut(s) 21, 57, 361
LweI GCATC 2 cut(s) 510, 714
MaeI CTAG 5 cut(s) 170, 389, 410, 695, 733
MaeII ACGT 2 cut(s) 418, 531
MaeIII GTNAC 1 cut(s) 686
MalI GATC 6 cut(s) 104, 163, 318, 401, 576, 665
MboI GATC 6 cut(s) 102, 161, 316, 399, 574, 663
MboII GAAGA 8 cut(s) 313, 320, 395, 443, 503, 529, 659, 719
MhlI GDGCHC 2 cut(s) 120, 216
MluCI AATT 2 cut(s) 622, 710
MlyI GAGTC 3 cut(s) 35, 107, 284
MmeI TCCRAC 2 cut(s) 313, 446
MroXI GAANNNNTTC 1 cut(s) 435
MspA1I CMGCKG 1 cut(s) 352
MspI CCGG 2 cut(s) 30, 257
MspR9I CCNGG 2 cut(s) 31, 67
Mva1269I GAATGC 1 cut(s) 554
MvaI CCWGG 1 cut(s) 67
NciI CCSGG 1 cut(s) 31
NdeII GATC 6 cut(s) 102, 161, 316, 399, 574, 663
NlaIII CATG 2 cut(s) 443, 511
NlaIV GGNNCC 3 cut(s) 64, 80, 117
NmuCI GTSAC 1 cut(s) 686
PagI TCATGA 1 cut(s) 439
PctI GAATGC 1 cut(s) 554
PdmI GAANNNNTTC 1 cut(s) 435
PkrI GCNGC 3 cut(s) 36, 72, 351
PleI GAGTC 3 cut(s) 34, 106, 283
PpsI GAGTC 3 cut(s) 34, 106, 283
PsiI TTATAA 1 cut(s) 374
Psp124BI GAGCTC 1 cut(s) 216
Psp1406I AACGTT 1 cut(s) 531
Psp6I CCWGG 1 cut(s) 65
PspFI CCCAGC 1 cut(s) 139
PspGI CCWGG 1 cut(s) 65
PspN4I GGNNCC 3 cut(s) 64, 80, 117
PspPI GGNCC 2 cut(s) 63, 79
PstI CTGCAG 2 cut(s) 39, 75
PstNI CAGNNNCTG 1 cut(s) 599
PvuII CAGCTG 1 cut(s) 352
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
SacI GAGCTC 1 cut(s) 216
SalI GTCGAC 1 cut(s) 56
SatI GCNGC 3 cut(s) 35, 71, 350
Sau3AI GATC 6 cut(s) 102, 161, 316, 399, 574, 663
Sau96I GGNCC 2 cut(s) 63, 79
SchI GAGTC 3 cut(s) 35, 107, 284
ScrFI CCNGG 2 cut(s) 31, 67
SduI GDGCHC 2 cut(s) 120, 216
SfaNI GCATC 2 cut(s) 510, 714
SfcI CTRYAG 2 cut(s) 35, 71
SinI GGWCC 2 cut(s) 63, 79
Sse9I AATT 2 cut(s) 622, 710
SsiI CCGC 1 cut(s) 174
SspMI CTAG 5 cut(s) 170, 389, 410, 695, 733
SstI GAGCTC 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 29, 65
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 2 cut(s) 584, 692
TaiI ACGT 2 cut(s) 421, 534
TaqI TCGA 4 cut(s) 57, 101, 216, 243
TasI AATT 2 cut(s) 622, 710
TseFI GTSAC 1 cut(s) 686
TseI GCWGC 3 cut(s) 34, 70, 349
Tsp45I GTSAC 1 cut(s) 686
TspDTI ATGAA 5 cut(s) 428, 429, 456, 660, 720
TspGWI ACGGA 1 cut(s) 237
VpaK11BI GGWCC 2 cut(s) 63, 79
XapI RAATTY 2 cut(s) 622, 710
XmiI GTMKAC 1 cut(s) 57
XmnI GAANNNNTTC 1 cut(s) 435
XspI CTAG 5 cut(s) 170, 389, 410, 695, 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.