Rmu_sc0001009.1_g000030

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001009.1
OG0031112
Physical Location & Seq
Forward (+)
159243 .. 161218
1976 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 885 bp
atgcctttgcaaattgcagctaatagttctgatcaaacgaaggatcaggatctgctatctcacccgttgaagagcctagccaatcttgctggtacagttgatgggagaaacatatctacattacttcaggcatctcaaagtttacctaacactgggtcttgcatcaagaccgcacaacaggttctagatacggtttccaacatctttgaacccagtaggctttctgtttctgcctcaagtatggatgattgtgttattattgaggaacctttaaggcccataggacagtgccgatattacctgcatcagatatgcaaaagcgaagatcttcagttgatggcgatcttggaaaatcgcatagaccgcattgtttttaaactctttggaaaagacccgaatgaacttccatttgttctgcgatcacagattatcgactggttatcccatagtcctacagagattgaaagctatataaggccaagatgcatcattttgacaatttacctccgtctagaaaaatccacgtgggaggagctctgctatcatctgggatccagtttgcaaagacttttagatgccaccaatgatcctttctggagaacaggatgggtgtatactaggatgcaacattttgttgcgtttatgtacaatgaagggaagtatctggcgcaagaagcttgtgatgacgtgatggatggtgctgatacaactgttgagcatgatgagctacagtgtattagattctcctgctctataccaaatgttactaggcgaggattcattgagtgggctaggagagccaaccatgtttatggtcaagtggaaattgatgaagtgcgtaatgagatcgcaagttatgtggtgaagaacatcctcaagatgtaa

Protein Analysis

294

Amino Acids

33.67

Weight (kDa)

5.32

Isoelectric Point (pI)

53.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_SPL7 PF26102 215 - 262 1.9e-09 SPL7-like, immunoglobulin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0031112)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001009.1_g000030 Rmu_sc0007272.1_g000009

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 309
AccI GTMKAC 1 cut(s) 614
AciI CCGC 2 cut(s) 171, 364
AclWI GGATC 5 cut(s) 51, 57, 546, 559, 581
AcuI CTGAAG 2 cut(s) 110, 314
AcvI CACGTG 1 cut(s) 525
AfaI GTAC 2 cut(s) 94, 647
AfiI CCNNNNNNNGG 1 cut(s) 152
AgsI TTSAA 3 cut(s) 70, 209, 464
AjiI CACGTC 1 cut(s) 688
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
AluBI AGCT 5 cut(s) 20, 468, 535, 677, 727
AluI AGCT 5 cut(s) 20, 468, 535, 677, 727
Alw21I GWGCWC 1 cut(s) 537
AlwI GGATC 5 cut(s) 51, 57, 546, 559, 581
AlwNI CAGNNNCTG 1 cut(s) 52
AoxI GGCC 2 cut(s) 275, 476
ApeKI GCWGC 1 cut(s) 17
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 1 cut(s) 276
AsuHPI GGTGA 2 cut(s) 53, 874
BaeI ACNNNNGTAYC 2 cut(s) 696, 729
BamHI GGATCC 1 cut(s) 551
BanII GRGCYC 1 cut(s) 537
BbrPI CACGTG 1 cut(s) 525
Bbv12I GWGCWC 1 cut(s) 537
BbvI GCAGC 1 cut(s) 29
BccI CCATC 5 cut(s) 95, 331, 600, 685, 689
BclI TGATCA 1 cut(s) 31
BfaI CTAG 6 cut(s) 77, 185, 512, 618, 768, 792
BfmI CTRYAG 2 cut(s) 453, 728
BfuAI ACCTGC 1 cut(s) 309
BglII AGATCT 1 cut(s) 325
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
BmgBI CACGTC 1 cut(s) 688
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 2 cut(s) 267, 553
BmrI ACTGGG 2 cut(s) 162, 207
BmsI GCATC 7 cut(s) 140, 171, 313, 473, 495, 565, 612
BmuI ACTGGG 2 cut(s) 162, 207
BpmI CTGGAG 1 cut(s) 616
BpuEI CTTGAG 2 cut(s) 220, 860
BsaAI YACGTR 1 cut(s) 525
BsaBI GATNNNNATC 2 cut(s) 48, 341
Bsc4I CCNNNNNNNGG 1 cut(s) 152
Bse1I ACTGG 4 cut(s) 157, 213, 440, 555
Bse8I GATNNNNATC 2 cut(s) 48, 341
BseGI GGATG 5 cut(s) 250, 611, 627, 700, 870
BseJI GATNNNNATC 2 cut(s) 48, 341
BseLI CCNNNNNNNGG 1 cut(s) 152
BseNI ACTGG 4 cut(s) 157, 213, 440, 555
BseRI GAGGAG 1 cut(s) 545
BseXI GCAGC 1 cut(s) 29
BshFI GGCC 2 cut(s) 277, 478
BsiHKAI GWGCWC 1 cut(s) 537
BslI CCNNNNNNNGG 1 cut(s) 152
BsnI GGCC 2 cut(s) 277, 478
Bsp1286I GDGCHC 1 cut(s) 537
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 9 cut(s) 31, 43, 49, 325, 342, 419, 551, 586, 846
BspACI CCGC 2 cut(s) 171, 364
BspANI GGCC 2 cut(s) 277, 478
BspLI GGNNCC 2 cut(s) 267, 553
BspMI ACCTGC 1 cut(s) 309
BspPI GGATC 5 cut(s) 51, 57, 546, 559, 581
BspQI GCTCTTC 1 cut(s) 65
BsrGI TGTACA 1 cut(s) 645
BsrI ACTGG 4 cut(s) 157, 213, 440, 555
BssMI GATC 9 cut(s) 31, 43, 49, 325, 342, 419, 551, 586, 846
BssNAI GTATAC 1 cut(s) 615
Bst1107I GTATAC 1 cut(s) 615
Bst4CI ACNGT 5 cut(s) 97, 193, 288, 712, 732
Bst6I CTCTTC 1 cut(s) 65
BstAUI TGTACA 1 cut(s) 645
BstBAI YACGTR 1 cut(s) 525
BstF5I GGATG 5 cut(s) 250, 611, 627, 700, 870
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 9 cut(s) 34, 46, 52, 328, 345, 422, 554, 589, 849
BstMBI GATC 9 cut(s) 31, 43, 49, 325, 342, 419, 551, 586, 846
BstMWI GCNNNNNNNGC 5 cut(s) 86, 363, 674, 724, 797
BstSFI CTRYAG 2 cut(s) 453, 728
BstV1I GCAGC 1 cut(s) 29
BstX2I RGATCY 3 cut(s) 49, 325, 551
BstXI CCANNNNNNTGG 1 cut(s) 812
BstYI RGATCY 3 cut(s) 49, 325, 551
BstZ17I GTATAC 1 cut(s) 615
BsuRI GGCC 2 cut(s) 277, 478
BtrI CACGTC 1 cut(s) 688
BtsCI GGATG 5 cut(s) 250, 611, 627, 700, 870
BtsIMutI CAGTG 3 cut(s) 150, 293, 737
BveI ACCTGC 1 cut(s) 309
CaiI CAGNNNCTG 1 cut(s) 52
CfoI GCGC 1 cut(s) 670
Cfr13I GGNCC 1 cut(s) 276
Csp6I GTAC 2 cut(s) 93, 646
CspCI CAANNNNNGTGG 2 cut(s) 840, 875
CviAII CATG 2 cut(s) 719, 806
CviQI GTAC 2 cut(s) 93, 646
DpnI GATC 9 cut(s) 33, 45, 51, 327, 344, 421, 553, 588, 848
DpnII GATC 9 cut(s) 31, 43, 49, 325, 342, 419, 551, 586, 846
DraI TTTAAA 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Ecl136II GAGCTC 1 cut(s) 535
Eco24I GRGCYC 1 cut(s) 537
Eco53kI GAGCTC 1 cut(s) 535
Eco57I CTGAAG 2 cut(s) 110, 314
Eco72I CACGTG 1 cut(s) 525
EcoICRI GAGCTC 1 cut(s) 535
EcoT22I ATGCAT 1 cut(s) 488
EcoT38I GRGCYC 1 cut(s) 537
FaeI CATG 2 cut(s) 722, 809
FatI CATG 2 cut(s) 718, 805
FbaI TGATCA 1 cut(s) 31
FblI GTMKAC 1 cut(s) 614
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 5 cut(s) 257, 618, 634, 707, 857
FriOI GRGCYC 1 cut(s) 537
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 6 cut(s) 77, 185, 512, 618, 768, 792
GlaI GCGC 1 cut(s) 669
GluI GCNGC 1 cut(s) 18
GsuI CTGGAG 1 cut(s) 616
HaeIII GGCC 2 cut(s) 277, 478
HhaI GCGC 1 cut(s) 670
Hin1II CATG 2 cut(s) 722, 809
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 2 cut(s) 741, 777
HphI GGTGA 2 cut(s) 53, 874
Hpy166II GTNNAC 2 cut(s) 143, 615
Hpy188I TCNGA 2 cut(s) 31, 309
Hpy188III TCNNGA 6 cut(s) 47, 166, 185, 512, 595, 877
Hpy8I GTNNAC 2 cut(s) 143, 615
HpyAV CCTTC 2 cut(s) 34, 647
HpyCH4III ACNGT 5 cut(s) 97, 193, 288, 712, 732
HpyCH4IV ACGT 2 cut(s) 524, 687
HpyCH4V TGCA 8 cut(s) 10, 17, 162, 304, 315, 486, 562, 625
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 363, 674, 724, 797
HpySE526I ACGT 2 cut(s) 524, 687
Hsp92II CATG 2 cut(s) 722, 809
HspAI GCGC 1 cut(s) 668
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 9 cut(s) 31, 43, 49, 325, 342, 419, 551, 586, 846
LguI GCTCTTC 1 cut(s) 65
LmnI GCTCC 1 cut(s) 532
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 7 cut(s) 140, 171, 313, 473, 495, 565, 612
MaeI CTAG 6 cut(s) 77, 185, 512, 618, 768, 792
MaeII ACGT 2 cut(s) 524, 687
MaeIII GTNAC 1 cut(s) 763
MalI GATC 9 cut(s) 33, 45, 51, 327, 344, 421, 553, 588, 848
MboI GATC 9 cut(s) 31, 43, 49, 325, 342, 419, 551, 586, 846
MboII GAAGA 4 cut(s) 82, 320, 335, 877
MflI RGATCY 3 cut(s) 49, 325, 551
MhlI GDGCHC 1 cut(s) 537
MluCI AATT 3 cut(s) 12, 498, 825
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 6 cut(s) 244, 256, 515, 523, 767, 884
Mph1103I ATGCAT 1 cut(s) 488
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 2 cut(s) 272, 375
MslI CAYNNNNRTG 1 cut(s) 810
MwoI GCNNNNNNNGC 5 cut(s) 86, 363, 674, 724, 797
NdeII GATC 9 cut(s) 31, 43, 49, 325, 342, 419, 551, 586, 846
NlaIII CATG 2 cut(s) 722, 809
NlaIV GGNNCC 2 cut(s) 267, 553
NsiI ATGCAT 1 cut(s) 488
PciSI GCTCTTC 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 2 cut(s) 741, 777
PkrI GCNGC 1 cut(s) 19
PmaCI CACGTG 1 cut(s) 525
PmlI CACGTG 1 cut(s) 525
Ppu21I YACGTR 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 537
PspCI CACGTG 1 cut(s) 525
PspN4I GGNNCC 2 cut(s) 267, 553
PspPI GGNCC 1 cut(s) 276
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 3 cut(s) 49, 325, 551
RsaI GTAC 2 cut(s) 94, 647
RsaNI GTAC 2 cut(s) 93, 646
RseI CAYNNNNRTG 1 cut(s) 810
SacI GAGCTC 1 cut(s) 537
SapI GCTCTTC 1 cut(s) 65
SaqAI TTAA 2 cut(s) 272, 375
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 9 cut(s) 31, 43, 49, 325, 342, 419, 551, 586, 846
Sau96I GGNCC 1 cut(s) 276
SduI GDGCHC 1 cut(s) 537
SfaNI GCATC 7 cut(s) 140, 171, 313, 473, 495, 565, 612
SfcI CTRYAG 2 cut(s) 453, 728
SmiMI CAYNNNNRTG 1 cut(s) 810
SmlI CTYRAG 2 cut(s) 235, 875
SmoI CTYRAG 2 cut(s) 235, 875
Sse9I AATT 3 cut(s) 12, 498, 825
SsiI CCGC 2 cut(s) 171, 364
SspMI CTAG 6 cut(s) 77, 185, 512, 618, 768, 792
SstI GAGCTC 1 cut(s) 537
TaaI ACNGT 5 cut(s) 97, 193, 288, 712, 732
TaiI ACGT 2 cut(s) 527, 690
TaqI TCGA 1 cut(s) 432
TasI AATT 3 cut(s) 12, 498, 825
TatI WGTACW 1 cut(s) 645
TfiI GAWTC 2 cut(s) 741, 777
Tru1I TTAA 2 cut(s) 272, 375
Tru9I TTAA 2 cut(s) 272, 375
TscAI CASTG 3 cut(s) 157, 293, 737
TseI GCWGC 1 cut(s) 17
TspDTI ATGAA 4 cut(s) 414, 666, 769, 846
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 3 cut(s) 157, 293, 737
XbaI TCTAGA 2 cut(s) 184, 511
XmiI GTMKAC 1 cut(s) 614
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 6 cut(s) 77, 185, 512, 618, 768, 792
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.