Rmu_sc0001035.1_g000009

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001035.1
OG0022656
Physical Location & Seq
Reverse (-)
48702 .. 52191
3490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001035.1_g000009.1.cds

Sequence Viewer

Length: 810 bp
ctggatctccgactctctcctcttcctctcaactccaccgaccacatctccgtctcaggctccttgacgactccctccctccacggttccgtcctccaagtctacctccaaatcgaacagttgttgatcgacgccgcagtctccaagttcttctccgacgttctccctcagaacctcaacgtcgaagttgcagagtttgaagctgccattttgaagtcgaatcggagctatatcgaagcagtgtgcttcaagttcttggacatgcgtaatgaacggcaaataatgcagcttgtactcaaggtgaccaaagaaccaggacagcttgcgagagtttccttggacacacatggcactcgtgaggtgcagaagttgatagagactctggaaaatgggaaacaggtctctttgaccatatcatcccttgaatgcgtagcttttgtgactttgacggaggttgctggaaacctcaccagagtagcttttgtgactttgacggaggttgttggaaatctcaccagagtagcttttgttattaattttgtgacttcgccggaggttgtcagaaatctcaccatagtggcttttgttgctagtgacagtagcttttgtgacatctccggaggttgtcggaaatctcaccaaagtaggttttgtgttgctagaagttggccggagattcgttggaattgtagctttgtggtccccggagttgaccggagacccgccggagttcgccggaagttggtcggaggtcggccggagacctgccggaagttggccggaggtcgaccggagacccgccggagttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

269

Amino Acids

29.73

Weight (kDa)

9.06

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022656)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0161571
rosa_multiflora Rmu_sc0001035.1_g000009
rosa_samantha Rh2BG570300 Rh7AG104300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 773
AccI GTMKAC 2 cut(s) 102, 787
AccIII TCCGGA 1 cut(s) 617
AciI CCGC 3 cut(s) 135, 723, 799
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 3 cut(s) 668, 755, 777
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 1 cut(s) 294
AfiI CCNNNNNNNGG 2 cut(s) 742, 775
AgsI TTSAA 4 cut(s) 200, 214, 250, 425
AjnI CCWGG 1 cut(s) 313
AleI CACNNNNGTG 1 cut(s) 575
AluBI AGCT 9 cut(s) 203, 228, 289, 322, 434, 479, 524, 603, 693
AluI AGCT 9 cut(s) 203, 228, 289, 322, 434, 479, 524, 603, 693
Alw26I GTCTC 7 cut(s) 58, 145, 371, 406, 712, 755, 788
AlwI GGATC 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 617
AoxI GGCC 3 cut(s) 668, 755, 777
ApeKI GCWGC 2 cut(s) 203, 286
AseI ATTAAT 1 cut(s) 534
AspS9I GGNCC 1 cut(s) 700
AsuC2I CCSGG 1 cut(s) 705
AsuHPI GGTGA 5 cut(s) 313, 460, 505, 562, 629
AvaII GGWCC 1 cut(s) 700
BauI CACGAG 1 cut(s) 354
BbvI GCAGC 2 cut(s) 190, 298
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 1 cut(s) 315
BcnI CCSGG 1 cut(s) 705
BcoDI GTCTC 7 cut(s) 58, 145, 371, 406, 712, 755, 788
BfaI CTAG 2 cut(s) 591, 660
BfuAI ACCTGC 1 cut(s) 773
BisI GCNGC 3 cut(s) 135, 204, 287
BlsI GCNGC 3 cut(s) 136, 205, 288
Bme1390I CCNGG 2 cut(s) 315, 705
Bme18I GGWCC 1 cut(s) 700
BmgT120I GGNCC 1 cut(s) 700
BmiI GGNNCC 3 cut(s) 61, 88, 702
BmrFI CCNGG 2 cut(s) 315, 705
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 705
BsaHI GRCGYC 1 cut(s) 132
BsaI GGTCTC 4 cut(s) 406, 712, 755, 788
BsaJI CCNNGG 3 cut(s) 82, 336, 703
BsaWI WCCGGW 3 cut(s) 617, 714, 790
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 2 cut(s) 742, 775
BseAI TCCGGA 1 cut(s) 617
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 3 cut(s) 82, 336, 703
BseGI GGATG 1 cut(s) 416
BseLI CCNNNNNNNGG 2 cut(s) 742, 775
BseMII CTCAG 2 cut(s) 69, 182
BseRI GAGGAG 1 cut(s) 9
BseX3I CGGCCG 1 cut(s) 755
BseXI GCAGC 2 cut(s) 190, 298
BsgI GTGCAG 1 cut(s) 383
Bsh1285I CGRYCG 2 cut(s) 758, 791
BshFI GGCC 3 cut(s) 670, 757, 779
BsiEI CGRYCG 2 cut(s) 758, 791
BslFI GGGAC 1 cut(s) 686
BslI CCNNNNNNNGG 2 cut(s) 742, 775
BsmAI GTCTC 7 cut(s) 58, 145, 371, 406, 712, 755, 788
BsmBI CGTCTC 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 686
BsmI GAATGC 1 cut(s) 431
BsnI GGCC 3 cut(s) 670, 757, 779
Bso31I GGTCTC 4 cut(s) 406, 712, 755, 788
Bsp13I TCCGGA 1 cut(s) 617
Bsp143I GATC 2 cut(s) 4, 126
BspACI CCGC 3 cut(s) 135, 723, 799
BspANI GGCC 3 cut(s) 670, 757, 779
BspCNI CTCAG 2 cut(s) 68, 181
BspEI TCCGGA 1 cut(s) 617
BspLI GGNNCC 3 cut(s) 61, 88, 702
BspMI ACCTGC 1 cut(s) 773
BspPI GGATC 1 cut(s) 12
BspTNI GGTCTC 4 cut(s) 406, 712, 755, 788
BssECI CCNNGG 3 cut(s) 82, 336, 703
BssMI GATC 2 cut(s) 4, 126
BssNI GRCGYC 1 cut(s) 132
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 1 cut(s) 336
Bst2BI CACGAG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 3 cut(s) 86, 120, 599
Bst6I CTCTTC 1 cut(s) 27
BstACI GRCGYC 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 283
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 2 cut(s) 55, 168
BstDSI CCRYGG 1 cut(s) 82
BstEII GGTNACC 1 cut(s) 301
BstF5I GGATG 1 cut(s) 416
BstKTI GATC 2 cut(s) 7, 129
BstMAI GTCTC 7 cut(s) 58, 145, 371, 406, 712, 755, 788
BstMBI GATC 2 cut(s) 4, 126
BstMCI CGRYCG 2 cut(s) 758, 791
BstMWI GCNNNNNNNGC 2 cut(s) 283, 587
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 1 cut(s) 265
BstPI GGTNACC 1 cut(s) 301
BstSCI CCNGG 2 cut(s) 313, 703
BstV1I GCAGC 2 cut(s) 190, 298
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZI CGGCCG 1 cut(s) 755
BsuRI GGCC 3 cut(s) 670, 757, 779
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 1 cut(s) 416
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 1 cut(s) 246
BveI ACCTGC 1 cut(s) 773
Cac8I GCNNGC 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 700
CseI GACGC 1 cut(s) 140
Csp6I GTAC 1 cut(s) 293
CviAII CATG 2 cut(s) 262, 347
CviQI GTAC 1 cut(s) 293
DdeI CTNAG 2 cut(s) 55, 168
DpnI GATC 2 cut(s) 6, 128
DpnII GATC 2 cut(s) 4, 126
DrdI GACNNNNNNGTC 1 cut(s) 137
DseDI GACNNNNNNGTC 1 cut(s) 137
EaeI YGGCCR 3 cut(s) 668, 755, 777
EagI CGGCCG 1 cut(s) 755
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
EclXI CGGCCG 1 cut(s) 755
Eco130I CCWWGG 1 cut(s) 336
Eco31I GGTCTC 4 cut(s) 406, 712, 755, 788
Eco47I GGWCC 1 cut(s) 700
Eco52I CGGCCG 1 cut(s) 755
Eco91I GGTNACC 1 cut(s) 301
EcoO65I GGTNACC 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 336
Esp3I CGTCTC 1 cut(s) 58
FaeI CATG 2 cut(s) 265, 350
FaiI YATR 5 cut(s) 231, 263, 348, 413, 575
FaqI GGGAC 1 cut(s) 686
FatI CATG 2 cut(s) 261, 346
FauI CCCGC 2 cut(s) 730, 806
FblI GTMKAC 2 cut(s) 102, 787
Fnu4HI GCNGC 3 cut(s) 135, 204, 287
FokI GGATG 1 cut(s) 403
Fsp4HI GCNGC 3 cut(s) 135, 204, 287
FspBI CTAG 2 cut(s) 591, 660
GluI GCNGC 3 cut(s) 135, 204, 287
HaeIII GGCC 3 cut(s) 670, 757, 779
HgaI GACGC 1 cut(s) 140
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 2 cut(s) 265, 350
HincII GTYRAC 2 cut(s) 712, 788
HindII GTYRAC 2 cut(s) 712, 788
HinfI GANTC 5 cut(s) 12, 70, 220, 379, 676
HphI GGTGA 5 cut(s) 313, 460, 505, 562, 629
Hpy166II GTNNAC 3 cut(s) 103, 712, 788
Hpy188I TCNGA 7 cut(s) 11, 157, 171, 225, 563, 630, 749
Hpy188III TCNNGA 3 cut(s) 356, 383, 618
Hpy8I GTNNAC 3 cut(s) 103, 712, 788
Hpy99I CGWCG 3 cut(s) 134, 161, 185
HpyCH4III ACNGT 3 cut(s) 86, 120, 599
HpyCH4IV ACGT 2 cut(s) 159, 180
HpyCH4V TGCA 3 cut(s) 191, 286, 364
HpyF10VI GCNNNNNNNGC 2 cut(s) 283, 587
HpyF3I CTNAG 2 cut(s) 55, 168
HpySE526I ACGT 2 cut(s) 159, 180
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 2 cut(s) 265, 350
Kpn2I TCCGGA 1 cut(s) 617
Kzo9I GATC 2 cut(s) 4, 126
LmnI GCTCC 2 cut(s) 65, 225
Lsp1109I GCAGC 2 cut(s) 190, 298
MaeI CTAG 2 cut(s) 591, 660
MaeII ACGT 2 cut(s) 159, 180
MaeIII GTNAC 6 cut(s) 301, 439, 484, 541, 593, 608
MalI GATC 2 cut(s) 6, 128
MboI GATC 2 cut(s) 4, 126
MboII GAAGA 2 cut(s) 14, 142
MflI RGATCY 1 cut(s) 4
MluCI AATT 2 cut(s) 535, 685
MlyI GAGTC 3 cut(s) 6, 64, 373
MmeI TCCRAC 6 cut(s) 34, 180, 484, 608, 662, 727
MroI TCCGGA 1 cut(s) 617
MseI TTAA 1 cut(s) 534
MslI CAYNNNNRTG 1 cut(s) 575
MspR9I CCNGG 2 cut(s) 315, 705
Mva1269I GAATGC 1 cut(s) 431
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 2 cut(s) 283, 587
NciI CCSGG 1 cut(s) 705
NdeII GATC 2 cut(s) 4, 126
NlaIII CATG 2 cut(s) 265, 350
NlaIV GGNNCC 3 cut(s) 61, 88, 702
NmuCI GTSAC 6 cut(s) 301, 439, 484, 541, 593, 608
NspI RCATGY 1 cut(s) 265
OliI CACNNNNGTG 1 cut(s) 575
PctI GAATGC 1 cut(s) 431
PfeI GAWTC 2 cut(s) 220, 676
PkrI GCNGC 3 cut(s) 136, 205, 288
PleI GAGTC 3 cut(s) 6, 64, 373
PpsI GAGTC 3 cut(s) 6, 64, 373
PshBI ATTAAT 1 cut(s) 534
Psp6I CCWGG 1 cut(s) 313
PspEI GGTNACC 1 cut(s) 301
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 61, 88, 702
PspPI GGNCC 1 cut(s) 700
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 294
RsaNI GTAC 1 cut(s) 293
RseI CAYNNNNRTG 1 cut(s) 575
SalI GTCGAC 1 cut(s) 786
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 3 cut(s) 135, 204, 287
Sau3AI GATC 2 cut(s) 4, 126
Sau96I GGNCC 1 cut(s) 700
SchI GAGTC 3 cut(s) 6, 64, 373
ScrFI CCNGG 2 cut(s) 315, 705
SinI GGWCC 1 cut(s) 700
SmiMI CAYNNNNRTG 1 cut(s) 575
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 2 cut(s) 535, 685
SsiI CCGC 3 cut(s) 135, 723, 799
SspMI CTAG 2 cut(s) 591, 660
StyD4I CCNGG 2 cut(s) 313, 703
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 3 cut(s) 86, 120, 599
TaiI ACGT 2 cut(s) 162, 183
TaqI TCGA 6 cut(s) 114, 129, 183, 218, 234, 787
TasI AATT 2 cut(s) 535, 685
TatI WGTACW 1 cut(s) 292
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 2 cut(s) 220, 676
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 6 cut(s) 301, 439, 484, 541, 593, 608
TseI GCWGC 2 cut(s) 203, 286
Tsp45I GTSAC 6 cut(s) 301, 439, 484, 541, 593, 608
TspDTI ATGAA 1 cut(s) 285
TspGWI ACGGA 4 cut(s) 40, 79, 464, 509
TspRI CASTG 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 700
VspI ATTAAT 1 cut(s) 534
XceI RCATGY 1 cut(s) 265
XmiI GTMKAC 2 cut(s) 102, 787
XspI CTAG 2 cut(s) 591, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.