Rmu_sc0001089.1_g000019

coenzyme F420-1:gamma-L-glutamate ligase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001089.1
OG0025467
Physical Location & Seq
Reverse (-)
78487 .. 79062
576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001089.1_g000019.1.cds

Sequence Viewer

Length: 576 bp
atgcaaggaatactgccatcgctccctcccacaaggttcttgttccctctaacctctctcctccacctccatccccaccaccacaaccatgtccttctcttcctcctctccttccctttcttcactctccacccccaaacaacccgacccgcaactcgaccaaaacccctcgcacgtcctcacgtaccacaacgacaccaagcaccacttcacttgctacgccagaggccctcgcagactagactgggccaaccagcccaacccattctgccgctacctttcctccctctcctccaccccacaccacccaacgatcacccctcccctctctactccctcttcacctctctccctcctccaaaacccatctccaaatccaccatttcccaactcctttacgactctctagctctctccgcttggaaaaccaccggcttctccacctggtctctcagagtcaatcccagcaacggtaatctacaccccaccgaggcttatataatctccccgccgatcccttccctctccgattccggcttcgtggcccatccattaaagtcgagcgctttgcgctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nitroreductase

Protein Analysis

191

Amino Acids

21.31

Weight (kDa)

11.47

Isoelectric Point (pI)

56.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025467)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001089.1_g000019 Rmu_sc0002131.1_g000002 Rmu_ssc0000456.1_g000022

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 150, 272, 417, 509
AclWI GGATC 1 cut(s) 508
AfaI GTAC 1 cut(s) 186
AfeI AGCGCT 1 cut(s) 565
AfiI CCNNNNNNNGG 2 cut(s) 470, 490
AjiI CACGTC 1 cut(s) 176
AjnI CCWGG 1 cut(s) 443
AjuI GAANNNNNNNTTGG 2 cut(s) 192, 224
AluBI AGCT 1 cut(s) 410
AluI AGCT 1 cut(s) 410
Alw26I GTCTC 1 cut(s) 453
AlwI GGATC 1 cut(s) 508
Aor51HI AGCGCT 1 cut(s) 565
AoxI GGCC 3 cut(s) 227, 247, 543
AspLEI GCGC 2 cut(s) 566, 573
AspS9I GGNCC 3 cut(s) 228, 247, 544
AsuHPI GGTGA 2 cut(s) 308, 334
BccI CCATC 4 cut(s) 25, 78, 374, 555
BcgI CGANNNNNNTGC 1 cut(s) 550
BciT130I CCWGG 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 453
BfaI CTAG 3 cut(s) 240, 407, 574
BfoI RGCGCY 1 cut(s) 567
BisI GCNGC 1 cut(s) 272
BlsI GCNGC 1 cut(s) 273
Bme1390I CCNGG 1 cut(s) 445
BmgBI CACGTC 1 cut(s) 176
BmgT120I GGNCC 3 cut(s) 228, 247, 544
BmrFI CCNGG 1 cut(s) 445
BmrI ACTGGG 1 cut(s) 254
BmuI ACTGGG 1 cut(s) 254
BsaAI YACGTR 1 cut(s) 184
BsaI GGTCTC 1 cut(s) 453
BsaJI CCNNGG 1 cut(s) 489
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 2 cut(s) 470, 490
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 1 cut(s) 249
BseBI CCWGG 1 cut(s) 445
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 2 cut(s) 70, 547
BseLI CCNNNNNNNGG 2 cut(s) 470, 490
BseMII CTCAG 1 cut(s) 466
BseNI ACTGG 1 cut(s) 249
BseRI GAGGAG 4 cut(s) 50, 95, 281, 345
BseYI CCCAGC 1 cut(s) 464
BshFI GGCC 3 cut(s) 229, 249, 545
BsiSI CCGG 2 cut(s) 432, 534
BslI CCNNNNNNNGG 2 cut(s) 470, 490
BsmAI GTCTC 1 cut(s) 453
BsnI GGCC 3 cut(s) 229, 249, 545
Bso31I GGTCTC 1 cut(s) 453
Bsp143I GATC 2 cut(s) 313, 513
BspACI CCGC 4 cut(s) 150, 272, 417, 509
BspANI GGCC 3 cut(s) 229, 249, 545
BspCNI CTCAG 1 cut(s) 465
BspPI GGATC 1 cut(s) 508
BspTNI GGTCTC 1 cut(s) 453
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 1 cut(s) 249
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 2 cut(s) 313, 513
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 1 cut(s) 473
Bst6I CTCTTC 2 cut(s) 104, 344
BstBAI YACGTR 1 cut(s) 184
BstDEI CTNAG 1 cut(s) 452
BstF5I GGATG 2 cut(s) 70, 547
BstH2I RGCGCY 1 cut(s) 567
BstHHI GCGC 2 cut(s) 566, 573
BstKTI GATC 2 cut(s) 316, 516
BstMAI GTCTC 1 cut(s) 453
BstMBI GATC 2 cut(s) 313, 513
BstMWI GCNNNNNNNGC 2 cut(s) 416, 570
BstNI CCWGG 1 cut(s) 445
BstSCI CCNGG 1 cut(s) 443
BsuRI GGCC 3 cut(s) 229, 249, 545
BtgZI GCGATG 1 cut(s) 3
BtrI CACGTC 1 cut(s) 176
BtsCI GGATG 2 cut(s) 70, 547
CfoI GCGC 2 cut(s) 566, 573
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 3 cut(s) 228, 247, 544
CsiI ACCWGGT 1 cut(s) 443
Csp6I GTAC 1 cut(s) 185
CspCI CAANNNNNGTGG 2 cut(s) 195, 230
CviAII CATG 1 cut(s) 89
CviJI RGCY 8 cut(s) 229, 249, 257, 410, 435, 494, 537, 545
CviKI_1 RGCY 8 cut(s) 229, 249, 257, 410, 435, 494, 537, 545
CviQI GTAC 1 cut(s) 185
DdeI CTNAG 1 cut(s) 452
DpnI GATC 2 cut(s) 315, 515
DpnII GATC 2 cut(s) 313, 513
Eam1104I CTCTTC 2 cut(s) 104, 344
EarI CTCTTC 2 cut(s) 104, 344
Eco31I GGTCTC 1 cut(s) 453
Eco47III AGCGCT 1 cut(s) 565
EcoO109I RGGNCCY 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 443
FaeI CATG 1 cut(s) 92
FaiI YATR 3 cut(s) 90, 498, 500
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FatI CATG 1 cut(s) 88
FauI CCCGC 2 cut(s) 157, 516
Fnu4HI GCNGC 1 cut(s) 272
FokI GGATG 2 cut(s) 57, 534
Fsp4HI GCNGC 1 cut(s) 272
FspBI CTAG 3 cut(s) 240, 407, 574
GlaI GCGC 2 cut(s) 565, 572
GluI GCNGC 1 cut(s) 272
GsaI CCCAGC 1 cut(s) 468
HaeII RGCGCY 1 cut(s) 567
HaeIII GGCC 3 cut(s) 229, 249, 545
HapII CCGG 2 cut(s) 432, 534
HhaI GCGC 2 cut(s) 566, 573
Hin1II CATG 1 cut(s) 92
Hin6I GCGC 2 cut(s) 564, 571
HinP1I GCGC 2 cut(s) 564, 571
HinfI GANTC 3 cut(s) 401, 456, 530
HpaII CCGG 2 cut(s) 432, 534
HphI GGTGA 2 cut(s) 308, 334
Hpy188I TCNGA 2 cut(s) 455, 529
HpyAV CCTTC 3 cut(s) 104, 121, 528
HpyCH4III ACNGT 1 cut(s) 473
HpyCH4IV ACGT 2 cut(s) 175, 183
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 2 cut(s) 416, 570
HpyF3I CTNAG 1 cut(s) 452
HpySE526I ACGT 2 cut(s) 175, 183
Hsp92II CATG 1 cut(s) 92
HspAI GCGC 2 cut(s) 564, 571
Kzo9I GATC 2 cut(s) 313, 513
LmnI GCTCC 1 cut(s) 27
LpnPI CCDG 8 cut(s) 230, 236, 267, 430, 445, 457, 478, 547
MabI ACCWGGT 1 cut(s) 443
MaeI CTAG 3 cut(s) 240, 407, 574
MaeII ACGT 2 cut(s) 175, 183
MalI GATC 2 cut(s) 315, 515
MboI GATC 2 cut(s) 313, 513
MboII GAAGA 3 cut(s) 91, 112, 331
MlyI GAGTC 2 cut(s) 395, 465
MseI TTAA 1 cut(s) 554
MslI CAYNNNNRTG 1 cut(s) 87
MspI CCGG 2 cut(s) 432, 534
MspR9I CCNGG 1 cut(s) 445
MvaI CCWGG 1 cut(s) 445
MwoI GCNNNNNNNGC 2 cut(s) 416, 570
NdeII GATC 2 cut(s) 313, 513
NlaIII CATG 1 cut(s) 92
PfeI GAWTC 1 cut(s) 530
PkrI GCNGC 1 cut(s) 273
PleI GAGTC 2 cut(s) 395, 464
PpsI GAGTC 2 cut(s) 395, 464
Ppu21I YACGTR 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 443
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 1 cut(s) 443
PspPI GGNCC 3 cut(s) 228, 247, 544
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
RseI CAYNNNNRTG 1 cut(s) 87
SaqAI TTAA 1 cut(s) 554
SatI GCNGC 1 cut(s) 272
Sau3AI GATC 2 cut(s) 313, 513
Sau96I GGNCC 3 cut(s) 228, 247, 544
SchI GAGTC 2 cut(s) 395, 465
ScrFI CCNGG 1 cut(s) 445
SetI ASST 9 cut(s) 38, 56, 69, 178, 186, 280, 347, 412, 446
SexAI ACCWGGT 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 87
SsiI CCGC 4 cut(s) 150, 272, 417, 509
SspMI CTAG 3 cut(s) 240, 407, 574
StyD4I CCNGG 1 cut(s) 443
TaaI ACNGT 1 cut(s) 473
TaiI ACGT 2 cut(s) 178, 186
TaqI TCGA 2 cut(s) 157, 560
TauI GCSGC 1 cut(s) 274
TfiI GAWTC 1 cut(s) 530
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
XspI CTAG 3 cut(s) 240, 407, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.