Rmu_sc0001148.1_g000012

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001148.1
OG0030945
Physical Location & Seq
Forward (+)
55111 .. 56719
1609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001148.1_g000012.1.cds

Sequence Viewer

Length: 963 bp
atggagagccaagcaaacattcatctcagtatcttattagaagaagatgcttggaatttgtttgtgaagaaagcaagaaagtcttttgaaaaatccactaatttctatgatgtagcgagaaaggttgttagagaatgtgctggtctcccaattgcgttgatagcagttgcgagggcacttggagataaagattttgaagaatggaaagaagcagctcgacgccttgagatgtcacaacgtgccaaccttgatcagaaagatgtgttcaactctataaagttaagctatgattacttggacggtaacgatgccaagtcttgcttcttgctttgctgcctattcccagaagattatgacattcaattggaagacttgatgatatatgggtttggaaaaggattgtttcgagatgttaacagcacactagaaaatgccagagtcgtagcaaattcggatggatgcgtaaggatgcatgatgtcattcgggatgtggctatgtcaattgccttgtctgtagatggcctcgcctctttggtaaaaactcgctgtgatttaaaagattggccaacgacggatgcgcacgatggctactcagcaatctcactaatggaaagcaacatttgcaagcttcccaacaagttgagggcccaggatcccatcccgcaaattgactttccacatcatcattcaagagcattgactcttaacacaatgatgaaaacctttggctcagttaaatcaaattcggctgctactggcttcatatttcgagaccattgtggaaaacggatcattgcaactgcaaagaatatgccaatatgggagtcaccacagttcctgttgcggaagcaactgctctcagaaatgcccttatcactgcgcatgagaaaggatttaccaaagttgaagtggaaggagattccaattaagttagtcattgacgcagttaatggagttgtttga

Protein Analysis

320

Amino Acids

36.24

Weight (kDa)

8.05

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030945)

Species Orthologous Gene IDs
rosa_laevigata RLG00000036829
rosa_multiflora Rmu_sc0001148.1_g000012

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 579, 881
AciI CCGC 2 cut(s) 662, 844
AclWI GGATC 3 cut(s) 647, 660, 797
AcoI YGGCCR 1 cut(s) 563
AcsI RAATTY 3 cut(s) 55, 448, 742
AcyI GRCGYC 1 cut(s) 220
AdeI CACNNNGTG 1 cut(s) 239
AgsI TTSAA 6 cut(s) 89, 197, 268, 362, 690, 907
AjnI CCWGG 1 cut(s) 648
AjuI GAANNNNNNNTTGG 3 cut(s) 35, 305, 337
AluBI AGCT 3 cut(s) 215, 285, 628
AluI AGCT 3 cut(s) 215, 285, 628
Alw26I GTCTC 2 cut(s) 149, 765
AlwI GGATC 3 cut(s) 647, 660, 797
AlwNI CAGNNNCTG 1 cut(s) 838
AoxI GGCC 3 cut(s) 520, 563, 645
ApaI GGGCCC 1 cut(s) 649
ApeKI GCWGC 3 cut(s) 212, 333, 749
ApoI RAATTY 3 cut(s) 55, 448, 742
AspLEI GCGC 2 cut(s) 580, 882
AspS9I GGNCC 2 cut(s) 645, 646
AsuHPI GGTGA 1 cut(s) 819
BaeGI GKGCMC 2 cut(s) 178, 649
BalI TGGCCA 1 cut(s) 565
BamHI GGATCC 1 cut(s) 652
BanII GRGCYC 1 cut(s) 649
BbsI GAAGAC 1 cut(s) 375
BbvI GCAGC 3 cut(s) 224, 320, 736
BccI CCATC 4 cut(s) 449, 512, 578, 665
BciT130I CCWGG 1 cut(s) 650
BclI TGATCA 1 cut(s) 250
BcoDI GTCTC 2 cut(s) 149, 765
BfaI CTAG 1 cut(s) 425
BfmI CTRYAG 1 cut(s) 513
BisI GCNGC 3 cut(s) 213, 334, 750
BlsI GCNGC 3 cut(s) 214, 335, 751
Bme1390I CCNGG 1 cut(s) 650
BmgT120I GGNCC 2 cut(s) 645, 646
BmiI GGNNCC 2 cut(s) 647, 654
BmrFI CCNGG 1 cut(s) 650
BmsI GCATC 5 cut(s) 37, 298, 449, 459, 565
BpiI GAAGAC 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 245
BsaHI GRCGYC 1 cut(s) 220
BsaI GGTCTC 2 cut(s) 149, 765
BsaJI CCNNGG 1 cut(s) 648
Bse1I ACTGG 1 cut(s) 760
Bse3DI GCAATG 1 cut(s) 792
BseBI CCWGG 1 cut(s) 650
BseDI CCNNGG 1 cut(s) 648
BseGI GGATG 6 cut(s) 460, 464, 474, 493, 580, 657
BseMI GCAATG 1 cut(s) 792
BseMII CTCAG 4 cut(s) 40, 606, 744, 873
BseNI ACTGG 1 cut(s) 760
BseSI GKGCMC 2 cut(s) 178, 649
BseXI GCAGC 3 cut(s) 224, 320, 736
BshFI GGCC 3 cut(s) 522, 565, 647
BsmAI GTCTC 2 cut(s) 149, 765
BsnI GGCC 3 cut(s) 522, 565, 647
Bso31I GGTCTC 2 cut(s) 149, 765
Bsp120I GGGCCC 1 cut(s) 645
Bsp1286I GDGCHC 2 cut(s) 178, 649
Bsp143I GATC 3 cut(s) 250, 652, 789
BspACI CCGC 2 cut(s) 662, 844
BspANI GGCC 3 cut(s) 522, 565, 647
BspCNI CTCAG 4 cut(s) 39, 605, 743, 872
BspLI GGNNCC 2 cut(s) 647, 654
BspPI GGATC 3 cut(s) 647, 660, 797
BspTNI GGTCTC 2 cut(s) 149, 765
BsrDI GCAATG 1 cut(s) 792
BsrI ACTGG 1 cut(s) 760
BssECI CCNNGG 1 cut(s) 648
BssMI GATC 3 cut(s) 250, 652, 789
BssNI GRCGYC 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 2 cut(s) 302, 834
BstACI GRCGYC 1 cut(s) 220
BstAPI GCANNNNNTGC 1 cut(s) 621
BstC8I GCNNGC 1 cut(s) 626
BstDEI CTNAG 4 cut(s) 26, 592, 730, 859
BstF5I GGATG 6 cut(s) 460, 464, 474, 493, 580, 657
BstHHI GCGC 2 cut(s) 580, 882
BstKTI GATC 3 cut(s) 253, 655, 792
BstMAI GTCTC 2 cut(s) 149, 765
BstMBI GATC 3 cut(s) 250, 652, 789
BstMWI GCNNNNNNNGC 2 cut(s) 161, 621
BstNI CCWGG 1 cut(s) 650
BstSCI CCNGG 1 cut(s) 648
BstSFI CTRYAG 1 cut(s) 513
BstSLI GKGCMC 2 cut(s) 178, 649
BstV1I GCAGC 3 cut(s) 224, 320, 736
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 1 cut(s) 652
BstYI RGATCY 1 cut(s) 652
BsuRI GGCC 3 cut(s) 522, 565, 647
BtsCI GGATG 6 cut(s) 460, 464, 474, 493, 580, 657
BtsI GCAGTG 1 cut(s) 875
BtsIMutI CAGTG 1 cut(s) 875
Cac8I GCNNGC 1 cut(s) 626
CaiI CAGNNNCTG 1 cut(s) 838
CfoI GCGC 2 cut(s) 580, 882
Cfr13I GGNCC 2 cut(s) 645, 646
CseI GACGC 2 cut(s) 228, 950
CviAII CATG 2 cut(s) 473, 883
DdeI CTNAG 4 cut(s) 26, 592, 730, 859
DpnI GATC 3 cut(s) 252, 654, 791
DpnII GATC 3 cut(s) 250, 652, 789
DraI TTTAAA 1 cut(s) 555
DraIII CACNNNGTG 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 563
Eco24I GRGCYC 1 cut(s) 649
Eco31I GGTCTC 2 cut(s) 149, 765
EcoO109I RGGNCCY 1 cut(s) 645
EcoRII CCWGG 1 cut(s) 648
EcoT22I ATGCAT 1 cut(s) 474
EcoT38I GRGCYC 1 cut(s) 649
FaeI CATG 2 cut(s) 476, 886
FalI AAGNNNNNCTT 4 cut(s) 67, 99, 305, 337
FatI CATG 2 cut(s) 472, 882
FauI CCCGC 1 cut(s) 669
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 3 cut(s) 213, 334, 750
FokI GGATG 6 cut(s) 467, 471, 481, 500, 587, 644
FriOI GRGCYC 1 cut(s) 649
Fsp4HI GCNGC 3 cut(s) 213, 334, 750
FspAI RTGCGCAY 1 cut(s) 579
FspBI CTAG 1 cut(s) 425
FspI TGCGCA 2 cut(s) 579, 881
GlaI GCGC 2 cut(s) 579, 881
GluI GCNGC 3 cut(s) 213, 334, 750
HaeIII GGCC 3 cut(s) 522, 565, 647
HgaI GACGC 2 cut(s) 228, 950
HhaI GCGC 2 cut(s) 580, 882
Hin1I GRCGYC 1 cut(s) 220
Hin1II CATG 2 cut(s) 476, 886
Hin6I GCGC 2 cut(s) 578, 880
HinP1I GCGC 2 cut(s) 578, 880
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 4 cut(s) 438, 700, 824, 919
HpaI GTTAAC 1 cut(s) 415
HphI GGTGA 1 cut(s) 819
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 3 cut(s) 255, 454, 862
Hpy188III TCNNGA 4 cut(s) 407, 485, 690, 770
Hpy8I GTNNAC 1 cut(s) 415
Hpy99I CGWCG 2 cut(s) 222, 574
HpyAV CCTTC 1 cut(s) 907
HpyCH4III ACNGT 2 cut(s) 302, 834
HpyCH4IV ACGT 1 cut(s) 238
HpyCH4V TGCA 4 cut(s) 472, 624, 797, 803
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 621
HpyF3I CTNAG 4 cut(s) 26, 592, 730, 859
HpySE526I ACGT 1 cut(s) 238
Hsp92I GRCGYC 1 cut(s) 220
Hsp92II CATG 2 cut(s) 476, 886
HspAI GCGC 2 cut(s) 578, 880
Ksp22I TGATCA 1 cut(s) 250
KspAI GTTAAC 1 cut(s) 415
Kzo9I GATC 3 cut(s) 250, 652, 789
LpnPI CCDG 7 cut(s) 126, 357, 448, 635, 662, 741, 851
Lsp1109I GCAGC 3 cut(s) 224, 320, 736
LweI GCATC 5 cut(s) 37, 298, 449, 459, 565
MaeI CTAG 1 cut(s) 425
MaeII ACGT 1 cut(s) 238
MaeIII GTNAC 3 cut(s) 231, 302, 825
MalI GATC 3 cut(s) 252, 654, 791
MboI GATC 3 cut(s) 250, 652, 789
MboII GAAGA 6 cut(s) 53, 56, 79, 209, 359, 380
MfeI CAATTG 3 cut(s) 150, 362, 501
MflI RGATCY 1 cut(s) 652
MhlI GDGCHC 2 cut(s) 178, 649
MlsI TGGCCA 1 cut(s) 565
MluCI AATT 9 cut(s) 55, 100, 150, 362, 448, 501, 666, 742, 924
MluNI TGGCCA 1 cut(s) 565
MlyI GAGTC 3 cut(s) 447, 694, 833
MnlI CCTC 4 cut(s) 165, 533, 538, 636
Mox20I TGGCCA 1 cut(s) 565
Mph1103I ATGCAT 1 cut(s) 474
MscI TGGCCA 1 cut(s) 565
MseI TTAA 7 cut(s) 281, 414, 554, 705, 735, 927, 948
MslI CAYNNNNRTG 1 cut(s) 713
Msp20I TGGCCA 1 cut(s) 565
MspR9I CCNGG 1 cut(s) 650
MunI CAATTG 3 cut(s) 150, 362, 501
MvaI CCWGG 1 cut(s) 650
MwoI GCNNNNNNNGC 2 cut(s) 161, 621
NdeII GATC 3 cut(s) 250, 652, 789
NlaIII CATG 2 cut(s) 476, 886
NlaIV GGNNCC 2 cut(s) 647, 654
NmuCI GTSAC 2 cut(s) 231, 825
NsbI TGCGCA 2 cut(s) 579, 881
NsiI ATGCAT 1 cut(s) 474
PfeI GAWTC 1 cut(s) 919
PkrI GCNGC 3 cut(s) 214, 335, 751
PleI GAGTC 3 cut(s) 446, 694, 832
PpsI GAGTC 3 cut(s) 446, 694, 832
Psp6I CCWGG 1 cut(s) 648
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 2 cut(s) 647, 654
PspOMI GGGCCC 1 cut(s) 645
PspPI GGNCC 2 cut(s) 645, 646
PstNI CAGNNNCTG 1 cut(s) 838
PsuI RGATCY 1 cut(s) 652
RseI CAYNNNNRTG 1 cut(s) 713
SaqAI TTAA 7 cut(s) 281, 414, 554, 705, 735, 927, 948
SatI GCNGC 3 cut(s) 213, 334, 750
Sau3AI GATC 3 cut(s) 250, 652, 789
Sau96I GGNCC 2 cut(s) 645, 646
SchI GAGTC 3 cut(s) 447, 694, 833
ScrFI CCNGG 1 cut(s) 650
SduI GDGCHC 2 cut(s) 178, 649
SetI ASST 7 cut(s) 126, 217, 241, 249, 287, 630, 725
SfaNI GCATC 5 cut(s) 37, 298, 449, 459, 565
SfcI CTRYAG 1 cut(s) 513
SmiMI CAYNNNNRTG 1 cut(s) 713
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 9 cut(s) 55, 100, 150, 362, 448, 501, 666, 742, 924
SsiI CCGC 2 cut(s) 662, 844
SspMI CTAG 1 cut(s) 425
StyD4I CCNGG 1 cut(s) 648
TaaI ACNGT 2 cut(s) 302, 834
TaiI ACGT 1 cut(s) 241
TaqI TCGA 3 cut(s) 217, 406, 769
TasI AATT 9 cut(s) 55, 100, 150, 362, 448, 501, 666, 742, 924
TfiI GAWTC 1 cut(s) 919
Tru1I TTAA 7 cut(s) 281, 414, 554, 705, 735, 927, 948
Tru9I TTAA 7 cut(s) 281, 414, 554, 705, 735, 927, 948
TscAI CASTG 1 cut(s) 882
TseFI GTSAC 2 cut(s) 231, 825
TseI GCWGC 3 cut(s) 212, 333, 749
Tsp45I GTSAC 2 cut(s) 231, 825
TspDTI ATGAA 3 cut(s) 11, 731, 751
TspGWI ACGGA 2 cut(s) 587, 802
TspRI CASTG 1 cut(s) 882
XapI RAATTY 3 cut(s) 55, 448, 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 906
XspI CTAG 1 cut(s) 425
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.