Rmu_sc0001403.1_g000035

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001403.1
OG0023043
Physical Location & Seq
Forward (+)
148830 .. 149279
450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001403.1_g000035.1.cds

Sequence Viewer

Length: 366 bp
atgcacccaaacaggaaccagttgacaacagtcctattaaacccgggacttctggatctgctgctaaagcaaacccgtctgcctccaagtcggcgaagggaaagagaattaaatggtgatgacgctaaagcatccaacagtgcacctcccaagaaggtgaagaccgaaaatgagcaaaaatcagtcaaagaggagcctgcttctgaaagtagtgtgcctgcaaaaggtactgctgcatcctcgaaaacgggttcaacaccatctggtggtcctacaggtcctgtatctgaggaagaaattagggcagttttgatgcaaaagtcacccatcaccacacgcgatcttgttaataacttccccggatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

121

Amino Acids

12.98

Weight (kDa)

9.63

Isoelectric Point (pI)

64.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023043)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8174024.1_g000001 Rmu_sc0000405.1_g000010 Rmu_sc0001403.1_g000035 Rmu_sc0003131.1_g000006

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 266
AccII CGCG 1 cut(s) 339
AclWI GGATC 1 cut(s) 63
AfaI GTAC 1 cut(s) 229
AfiI CCNNNNNNNGG 2 cut(s) 224, 266
AgsI TTSAA 1 cut(s) 255
Alw21I GWGCWC 1 cut(s) 145
Alw44I GTGCAC 1 cut(s) 141
AlwI GGATC 1 cut(s) 63
AlwNI CAGNNNCTG 1 cut(s) 281
Ama87I CYCGRG 1 cut(s) 43
ApaLI GTGCAC 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 61, 233
AspS9I GGNCC 2 cut(s) 269, 278
AsuC2I CCSGG 3 cut(s) 44, 45, 360
AsuHPI GGTGA 4 cut(s) 128, 169, 315, 322
AvaI CYCGRG 1 cut(s) 43
AvaII GGWCC 2 cut(s) 269, 278
BaeGI GKGCMC 1 cut(s) 145
BbsI GAAGAC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 145
BbvI GCAGC 2 cut(s) 48, 220
BccI CCATC 2 cut(s) 268, 335
BcnI CCSGG 3 cut(s) 44, 45, 360
BfmI CTRYAG 1 cut(s) 273
BisI GCNGC 2 cut(s) 62, 234
BlsI GCNGC 2 cut(s) 63, 235
Bme1390I CCNGG 3 cut(s) 44, 45, 360
Bme18I GGWCC 2 cut(s) 269, 278
BmeT110I CYCGRG 1 cut(s) 43
BmgT120I GGNCC 2 cut(s) 269, 278
BmiI GGNNCC 2 cut(s) 17, 195
BmrFI CCNGG 3 cut(s) 44, 45, 360
BmsI GCATC 3 cut(s) 140, 245, 303
BoxI GACNNNNGTC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 167
BpuMI CCSGG 3 cut(s) 44, 45, 360
BsaJI CCNNGG 2 cut(s) 43, 358
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 266
Bse1I ACTGG 1 cut(s) 19
BseDI CCNNGG 2 cut(s) 43, 358
BseGI GGATG 2 cut(s) 131, 236
BseLI CCNNNNNNNGG 2 cut(s) 224, 266
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 1 cut(s) 19
BseRI GAGGAG 1 cut(s) 206
BseSI GKGCMC 1 cut(s) 145
BseXI GCAGC 2 cut(s) 48, 220
Bsh1236I CGCG 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 145
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 2 cut(s) 44, 360
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 224, 266
BsmFI GGGAC 1 cut(s) 60
BsoBI CYCGRG 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 145
Bsp143I GATC 2 cut(s) 55, 340
BspCNI CTCAG 1 cut(s) 280
BspFNI CGCG 1 cut(s) 339
BspLI GGNNCC 2 cut(s) 17, 195
BspPI GGATC 1 cut(s) 63
BsrI ACTGG 1 cut(s) 19
BssECI CCNNGG 2 cut(s) 43, 358
BssMI GATC 2 cut(s) 55, 340
Bst4CI ACNGT 2 cut(s) 31, 140
BstC8I GCNNGC 2 cut(s) 198, 219
BstDEI CTNAG 1 cut(s) 288
BstENI CCTNNNNNAGG 1 cut(s) 222
BstF5I GGATG 2 cut(s) 131, 236
BstFNI CGCG 1 cut(s) 339
BstKTI GATC 2 cut(s) 58, 343
BstMBI GATC 2 cut(s) 55, 340
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstPAI GACNNNNGTC 1 cut(s) 29
BstSCI CCNGG 3 cut(s) 42, 43, 358
BstSFI CTRYAG 1 cut(s) 273
BstSLI GKGCMC 1 cut(s) 145
BstUI CGCG 1 cut(s) 339
BstV1I GCAGC 2 cut(s) 48, 220
BstV2I GAAGAC 1 cut(s) 167
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BtsCI GGATG 2 cut(s) 131, 236
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 2 cut(s) 198, 219
CaiI CAGNNNCTG 1 cut(s) 281
Cfr13I GGNCC 2 cut(s) 269, 278
Cfr9I CCCGGG 1 cut(s) 43
CseI GACGC 1 cut(s) 131
Csp6I GTAC 1 cut(s) 228
CviJI RGCY 1 cut(s) 196
CviKI_1 RGCY 1 cut(s) 196
CviQI GTAC 1 cut(s) 228
DdeI CTNAG 1 cut(s) 288
DpnI GATC 2 cut(s) 57, 342
DpnII GATC 2 cut(s) 55, 340
Eco47I GGWCC 2 cut(s) 269, 278
Eco88I CYCGRG 1 cut(s) 43
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoO109I RGGNCCY 1 cut(s) 278
FaqI GGGAC 1 cut(s) 60
Fnu4HI GCNGC 2 cut(s) 62, 234
FokI GGATG 2 cut(s) 118, 223
Fsp4HI GCNGC 2 cut(s) 62, 234
GluI GCNGC 2 cut(s) 62, 234
HapII CCGG 2 cut(s) 44, 360
HgaI GACGC 1 cut(s) 131
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HpaII CCGG 2 cut(s) 44, 360
HphI GGTGA 4 cut(s) 128, 169, 315, 322
Hpy166II GTNNAC 2 cut(s) 24, 143
Hpy188I TCNGA 2 cut(s) 205, 289
Hpy188III TCNNGA 1 cut(s) 53
Hpy8I GTNNAC 2 cut(s) 24, 143
HpyAV CCTTC 2 cut(s) 90, 148
HpyCH4III ACNGT 2 cut(s) 31, 140
HpyCH4V TGCA 5 cut(s) 4, 143, 221, 236, 316
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
HpyF3I CTNAG 1 cut(s) 288
Kzo9I GATC 2 cut(s) 55, 340
LmnI GCTCC 1 cut(s) 193
LpnPI CCDG 8 cut(s) 32, 38, 57, 210, 231, 249, 261, 294
Lsp1109I GCAGC 2 cut(s) 48, 220
LweI GCATC 3 cut(s) 140, 245, 303
MaeIII GTNAC 1 cut(s) 321
MalI GATC 2 cut(s) 57, 342
MboI GATC 2 cut(s) 55, 340
MboII GAAGA 2 cut(s) 172, 305
MflI RGATCY 1 cut(s) 55
MhlI GDGCHC 1 cut(s) 145
MluCI AATT 2 cut(s) 107, 297
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 5 cut(s) 93, 156, 184, 250, 283
MseI TTAA 3 cut(s) 38, 110, 348
MspI CCGG 2 cut(s) 44, 360
MspR9I CCNGG 3 cut(s) 44, 45, 360
MvnI CGCG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 67
NciI CCSGG 3 cut(s) 44, 45, 360
NdeII GATC 2 cut(s) 55, 340
NlaIV GGNNCC 2 cut(s) 17, 195
NmuCI GTSAC 1 cut(s) 321
PflMI CCANNNNNTGG 1 cut(s) 266
PkrI GCNGC 2 cut(s) 63, 235
PpuMI RGGWCCY 1 cut(s) 278
PshAI GACNNNNGTC 1 cut(s) 29
Psp5II RGGWCCY 1 cut(s) 278
PspN4I GGNNCC 2 cut(s) 17, 195
PspPI GGNCC 2 cut(s) 269, 278
PspPPI RGGWCCY 1 cut(s) 278
PstNI CAGNNNCTG 1 cut(s) 281
PsuI RGATCY 1 cut(s) 55
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
SaqAI TTAA 3 cut(s) 38, 110, 348
SatI GCNGC 2 cut(s) 62, 234
Sau3AI GATC 2 cut(s) 55, 340
Sau96I GGNCC 2 cut(s) 269, 278
ScrFI CCNGG 3 cut(s) 44, 45, 360
SduI GDGCHC 1 cut(s) 145
SetI ASST 4 cut(s) 148, 159, 229, 280
SfaNI GCATC 3 cut(s) 140, 245, 303
SfcI CTRYAG 1 cut(s) 273
SinI GGWCC 2 cut(s) 269, 278
SmaI CCCGGG 1 cut(s) 45
Sse9I AATT 2 cut(s) 107, 297
StyD4I CCNGG 3 cut(s) 42, 43, 358
TaaI ACNGT 2 cut(s) 31, 140
TaqI TCGA 1 cut(s) 242
TaqII GACCGA 1 cut(s) 179
TasI AATT 2 cut(s) 107, 297
Tru1I TTAA 3 cut(s) 38, 110, 348
Tru9I TTAA 3 cut(s) 38, 110, 348
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 321
TseI GCWGC 2 cut(s) 61, 233
Tsp45I GTSAC 1 cut(s) 321
TspMI CCCGGG 1 cut(s) 43
TspRI CASTG 1 cut(s) 145
Van91I CCANNNNNTGG 1 cut(s) 266
VneI GTGCAC 1 cut(s) 141
VpaK11BI GGWCC 2 cut(s) 269, 278
XagI CCTNNNNNAGG 1 cut(s) 222
XmaI CCCGGG 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.