Rmu_sc0001477.1_g000014

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001477.1
OG0031135
Physical Location & Seq
Reverse (-)
59236 .. 65353
6118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001477.1_g000014.1.cds

Sequence Viewer

Length: 1056 bp
atggaaaaaaccatgtcccagaagaagaaatacatgattcgaatagagcgggagtccgtggttccaattctcaagcccaagctcatcatgtccttggccaacctcattggtttgttctcaaccctgcttttgctcttgattgggtattggattccagatgggggcagcaacaataattttgaggttcttgtaattgaataccaaaaggaatcactggaggtgccttctacggattgtcttcttgcaaatactcaagtggacgctgtacaagaagaaacaagttacaagaagactcgatcaaagaggcctagaggaatacatggaaagaagccaatagaagaatctgttgaaaacttggatgacagcaacaatacgggtgatgatcttgatgatcaatatccaaaggaattattggacttggagggcatggattgcgctcctgaaagtactcaaggagatgagttacaagatgatagtggtaagaaaagaaaaacgtacagaaagactcgagcaaagattcctacagaaaaactggaaacatcagggttgcatgctatggattgtgaagatgatatgctaaaagccatcatcatagatgctattgagatgaagagacaagtgagtccaaccatcaacttcaagggcaaggacatgaggaaatcattttctgcataccgtgaggtaaggatgaacaagaacatcagcattgcagttgaagaggaaaaaagtgccactccgtcggctatgctcggatattgccgttgtagagatgttgccggtgtagcgaaatggcagccggacttcgaaagggaaggaggaacagtggcggccgggaatggcatttcccggtggttgcagagggaagagagaatgagagaggtcggggaaaagagagagagggagaagaaagggtcgtgcggcccagagaaagaagatggaaaaaatgaggggctgcttagggttaatttgtctaaatacgtgatattaggaaaagtcgatttttcccataacaaattcccgaaatcttcaattgaaataaacttccatacaccgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

351

Amino Acids

39.82

Weight (kDa)

6.52

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031135)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001477.1_g000014 Rmu_sc0012764.1_g000006

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 621
AccB1I GGYRCC 1 cut(s) 220
AccBSI CCGCTC 1 cut(s) 49
AciI CCGC 3 cut(s) 49, 827, 918
AcoI YGGCCR 2 cut(s) 96, 828
AcsI RAATTY 1 cut(s) 1013
AfaI GTAC 3 cut(s) 267, 448, 497
AfiI CCNNNNNNNGG 1 cut(s) 161
AgsI TTSAA 6 cut(s) 197, 350, 640, 716, 1029, 1034
AluBI AGCT 1 cut(s) 82
AluI AGCT 1 cut(s) 82
Alw26I GTCTC 1 cut(s) 607
Ama87I CYCGRG 1 cut(s) 507
AoxI GGCC 4 cut(s) 96, 305, 828, 919
ApeKI GCWGC 3 cut(s) 165, 793, 952
ApoI RAATTY 1 cut(s) 1013
AspLEI GCGC 1 cut(s) 437
AspS9I GGNCC 1 cut(s) 920
AsuC2I CCSGG 2 cut(s) 832, 847
AsuHPI GGTGA 1 cut(s) 389
AsuII TTCGAA 2 cut(s) 40, 804
AvaI CYCGRG 1 cut(s) 507
BalI TGGCCA 1 cut(s) 98
BanI GGYRCC 1 cut(s) 220
BarI GAAGNNNNNNTAC 2 cut(s) 14, 46
BbsI GAAGAC 2 cut(s) 230, 296
BbvI GCAGC 3 cut(s) 177, 805, 939
BccI CCATC 4 cut(s) 152, 593, 638, 929
BceAI ACGGC 1 cut(s) 744
BclI TGATCA 1 cut(s) 391
BcnI CCSGG 2 cut(s) 832, 847
BcoDI GTCTC 1 cut(s) 607
BfaI CTAG 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 522
BisI GCNGC 5 cut(s) 166, 794, 828, 919, 953
BlsI GCNGC 5 cut(s) 167, 795, 829, 920, 954
BmcAI AGTACT 1 cut(s) 448
Bme1390I CCNGG 2 cut(s) 832, 847
BmeT110I CYCGRG 1 cut(s) 507
BmgT120I GGNCC 1 cut(s) 920
BmiI GGNNCC 2 cut(s) 63, 222
BmrFI CCNGG 2 cut(s) 832, 847
BmsI GCATC 1 cut(s) 586
BpiI GAAGAC 2 cut(s) 230, 296
BpmI CTGGAG 1 cut(s) 236
Bpu10I CCTNAGC 1 cut(s) 956
Bpu14I TTCGAA 2 cut(s) 40, 804
BpuEI CTTGAG 3 cut(s) 56, 237, 435
BpuMI CCSGG 2 cut(s) 832, 847
BsaAI YACGTR 1 cut(s) 979
BsaBI GATNNNNATC 1 cut(s) 396
BsaJI CCNNGG 2 cut(s) 57, 93
BsaXI ACNNNNNCTCC 2 cut(s) 807, 837
Bsc4I CCNNNNNNNGG 1 cut(s) 161
Bse118I RCCGGY 1 cut(s) 776
Bse1I ACTGG 2 cut(s) 219, 537
Bse3DI GCAATG 1 cut(s) 705
Bse8I GATNNNNATC 1 cut(s) 396
BseDI CCNNGG 2 cut(s) 57, 93
BseGI GGATG 2 cut(s) 364, 693
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMI GCAATG 1 cut(s) 705
BseNI ACTGG 2 cut(s) 219, 537
BseX3I CGGCCG 1 cut(s) 828
BseXI GCAGC 3 cut(s) 177, 805, 939
Bsh1285I CGRYCG 1 cut(s) 831
BshFI GGCC 4 cut(s) 98, 307, 830, 921
BshNI GGYRCC 1 cut(s) 220
BsiEI CGRYCG 1 cut(s) 831
BsiHKCI CYCGRG 1 cut(s) 507
BsiSI CCGG 4 cut(s) 777, 797, 831, 847
BslI CCNNNNNNNGG 1 cut(s) 161
BsmAI GTCTC 1 cut(s) 607
BsnI GGCC 4 cut(s) 98, 307, 830, 921
BsoBI CYCGRG 1 cut(s) 507
Bsp119I TTCGAA 2 cut(s) 40, 804
Bsp1407I TGTACA 1 cut(s) 265
Bsp143I GATC 3 cut(s) 296, 382, 391
BspACI CCGC 3 cut(s) 49, 827, 918
BspANI GGCC 4 cut(s) 98, 307, 830, 921
BspLI GGNNCC 2 cut(s) 63, 222
BspT104I TTCGAA 2 cut(s) 40, 804
BspT107I GGYRCC 1 cut(s) 220
BsrBI CCGCTC 1 cut(s) 49
BsrDI GCAATG 1 cut(s) 705
BsrFI RCCGGY 1 cut(s) 776
BsrGI TGTACA 1 cut(s) 265
BsrI ACTGG 2 cut(s) 219, 537
BssAI RCCGGY 1 cut(s) 776
BssECI CCNNGG 2 cut(s) 57, 93
BssMI GATC 3 cut(s) 296, 382, 391
BssT1I CCWWGG 1 cut(s) 93
Bst4CI ACNGT 3 cut(s) 677, 823, 1053
Bst6I CTCTTC 3 cut(s) 605, 711, 858
BstAPI GCANNNNNTGC 1 cut(s) 432
BstAUI TGTACA 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 979
BstBI TTCGAA 2 cut(s) 40, 804
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 1 cut(s) 956
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 2 cut(s) 364, 693
BstHHI GCGC 1 cut(s) 437
BstKTI GATC 3 cut(s) 299, 385, 394
BstMAI GTCTC 1 cut(s) 607
BstMBI GATC 3 cut(s) 296, 382, 391
BstMCI CGRYCG 1 cut(s) 831
BstMWI GCNNNNNNNGC 2 cut(s) 432, 782
BstNSI RCATGY 1 cut(s) 554
BstSCI CCNGG 2 cut(s) 830, 845
BstSFI CTRYAG 1 cut(s) 522
BstV1I GCAGC 3 cut(s) 177, 805, 939
BstV2I GAAGAC 2 cut(s) 230, 296
BstZI CGGCCG 1 cut(s) 828
BsuRI GGCC 4 cut(s) 98, 307, 830, 921
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 2 cut(s) 364, 693
BtsIMutI CAGTG 2 cut(s) 212, 828
Cac8I GCNNGC 1 cut(s) 552
CfoI GCGC 1 cut(s) 437
Cfr10I RCCGGY 1 cut(s) 776
Cfr13I GGNCC 1 cut(s) 920
CseI GACGC 1 cut(s) 269
Csp6I GTAC 3 cut(s) 266, 447, 496
CviAII CATG 7 cut(s) 13, 34, 88, 320, 427, 551, 652
CviQI GTAC 3 cut(s) 266, 447, 496
DdeI CTNAG 1 cut(s) 956
DpnI GATC 3 cut(s) 298, 384, 393
DpnII GATC 3 cut(s) 296, 382, 391
DrdI GACNNNNNNGTC 1 cut(s) 621
DseDI GACNNNNNNGTC 1 cut(s) 621
EaeI YGGCCR 2 cut(s) 96, 828
EagI CGGCCG 1 cut(s) 828
Eam1104I CTCTTC 3 cut(s) 605, 711, 858
EarI CTCTTC 3 cut(s) 605, 711, 858
EclXI CGGCCG 1 cut(s) 828
Eco130I CCWWGG 1 cut(s) 93
Eco147I AGGCCT 1 cut(s) 307
Eco52I CGGCCG 1 cut(s) 828
Eco88I CYCGRG 1 cut(s) 507
EcoT14I CCWWGG 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 7 cut(s) 16, 37, 91, 323, 430, 554, 655
FatI CATG 7 cut(s) 12, 33, 87, 319, 426, 550, 651
FauI CCCGC 1 cut(s) 42
FbaI TGATCA 1 cut(s) 391
Fnu4HI GCNGC 5 cut(s) 166, 794, 828, 919, 953
FokI GGATG 2 cut(s) 371, 700
Fsp4HI GCNGC 5 cut(s) 166, 794, 828, 919, 953
FspBI CTAG 1 cut(s) 309
GlaI GCGC 1 cut(s) 436
GluI GCNGC 5 cut(s) 166, 794, 828, 919, 953
GsuI CTGGAG 1 cut(s) 236
HaeIII GGCC 4 cut(s) 98, 307, 830, 921
HapII CCGG 4 cut(s) 777, 797, 831, 847
HgaI GACGC 1 cut(s) 269
HhaI GCGC 1 cut(s) 437
Hin1II CATG 7 cut(s) 16, 37, 91, 323, 430, 554, 655
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HinfI GANTC 9 cut(s) 37, 53, 151, 209, 292, 341, 505, 517, 622
HpaII CCGG 4 cut(s) 777, 797, 831, 847
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 1 cut(s) 752
Hpy188III TCNNGA 5 cut(s) 136, 155, 386, 440, 1018
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 1 cut(s) 742
HpyAV CCTTC 2 cut(s) 234, 806
HpyCH4III ACNGT 3 cut(s) 677, 823, 1053
HpyCH4IV ACGT 2 cut(s) 494, 978
HpyCH4V TGCA 5 cut(s) 245, 550, 671, 710, 856
HpyF10VI GCNNNNNNNGC 2 cut(s) 432, 782
HpyF3I CTNAG 1 cut(s) 956
HpySE526I ACGT 2 cut(s) 494, 978
Hsp92II CATG 7 cut(s) 16, 37, 91, 323, 430, 554, 655
HspAI GCGC 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 3 cut(s) 296, 382, 391
LmnI GCTCC 1 cut(s) 442
Lsp1109I GCAGC 3 cut(s) 177, 805, 939
LweI GCATC 1 cut(s) 586
MaeI CTAG 1 cut(s) 309
MaeII ACGT 2 cut(s) 494, 978
MaeIII GTNAC 2 cut(s) 281, 462
MalI GATC 3 cut(s) 298, 384, 393
MbiI CCGCTC 1 cut(s) 49
MboI GATC 3 cut(s) 296, 382, 391
MfeI CAATTG 1 cut(s) 1029
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 7 cut(s) 66, 175, 192, 407, 964, 1013, 1029
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 4 cut(s) 62, 286, 499, 631
MmeI TCCRAC 1 cut(s) 650
Mox20I TGGCCA 1 cut(s) 98
MscI TGGCCA 1 cut(s) 98
MseI TTAA 1 cut(s) 963
Msp20I TGGCCA 1 cut(s) 98
MspI CCGG 4 cut(s) 777, 797, 831, 847
MspR9I CCNGG 2 cut(s) 832, 847
MunI CAATTG 1 cut(s) 1029
MwoI GCNNNNNNNGC 2 cut(s) 432, 782
NciI CCSGG 2 cut(s) 832, 847
NdeII GATC 3 cut(s) 296, 382, 391
NlaIII CATG 7 cut(s) 16, 37, 91, 323, 430, 554, 655
NlaIV GGNNCC 2 cut(s) 63, 222
NspI RCATGY 1 cut(s) 554
NspV TTCGAA 2 cut(s) 40, 804
PaeI GCATGC 1 cut(s) 554
PaeR7I CTCGAG 1 cut(s) 507
PceI AGGCCT 1 cut(s) 307
PfeI GAWTC 5 cut(s) 37, 151, 209, 341, 517
PkrI GCNGC 5 cut(s) 167, 795, 829, 920, 954
PleI GAGTC 4 cut(s) 61, 286, 499, 630
PpsI GAGTC 4 cut(s) 61, 286, 499, 630
Ppu21I YACGTR 1 cut(s) 979
PspN4I GGNNCC 2 cut(s) 63, 222
PspPI GGNCC 1 cut(s) 920
PspXI VCTCGAGB 1 cut(s) 507
RsaI GTAC 3 cut(s) 267, 448, 497
RsaNI GTAC 3 cut(s) 266, 447, 496
SaqAI TTAA 1 cut(s) 963
SatI GCNGC 5 cut(s) 166, 794, 828, 919, 953
Sau3AI GATC 3 cut(s) 296, 382, 391
Sau96I GGNCC 1 cut(s) 920
ScaI AGTACT 1 cut(s) 448
SchI GAGTC 4 cut(s) 62, 286, 499, 631
ScrFI CCNGG 2 cut(s) 832, 847
SetI ASST 8 cut(s) 84, 105, 186, 222, 497, 684, 882, 981
SfaNI GCATC 1 cut(s) 586
SfcI CTRYAG 1 cut(s) 522
Sfr274I CTCGAG 1 cut(s) 507
SfuI TTCGAA 2 cut(s) 40, 804
SlaI CTCGAG 1 cut(s) 507
SmlI CTYRAG 4 cut(s) 71, 252, 450, 507
SmoI CTYRAG 4 cut(s) 71, 252, 450, 507
SphI GCATGC 1 cut(s) 554
Sse9I AATT 7 cut(s) 66, 175, 192, 407, 964, 1013, 1029
SseBI AGGCCT 1 cut(s) 307
SsiI CCGC 3 cut(s) 49, 827, 918
SspMI CTAG 1 cut(s) 309
StuI AGGCCT 1 cut(s) 307
StyD4I CCNGG 2 cut(s) 830, 845
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 3 cut(s) 677, 823, 1053
TaiI ACGT 2 cut(s) 497, 981
TaqI TCGA 5 cut(s) 40, 295, 508, 804, 996
TasI AATT 7 cut(s) 66, 175, 192, 407, 964, 1013, 1029
TatI WGTACW 2 cut(s) 265, 446
TauI GCSGC 2 cut(s) 830, 921
TfiI GAWTC 5 cut(s) 37, 151, 209, 341, 517
Tru1I TTAA 1 cut(s) 963
Tru9I TTAA 1 cut(s) 963
TscAI CASTG 2 cut(s) 219, 828
TseI GCWGC 3 cut(s) 165, 793, 952
TspDTI ATGAA 2 cut(s) 623, 704
TspGWI ACGGA 3 cut(s) 46, 245, 726
TspRI CASTG 2 cut(s) 219, 828
XapI RAATTY 1 cut(s) 1013
XceI RCATGY 1 cut(s) 554
XhoI CTCGAG 1 cut(s) 507
XspI CTAG 1 cut(s) 309
ZrmI AGTACT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.