Rmu_sc0001543.1_g000001

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001543.1
OG0031138
Physical Location & Seq
Forward (+)
1 .. 2311
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001543.1_g000001.1.cds

Sequence Viewer

Length: 1195 bp
acaagatgtcatcagacttttacatgttgatgaaaatattgttcgcatggtaggcatatgggggactggtgggataggaaagaccacaattgcgaaagatgtgtttaattcaattcgccataagtttaaaggtagctgtttcttggcagatgttagatcaaatggcctaacccaattgcaagagacacttttgtctgatatcttaagtgactcaactttgaaggtgtgcagtgttgataaaggagtcgttgatgaagtatacgaggtcaaaatgttagactgtagtcgagctttggagttgtttagcttgaatgcattcaaaagaaatggacctccagaagattatctggaacttgcccaacgtgctatacactatgcccaaggccttccattggctttgatagttttaggctctcatctatttcgtagaagcaaagaggagttggaagctacaataggtagttgtaaaggaggtcctcagacggagatacaagatgttcttagactaagttatgatgctcttggaccagatttaaaagaactatttcttgatatcgcttgcttctttaaaggtatggaggtccaccatgtgaaacaaatactagaagcttgctacgacctcagatcagtgattggtattgcacaactacaagaaaaagtcttaattagaattgagagatatgagccttggacaaatggaatagacaaaggtcgtatttggatgaatgacttgatagaagaaatgggtaaagacattgtgtatcaagagtcacccggtgagcctgggaaacgcagtagagtctggagtggagatgatgtcgaccacattctaacaaataatacgaattttccaaggctaacatatatgaatttaaggggttgtgaatccctaacagaactcccagacttcaggggaatccccaacctaaaagagttggatctatctgactgtaagagtttagttgaggttcccgatttcgttggactccttcgtaaccttgttaccttgaatgtggaccgctgctctaaccttattatgtttccaagaaaaatcaacttgaaatccgtggaaactatttctgttagctattgcgagcttgaggaattttcagaagttggggaagagataggttcattgacaaatttggatctatcaggaacttgcattcgtcaatcacaaaacttattgggttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

397

Amino Acids

44.64

Weight (kDa)

5.06

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031138)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001543.1_g000001 Rmu_sc0013954.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AccI GTMKAC 2 cut(s) 259, 820
AciI CCGC 1 cut(s) 1019
AclWI GGATC 2 cut(s) 946, 1156
AcsI RAATTY 4 cut(s) 845, 869, 1104, 1142
AcuI CTGAAG 1 cut(s) 893
AdeI CACNNNGTG 2 cut(s) 590, 777
AfiI CCNNNNNNNGG 1 cut(s) 392
AflII CTTAAG 1 cut(s) 203
AflIII ACRYGT 1 cut(s) 23
AgsI TTSAA 6 cut(s) 112, 221, 311, 320, 1010, 1061
AjnI CCWGG 1 cut(s) 782
AluBI AGCT 7 cut(s) 136, 291, 307, 450, 609, 1087, 1097
AluI AGCT 7 cut(s) 136, 291, 307, 450, 609, 1087, 1097
Alw26I GTCTC 1 cut(s) 177
AlwI GGATC 2 cut(s) 946, 1156
AoxI GGCC 2 cut(s) 164, 383
ApeKI GCWGC 1 cut(s) 1021
ApoI RAATTY 4 cut(s) 845, 869, 1104, 1142
Asp700I GAANNNNTTC 2 cut(s) 315, 544
AspS9I GGNCC 5 cut(s) 330, 474, 525, 581, 1016
AsuC2I CCSGG 1 cut(s) 775
AsuHPI GGTGA 2 cut(s) 763, 789
AvaII GGWCC 5 cut(s) 330, 474, 525, 581, 1016
BbvI GCAGC 1 cut(s) 1008
BciT130I CCWGG 1 cut(s) 784
BcnI CCSGG 1 cut(s) 775
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 1 cut(s) 603
BfmI CTRYAG 1 cut(s) 281
BfrI CTTAAG 1 cut(s) 203
BisI GCNGC 1 cut(s) 1022
BlsI GCNGC 1 cut(s) 1023
Bme1390I CCNGG 2 cut(s) 775, 784
Bme18I GGWCC 5 cut(s) 330, 474, 525, 581, 1016
BmgT120I GGNCC 5 cut(s) 330, 474, 525, 581, 1016
BmiI GGNNCC 1 cut(s) 970
BmrFI CCNGG 2 cut(s) 775, 784
BmsI GCATC 1 cut(s) 506
BoxI GACNNNNGTC 2 cut(s) 283, 709
BpmI CTGGAG 2 cut(s) 319, 824
BpuEI CTTGAG 1 cut(s) 1119
BpuMI CCSGG 1 cut(s) 775
BsaJI CCNNGG 5 cut(s) 380, 687, 783, 852, 1066
BsaXI ACNNNNNCTCC 2 cut(s) 802, 832
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse1I ACTGG 1 cut(s) 71
BseBI CCWGG 1 cut(s) 784
BseDI CCNNGG 5 cut(s) 380, 687, 783, 852, 1066
BseGI GGATG 1 cut(s) 727
BseLI CCNNNNNNNGG 1 cut(s) 392
BseMII CTCAG 2 cut(s) 492, 635
BseNI ACTGG 1 cut(s) 71
BseRI GAGGAG 1 cut(s) 453
BseXI GCAGC 1 cut(s) 1008
BsgI GTGCAG 1 cut(s) 248
BshFI GGCC 2 cut(s) 166, 385
BsiSI CCGG 1 cut(s) 775
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 3 cut(s) 315, 317, 1165
BsnI GGCC 2 cut(s) 166, 385
Bsp143I GATC 4 cut(s) 156, 624, 938, 1148
BspACI CCGC 1 cut(s) 1019
BspANI GGCC 2 cut(s) 166, 385
BspCNI CTCAG 2 cut(s) 491, 634
BspLI GGNNCC 1 cut(s) 970
BspPI GGATC 2 cut(s) 946, 1156
BspTI CTTAAG 1 cut(s) 203
BsrI ACTGG 1 cut(s) 71
BssECI CCNNGG 5 cut(s) 380, 687, 783, 852, 1066
BssMI GATC 4 cut(s) 156, 624, 938, 1148
BssNAI GTATAC 1 cut(s) 260
BssT1I CCWWGG 3 cut(s) 380, 687, 852
Bst1107I GTATAC 1 cut(s) 260
Bst2UI CCWGG 1 cut(s) 784
Bst4CI ACNGT 2 cut(s) 282, 951
Bst6I CTCTTC 1 cut(s) 1117
BstAFI CTTAAG 1 cut(s) 203
BstC8I GCNNGC 3 cut(s) 560, 611, 1095
BstDEI CTNAG 4 cut(s) 478, 501, 507, 621
BstDSI CCRYGG 1 cut(s) 1066
BstF5I GGATG 1 cut(s) 727
BstKTI GATC 4 cut(s) 159, 627, 941, 1151
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 4 cut(s) 156, 624, 938, 1148
BstMWI GCNNNNNNNGC 2 cut(s) 52, 363
BstNI CCWGG 1 cut(s) 784
BstNSI RCATGY 1 cut(s) 27
BstPAI GACNNNNGTC 2 cut(s) 283, 709
BstSCI CCNGG 2 cut(s) 773, 782
BstSFI CTRYAG 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 1008
BstX2I RGATCY 2 cut(s) 938, 1148
BstYI RGATCY 2 cut(s) 938, 1148
BstZ17I GTATAC 1 cut(s) 260
BsuRI GGCC 2 cut(s) 166, 385
BtgI CCRYGG 1 cut(s) 1066
BtsCI GGATG 1 cut(s) 727
BtsI GCAGTG 1 cut(s) 236
BtsIMutI CAGTG 2 cut(s) 236, 634
Cac8I GCNNGC 3 cut(s) 560, 611, 1095
Cfr13I GGNCC 5 cut(s) 330, 474, 525, 581, 1016
CviAII CATG 3 cut(s) 24, 47, 588
DdeI CTNAG 4 cut(s) 478, 501, 507, 621
DpnI GATC 4 cut(s) 158, 626, 940, 1150
DpnII GATC 4 cut(s) 156, 624, 938, 1148
DraI TTTAAA 3 cut(s) 128, 535, 569
DraIII CACNNNGTG 2 cut(s) 590, 777
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
Eam1104I CTCTTC 1 cut(s) 1117
EarI CTCTTC 1 cut(s) 1117
Eco130I CCWWGG 3 cut(s) 380, 687, 852
Eco147I AGGCCT 1 cut(s) 385
Eco32I GATATC 2 cut(s) 200, 554
Eco47I GGWCC 5 cut(s) 330, 474, 525, 581, 1016
Eco57I CTGAAG 1 cut(s) 893
EcoO109I RGGNCCY 1 cut(s) 474
EcoRII CCWGG 1 cut(s) 782
EcoRV GATATC 2 cut(s) 200, 554
EcoT14I CCWWGG 3 cut(s) 380, 687, 852
EcoT22I ATGCAT 1 cut(s) 317
ErhI CCWWGG 3 cut(s) 380, 687, 852
FaeI CATG 3 cut(s) 27, 50, 591
FalI AAGNNNNNCTT 4 cut(s) 172, 204, 484, 516
FaqI GGGAC 1 cut(s) 77
FatI CATG 3 cut(s) 23, 46, 587
FauNDI CATATG 1 cut(s) 57
FblI GTMKAC 2 cut(s) 259, 820
Fnu4HI GCNGC 1 cut(s) 1022
FokI GGATG 1 cut(s) 734
Fsp4HI GCNGC 1 cut(s) 1022
FspBI CTAG 1 cut(s) 603
GluI GCNGC 1 cut(s) 1022
GsuI CTGGAG 2 cut(s) 319, 824
HaeIII GGCC 2 cut(s) 166, 385
HapII CCGG 1 cut(s) 775
Hin1II CATG 3 cut(s) 27, 50, 591
HincII GTYRAC 1 cut(s) 821
HindII GTYRAC 1 cut(s) 821
HindIII AAGCTT 1 cut(s) 607
HinfI GANTC 7 cut(s) 210, 244, 768, 799, 885, 916, 985
HpaII CCGG 1 cut(s) 775
HphI GGTGA 2 cut(s) 763, 789
Hpy166II GTNNAC 4 cut(s) 260, 584, 821, 1016
Hpy188I TCNGA 6 cut(s) 15, 197, 481, 624, 947, 1112
Hpy188III TCNNGA 7 cut(s) 336, 348, 549, 765, 803, 972, 1156
Hpy8I GTNNAC 4 cut(s) 260, 584, 821, 1016
HpyAV CCTTC 3 cut(s) 215, 396, 999
HpyCH4III ACNGT 2 cut(s) 282, 951
HpyCH4IV ACGT 1 cut(s) 362
HpyCH4V TGCA 5 cut(s) 179, 229, 315, 642, 1165
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 363
HpyF3I CTNAG 4 cut(s) 478, 501, 507, 621
HpySE526I ACGT 1 cut(s) 362
Hsp92II CATG 3 cut(s) 27, 50, 591
Kzo9I GATC 4 cut(s) 156, 624, 938, 1148
Lsp1109I GCAGC 1 cut(s) 1008
LweI GCATC 1 cut(s) 506
MaeI CTAG 1 cut(s) 603
MaeII ACGT 1 cut(s) 362
MaeIII GTNAC 4 cut(s) 207, 769, 993, 1001
MalI GATC 4 cut(s) 158, 626, 940, 1150
MboI GATC 4 cut(s) 156, 624, 938, 1148
MboII GAAGA 3 cut(s) 351, 750, 1134
MfeI CAATTG 2 cut(s) 88, 174
MflI RGATCY 2 cut(s) 938, 1148
MlyI GAGTC 5 cut(s) 204, 253, 777, 808, 979
MmeI TCCRAC 3 cut(s) 424, 916, 962
MnlI CCTC 9 cut(s) 257, 343, 431, 465, 487, 572, 630, 959, 1094
Mph1103I ATGCAT 1 cut(s) 317
MroXI GAANNNNTTC 2 cut(s) 315, 544
MseI TTAA 7 cut(s) 106, 127, 204, 534, 568, 663, 873
MslI CAYNNNNRTG 1 cut(s) 28
MspA1I CMGCKG 1 cut(s) 1021
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 1 cut(s) 775
MspR9I CCNGG 2 cut(s) 775, 784
MunI CAATTG 2 cut(s) 88, 174
Mva1269I GAATGC 3 cut(s) 315, 317, 1165
MvaI CCWGG 1 cut(s) 784
MwoI GCNNNNNNNGC 2 cut(s) 52, 363
NciI CCSGG 1 cut(s) 775
NdeI CATATG 1 cut(s) 57
NdeII GATC 4 cut(s) 156, 624, 938, 1148
NlaIII CATG 3 cut(s) 27, 50, 591
NlaIV GGNNCC 1 cut(s) 970
NmuCI GTSAC 2 cut(s) 207, 769
NsiI ATGCAT 1 cut(s) 317
NspI RCATGY 1 cut(s) 27
PceI AGGCCT 1 cut(s) 385
PciI ACATGT 1 cut(s) 23
PctI GAATGC 3 cut(s) 315, 317, 1165
PdmI GAANNNNTTC 2 cut(s) 315, 544
PfeI GAWTC 2 cut(s) 885, 916
PkrI GCNGC 1 cut(s) 1023
PleI GAGTC 5 cut(s) 204, 252, 776, 807, 979
PpsI GAGTC 5 cut(s) 204, 252, 776, 807, 979
PpuMI RGGWCCY 1 cut(s) 474
PscI ACATGT 1 cut(s) 23
PshAI GACNNNNGTC 2 cut(s) 283, 709
Psp5II RGGWCCY 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 782
PspGI CCWGG 1 cut(s) 782
PspN4I GGNNCC 1 cut(s) 970
PspPI GGNCC 5 cut(s) 330, 474, 525, 581, 1016
PspPPI RGGWCCY 1 cut(s) 474
PsuI RGATCY 2 cut(s) 938, 1148
RseI CAYNNNNRTG 1 cut(s) 28
SalI GTCGAC 1 cut(s) 819
SaqAI TTAA 7 cut(s) 106, 127, 204, 534, 568, 663, 873
SatI GCNGC 1 cut(s) 1022
Sau3AI GATC 4 cut(s) 156, 624, 938, 1148
Sau96I GGNCC 5 cut(s) 330, 474, 525, 581, 1016
SchI GAGTC 5 cut(s) 204, 253, 777, 808, 979
ScrFI CCNGG 2 cut(s) 775, 784
SfaNI GCATC 1 cut(s) 506
SfcI CTRYAG 1 cut(s) 281
SinI GGWCC 5 cut(s) 330, 474, 525, 581, 1016
SmiMI CAYNNNNRTG 1 cut(s) 28
SmlI CTYRAG 2 cut(s) 203, 1098
SmoI CTYRAG 2 cut(s) 203, 1098
SseBI AGGCCT 1 cut(s) 385
SsiI CCGC 1 cut(s) 1019
SspI AATATT 1 cut(s) 38
SspMI CTAG 1 cut(s) 603
StuI AGGCCT 1 cut(s) 385
StyD4I CCNGG 2 cut(s) 773, 782
StyI CCWWGG 3 cut(s) 380, 687, 852
TaaI ACNGT 2 cut(s) 282, 951
TaiI ACGT 1 cut(s) 365
TaqI TCGA 2 cut(s) 287, 820
TfiI GAWTC 2 cut(s) 885, 916
Tru1I TTAA 7 cut(s) 106, 127, 204, 534, 568, 663, 873
Tru9I TTAA 7 cut(s) 106, 127, 204, 534, 568, 663, 873
TscAI CASTG 2 cut(s) 236, 634
TseFI GTSAC 2 cut(s) 207, 769
TseI GCWGC 1 cut(s) 1021
Tsp45I GTSAC 2 cut(s) 207, 769
TspDTI ATGAA 5 cut(s) 46, 268, 738, 882, 1123
TspGWI ACGGA 2 cut(s) 498, 1055
TspRI CASTG 2 cut(s) 236, 634
Vha464I CTTAAG 1 cut(s) 203
VpaK11BI GGWCC 5 cut(s) 330, 474, 525, 581, 1016
XapI RAATTY 4 cut(s) 845, 869, 1104, 1142
XceI RCATGY 1 cut(s) 27
XmiI GTMKAC 2 cut(s) 259, 820
XmnI GAANNNNTTC 2 cut(s) 315, 544
XspI CTAG 1 cut(s) 603
Zsp2I ATGCAT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.