Rmu_sc0002016.1_g000015

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002016.1
OG0031179
Physical Location & Seq
Reverse (-)
51384 .. 52456
1073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002016.1_g000015.1.cds

Sequence Viewer

Length: 528 bp
atggaggaggccagggaaacactacaatctcatctatcatggtctgtatttgtacttgggctgggccctgatcttggttttgggcctggttggcgtgtcttgggagtttctttgccaaccggtggcggcaagtctgcaacaaccgatggtgcagtggtcatttggtctcattgggcccataggattggttttcggcttttcttcatgctactttgggcgtcgtggacagagcgcaataatgcagtttggaatggcaacggcatgattaatgttgacattaaaattgattgtgttgttgttgttactgcactcaatggagaaatagaggacctatcagaggtgggctatattgtagaggattgtaaatcttatctacaggatgttacttcttttcgattacaatccatttaccatgaagcaaatgatgtaacccatagattggcgcattttgctagtttatctgttttagatgagtattggttaaaggtagagctgggcacgagccgggacgggccggttgctgactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

175

Amino Acids

19.45

Weight (kDa)

4.91

Isoelectric Point (pI)

23.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031179)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002016.1_g000015
rosa_rugosa Rorug05G0536200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 122, 439
AciI CCGC 1 cut(s) 126
AcyI GRCGYC 1 cut(s) 218
AfaI GTAC 1 cut(s) 54
AfiI CCNNNNNNNGG 4 cut(s) 74, 122, 337, 439
AgeI ACCGGT 1 cut(s) 119
AjnI CCWGG 2 cut(s) 11, 85
AluBI AGCT 1 cut(s) 493
AluI AGCT 1 cut(s) 493
Alw26I GTCTC 1 cut(s) 171
AoxI GGCC 5 cut(s) 9, 64, 83, 174, 512
ApaI GGGCCC 2 cut(s) 68, 178
AseI ATTAAT 1 cut(s) 267
AsiGI ACCGGT 1 cut(s) 119
AspLEI GCGC 2 cut(s) 234, 445
AspS9I GGNCC 7 cut(s) 64, 65, 83, 174, 175, 328, 512
AsuC2I CCSGG 1 cut(s) 506
AvaII GGWCC 1 cut(s) 328
BaeGI GKGCMC 3 cut(s) 68, 178, 500
BanII GRGCYC 2 cut(s) 68, 178
BauI CACGAG 1 cut(s) 499
BccI CCATC 1 cut(s) 140
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 2 cut(s) 13, 87
BcnI CCSGG 1 cut(s) 506
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 453
BfmI CTRYAG 1 cut(s) 374
BglI GCCNNNNNGGC 1 cut(s) 91
BisI GCNGC 1 cut(s) 127
BlsI GCNGC 1 cut(s) 128
Bme1390I CCNGG 3 cut(s) 13, 87, 506
Bme18I GGWCC 1 cut(s) 328
BmgT120I GGNCC 7 cut(s) 64, 65, 83, 174, 175, 328, 512
BmiI GGNNCC 2 cut(s) 66, 176
BmrFI CCNGG 3 cut(s) 13, 87, 506
BpuMI CCSGG 1 cut(s) 506
BsaBI GATNNNNATC 1 cut(s) 400
BsaHI GRCGYC 1 cut(s) 218
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 1 cut(s) 119
Bsc4I CCNNNNNNNGG 4 cut(s) 74, 122, 337, 439
Bse118I RCCGGY 2 cut(s) 119, 514
Bse8I GATNNNNATC 1 cut(s) 400
BseBI CCWGG 2 cut(s) 13, 87
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 1 cut(s) 385
BseJI GATNNNNATC 1 cut(s) 400
BseLI CCNNNNNNNGG 4 cut(s) 74, 122, 337, 439
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 3 cut(s) 68, 178, 500
BseYI CCCAGC 2 cut(s) 61, 493
BsgI GTGCAG 2 cut(s) 171, 291
BshFI GGCC 5 cut(s) 11, 66, 85, 176, 514
BshTI ACCGGT 1 cut(s) 119
BsiSI CCGG 3 cut(s) 120, 505, 515
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 4 cut(s) 74, 122, 337, 439
BsmAI GTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 521
BsnI GGCC 5 cut(s) 11, 66, 85, 176, 514
Bso31I GGTCTC 1 cut(s) 171
Bsp120I GGGCCC 2 cut(s) 64, 174
Bsp1286I GDGCHC 3 cut(s) 68, 178, 500
Bsp143I GATC 1 cut(s) 70
BspACI CCGC 1 cut(s) 126
BspANI GGCC 5 cut(s) 11, 66, 85, 176, 514
BspLI GGNNCC 2 cut(s) 66, 176
BspTNI GGTCTC 1 cut(s) 171
BsrFI RCCGGY 2 cut(s) 119, 514
BssAI RCCGGY 2 cut(s) 119, 514
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 1 cut(s) 70
BssNI GRCGYC 1 cut(s) 218
BssSI CACGAG 1 cut(s) 499
Bst2BI CACGAG 1 cut(s) 499
Bst2UI CCWGG 2 cut(s) 13, 87
BstACI GRCGYC 1 cut(s) 218
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 1 cut(s) 385
BstHHI GCGC 2 cut(s) 234, 445
BstKTI GATC 1 cut(s) 73
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 1 cut(s) 70
BstMWI GCNNNNNNNGC 2 cut(s) 91, 449
BstNI CCWGG 2 cut(s) 13, 87
BstSCI CCNGG 3 cut(s) 11, 85, 504
BstSFI CTRYAG 1 cut(s) 374
BstSLI GKGCMC 3 cut(s) 68, 178, 500
BstXI CCANNNNNNTGG 1 cut(s) 185
BsuRI GGCC 5 cut(s) 11, 66, 85, 176, 514
BtsCI GGATG 1 cut(s) 385
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 1 cut(s) 159
CfoI GCGC 2 cut(s) 234, 445
Cfr10I RCCGGY 2 cut(s) 119, 514
Cfr13I GGNCC 7 cut(s) 64, 65, 83, 174, 175, 328, 512
CseI GACGC 1 cut(s) 207
Csp6I GTAC 1 cut(s) 53
CspAI ACCGGT 1 cut(s) 119
CviAII CATG 4 cut(s) 39, 205, 262, 413
CviQI GTAC 1 cut(s) 53
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
Eco24I GRGCYC 2 cut(s) 68, 178
Eco31I GGTCTC 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 328
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoO109I RGGNCCY 2 cut(s) 65, 328
EcoRII CCWGG 2 cut(s) 11, 85
EcoT38I GRGCYC 2 cut(s) 68, 178
FaeI CATG 4 cut(s) 42, 208, 265, 416
FaiI YATR 7 cut(s) 40, 180, 206, 263, 348, 414, 435
FaqI GGGAC 1 cut(s) 521
FatI CATG 4 cut(s) 38, 204, 261, 412
Fnu4HI GCNGC 1 cut(s) 127
FokI GGATG 1 cut(s) 392
FriOI GRGCYC 2 cut(s) 68, 178
Fsp4HI GCNGC 1 cut(s) 127
FspBI CTAG 1 cut(s) 453
GlaI GCGC 2 cut(s) 233, 444
GluI GCNGC 1 cut(s) 127
GsaI CCCAGC 2 cut(s) 65, 497
HaeIII GGCC 5 cut(s) 11, 66, 85, 176, 514
HapII CCGG 3 cut(s) 120, 505, 515
HgaI GACGC 1 cut(s) 207
HhaI GCGC 2 cut(s) 234, 445
Hin1I GRCGYC 1 cut(s) 218
Hin1II CATG 4 cut(s) 42, 208, 265, 416
Hin6I GCGC 2 cut(s) 232, 443
HinP1I GCGC 2 cut(s) 232, 443
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HpaII CCGG 3 cut(s) 120, 505, 515
Hpy166II GTNNAC 2 cut(s) 225, 274
Hpy188I TCNGA 1 cut(s) 337
Hpy8I GTNNAC 2 cut(s) 225, 274
Hpy99I CGWCG 1 cut(s) 223
HpyCH4V TGCA 4 cut(s) 137, 152, 242, 308
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 449
Hsp92I GRCGYC 1 cut(s) 218
Hsp92II CATG 4 cut(s) 42, 208, 265, 416
HspAI GCGC 2 cut(s) 232, 443
Kzo9I GATC 1 cut(s) 70
LpnPI CCDG 9 cut(s) 25, 47, 72, 81, 99, 133, 362, 479, 518
MaeI CTAG 1 cut(s) 453
MaeIII GTNAC 3 cut(s) 301, 382, 427
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MboII GAAGA 1 cut(s) 193
MhlI GDGCHC 3 cut(s) 68, 178, 500
MluCI AATT 1 cut(s) 282
MnlI CCTC 3 cut(s) 319, 331, 349
MseI TTAA 3 cut(s) 267, 279, 482
MspI CCGG 3 cut(s) 120, 505, 515
MspR9I CCNGG 3 cut(s) 13, 87, 506
MvaI CCWGG 2 cut(s) 13, 87
MwoI GCNNNNNNNGC 2 cut(s) 91, 449
NciI CCSGG 1 cut(s) 506
NdeII GATC 1 cut(s) 70
NlaIII CATG 4 cut(s) 42, 208, 265, 416
NlaIV GGNNCC 2 cut(s) 66, 176
PflMI CCANNNNNTGG 2 cut(s) 122, 439
PinAI ACCGGT 1 cut(s) 119
PkrI GCNGC 1 cut(s) 128
PpuMI RGGWCCY 1 cut(s) 328
PshBI ATTAAT 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 2 cut(s) 11, 85
PspFI CCCAGC 2 cut(s) 61, 493
PspGI CCWGG 2 cut(s) 11, 85
PspN4I GGNNCC 2 cut(s) 66, 176
PspOMI GGGCCC 2 cut(s) 64, 174
PspPI GGNCC 7 cut(s) 64, 65, 83, 174, 175, 328, 512
PspPPI RGGWCCY 1 cut(s) 328
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
SaqAI TTAA 3 cut(s) 267, 279, 482
SatI GCNGC 1 cut(s) 127
Sau3AI GATC 1 cut(s) 70
Sau96I GGNCC 7 cut(s) 64, 65, 83, 174, 175, 328, 512
ScrFI CCNGG 3 cut(s) 13, 87, 506
SduI GDGCHC 3 cut(s) 68, 178, 500
SetI ASST 4 cut(s) 333, 342, 489, 495
SfcI CTRYAG 1 cut(s) 374
SinI GGWCC 1 cut(s) 328
Sse9I AATT 1 cut(s) 282
SsiI CCGC 1 cut(s) 126
SspMI CTAG 1 cut(s) 453
StyD4I CCNGG 3 cut(s) 11, 85, 504
TaqI TCGA 1 cut(s) 394
TasI AATT 1 cut(s) 282
TatI WGTACW 1 cut(s) 52
TauI GCSGC 1 cut(s) 129
Tru1I TTAA 3 cut(s) 267, 279, 482
Tru9I TTAA 3 cut(s) 267, 279, 482
TscAI CASTG 1 cut(s) 159
TspDTI ATGAA 2 cut(s) 193, 429
TspRI CASTG 1 cut(s) 159
Van91I CCANNNNNTGG 2 cut(s) 122, 439
VpaK11BI GGWCC 1 cut(s) 328
VspI ATTAAT 1 cut(s) 267
XagI CCTNNNNNAGG 1 cut(s) 335
XspI CTAG 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.