Rmu_sc0002028.1_g000004

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002028.1
OG0023077
Physical Location & Seq
Forward (+)
25006 .. 26315
1310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002028.1_g000004.1.cds

Sequence Viewer

Length: 873 bp
atgttgggttgtttccgcttctttaccgaagtttggttgattctctctcgcttactttgcttgatggaggaagttgtcgatagtttttccaggaaattggctttatcggaggcggaagtcaagcaggagtcagatcttggttggatcttgaggggcagtccatggtcctttgagaaggctttggttgctgtagcggttacaaatggcactgaagatccttgcacggtttcattagacactcaatatttctggattcgcatccggggtttacctccactccatatggatgatgaaatggtggttaatacaggccggtgtattggccaggcaattggtctttttgtgagggcagaaaaggctgtggagttagtttttgggggatttcttgcgtatcagagtagggatcaatgtcaaggcttctctgaagaagtgtgtgaaggcttgaggcattatgctacaaaaaaaaagaatgggtggatcatgcaggcccctaatttccctgtggtgggcttcaccaggactagagatgagcgtgatgaggatgctgttgattgcattggagaaggggagtccatggcattggattcggggggtgaagtggaggatacagcaggaggtcaattggcgaaaagaagatgggttttgagtgagttttctgagctttctactggtactggggaggggcttaacacccagccatgtctgaggcacgacccggtactggtcaacccatccttgtcaacatctttgaagagtgtttcaaatgttgacgtttgttttggaaaggagtccaaatccattaaggagtcgaagtcatctgcaactcttagaagtaacaaagaaaatttaaatgtctatcagagaagggtctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1

Protein Analysis

290

Amino Acids

32.28

Weight (kDa)

5.15

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023077)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002028.1_g000004
rosa_samantha Rh5AG358100 Rh5BG370400 Rh5DG384000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 16, 113, 194
AclWI GGATC 4 cut(s) 152, 209, 411, 485
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 1 cut(s) 844
AcuI CTGAAG 2 cut(s) 231, 444
AfaI GTAC 2 cut(s) 673, 720
AfiI CCNNNNNNNGG 4 cut(s) 33, 505, 506, 721
AgsI TTSAA 2 cut(s) 751, 762
AjnI CCWGG 3 cut(s) 89, 324, 515
AluBI AGCT 1 cut(s) 661
AluI AGCT 1 cut(s) 661
AlwI GGATC 4 cut(s) 152, 209, 411, 485
AoxI GGCC 3 cut(s) 310, 322, 486
ApoI RAATTY 1 cut(s) 844
ArsI GACNNNNNNTTYG 2 cut(s) 761, 793
AspS9I GGNCC 2 cut(s) 165, 487
AsuC2I CCSGG 2 cut(s) 263, 716
AsuHPI GGTGA 2 cut(s) 505, 605
AvaII GGWCC 1 cut(s) 165
BalI TGGCCA 1 cut(s) 324
BccI CCATC 3 cut(s) 58, 630, 739
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BciT130I CCWGG 3 cut(s) 91, 326, 517
BciVI GTATCC 1 cut(s) 598
BcnI CCSGG 2 cut(s) 263, 716
BfaI CTAG 2 cut(s) 522, 871
BfmI CTRYAG 1 cut(s) 189
BfuI GTATCC 1 cut(s) 598
BglII AGATCT 1 cut(s) 133
Bme1390I CCNGG 5 cut(s) 91, 263, 326, 517, 716
Bme18I GGWCC 1 cut(s) 165
BmgT120I GGNCC 2 cut(s) 165, 487
BmiI GGNNCC 1 cut(s) 489
BmrFI CCNGG 5 cut(s) 91, 263, 326, 517, 716
BmrI ACTGGG 1 cut(s) 684
BmsI GCATC 2 cut(s) 267, 532
BmuI ACTGGG 1 cut(s) 684
BpuEI CTTGAG 2 cut(s) 169, 463
BpuMI CCSGG 2 cut(s) 263, 716
BsaBI GATNNNNATC 1 cut(s) 257
BsaJI CCNNGG 3 cut(s) 161, 262, 573
BsaXI ACNNNNNCTCC 4 cut(s) 261, 291, 797, 827
Bsc4I CCNNNNNNNGG 4 cut(s) 33, 505, 506, 721
Bse118I RCCGGY 1 cut(s) 312
Bse1I ACTGG 3 cut(s) 673, 679, 726
Bse8I GATNNNNATC 1 cut(s) 257
BseBI CCWGG 3 cut(s) 91, 326, 517
BseDI CCNNGG 3 cut(s) 161, 262, 573
BseGI GGATG 4 cut(s) 258, 292, 547, 731
BseJI GATNNNNATC 1 cut(s) 257
BseLI CCNNNNNNNGG 4 cut(s) 33, 505, 506, 721
BseMII CTCAG 2 cut(s) 648, 695
BseNI ACTGG 3 cut(s) 673, 679, 726
BseYI CCCAGC 1 cut(s) 693
BshFI GGCC 3 cut(s) 312, 324, 488
BsiSI CCGG 3 cut(s) 262, 313, 716
BslI CCNNNNNNNGG 4 cut(s) 33, 505, 506, 721
BsnI GGCC 3 cut(s) 312, 324, 488
Bsp143I GATC 5 cut(s) 133, 144, 214, 403, 477
Bsp19I CCATGG 2 cut(s) 161, 573
BspACI CCGC 3 cut(s) 16, 113, 194
BspANI GGCC 3 cut(s) 312, 324, 488
BspCNI CTCAG 2 cut(s) 649, 696
BspLI GGNNCC 1 cut(s) 489
BspPI GGATC 4 cut(s) 152, 209, 411, 485
BsrFI RCCGGY 1 cut(s) 312
BsrI ACTGG 3 cut(s) 673, 679, 726
BssAI RCCGGY 1 cut(s) 312
BssECI CCNNGG 3 cut(s) 161, 262, 573
BssMI GATC 5 cut(s) 133, 144, 214, 403, 477
BssT1I CCWWGG 2 cut(s) 161, 573
Bst2UI CCWGG 3 cut(s) 91, 326, 517
Bst4CI ACNGT 1 cut(s) 226
Bst6I CTCTTC 1 cut(s) 746
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 3 cut(s) 657, 704, 827
BstDSI CCRYGG 2 cut(s) 161, 573
BstF5I GGATG 4 cut(s) 258, 292, 547, 731
BstKTI GATC 5 cut(s) 136, 147, 217, 406, 480
BstMBI GATC 5 cut(s) 133, 144, 214, 403, 477
BstMWI GCNNNNNNNGC 3 cut(s) 57, 185, 356
BstNI CCWGG 3 cut(s) 91, 326, 517
BstSCI CCNGG 5 cut(s) 89, 261, 324, 515, 714
BstSFI CTRYAG 1 cut(s) 189
BstX2I RGATCY 3 cut(s) 133, 144, 214
BstXI CCANNNNNNTGG 3 cut(s) 97, 332, 580
BstYI RGATCY 3 cut(s) 133, 144, 214
BsuI GTATCC 1 cut(s) 598
BsuRI GGCC 3 cut(s) 312, 324, 488
BtgI CCRYGG 2 cut(s) 161, 573
BtsCI GGATG 4 cut(s) 258, 292, 547, 731
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 1 cut(s) 486
Cfr10I RCCGGY 1 cut(s) 312
Cfr13I GGNCC 2 cut(s) 165, 487
Csp6I GTAC 2 cut(s) 672, 719
CviAII CATG 4 cut(s) 162, 481, 574, 699
CviQI GTAC 2 cut(s) 672, 719
DdeI CTNAG 3 cut(s) 657, 704, 827
DpnI GATC 5 cut(s) 135, 146, 216, 405, 479
DpnII GATC 5 cut(s) 133, 144, 214, 403, 477
DraI TTTAAA 1 cut(s) 849
EaeI YGGCCR 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 746
EarI CTCTTC 1 cut(s) 746
EciI GGCGGA 1 cut(s) 128
Eco130I CCWWGG 2 cut(s) 161, 573
Eco47I GGWCC 1 cut(s) 165
Eco57I CTGAAG 2 cut(s) 231, 444
EcoO109I RGGNCCY 1 cut(s) 487
EcoRII CCWGG 3 cut(s) 89, 324, 515
EcoT14I CCWWGG 2 cut(s) 161, 573
ErhI CCWWGG 2 cut(s) 161, 573
FaeI CATG 4 cut(s) 165, 484, 577, 702
FaiI YATR 7 cut(s) 163, 282, 284, 453, 482, 575, 700
FatI CATG 4 cut(s) 161, 480, 573, 698
FauNDI CATATG 1 cut(s) 282
FokI GGATG 4 cut(s) 245, 299, 554, 718
FspBI CTAG 2 cut(s) 522, 871
GsaI CCCAGC 1 cut(s) 697
HaeIII GGCC 3 cut(s) 312, 324, 488
HapII CCGG 3 cut(s) 262, 313, 716
Hin1II CATG 4 cut(s) 165, 484, 577, 702
HincII GTYRAC 3 cut(s) 727, 741, 769
HindII GTYRAC 3 cut(s) 727, 741, 769
HinfI GANTC 7 cut(s) 40, 128, 253, 569, 584, 788, 806
HpaII CCGG 3 cut(s) 262, 313, 716
HphI GGTGA 2 cut(s) 505, 605
Hpy166II GTNNAC 4 cut(s) 269, 727, 741, 769
Hpy188I TCNGA 7 cut(s) 109, 133, 396, 424, 658, 705, 861
Hpy188III TCNNGA 2 cut(s) 148, 250
Hpy8I GTNNAC 4 cut(s) 269, 727, 741, 769
HpyAV CCTTC 4 cut(s) 169, 431, 557, 858
HpyCH4III ACNGT 1 cut(s) 226
HpyCH4IV ACGT 1 cut(s) 771
HpyCH4V TGCA 4 cut(s) 222, 484, 555, 821
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 185, 356
HpyF3I CTNAG 3 cut(s) 657, 704, 827
HpySE526I ACGT 1 cut(s) 771
Hsp92II CATG 4 cut(s) 165, 484, 577, 702
Kzo9I GATC 5 cut(s) 133, 144, 214, 403, 477
LweI GCATC 2 cut(s) 267, 532
MaeI CTAG 2 cut(s) 522, 871
MaeII ACGT 1 cut(s) 771
MaeIII GTNAC 2 cut(s) 196, 833
MalI GATC 5 cut(s) 135, 146, 216, 405, 479
MboI GATC 5 cut(s) 133, 144, 214, 403, 477
MboII GAAGA 4 cut(s) 224, 437, 645, 763
MfeI CAATTG 2 cut(s) 330, 620
MflI RGATCY 3 cut(s) 133, 144, 214
MlsI TGGCCA 1 cut(s) 324
MluCI AATT 5 cut(s) 95, 330, 493, 620, 844
MluNI TGGCCA 1 cut(s) 324
MlyI GAGTC 4 cut(s) 137, 578, 797, 815
MmeI TCCRAC 1 cut(s) 122
Mox20I TGGCCA 1 cut(s) 324
MscI TGGCCA 1 cut(s) 324
MseI TTAA 4 cut(s) 303, 687, 801, 848
MslI CAYNNNNRTG 1 cut(s) 285
Msp20I TGGCCA 1 cut(s) 324
MspI CCGG 3 cut(s) 262, 313, 716
MspR9I CCNGG 5 cut(s) 91, 263, 326, 517, 716
MunI CAATTG 2 cut(s) 330, 620
MvaI CCWGG 3 cut(s) 91, 326, 517
MwoI GCNNNNNNNGC 3 cut(s) 57, 185, 356
NciI CCSGG 2 cut(s) 263, 716
NcoI CCATGG 2 cut(s) 161, 573
NdeI CATATG 1 cut(s) 282
NdeII GATC 5 cut(s) 133, 144, 214, 403, 477
NlaIII CATG 4 cut(s) 165, 484, 577, 702
NlaIV GGNNCC 1 cut(s) 489
PfeI GAWTC 3 cut(s) 40, 253, 584
PfoI TCCNGGA 1 cut(s) 89
PleI GAGTC 4 cut(s) 136, 577, 796, 814
PpsI GAGTC 4 cut(s) 136, 577, 796, 814
Psp6I CCWGG 3 cut(s) 89, 324, 515
PspFI CCCAGC 1 cut(s) 693
PspGI CCWGG 3 cut(s) 89, 324, 515
PspN4I GGNNCC 1 cut(s) 489
PspPI GGNCC 2 cut(s) 165, 487
PsuI RGATCY 3 cut(s) 133, 144, 214
RsaI GTAC 2 cut(s) 673, 720
RsaNI GTAC 2 cut(s) 672, 719
RseI CAYNNNNRTG 1 cut(s) 285
SaqAI TTAA 4 cut(s) 303, 687, 801, 848
Sau3AI GATC 5 cut(s) 133, 144, 214, 403, 477
Sau96I GGNCC 2 cut(s) 165, 487
SchI GAGTC 4 cut(s) 137, 578, 797, 815
ScrFI CCNGG 5 cut(s) 91, 263, 326, 517, 716
SetI ASST 4 cut(s) 274, 619, 663, 774
SfaNI GCATC 2 cut(s) 267, 532
SfcI CTRYAG 1 cut(s) 189
SinI GGWCC 1 cut(s) 165
SmiI ATTTAAAT 1 cut(s) 849
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 2 cut(s) 148, 442
SmoI CTYRAG 2 cut(s) 148, 442
Sse9I AATT 5 cut(s) 95, 330, 493, 620, 844
SsiI CCGC 3 cut(s) 16, 113, 194
SspI AATATT 1 cut(s) 245
SspMI CTAG 2 cut(s) 522, 871
StyD4I CCNGG 5 cut(s) 89, 261, 324, 515, 714
StyI CCWWGG 2 cut(s) 161, 573
SwaI ATTTAAAT 1 cut(s) 849
TaaI ACNGT 1 cut(s) 226
TaiI ACGT 1 cut(s) 774
TaqI TCGA 2 cut(s) 78, 809
TasI AATT 5 cut(s) 95, 330, 493, 620, 844
TfiI GAWTC 3 cut(s) 40, 253, 584
Tru1I TTAA 4 cut(s) 303, 687, 801, 848
Tru9I TTAA 4 cut(s) 303, 687, 801, 848
TscAI CASTG 1 cut(s) 214
TspDTI ATGAA 2 cut(s) 219, 306
TspRI CASTG 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 165
XapI RAATTY 1 cut(s) 844
XspI CTAG 2 cut(s) 522, 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.