Rmu_sc0002312.1_g000029

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002312.1
OG0031202
Physical Location & Seq
Reverse (-)
139418 .. 141392
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002312.1_g000029.1.cds

Sequence Viewer

Length: 900 bp
atgggtgaatttgctctaacaacctcagagtgttttagtctgttggatatgaagtgttatggtggtaatgttgcagttaaggttgatattgccaaggcttttgacacacattcatgggatttgttctacatgttcttgaagcctttggatttcatctcaccatttttggttgggtgcatgtcattctacgaagtggttcttaatcgtggttttactgagcttgttatctctggtcggttgcatcttatttcttctccaagatgtatgaaagcgcattcccatgtgttatttgctgatgatgtaattgttttttaccgtggggaaaagcacaacttggctatgattgtcaatgataattcaatgtggctgattgactctggttctgaagtttctatttggagggataaattcttggagtggcctctcattgatttttttgggggacatgttggtgtgatggatgatttaaatggtacggtggtagattttgtatctaatggatcttggaatcttcctcctttattacaacttcacttcttaggtctttgtgacctcattgagccagttatcatcccttctaatcctttagctagtgatgaacttatttggtttgcatcatcgtcagttcattacacccggtgtgtctttgatgacatggcaggtcttacatggctatgttctttcggaggatctattgcaaatgagggaaacaaggttagatttgatggtgaagtggttcatgcaattaagatgtttgggtttattgttgggcatattcaagcttctaatcgccttgttactggaatcatgcagaactctattcttgatcttcgagccttgaagtttttttggacaagttgtaatcctcgcagagctcttagagttcttgaggttaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

299

Amino Acids

33.65

Weight (kDa)

5.4

Isoelectric Point (pI)

33.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031202)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002312.1_g000029 Rmu_sc0007785.1_g000032

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 650
AclWI GGATC 2 cut(s) 508, 697
AcsI RAATTY 2 cut(s) 8, 407
AcuI CTGAAG 1 cut(s) 405
AdeI CACNNNGTG 1 cut(s) 639
AfaI GTAC 1 cut(s) 475
AflIII ACRYGT 2 cut(s) 129, 445
AgsI TTSAA 4 cut(s) 139, 360, 779, 841
AluBI AGCT 4 cut(s) 220, 590, 782, 875
AluI AGCT 4 cut(s) 220, 590, 782, 875
Alw21I GWGCWC 1 cut(s) 877
AlwI GGATC 2 cut(s) 508, 697
AoxI GGCC 1 cut(s) 419
ApoI RAATTY 2 cut(s) 8, 407
Asp700I GAANNNNTTC 2 cut(s) 195, 735
AspLEI GCGC 1 cut(s) 274
AsuC2I CCSGG 1 cut(s) 637
AsuHPI GGTGA 3 cut(s) 17, 150, 740
BaeI ACNNNNGTAYC 2 cut(s) 465, 498
BanII GRGCYC 1 cut(s) 877
Bbv12I GWGCWC 1 cut(s) 877
BccI CCATC 2 cut(s) 451, 719
BcnI CCSGG 1 cut(s) 637
BfaI CTAG 1 cut(s) 591
BfuAI ACCTGC 1 cut(s) 650
Bme1390I CCNGG 1 cut(s) 637
BmrFI CCNGG 1 cut(s) 637
BmsI GCATC 2 cut(s) 250, 623
BpuMI CCSGG 1 cut(s) 637
BsaJI CCNNGG 2 cut(s) 93, 316
Bse1I ACTGG 2 cut(s) 563, 805
BseDI CCNNGG 2 cut(s) 93, 316
BseGI GGATG 2 cut(s) 466, 570
BseMII CTCAG 2 cut(s) 39, 207
BseNI ACTGG 2 cut(s) 563, 805
BshFI GGCC 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 877
BsiSI CCGG 1 cut(s) 637
BslFI GGGAC 1 cut(s) 456
BsmFI GGGAC 1 cut(s) 456
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 1 cut(s) 421
Bsp1286I GDGCHC 1 cut(s) 877
Bsp143I GATC 3 cut(s) 500, 689, 826
BspANI GGCC 1 cut(s) 421
BspCNI CTCAG 2 cut(s) 38, 208
BspMI ACCTGC 1 cut(s) 650
BspPI GGATC 2 cut(s) 508, 697
BsrI ACTGG 2 cut(s) 563, 805
BssECI CCNNGG 2 cut(s) 93, 316
BssMI GATC 3 cut(s) 500, 689, 826
BssT1I CCWWGG 1 cut(s) 93
Bst4CI ACNGT 2 cut(s) 317, 478
BstDEI CTNAG 4 cut(s) 25, 216, 538, 878
BstDSI CCRYGG 1 cut(s) 316
BstF5I GGATG 2 cut(s) 466, 570
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 3 cut(s) 503, 692, 829
BstMBI GATC 3 cut(s) 500, 689, 826
BstNSI RCATGY 3 cut(s) 133, 181, 449
BstSCI CCNGG 1 cut(s) 635
BstX2I RGATCY 2 cut(s) 500, 689
BstYI RGATCY 2 cut(s) 500, 689
BsuRI GGCC 1 cut(s) 421
BtgI CCRYGG 1 cut(s) 316
BtsCI GGATG 2 cut(s) 466, 570
BveI ACCTGC 1 cut(s) 650
CfoI GCGC 1 cut(s) 274
Csp6I GTAC 1 cut(s) 474
CviAII CATG 9 cut(s) 114, 130, 178, 281, 446, 655, 669, 740, 808
CviQI GTAC 1 cut(s) 474
DdeI CTNAG 4 cut(s) 25, 216, 538, 878
DpnI GATC 3 cut(s) 502, 691, 828
DpnII GATC 3 cut(s) 500, 689, 826
DraI TTTAAA 1 cut(s) 468
DraIII CACNNNGTG 1 cut(s) 639
Ecl136II GAGCTC 1 cut(s) 875
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 877
Eco53kI GAGCTC 1 cut(s) 875
Eco57I CTGAAG 1 cut(s) 405
EcoICRI GAGCTC 1 cut(s) 875
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 877
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 9 cut(s) 117, 133, 181, 284, 449, 658, 672, 743, 811
FalI AAGNNNNNCTT 4 cut(s) 183, 215, 317, 349
FaqI GGGAC 1 cut(s) 456
FatI CATG 9 cut(s) 113, 129, 177, 280, 445, 654, 668, 739, 807
FokI GGATG 2 cut(s) 473, 557
FriOI GRGCYC 1 cut(s) 877
FspBI CTAG 1 cut(s) 591
GlaI GCGC 1 cut(s) 273
HaeIII GGCC 1 cut(s) 421
HapII CCGG 1 cut(s) 637
HhaI GCGC 1 cut(s) 274
Hin1II CATG 9 cut(s) 117, 133, 181, 284, 449, 658, 672, 743, 811
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HindIII AAGCTT 1 cut(s) 780
HinfI GANTC 3 cut(s) 374, 508, 804
HpaII CCGG 1 cut(s) 637
HphI GGTGA 3 cut(s) 17, 150, 740
Hpy188I TCNGA 3 cut(s) 28, 385, 686
Hpy188III TCNNGA 3 cut(s) 136, 824, 887
HpyAV CCTTC 1 cut(s) 585
HpyCH4III ACNGT 2 cut(s) 317, 478
HpyCH4V TGCA 7 cut(s) 74, 177, 241, 614, 698, 743, 811
HpyF3I CTNAG 4 cut(s) 25, 216, 538, 878
Hsp92II CATG 9 cut(s) 117, 133, 181, 284, 449, 658, 672, 743, 811
HspAI GCGC 1 cut(s) 272
Kzo9I GATC 3 cut(s) 500, 689, 826
LpnPI CCDG 6 cut(s) 216, 363, 576, 645, 650, 786
LweI GCATC 2 cut(s) 250, 623
MaeI CTAG 1 cut(s) 591
MaeIII GTNAC 2 cut(s) 548, 796
MalI GATC 3 cut(s) 502, 691, 828
MboI GATC 3 cut(s) 500, 689, 826
MboII GAAGA 3 cut(s) 243, 503, 821
MflI RGATCY 2 cut(s) 500, 689
MhlI GDGCHC 1 cut(s) 877
MluCI AATT 6 cut(s) 8, 303, 355, 407, 744, 895
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 1 cut(s) 24
MnlI CCTC 9 cut(s) 34, 393, 432, 525, 563, 680, 697, 876, 883
MroXI GAANNNNTTC 2 cut(s) 195, 735
MseI TTAA 5 cut(s) 78, 201, 467, 747, 894
MslI CAYNNNNRTG 4 cut(s) 112, 279, 450, 673
MspI CCGG 1 cut(s) 637
MspR9I CCNGG 1 cut(s) 637
Mva1269I GAATGC 1 cut(s) 274
NciI CCSGG 1 cut(s) 637
NdeII GATC 3 cut(s) 500, 689, 826
NlaIII CATG 9 cut(s) 117, 133, 181, 284, 449, 658, 672, 743, 811
NmuCI GTSAC 1 cut(s) 548
NspI RCATGY 3 cut(s) 133, 181, 449
PciI ACATGT 2 cut(s) 129, 445
PctI GAATGC 1 cut(s) 274
PdmI GAANNNNTTC 2 cut(s) 195, 735
PfeI GAWTC 2 cut(s) 508, 804
PleI GAGTC 1 cut(s) 368
PpsI GAGTC 1 cut(s) 368
PscI ACATGT 2 cut(s) 129, 445
Psp124BI GAGCTC 1 cut(s) 877
PsuI RGATCY 2 cut(s) 500, 689
RsaI GTAC 1 cut(s) 475
RsaNI GTAC 1 cut(s) 474
RseI CAYNNNNRTG 4 cut(s) 112, 279, 450, 673
SacI GAGCTC 1 cut(s) 877
SaqAI TTAA 5 cut(s) 78, 201, 467, 747, 894
Sau3AI GATC 3 cut(s) 500, 689, 826
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 1 cut(s) 637
SduI GDGCHC 1 cut(s) 877
SfaNI GCATC 2 cut(s) 250, 623
SmiI ATTTAAAT 1 cut(s) 468
SmiMI CAYNNNNRTG 4 cut(s) 112, 279, 450, 673
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
Sse9I AATT 6 cut(s) 8, 303, 355, 407, 744, 895
SspMI CTAG 1 cut(s) 591
SstI GAGCTC 1 cut(s) 877
StyD4I CCNGG 1 cut(s) 635
StyI CCWWGG 1 cut(s) 93
SwaI ATTTAAAT 1 cut(s) 468
TaaI ACNGT 2 cut(s) 317, 478
TaqI TCGA 1 cut(s) 832
TasI AATT 6 cut(s) 8, 303, 355, 407, 744, 895
TfiI GAWTC 2 cut(s) 508, 804
Tru1I TTAA 5 cut(s) 78, 201, 467, 747, 894
Tru9I TTAA 5 cut(s) 78, 201, 467, 747, 894
TseFI GTSAC 1 cut(s) 548
Tsp45I GTSAC 1 cut(s) 548
TspDTI ATGAA 7 cut(s) 65, 102, 142, 281, 612, 617, 728
XapI RAATTY 2 cut(s) 8, 407
XceI RCATGY 3 cut(s) 133, 181, 449
XmnI GAANNNNTTC 2 cut(s) 195, 735
XspI CTAG 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.