Rmu_sc0007785.1_g000032

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007785.1
OG0031202
Physical Location & Seq
Reverse (-)
106251 .. 108314
2064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007785.1_g000032.1.cds

Sequence Viewer

Length: 993 bp
atgaggattacaataccatccccaatcttagataacggggattggggatccccatccccattccccatttgtccccgaattctccccccattaggggcgggtatccaatggagctctgccacgctggggatttttgtcatccctaagtgttatggtggtaatgttgcaattaaggttgatattgccaaggcttttgacacactttcatgggatttgttctatatgttcttgaagcctttggttttcatctcaccgtttttggttgggtgcatgtcattctacgaagtggttcttaatcgtggttttactgagcttgttaactctggtcggttgcatcttatttcttctccaagatgtataaaagcgccttcccatgtgttatttgctgatgatgtaattgttttttaccgtggggaaaagcacaacttggctgtgattgtcaatgataattcaatgtggctgattgactctggttctgaagtttctatttggagggataaattcttggagtggcctctcattgatttttttgggggacatgttggtgtgatggatgatttaaatggtacggtggtagattttgtatctaatggatcttggaatcttcctcctttattacaacttcacttcttaggtctttgtgacctcattgagccagttatcatcgcttctaatcctttagctagtgatgaacttatttgttttgcatcatcgtcagttcattacacccggtgtgtctttgatgacatggcaggtcttacatggctatgttctttcggaggatctattgcaaatgagggaaacaaggttagatttgatggtgaagtggttcatgtaattaagatgtttgggtttattgttgcgcatattcaagcttctaatcgccttgttactggattcatgcagaactctattcttgatcttcgagccttgaagtgtttttggacaagttgttatcctcgtagagctcttagagttcttgaggttaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

330

Amino Acids

36.71

Weight (kDa)

5.73

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031202)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002312.1_g000029 Rmu_sc0007785.1_g000032

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 864
Acc36I ACCTGC 1 cut(s) 743
AciI CCGC 1 cut(s) 98
AclWI GGATC 4 cut(s) 42, 55, 601, 790
AcsI RAATTY 2 cut(s) 78, 500
AcuI CTGAAG 1 cut(s) 498
AdeI CACNNNGTG 1 cut(s) 732
AfaI GTAC 1 cut(s) 568
AfiI CCNNNNNNNGG 4 cut(s) 92, 93, 94, 126
AflIII ACRYGT 1 cut(s) 538
AgsI TTSAA 4 cut(s) 232, 453, 872, 934
AluBI AGCT 5 cut(s) 114, 313, 683, 875, 968
AluI AGCT 5 cut(s) 114, 313, 683, 875, 968
Alw21I GWGCWC 2 cut(s) 116, 970
AlwI GGATC 4 cut(s) 42, 55, 601, 790
AoxI GGCC 1 cut(s) 512
ApoI RAATTY 2 cut(s) 78, 500
Asp700I GAANNNNTTC 2 cut(s) 288, 828
AspLEI GCGC 2 cut(s) 367, 865
AsuC2I CCSGG 1 cut(s) 730
AsuHPI GGTGA 2 cut(s) 243, 833
BaeI ACNNNNGTAYC 2 cut(s) 558, 591
BamHI GGATCC 1 cut(s) 47
BanII GRGCYC 2 cut(s) 116, 970
Bbv12I GWGCWC 2 cut(s) 116, 970
BccI CCATC 4 cut(s) 25, 61, 544, 812
BciVI GTATCC 1 cut(s) 113
BcnI CCSGG 1 cut(s) 730
BfaI CTAG 1 cut(s) 684
BfoI RGCGCY 1 cut(s) 368
BfuAI ACCTGC 1 cut(s) 743
BfuI GTATCC 1 cut(s) 113
Bme1390I CCNGG 1 cut(s) 730
BmiI GGNNCC 1 cut(s) 49
BmrFI CCNGG 1 cut(s) 730
BmsI GCATC 2 cut(s) 343, 716
BpuMI CCSGG 1 cut(s) 730
BsaBI GATNNNNATC 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 186, 409
Bsc4I CCNNNNNNNGG 4 cut(s) 92, 93, 94, 126
Bse1I ACTGG 2 cut(s) 656, 898
Bse8I GATNNNNATC 1 cut(s) 52
BseDI CCNNGG 2 cut(s) 186, 409
BseGI GGATG 4 cut(s) 17, 53, 138, 559
BseJI GATNNNNATC 1 cut(s) 52
BseLI CCNNNNNNNGG 4 cut(s) 92, 93, 94, 126
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 2 cut(s) 656, 898
BseYI CCCAGC 1 cut(s) 124
BshFI GGCC 1 cut(s) 514
BsiHKAI GWGCWC 2 cut(s) 116, 970
BsiSI CCGG 1 cut(s) 730
BslFI GGGAC 2 cut(s) 57, 549
BslI CCNNNNNNNGG 4 cut(s) 92, 93, 94, 126
BsmFI GGGAC 2 cut(s) 57, 549
BsnI GGCC 1 cut(s) 514
Bsp1286I GDGCHC 2 cut(s) 116, 970
Bsp143I GATC 4 cut(s) 47, 593, 782, 919
BspACI CCGC 1 cut(s) 98
BspANI GGCC 1 cut(s) 514
BspCNI CTCAG 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 49
BspMI ACCTGC 1 cut(s) 743
BspPI GGATC 4 cut(s) 42, 55, 601, 790
BsrI ACTGG 2 cut(s) 656, 898
BssECI CCNNGG 2 cut(s) 186, 409
BssMI GATC 4 cut(s) 47, 593, 782, 919
BssT1I CCWWGG 1 cut(s) 186
Bst4CI ACNGT 3 cut(s) 255, 410, 571
BstDEI CTNAG 5 cut(s) 28, 144, 309, 631, 971
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 4 cut(s) 17, 53, 138, 559
BstH2I RGCGCY 1 cut(s) 368
BstHHI GCGC 2 cut(s) 367, 865
BstKTI GATC 4 cut(s) 50, 596, 785, 922
BstMBI GATC 4 cut(s) 47, 593, 782, 919
BstNSI RCATGY 2 cut(s) 274, 542
BstSCI CCNGG 1 cut(s) 728
BstX2I RGATCY 3 cut(s) 47, 593, 782
BstYI RGATCY 3 cut(s) 47, 593, 782
BsuI GTATCC 1 cut(s) 113
BsuRI GGCC 1 cut(s) 514
BtgI CCRYGG 1 cut(s) 409
BtgZI GCGATG 1 cut(s) 649
BtsCI GGATG 4 cut(s) 17, 53, 138, 559
BveI ACCTGC 1 cut(s) 743
CfoI GCGC 2 cut(s) 367, 865
Csp6I GTAC 1 cut(s) 567
CviAII CATG 8 cut(s) 207, 271, 374, 539, 748, 762, 833, 901
CviQI GTAC 1 cut(s) 567
DdeI CTNAG 5 cut(s) 28, 144, 309, 631, 971
DpnI GATC 4 cut(s) 49, 595, 784, 921
DpnII GATC 4 cut(s) 47, 593, 782, 919
DraI TTTAAA 1 cut(s) 561
DraIII CACNNNGTG 1 cut(s) 732
Ecl136II GAGCTC 2 cut(s) 114, 968
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 2 cut(s) 116, 970
Eco53kI GAGCTC 2 cut(s) 114, 968
Eco57I CTGAAG 1 cut(s) 498
EcoICRI GAGCTC 2 cut(s) 114, 968
EcoRI GAATTC 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 2 cut(s) 116, 970
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 8 cut(s) 210, 274, 377, 542, 751, 765, 836, 904
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 410, 442
FaqI GGGAC 2 cut(s) 57, 549
FatI CATG 8 cut(s) 206, 270, 373, 538, 747, 761, 832, 900
FauI CCCGC 1 cut(s) 91
FokI GGATG 4 cut(s) 4, 40, 125, 566
FriOI GRGCYC 2 cut(s) 116, 970
FspBI CTAG 1 cut(s) 684
FspI TGCGCA 1 cut(s) 864
GlaI GCGC 2 cut(s) 366, 864
GsaI CCCAGC 1 cut(s) 128
HaeII RGCGCY 1 cut(s) 368
HaeIII GGCC 1 cut(s) 514
HapII CCGG 1 cut(s) 730
HhaI GCGC 2 cut(s) 367, 865
Hin1II CATG 8 cut(s) 210, 274, 377, 542, 751, 765, 836, 904
Hin6I GCGC 2 cut(s) 365, 863
HinP1I GCGC 2 cut(s) 365, 863
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HindIII AAGCTT 1 cut(s) 873
HinfI GANTC 3 cut(s) 467, 601, 897
HpaI GTTAAC 1 cut(s) 319
HpaII CCGG 1 cut(s) 730
HphI GGTGA 2 cut(s) 243, 833
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 2 cut(s) 478, 779
Hpy188III TCNNGA 3 cut(s) 229, 917, 980
Hpy8I GTNNAC 1 cut(s) 319
HpyAV CCTTC 1 cut(s) 378
HpyCH4III ACNGT 3 cut(s) 255, 410, 571
HpyCH4V TGCA 6 cut(s) 167, 270, 334, 707, 791, 904
HpyF3I CTNAG 5 cut(s) 28, 144, 309, 631, 971
Hsp92II CATG 8 cut(s) 210, 274, 377, 542, 751, 765, 836, 904
HspAI GCGC 2 cut(s) 365, 863
KspAI GTTAAC 1 cut(s) 319
Kzo9I GATC 4 cut(s) 47, 593, 782, 919
LmnI GCTCC 1 cut(s) 111
LpnPI CCDG 7 cut(s) 110, 309, 456, 669, 738, 743, 879
LweI GCATC 2 cut(s) 343, 716
MaeI CTAG 1 cut(s) 684
MaeIII GTNAC 2 cut(s) 641, 889
MalI GATC 4 cut(s) 49, 595, 784, 921
MboI GATC 4 cut(s) 47, 593, 782, 919
MboII GAAGA 3 cut(s) 336, 596, 914
MflI RGATCY 3 cut(s) 47, 593, 782
MhlI GDGCHC 2 cut(s) 116, 970
MluCI AATT 7 cut(s) 78, 168, 396, 448, 500, 837, 988
MlyI GAGTC 1 cut(s) 461
MnlI CCTC 8 cut(s) 486, 525, 618, 656, 773, 790, 969, 976
MroXI GAANNNNTTC 2 cut(s) 288, 828
MseI TTAA 6 cut(s) 171, 294, 318, 560, 840, 987
MslI CAYNNNNRTG 3 cut(s) 205, 543, 766
MspI CCGG 1 cut(s) 730
MspR9I CCNGG 1 cut(s) 730
NciI CCSGG 1 cut(s) 730
NdeII GATC 4 cut(s) 47, 593, 782, 919
NlaIII CATG 8 cut(s) 210, 274, 377, 542, 751, 765, 836, 904
NlaIV GGNNCC 1 cut(s) 49
NmuCI GTSAC 1 cut(s) 641
NsbI TGCGCA 1 cut(s) 864
NspI RCATGY 2 cut(s) 274, 542
PciI ACATGT 1 cut(s) 538
PdmI GAANNNNTTC 2 cut(s) 288, 828
PfeI GAWTC 2 cut(s) 601, 897
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
PscI ACATGT 1 cut(s) 538
Psp124BI GAGCTC 2 cut(s) 116, 970
PspFI CCCAGC 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 49
PsuI RGATCY 3 cut(s) 47, 593, 782
RsaI GTAC 1 cut(s) 568
RsaNI GTAC 1 cut(s) 567
RseI CAYNNNNRTG 3 cut(s) 205, 543, 766
SacI GAGCTC 2 cut(s) 116, 970
SaqAI TTAA 6 cut(s) 171, 294, 318, 560, 840, 987
Sau3AI GATC 4 cut(s) 47, 593, 782, 919
SchI GAGTC 1 cut(s) 461
ScrFI CCNGG 1 cut(s) 730
SduI GDGCHC 2 cut(s) 116, 970
SfaNI GCATC 2 cut(s) 343, 716
SmiI ATTTAAAT 1 cut(s) 561
SmiMI CAYNNNNRTG 3 cut(s) 205, 543, 766
SmlI CTYRAG 1 cut(s) 980
SmoI CTYRAG 1 cut(s) 980
Sse9I AATT 7 cut(s) 78, 168, 396, 448, 500, 837, 988
SsiI CCGC 1 cut(s) 98
SspMI CTAG 1 cut(s) 684
SstI GAGCTC 2 cut(s) 116, 970
StyD4I CCNGG 1 cut(s) 728
StyI CCWWGG 1 cut(s) 186
SwaI ATTTAAAT 1 cut(s) 561
TaaI ACNGT 3 cut(s) 255, 410, 571
TaqI TCGA 1 cut(s) 925
TasI AATT 7 cut(s) 78, 168, 396, 448, 500, 837, 988
TfiI GAWTC 2 cut(s) 601, 897
Tru1I TTAA 6 cut(s) 171, 294, 318, 560, 840, 987
Tru9I TTAA 6 cut(s) 171, 294, 318, 560, 840, 987
TseFI GTSAC 1 cut(s) 641
Tsp45I GTSAC 1 cut(s) 641
TspDTI ATGAA 6 cut(s) 195, 235, 705, 710, 821, 889
XapI RAATTY 2 cut(s) 78, 500
XceI RCATGY 2 cut(s) 274, 542
XmnI GAANNNNTTC 2 cut(s) 288, 828
XspI CTAG 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.