Rmu_sc0002352.1_g000019

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002352.1
OG0031206
Physical Location & Seq
Reverse (-)
91057 .. 91785
729 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
atgatatccttgttcgagttatcgaatgcagccacaacttataatgtgatgaaatatggtgcaaaagcagatgggaaaacagactcaacccaagcatttgtcaagacatgggcagcagcgtgcagctctgctcagacagccaccatgtacgttcccaaaggaaggttcttgctaaaagctgcagtgttcagaggcccttgcaagagtagaattaacgttcagatatacggaaccctgattgcccctactgattattgggctctgggaaactccggctattggatcttattcattaaggtcaatagacttgcagttttcggtggtacactcgacgccaagggagccggcttttgggcttgcaggaaatccggaaagagctgccctgttggagctaggtcgataacgttcaattgggcaaatgacgttgtgattagcggtttagtatccatcaacagtcagcaaactcatcttgtgatcaacagctgcaacaatgtagcggtcagaaacgttaagctggttgctccggatgacagccccaacactgatggcattcatgtgcagagctcaactggtgttactatcactggcgcaaccagcttaacgtttctgaccgctacattgttgcagctgttgatcacaagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

214

Amino Acids

22.9

Weight (kDa)

9.68

Isoelectric Point (pI)

23.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 15 - 194 5.5e-11 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 41 - 198 1.7e-31 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031206)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8338829.1_g000001 Rmu_sc0002352.1_g000019

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 42
AccIII TCCGGA 2 cut(s) 368, 523
AciI CCGC 3 cut(s) 435, 497, 612
AclI AACGTT 4 cut(s) 216, 404, 507, 602
AclWI GGATC 1 cut(s) 290
AcyI GRCGYC 1 cut(s) 333
AfaI GTAC 2 cut(s) 149, 325
AfiI CCNNNNNNNGG 3 cut(s) 162, 279, 351
AgsI TTSAA 1 cut(s) 409
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 9 cut(s) 126, 179, 378, 392, 483, 514, 564, 597, 628
AluI AGCT 9 cut(s) 126, 179, 378, 392, 483, 514, 564, 597, 628
Alw21I GWGCWC 1 cut(s) 566
AlwI GGATC 1 cut(s) 290
Aor13HI TCCGGA 2 cut(s) 368, 523
AoxI GGCC 1 cut(s) 193
ApeKI GCWGC 8 cut(s) 29, 113, 116, 123, 179, 378, 483, 625
AspLEI GCGC 1 cut(s) 590
AspS9I GGNCC 1 cut(s) 194
BanII GRGCYC 2 cut(s) 262, 566
Bbv12I GWGCWC 1 cut(s) 566
BbvI GCAGC 8 cut(s) 41, 125, 128, 135, 166, 365, 470, 637
BccI CCATC 3 cut(s) 65, 455, 539
BciVI GTATCC 1 cut(s) 454
BclI TGATCA 2 cut(s) 474, 633
BfaI CTAG 1 cut(s) 393
BfmI CTRYAG 1 cut(s) 180
BfuI GTATCC 1 cut(s) 454
BisI GCNGC 8 cut(s) 30, 114, 117, 124, 180, 379, 484, 626
BlsI GCNGC 8 cut(s) 31, 115, 118, 125, 181, 380, 485, 627
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 2 cut(s) 232, 343
BsaHI GRCGYC 1 cut(s) 333
BsaJI CCNNGG 1 cut(s) 336
BsaWI WCCGGW 2 cut(s) 368, 523
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 279, 351
Bse118I RCCGGY 1 cut(s) 344
Bse1I ACTGG 2 cut(s) 574, 589
BseAI TCCGGA 2 cut(s) 368, 523
BseDI CCNNGG 1 cut(s) 336
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 3 cut(s) 162, 279, 351
BseMII CTCAG 1 cut(s) 146
BseNI ACTGG 2 cut(s) 574, 589
BseXI GCAGC 8 cut(s) 41, 125, 128, 135, 166, 365, 470, 637
BsgI GTGCAG 2 cut(s) 142, 578
BshFI GGCC 1 cut(s) 195
BsiHKAI GWGCWC 1 cut(s) 566
BsiSI CCGG 4 cut(s) 273, 345, 369, 524
BslI CCNNNNNNNGG 3 cut(s) 162, 279, 351
BsmI GAATGC 2 cut(s) 31, 549
BsnI GGCC 1 cut(s) 195
Bsp1286I GDGCHC 2 cut(s) 262, 566
Bsp13I TCCGGA 2 cut(s) 368, 523
Bsp143I GATC 3 cut(s) 282, 474, 633
BspACI CCGC 3 cut(s) 435, 497, 612
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 1 cut(s) 145
BspEI TCCGGA 2 cut(s) 368, 523
BspLI GGNNCC 2 cut(s) 232, 343
BspMAI CTGCAG 1 cut(s) 184
BspPI GGATC 1 cut(s) 290
BsrFI RCCGGY 1 cut(s) 344
BsrI ACTGG 2 cut(s) 574, 589
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 3 cut(s) 282, 474, 633
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 455
BstACI GRCGYC 1 cut(s) 333
BstC8I GCNNGC 3 cut(s) 121, 346, 358
BstDEI CTNAG 1 cut(s) 132
BstF5I GGATG 1 cut(s) 532
BstHHI GCGC 1 cut(s) 590
BstKTI GATC 3 cut(s) 285, 477, 636
BstMBI GATC 3 cut(s) 282, 474, 633
BstMWI GCNNNNNNNGC 3 cut(s) 137, 341, 594
BstSFI CTRYAG 1 cut(s) 180
BstV1I GCAGC 8 cut(s) 41, 125, 128, 135, 166, 365, 470, 637
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
BsuI GTATCC 1 cut(s) 454
BsuRI GGCC 1 cut(s) 195
BtsCI GGATG 1 cut(s) 532
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 3 cut(s) 189, 540, 582
Cac8I GCNNGC 3 cut(s) 121, 346, 358
CfoI GCGC 1 cut(s) 590
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 194
CseI GACGC 1 cut(s) 341
Csp6I GTAC 2 cut(s) 148, 324
CviAII CATG 3 cut(s) 108, 145, 554
CviQI GTAC 2 cut(s) 148, 324
DdeI CTNAG 1 cut(s) 132
DpnI GATC 3 cut(s) 284, 476, 635
DpnII GATC 3 cut(s) 282, 474, 633
Ecl136II GAGCTC 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 2 cut(s) 262, 566
Eco32I GATATC 1 cut(s) 6
Eco53kI GAGCTC 1 cut(s) 564
EcoICRI GAGCTC 1 cut(s) 564
EcoO109I RGGNCCY 1 cut(s) 194
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 336
EcoT38I GRGCYC 2 cut(s) 262, 566
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 3 cut(s) 111, 148, 557
FaiI YATR 6 cut(s) 42, 57, 109, 146, 226, 555
FatI CATG 3 cut(s) 107, 144, 553
FbaI TGATCA 2 cut(s) 474, 633
Fnu4HI GCNGC 8 cut(s) 30, 114, 117, 124, 180, 379, 484, 626
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 2 cut(s) 262, 566
Fsp4HI GCNGC 8 cut(s) 30, 114, 117, 124, 180, 379, 484, 626
FspBI CTAG 1 cut(s) 393
GlaI GCGC 1 cut(s) 589
GluI GCNGC 8 cut(s) 30, 114, 117, 124, 180, 379, 484, 626
HaeIII GGCC 1 cut(s) 195
HapII CCGG 4 cut(s) 273, 345, 369, 524
HgaI GACGC 1 cut(s) 341
HhaI GCGC 1 cut(s) 590
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 3 cut(s) 111, 148, 557
Hin6I GCGC 1 cut(s) 588
HinP1I GCGC 1 cut(s) 588
HinfI GANTC 1 cut(s) 83
HpaII CCGG 4 cut(s) 273, 345, 369, 524
Hpy166II GTNNAC 1 cut(s) 326
Hpy188I TCNGA 5 cut(s) 135, 191, 222, 503, 609
Hpy188III TCNNGA 3 cut(s) 103, 369, 524
Hpy8I GTNNAC 1 cut(s) 326
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 156
HpyCH4III ACNGT 1 cut(s) 455
HpyCH4IV ACGT 6 cut(s) 150, 216, 404, 423, 507, 602
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 341, 594
HpyF3I CTNAG 1 cut(s) 132
HpySE526I ACGT 6 cut(s) 150, 216, 404, 423, 507, 602
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 3 cut(s) 111, 148, 557
HspAI GCGC 1 cut(s) 588
Kpn2I TCCGGA 2 cut(s) 368, 523
KroI GCCGGC 1 cut(s) 344
KroNI GCCGGC 1 cut(s) 346
Ksp22I TGATCA 2 cut(s) 474, 633
Kzo9I GATC 3 cut(s) 282, 474, 633
LmnI GCTCC 3 cut(s) 341, 389, 526
Lsp1109I GCAGC 8 cut(s) 41, 125, 128, 135, 166, 365, 470, 637
MaeI CTAG 1 cut(s) 393
MaeII ACGT 6 cut(s) 150, 216, 404, 423, 507, 602
MaeIII GTNAC 1 cut(s) 574
MalI GATC 3 cut(s) 284, 476, 635
MboI GATC 3 cut(s) 282, 474, 633
MfeI CAATTG 1 cut(s) 409
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 2 cut(s) 262, 566
MluCI AATT 2 cut(s) 210, 409
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 367
MnlI CCTC 1 cut(s) 185
MroI TCCGGA 2 cut(s) 368, 523
MroNI GCCGGC 1 cut(s) 344
MseI TTAA 4 cut(s) 213, 294, 510, 599
MslI CAYNNNNRTG 1 cut(s) 554
MspA1I CMGCKG 2 cut(s) 483, 628
MspI CCGG 4 cut(s) 273, 345, 369, 524
MunI CAATTG 1 cut(s) 409
Mva1269I GAATGC 2 cut(s) 31, 549
MwoI GCNNNNNNNGC 3 cut(s) 137, 341, 594
NaeI GCCGGC 1 cut(s) 346
NdeII GATC 3 cut(s) 282, 474, 633
NgoMIV GCCGGC 1 cut(s) 344
NlaIII CATG 3 cut(s) 111, 148, 557
NlaIV GGNNCC 2 cut(s) 232, 343
PctI GAATGC 2 cut(s) 31, 549
PdiI GCCGGC 1 cut(s) 346
PkrI GCNGC 8 cut(s) 31, 115, 118, 125, 181, 380, 485, 627
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
PsiI TTATAA 1 cut(s) 42
Psp124BI GAGCTC 1 cut(s) 566
Psp1406I AACGTT 4 cut(s) 216, 404, 507, 602
PspN4I GGNNCC 2 cut(s) 232, 343
PspPI GGNCC 1 cut(s) 194
PstI CTGCAG 1 cut(s) 184
PsuI RGATCY 1 cut(s) 282
PvuII CAGCTG 2 cut(s) 483, 628
RsaI GTAC 2 cut(s) 149, 325
RsaNI GTAC 2 cut(s) 148, 324
RseI CAYNNNNRTG 1 cut(s) 554
SacI GAGCTC 1 cut(s) 566
SaqAI TTAA 4 cut(s) 213, 294, 510, 599
SatI GCNGC 8 cut(s) 30, 114, 117, 124, 180, 379, 484, 626
Sau3AI GATC 3 cut(s) 282, 474, 633
Sau96I GGNCC 1 cut(s) 194
SchI GAGTC 1 cut(s) 77
SduI GDGCHC 2 cut(s) 262, 566
SfcI CTRYAG 1 cut(s) 180
SmiMI CAYNNNNRTG 1 cut(s) 554
Sse9I AATT 2 cut(s) 210, 409
SsiI CCGC 3 cut(s) 435, 497, 612
SspMI CTAG 1 cut(s) 393
SstI GAGCTC 1 cut(s) 566
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 1 cut(s) 455
TaiI ACGT 6 cut(s) 153, 219, 407, 426, 510, 605
TaqI TCGA 4 cut(s) 15, 23, 330, 398
TasI AATT 2 cut(s) 210, 409
Tru1I TTAA 4 cut(s) 213, 294, 510, 599
Tru9I TTAA 4 cut(s) 213, 294, 510, 599
TscAI CASTG 3 cut(s) 189, 547, 589
TseI GCWGC 8 cut(s) 29, 113, 116, 123, 179, 378, 483, 625
TspDTI ATGAA 3 cut(s) 65, 280, 542
TspGWI ACGGA 1 cut(s) 243
TspRI CASTG 3 cut(s) 189, 547, 589
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.